Amino acids
... by taking simple molecules and chemically linking them together to make long chains analogous to box cars on a train. Macromolecules may have a complex three dimensional structure. This is especially true for proteins. The three dimensional structure typically relates to the function of the macromol ...
... by taking simple molecules and chemically linking them together to make long chains analogous to box cars on a train. Macromolecules may have a complex three dimensional structure. This is especially true for proteins. The three dimensional structure typically relates to the function of the macromol ...
chapter 3 - Fullfrontalanatomy.com
... the consequences of their own actions? What, if any, distinctions can you make between people choosing to smoke versus people who consume fast food? Should courts be involved in such decisions? What can fast-food companies do to make the “dangers” of their products obvious to the consumers? Should i ...
... the consequences of their own actions? What, if any, distinctions can you make between people choosing to smoke versus people who consume fast food? Should courts be involved in such decisions? What can fast-food companies do to make the “dangers” of their products obvious to the consumers? Should i ...
RNA and Protein Synthesis - Port Washington School District
... – tRNA carrying amino acid pairs with mRNA codon – Usually mRNA at start is AUG (“start codon”) – mRNA slides along ribosome to next codon – New tRNA with amino acid pairs to mRNA codon – Amino acids get joined by enzyme by a peptide bond – Process continues and chain of amino acids form until a sto ...
... – tRNA carrying amino acid pairs with mRNA codon – Usually mRNA at start is AUG (“start codon”) – mRNA slides along ribosome to next codon – New tRNA with amino acid pairs to mRNA codon – Amino acids get joined by enzyme by a peptide bond – Process continues and chain of amino acids form until a sto ...
Use of Far-Red Emitting DNA Dye DRAQ5 for Cell Cycle
... An Acumen eX3 scans on an area and not well basis, thus scan times are virtually identical for any SBS format microplate. Typically plate cycle times of 10 minutes are achievable but these can be further cut by scanning reduced well areas. Several hundred plates can be scanned per day with minimal r ...
... An Acumen eX3 scans on an area and not well basis, thus scan times are virtually identical for any SBS format microplate. Typically plate cycle times of 10 minutes are achievable but these can be further cut by scanning reduced well areas. Several hundred plates can be scanned per day with minimal r ...
Supplementary Information (doc 38K)
... In coimmunoprecipitation assays of endogenous proteins, MCF7 cells were lysed by mild sonication in NET-NB buffer (20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5% NP-40, 150 mM NaCl) and immunoprecipitation was performed using anti-ATF-2 (N96, Santa Cruz Biotech.), anti-BRCA1 (Ab-3, Oncogene Research), or a ...
... In coimmunoprecipitation assays of endogenous proteins, MCF7 cells were lysed by mild sonication in NET-NB buffer (20 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5% NP-40, 150 mM NaCl) and immunoprecipitation was performed using anti-ATF-2 (N96, Santa Cruz Biotech.), anti-BRCA1 (Ab-3, Oncogene Research), or a ...
the chemistry of life - Fall River Public Schools
... Carbon is unparalleled in its ability to form molecules that are large, complex, and diverse. Why? o It has 4 valence electrons. o It can form up to 4 covalent bonds. o These can be single, double, or triple covalent bonds. o It can form large molecules. o These molecules can be chains, ring-shaped, ...
... Carbon is unparalleled in its ability to form molecules that are large, complex, and diverse. Why? o It has 4 valence electrons. o It can form up to 4 covalent bonds. o These can be single, double, or triple covalent bonds. o It can form large molecules. o These molecules can be chains, ring-shaped, ...
33835_CellsBldgBlcks TG
... fat: Substance that supplies the body with energy and also helps support and cushion the vital organs in the body. fermentation: Energy-releasing process that does not require oxygen; less efficient than respiration. heterotroph: Organism that cannot make its own food. homeostasis: Ability of an org ...
... fat: Substance that supplies the body with energy and also helps support and cushion the vital organs in the body. fermentation: Energy-releasing process that does not require oxygen; less efficient than respiration. heterotroph: Organism that cannot make its own food. homeostasis: Ability of an org ...
Supplementary Methods
... oxidase both of the paired samples were incubated with 50mM DMPO (5,5-dimethyl-1-pyrroline Noxide, Sigma), as the electron spin-trap, 100M diethyldithiocarbamate, 300M NAD(P)H (as the substrate for NAD(P)H oxidase) in PBS. One of the paired samples was given 10M of the NAD(P)H oxidase specific in ...
... oxidase both of the paired samples were incubated with 50mM DMPO (5,5-dimethyl-1-pyrroline Noxide, Sigma), as the electron spin-trap, 100M diethyldithiocarbamate, 300M NAD(P)H (as the substrate for NAD(P)H oxidase) in PBS. One of the paired samples was given 10M of the NAD(P)H oxidase specific in ...
Lecture 11 - Horizontal Gene Transfer S11 2 slides per page
... Recipient cell must be competent Natural competence Observed in only certain species Example - Streptococcus pneumoniae (GPC) •Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus ...
... Recipient cell must be competent Natural competence Observed in only certain species Example - Streptococcus pneumoniae (GPC) •Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus ...
Syllabus: AP Bio - Glen Ridge Public Schools
... and to show the process of transcription and translation in protein synthesis using either paper models or computer simulations Extract DNA from a cell culture. Using paper plasmids, demonstrate how restriction endonucleases produce "sticky ends" necessary for many recombinant DNA technologies. Perf ...
... and to show the process of transcription and translation in protein synthesis using either paper models or computer simulations Extract DNA from a cell culture. Using paper plasmids, demonstrate how restriction endonucleases produce "sticky ends" necessary for many recombinant DNA technologies. Perf ...
A significant similarity is the fact that both prokaryotes and
... circular, double strand of DNA. Prokaryotes and some eukaryotes are also known to have additional small satellite structures of DNA called plasmids. Both prokaryotes and eukaryotes also contain ribosomes which are the organelles responsible for accurately translating this linear genetic code (via me ...
... circular, double strand of DNA. Prokaryotes and some eukaryotes are also known to have additional small satellite structures of DNA called plasmids. Both prokaryotes and eukaryotes also contain ribosomes which are the organelles responsible for accurately translating this linear genetic code (via me ...
Lecture 11 - Horizontal Gene Transfer Chapt. 8 S11
... Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In the laboratory, treat cells with specific ...
... Becomes competent in late log phase •Competent cell binds ds DNA •Enzymes cut DNA into smaller fragments (5 - 15 kb) •Single strand is taken up by cell Example - Haemophilus influenzae (GNR) •Cell binds DNA only from related species Artificial competence In the laboratory, treat cells with specific ...
Research Highlights: Highlights from the last year in nanomedicine
... DIds /g m. Consequently, they can monitor the analyte’s binding kinetics in realtime and fit this to rate equations (i.e., a Langmuir isotherm), extracting para meters such as binding affinities and rate constants. To demonstrate the efficacy of their method, Duan and coworkers performed a series o ...
... DIds /g m. Consequently, they can monitor the analyte’s binding kinetics in realtime and fit this to rate equations (i.e., a Langmuir isotherm), extracting para meters such as binding affinities and rate constants. To demonstrate the efficacy of their method, Duan and coworkers performed a series o ...
E. Coli
... COMPETENCE. Ex. Bacillus, Streptococcus, Haemophilus and Neisseria. Some bacterial strains, such as E. coli, can be made ARTIFICIALLY COMPETENT. ...
... COMPETENCE. Ex. Bacillus, Streptococcus, Haemophilus and Neisseria. Some bacterial strains, such as E. coli, can be made ARTIFICIALLY COMPETENT. ...
RECOMBINANT DNA TECHNOLOGY
... are sealed using ligase. The result is recombinant DNA. When this recombinant vector is inserted into E. coli, the cell processes the instructions and by translation & transcription, it assembles the amino acids forming the protein product of interest. More importantly, the new instructions are p ...
... are sealed using ligase. The result is recombinant DNA. When this recombinant vector is inserted into E. coli, the cell processes the instructions and by translation & transcription, it assembles the amino acids forming the protein product of interest. More importantly, the new instructions are p ...
Unifying Principles of Biology
... An organism’s traits are encoded in their DNA, the large molecule, or macromolecule, that holds the instructions needed to build cells and organisms. DNA makes up the genes of an organism. Traits are passed on from one generation to the next by way of these genes. Information for how the organism ap ...
... An organism’s traits are encoded in their DNA, the large molecule, or macromolecule, that holds the instructions needed to build cells and organisms. DNA makes up the genes of an organism. Traits are passed on from one generation to the next by way of these genes. Information for how the organism ap ...
Chapter 11 ~ DNA and the Language of Life
... are now available to form base pairs with their complement from a new free-floating nucleotide. 3. Both original strands can be copied, making two double helices from one original. 4. The new helices are composed of half old (original) and half new nucleotides. ...
... are now available to form base pairs with their complement from a new free-floating nucleotide. 3. Both original strands can be copied, making two double helices from one original. 4. The new helices are composed of half old (original) and half new nucleotides. ...
Cell-penetrating peptide
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular uptake of various molecular cargo (from nanosize particles to small chemical molecules and large fragments of DNA). The ""cargo"" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions. The function of the CPPs are to deliver the cargo into cells, a process that commonly occurs through endocytosis with the cargo delivered to the endosomes of living mammalian cells.CPPs hold great potential as in vitro and in vivo delivery vectors for use in research and medicine. Current use is limited by a lack of cell specificity in CPP-mediated cargo delivery and insufficient understanding of the modes of their uptake.CPPs typically have an amino acid composition that either contains a high relative abundance of positively charged amino acids such as lysine or arginine or has sequences that contain an alternating pattern of polar/charged amino acids and non-polar, hydrophobic amino acids. These two types of structures are referred to as polycationic or amphipathic, respectively. A third class of CPPs are the hydrophobic peptides, containing only apolar residues, with low net chargeor have hydrophobic amino acid groups that are crucial for cellular uptake.The first CPP was discovered independently by two laboratories in 1988, when it was found that the trans-activating transcriptional activator (TAT) from human immunodeficiency virus 1 (HIV-1) could be efficiently taken up from the surrounding media by numerous cell types in culture. Since then, the number of known CPPs has expanded considerably and small molecule synthetic analogues with more effective protein transduction properties have been generated.