Identification of Modified Amino Acids by Edman Sequencing
... on various aspects of Edman degradation of proteins and peptides. These studies provide a means for participating laboratories to compare their analyses against a benchmark of those from other laboratories that provide this valuable service. The ABRF ESRG 2005 sample is a continuation of a similar s ...
... on various aspects of Edman degradation of proteins and peptides. These studies provide a means for participating laboratories to compare their analyses against a benchmark of those from other laboratories that provide this valuable service. The ABRF ESRG 2005 sample is a continuation of a similar s ...
TaqMan® SNP Genotyping Assays User Guide
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
... particular level of observation or actions as described below: Note: Provides information that may be of interest or help but is not critical to the use of the product. ...
Identification and Cloning of a Cryptococcal Deacetylase That
... distributed by DID, Milan, Italy) to 200 mi of dialyzable medium. The culture was incubateci under agitation at 30°C for 2 days and used to inoculate 2-liter aliquots of fresh medium to a density of IO4 cells per mi. Cultures were incubated under agitation with an orbitai shaker at 30°C and harveste ...
... distributed by DID, Milan, Italy) to 200 mi of dialyzable medium. The culture was incubateci under agitation at 30°C for 2 days and used to inoculate 2-liter aliquots of fresh medium to a density of IO4 cells per mi. Cultures were incubated under agitation with an orbitai shaker at 30°C and harveste ...
An investigation of sympatric speciation in diploid organisms by
... Simulations demonstrated that sympatric speciation can occur in scenarios based on the DobzhanskyMüller model and that these processes can be facilitated by the intervention of selective predators. ...
... Simulations demonstrated that sympatric speciation can occur in scenarios based on the DobzhanskyMüller model and that these processes can be facilitated by the intervention of selective predators. ...
Molecular characterization of a cluster of at least two
... 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previous ...
... 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previous ...
Molecular characterization of a cluster of at least two
... 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previous ...
... 1982). The DNA was then reprecipitated as described above and redissolved in 5 ml 50 rnM-Tris/HCl, pH 7.5, 1 mM-EDTA. DNA manipulations. The construction of a gene bank from S. salivarius DNA and the isolation and characterization of the GTFpositive clones LA-8 and LA-33 have been described previous ...
c12) United States Patent - Rice Scholarship Home
... NADH. This system of cofactor regeneration has been successfully applied in the production of optically active amino acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Pu ...
... NADH. This system of cofactor regeneration has been successfully applied in the production of optically active amino acids (Galkin eta!., 1997), chiral hydroxy acids, esters, alcohols, and other fine chemicals synthesized by different dehydrogenases (Hummel and Kula, 1989), (Tishkov eta!., 1999). Pu ...
The female-killing chromosome of the silkworm, Bombyx mori, was
... female-killing factor because no female larvae having the Df(pSa+pW+od)Fem chromosome are produced. Initially, to investigate whether the Df(pSa+pW+od)Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(pSa+pW+od)Fem ...
... female-killing factor because no female larvae having the Df(pSa+pW+od)Fem chromosome are produced. Initially, to investigate whether the Df(pSa+pW+od)Fem chromosome contains any region of the W chromosome or not, we analyzed the presence or absence of 12 W-specific RAPD markers. The Df(pSa+pW+od)Fem ...
Identification and first characterization of pairing
... the gut lumen, from where they are excreted and reach the environment. This process causes severe damage and chronic inflammation of the according tissues, as eggs secrete lytic enzymes and serologically active proteins (Ashton et al., 2001; Schramm & Haas, 2010). A number of eggs are accidentally t ...
... the gut lumen, from where they are excreted and reach the environment. This process causes severe damage and chronic inflammation of the according tissues, as eggs secrete lytic enzymes and serologically active proteins (Ashton et al., 2001; Schramm & Haas, 2010). A number of eggs are accidentally t ...
INHIBITING THE p53–MDM2 INTERACTION: AN IMPORTANT
... The p53 tumour suppressor is present at a low concentration in normal cells. Stress, such as hypoxia or DNA damage, causes p53 to accumulate in the nucleus, where it is active1. Depending on the cellular stress and cell type, the activation of p53 can lead to various responses (FIG. 1). For example, ...
... The p53 tumour suppressor is present at a low concentration in normal cells. Stress, such as hypoxia or DNA damage, causes p53 to accumulate in the nucleus, where it is active1. Depending on the cellular stress and cell type, the activation of p53 can lead to various responses (FIG. 1). For example, ...
Production of L-(+) Lactic Acid From Blackstrap Molasses by
... My sincere appreciation goes to Dr. John Battista, Dr. Alan Biel and Dr. Greg Jarosik for spending their valuable time to discuss and review my work. Their advice and constructive criticism made this work possible. Dr. Roger Laine gave me not only his intellectual insight into this work but also he ...
... My sincere appreciation goes to Dr. John Battista, Dr. Alan Biel and Dr. Greg Jarosik for spending their valuable time to discuss and review my work. Their advice and constructive criticism made this work possible. Dr. Roger Laine gave me not only his intellectual insight into this work but also he ...
University of Groningen To dehydrate or not Khusainov, Rustem
... antimicrobially inactive. Only upon cleavage of the leader sequence that encompasses the first 23 amino acids by the dedicated protease NisP, an active molecule is liberated. Although there are data pointing to the existence of a synthetase complex that modifies nisin, such a complex has not been is ...
... antimicrobially inactive. Only upon cleavage of the leader sequence that encompasses the first 23 amino acids by the dedicated protease NisP, an active molecule is liberated. Although there are data pointing to the existence of a synthetase complex that modifies nisin, such a complex has not been is ...
Purification and Characterization of Chorismate
... Purification of Chorismate Synthase from E. gracilis The purification of chorismate synthase starting from 50 g (wet weight) of E. gracilis cells is summarized in Table I. Chorismate synthase activity was not readily detectable in crude extracts of E. gracilis due to the presence of low molecular we ...
... Purification of Chorismate Synthase from E. gracilis The purification of chorismate synthase starting from 50 g (wet weight) of E. gracilis cells is summarized in Table I. Chorismate synthase activity was not readily detectable in crude extracts of E. gracilis due to the presence of low molecular we ...
Pyruvate dehydrogenase - Wageningen UR E
... hollow interior and contain large pores at their faces. Trimeric E2-units form the building blocks of these aggregates, with extensive and strong interactions. The inter-trimer interactions are much weaker. The active site of E2 is located at each interface of 2 E2subunits inatrimer; a3nmlongchannel ...
... hollow interior and contain large pores at their faces. Trimeric E2-units form the building blocks of these aggregates, with extensive and strong interactions. The inter-trimer interactions are much weaker. The active site of E2 is located at each interface of 2 E2subunits inatrimer; a3nmlongchannel ...
Adaptation and Specialization in the Evolution of Bacterial
... and tradeoffs behind the costs of adaptation in the Lenski LTEE [11]. This study used an assay of respiration as a proxy for fitness on a wide range of carbon sources, and tested performance of the evolved strains at different time points up to 20,000 generations. It found that the strains significa ...
... and tradeoffs behind the costs of adaptation in the Lenski LTEE [11]. This study used an assay of respiration as a proxy for fitness on a wide range of carbon sources, and tested performance of the evolved strains at different time points up to 20,000 generations. It found that the strains significa ...
NAGS - BMC Biochemistry
... glutamine, bicarbonate and ATP as substrates [53]. Nacetylglutamate (NAG), which is formed enzymatically by N-acetylglutamate synthase (NAGS; EC 2.3.1.1) from glutamate and acetyl coenzyme A (Figure 1), is an essential allosteric activator of CPSI, and deficiency of NAG results in a block in ureagen ...
... glutamine, bicarbonate and ATP as substrates [53]. Nacetylglutamate (NAG), which is formed enzymatically by N-acetylglutamate synthase (NAGS; EC 2.3.1.1) from glutamate and acetyl coenzyme A (Figure 1), is an essential allosteric activator of CPSI, and deficiency of NAG results in a block in ureagen ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... play important role in the development of gastric diseases such as chronic gastric inflammation, ulcerative peptic diseases, gastric cancer and MALT-lymphoma [1]. There are many virulence factors for H. pylori colonization and infection but CagA is one of the most studied virulence of H. pylori. Thi ...
... play important role in the development of gastric diseases such as chronic gastric inflammation, ulcerative peptic diseases, gastric cancer and MALT-lymphoma [1]. There are many virulence factors for H. pylori colonization and infection but CagA is one of the most studied virulence of H. pylori. Thi ...
Rat sn-glycerol-3-phosphate acyltransferase - UNC
... regarding their use of saturated vs. unsaturated fatty acyl-CoAs? Do they play speci¢c and independent roles in (1) regulating the £ux of acyl-CoAs towards glycerolipid synthesis or towards L-oxidation, (2) in the synthesis of mitochondrial vs. microsomal phospholipids, and (3) in the synthesis of t ...
... regarding their use of saturated vs. unsaturated fatty acyl-CoAs? Do they play speci¢c and independent roles in (1) regulating the £ux of acyl-CoAs towards glycerolipid synthesis or towards L-oxidation, (2) in the synthesis of mitochondrial vs. microsomal phospholipids, and (3) in the synthesis of t ...
Sequence and Structure Classification of Kinases
... ATP-grasp family. An evolutionary link between these three families based on structural similarity has been described.6 In each of the group 1 families, the active site of the enzyme is located in the cleft between two a þ b domains (Figure 1(a)). The protein kinase-like family members and SAICAR sy ...
... ATP-grasp family. An evolutionary link between these three families based on structural similarity has been described.6 In each of the group 1 families, the active site of the enzyme is located in the cleft between two a þ b domains (Figure 1(a)). The protein kinase-like family members and SAICAR sy ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.