pGLO2011 Wilkes
... brilliant green color under UV light. You will be moving the gene for GFP into the E. Coli with the plasmid pGLO. This plasmid encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin. The gene for GFP can be turned on in transformed cells by adding the sugar arabinose, to the ...
... brilliant green color under UV light. You will be moving the gene for GFP into the E. Coli with the plasmid pGLO. This plasmid encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin. The gene for GFP can be turned on in transformed cells by adding the sugar arabinose, to the ...
Lecture: How do neurons work
... Restriction enzymes, also called restriction nucleases (EcoRI in this example), surround the DNA molecule at the point they seek (sequence GAATTC). They cut one strand of the DNA double helix at one point and the second strand at a different, complementary point (between the G and the A base). The s ...
... Restriction enzymes, also called restriction nucleases (EcoRI in this example), surround the DNA molecule at the point they seek (sequence GAATTC). They cut one strand of the DNA double helix at one point and the second strand at a different, complementary point (between the G and the A base). The s ...
Macromolecule Notes Powerpoint
... • 3) Tertiary Structure: The coils and pleats now start folding in on each other. • KEY: shape is maintained by bonding at the R group level! The hydrophobic amino acids will cause the protein to bend (during folding) away from the water. The hydrophilic ones won’t be bothered in the presence of wat ...
... • 3) Tertiary Structure: The coils and pleats now start folding in on each other. • KEY: shape is maintained by bonding at the R group level! The hydrophobic amino acids will cause the protein to bend (during folding) away from the water. The hydrophilic ones won’t be bothered in the presence of wat ...
Macromolecule Notes - Ms. Dooley`s Science Class
... • 3) Tertiary Structure: The coils and pleats now start folding in on each other. • KEY: shape is maintained by bonding at the R group level! The hydrophobic amino acids will cause the protein to bend (during folding) away from the water. The hydrophilic ones won’t be bothered in the presence of wat ...
... • 3) Tertiary Structure: The coils and pleats now start folding in on each other. • KEY: shape is maintained by bonding at the R group level! The hydrophobic amino acids will cause the protein to bend (during folding) away from the water. The hydrophilic ones won’t be bothered in the presence of wat ...
Macromolecules
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
DNA Structure and history10
... Anti-parallel strands • DNA backbone is bonded from phosphate to sugar between 3 & 5 carbons ...
... Anti-parallel strands • DNA backbone is bonded from phosphate to sugar between 3 & 5 carbons ...
CHAPTER 5 THE STRUCTURE AND FUNCTION OF
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
Unit 2 Biochemistry Chp 5 Macromolecules Notes
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
... A protein is a biologically functional molecule that consists of one or more polypeptides folded and coiled into a specific conformation. ...
Study Guide for DNA Structure and Replication
... 1.2.6 Understand cellular structures, their functions, and how specific genes regulate these functions. Describe how DNA molecules are long chains linking four kinds of smaller molecules, whose sequence encodes genetic information. To be successful a student should be able to check off the followi ...
... 1.2.6 Understand cellular structures, their functions, and how specific genes regulate these functions. Describe how DNA molecules are long chains linking four kinds of smaller molecules, whose sequence encodes genetic information. To be successful a student should be able to check off the followi ...
Human genetics
... Normal human nucleated cells contain 46 chromosomes arranged in 22 homologous pairs of autosomal chromosomes in addition to one pair of sex chromosomes that could be similar (i.e. XX) or different (i.e. XY). This arrangement into pairs, based on the position of the centromere and on the length of th ...
... Normal human nucleated cells contain 46 chromosomes arranged in 22 homologous pairs of autosomal chromosomes in addition to one pair of sex chromosomes that could be similar (i.e. XX) or different (i.e. XY). This arrangement into pairs, based on the position of the centromere and on the length of th ...
Mitochondria
... Mitochondrial DNA • Codes for only 37 genes (13 for proteins, 22 for tRNA and 2 for rRNA) • other genes are in the eukaryotic nucleus → posttranslational transport • the two strands are different by their nucleotide content (one strand is guanine-rich, other strand is cytosine-rich) • both of the s ...
... Mitochondrial DNA • Codes for only 37 genes (13 for proteins, 22 for tRNA and 2 for rRNA) • other genes are in the eukaryotic nucleus → posttranslational transport • the two strands are different by their nucleotide content (one strand is guanine-rich, other strand is cytosine-rich) • both of the s ...
Pediatrics-Embryology
... i. The allantois forms- has a respiratory function and acts as a storage sac for urine during embryonic life i. The placenta take over its function very rapidly and the allantois becomes the urachus which ultimately becomes the median umbilical ligament ii. The blood vessels of the allantois become ...
... i. The allantois forms- has a respiratory function and acts as a storage sac for urine during embryonic life i. The placenta take over its function very rapidly and the allantois becomes the urachus which ultimately becomes the median umbilical ligament ii. The blood vessels of the allantois become ...
Genomics - FSU Biology - Florida State University
... discriminate structural genes in two ways: 1) based on the local “non-randomness” of a stretch, and 2) based on the known codon usage of a particular life form. The first, the non-randomness test, does not tell us anything about the particular strand or reading frame; however, it does not require a ...
... discriminate structural genes in two ways: 1) based on the local “non-randomness” of a stretch, and 2) based on the known codon usage of a particular life form. The first, the non-randomness test, does not tell us anything about the particular strand or reading frame; however, it does not require a ...
Chapter 13.1 and 13.2 RNA, Ribosomes, and Protein Synthesis
... • Ribosomes use the codons in mRNA to assemble amino acids to a polypeptide chain or protein. • Process of decoding mRNA to protein is “Translation”. – mRNA transcribed (transcription) in nucleus goes to cytoplasm. – On ribosome, translation begins at START codon. – Each codon attracts an anticodon ...
... • Ribosomes use the codons in mRNA to assemble amino acids to a polypeptide chain or protein. • Process of decoding mRNA to protein is “Translation”. – mRNA transcribed (transcription) in nucleus goes to cytoplasm. – On ribosome, translation begins at START codon. – Each codon attracts an anticodon ...
1 Cancer Lab BRCA – Teacher Background on DNA Bioinformatics
... damage response in the event of DNA double strand breaks. BRCA1 and p53, a tumor suppressor protein, are phosphorylated which prevents cell division. Downstream targets of BRCA1 activation include binding to BRCA2, RAD51, and BARD1 to form a complex. This complex promotes G1 and G1/Sphase arrest dur ...
... damage response in the event of DNA double strand breaks. BRCA1 and p53, a tumor suppressor protein, are phosphorylated which prevents cell division. Downstream targets of BRCA1 activation include binding to BRCA2, RAD51, and BARD1 to form a complex. This complex promotes G1 and G1/Sphase arrest dur ...
3D Ribbon-like Model
... Francis Crick and Sydney Brenner determined how the order of nucleotides in DNA encoded amino acid order Codon – block of 3 DNA nucleotides corresponding to an amino acid Introduced single nulcleotide insertions or deletions and looked for mutations ◦ Frameshift mutations ...
... Francis Crick and Sydney Brenner determined how the order of nucleotides in DNA encoded amino acid order Codon – block of 3 DNA nucleotides corresponding to an amino acid Introduced single nulcleotide insertions or deletions and looked for mutations ◦ Frameshift mutations ...
Unit 3_test1
... Frederick Griffith was able to induce a non-pathogenic (harmless) strain of the bacterium Streptococcus pneumoniae to become pathogenic (deadly). Griffith referred to a transforming factor (we know it as DNA) that caused the non-pathogenic bacteria to become pathogenic. Oswald Avery, Colin MacLeod, ...
... Frederick Griffith was able to induce a non-pathogenic (harmless) strain of the bacterium Streptococcus pneumoniae to become pathogenic (deadly). Griffith referred to a transforming factor (we know it as DNA) that caused the non-pathogenic bacteria to become pathogenic. Oswald Avery, Colin MacLeod, ...
All amino acids participate in these reactions at some
... All amino acids participate in these reactions at some point in their catabolism *** This is false; serine and threonine are not transaminated ¾ they are oxidatively deaminated (release NH3) by a dehydratase enzyme to form pyruvate and propionyl coA respectively. The first step in the catabolism of ...
... All amino acids participate in these reactions at some point in their catabolism *** This is false; serine and threonine are not transaminated ¾ they are oxidatively deaminated (release NH3) by a dehydratase enzyme to form pyruvate and propionyl coA respectively. The first step in the catabolism of ...
SEGMENTAL VARIATION
... • Can’t detect translocations and inversions • Resolution still limited by number of probes on the array—typical resolution about 100 kb • Still a fair amount of variability in results depending on exactly which array is used ...
... • Can’t detect translocations and inversions • Resolution still limited by number of probes on the array—typical resolution about 100 kb • Still a fair amount of variability in results depending on exactly which array is used ...
Complications to Mendel: Gene Interactions Lecture starts on next
... Coat color and type are essential characteristics of domestic dog breeds. Although the genetic basis of coat color has been well characterized, relatively little is known about the genes influencing coat growth pattern, length, and curl. We performed genome-wide association studies of more than 1000 ...
... Coat color and type are essential characteristics of domestic dog breeds. Although the genetic basis of coat color has been well characterized, relatively little is known about the genes influencing coat growth pattern, length, and curl. We performed genome-wide association studies of more than 1000 ...
Learning Objectives
... What is the basic structure of an amino acid? What functional groups does it contain? If I were to give you a page with the structures of the amino acids, you should be able to recognize and name the different structures. You should also be able to look at the amino acids’ side chains and determine ...
... What is the basic structure of an amino acid? What functional groups does it contain? If I were to give you a page with the structures of the amino acids, you should be able to recognize and name the different structures. You should also be able to look at the amino acids’ side chains and determine ...
Genes required for B cell development
... were expressed in white blood cells from the patient, the authors interpret their results as indicating that the mutation had a dominant-suppressor effect on B cell development (24). It is interesting to speculate on how this dominant effect might occur and whether there might be other patients with ...
... were expressed in white blood cells from the patient, the authors interpret their results as indicating that the mutation had a dominant-suppressor effect on B cell development (24). It is interesting to speculate on how this dominant effect might occur and whether there might be other patients with ...
Townes-Brocks Syndrome - Humangenetik Freiburg
... characterized by Duane anomaly and radial ray defects, and less commonly by hearing loss and renal position anomalies (see SALL4-Related Disorders). In a few individuals, complete overlap exists between Okihiro syndrome and TBS [Kohlhase et al 2002 , Borozdin et al 2004]. In those individuals, SALL1 ...
... characterized by Duane anomaly and radial ray defects, and less commonly by hearing loss and renal position anomalies (see SALL4-Related Disorders). In a few individuals, complete overlap exists between Okihiro syndrome and TBS [Kohlhase et al 2002 , Borozdin et al 2004]. In those individuals, SALL1 ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.