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Master Dissertation Project
Master Dissertation Project

... emergence of drug resistance and the deadly synergism with HIV infection have limited the success of TB disease management. One of the major limitations is the discovery of adequate specific biomarkers for rapid and early diagnosis of active TB, extra-pulmonary TB, latent TB infection (LTBI) and tub ...
10.3 Protein Synthesis
10.3 Protein Synthesis

... • The language of mRNA is called the Genetic Code (A, G, U, C)  (contains only 4 letters) • It is the matching of the RNA sequence to the correct amino acid to make proteins. • It is based on codons, which are 3 bases together on an mRNA chain. • Each codon codes for a specific amino acid • There a ...
Supplementary Table S2 (doc 37K)
Supplementary Table S2 (doc 37K)

... W312 residue lays at the entrance of the active site cavity. In particular, the analysis predicted that this aromatic residue may form a  stacking interaction with the guanidine group of the residue R285. Comparing the structures of the apo-enzyme to the one of the protein bound to N-butyl-deoxyn ...
8 M Guanidine Hydrochloride Solution Buffered, pH - Sigma
8 M Guanidine Hydrochloride Solution Buffered, pH - Sigma

... phosphates, or carboxyl groups, and therefore, is compatible with mass spectrometric procedures. Guanidine hydrochloride is commonly used as a denaturant, because of its ability to break hydrogen bonds between amino acid residues. By breaking these bonds, the 3D conformation of the protein is unfold ...
26.3 Synthesis of Amino Acids
26.3 Synthesis of Amino Acids

... nitrogen in histidine is basic. • The pyrrole-like singly bonded nitrogen is nonbasic because its lone pair of electrons is part of the 6  electron aromatic imidazole ring (see Section 24.4). ...
Sample pages 2 PDF
Sample pages 2 PDF

... it should be recognized that if one uses enough gaps, ultimately any two sequences can be aligned, therefore gap penalties must be assigned. The gap penalties are typically a combination of both the gap number and the size of the gaps. The former are usually penalized more heavily than gap size beca ...
Srivastava, Sanjay: Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition
Srivastava, Sanjay: Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition

... used to identify close homologues that are then aligned to generate a weighted profile formalized as a position-specific scoring matrix (PSSM) (Henikoff & Henikoff, 1994). The Psi-BLAST searches are more sensitive than pairwise sequence homologies search techniques and are widely used. The reason be ...
List of topics - bio.utexas.edu
List of topics - bio.utexas.edu

... Steps in the import of proteins from the cytosol to the mitochondrial matrix. The TOM complex first transports the signal sequence across the outer membrane to the intermembrane space, where it binds to a TIM complex, opening the channel in the complex. The polypeptide chain then either enters the m ...
Direct-Coupling Analysis (DCA)
Direct-Coupling Analysis (DCA)

... What is Molecular coevolution? Two (or more) genes/ proteins/ residues : 1) exert selective pressures on each other 2) evolve in response to each other • Molecular co-evolution can be due to specific co-adaptation between the two co-evolving elements, where changes in one of them are compensated by ...
A Review on Phylogenetic Analysis
A Review on Phylogenetic Analysis

... on protein-coding DNA sequences. Nucleotide substitutions in the coding genes of amino acids of proteins can be either synonymous where amino acid changes or non-synonymous i.e., silent substitutions where amino acid remains same. Usually, most non-synonymous changes would be expected to be eliminat ...
Begrebet ”økologi” er i sig selv ikke ensbetydende med en bedre
Begrebet ”økologi” er i sig selv ikke ensbetydende med en bedre

... source is not defined as organic and is as well a limited resource. Therefore, it is necessary to replace as much as possible of the fish meal protein with competitive organic vegetable protein sources with high protein content as well as a relevant amino acid composition compared to that in fish me ...
Proteins
Proteins

... intake should be not greater than normal. B. Because it takes longer to digest proteins, proteins should be consumed about 3 hours prior to competition. X. What type, how much, and when should protein be consumed during exercise? A. Proteins provide relatively little energy compared to fats and carb ...
Protein Structure
Protein Structure

... state depends strongly on its local environment. This feature is often exploited and histidine is used as a molecular switch. ...
Bio301 Biochemistry I
Bio301 Biochemistry I

... At high ionic strengths the solubilities ofproteins as well as those of most other substances, decrease. This effect is known as salting out. You have given 1.0 M solutions of NaCl, (NH4) 2SO4 and K3PO4.In which of these solutions would you expect that a protein be most soluble? Least soluble? ...
pattern matching
pattern matching

... /M: SY='T'; M= -5,-15,-20,-17,-12,-10,-22,-18, 2,-13, -1, 0,-13, -6,-10,-13, -5, 4, 1,-23, -9,-12; /M: SY='E'; M= -6, -6,-22, -6, 9,-13,-21, -9,-11, 0, -8, -7, -7,-13, 1, 1, -4, -3, -8,-24,-10, 4; /M: SY='E'; M= -5, 9,-24, 11, 15,-24,-12, -3,-23, 3,-20,-15, 6, -9, 5, 1, 4, -2,-19,-29,-16, 9; /M: SY= ...
Mutagenesis of human papillomavirus types 6 and 16 E7 open
Mutagenesis of human papillomavirus types 6 and 16 E7 open

... Mr 3K less. This observation suggests that the amino acid that occupies residue 4 of the peptide chain is a critical determinant of the characteristic migration of the HPV-6 and -16 E7 proteins. The change in the electrophoretic behaviour of the mutated proteins may be related to the negative charge ...
MSAs, SSPs, DBSs and a PHD
MSAs, SSPs, DBSs and a PHD

... For database of N proteins, each time take out one test sequence and leave N-1 proteins for training. After training, use the one test sequence to asses the performance of the method, for example using the Q3 score. Repeat this ...
Protein Translation
Protein Translation

... Three codons serve as stop signs: UAA, UAG or UGA ...
Step 1 - Template Recognition and Initial Alignment
Step 1 - Template Recognition and Initial Alignment

... of the 20 amino acids ought to be aligned. Exchanges between different residues with similar physico-chemical properties (for example F->Y) get a better score than exchanges between residues that widely differ in their properties. Conserved residues generally obtain the highest score. • An alignment ...
Hidden Markov Model for protein secondary structure
Hidden Markov Model for protein secondary structure

... The data set is a subset of 2530 structural domains taken from ASTRAL 1.65 [Brenner et al., 2000], determined by X-ray, with a resolution factor less than 2.25 Å and less than 25% sequence identity. Secondary structure definition is given by an assignment method developed in our laboratory (manuscr ...
The pattern
The pattern

... Yes ! ...
PDF
PDF

... resonance [24]. The en homeodomain contains 3 ¢~helices and an N.terminal arm, Helices l and 2 pack against each other in an antiparallei arrangement and make few contacts with the DNA; helix 3 lies perpendicular to helices I and 2 and, as the 'recognition helix', makes extensive contacts with tile ...
Rabbit anti-Occludin (N-term)
Rabbit anti-Occludin (N-term)

... Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indic ...
Proteins and Electrophoresis
Proteins and Electrophoresis

... – Lys R=(CH2)4NH3+ – Arg R= (CH2)3NHC(NH2)2+ – His R: ...
Last update: 06/22/2015
Last update: 06/22/2015

... similarity (at the nucleotide or protein level) between a query (your input) sequence and sequences within a database. BLAST uses a robust statistical framework that determines if the alignment between two sequences is statistically significant (i.e. has a low probability of the reported alignment b ...
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Ancestral sequence reconstruction

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