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Natural Selection and Evidence to Support Evolution
Natural Selection and Evidence to Support Evolution

... automatic change in population • Due to variation that already exist in a population a change in the environment is going to cause the more beneficial trait to become more dominant • Changes in physical appearance (phenotype) occur when there is a change in the genotype (mutation) and the environmen ...
Topic: Genetics Aim: Describe some methods that can be used to
Topic: Genetics Aim: Describe some methods that can be used to

... Aim: Describe some methods that can be used to develop organisms with desirable traits. Directions: Read the text below. Use textual evidence to answer questions 1-14. Genetic engineering involves biological and chemical methods to change the arrangement of a gene’s DNA. One method of genetic engine ...
Quiz 22
Quiz 22

... C. The genetically modified plants are made to be sterile so that they cannot breed with wild types. D. Antibiotic resistant gene is inserted into the genetically modified plants. 14. There is concern about therapy involving embryonic stem cells because (i) human embryos are destroyed to obtain embr ...
Methods to analyze RNA expression - RNA
Methods to analyze RNA expression - RNA

8.2 Structure of DNA
8.2 Structure of DNA

... more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulation to be discovered. – The lac operon has three genes that code for enzymes that break down lactose. ...
Biochemistry - Bishop Ireton High School
Biochemistry - Bishop Ireton High School

... • In most chemical reactions this AE is in the form of heat • This heat energy moves the reactants called SUBSTRATES around causing them to bump into each other. • In the body, heat can’t be used as AE because it would harm the body. ...
DNA: I`m All Split Up
DNA: I`m All Split Up

... of DNA.” For the second time, there will be a pairing of nucleotides on the screen. Ask, “How is the DNA code transcribed?” (When a special enzyme, RNA polymerase, encounters a coded start signal on the DNA, the doubled stranded DNA molecule is broken apart and the polymerase begins pairing RNA nuc ...
TD7: Gel Electrophoresis Photoaffinity probes GEL
TD7: Gel Electrophoresis Photoaffinity probes GEL

... PAGE= polyacrylamide gel electrophoresis Purpose: resolve proteins by size and no other physical feature (ie charge or shape) Gels: made from polymerized acrylamide, typically 6-20% (higher %= more dense gel, same protein runs slower) Running buffer: has .1% SDS detergent Sample: pre-treated with SD ...
Organization of the eukaryotic genomes
Organization of the eukaryotic genomes

Microarray Protocol
Microarray Protocol

... 6) Discard flow-through and add 2.5ml Buffer RPE, and centrifuge for 2 min. Add another 2.5 ml RPE, and repeat centrifuge for 5 min. 7) Place column in a new 15ml tube and add 250l DEPC water, let stand 1 min and centrifuge for 3 minutes to elute RNA (use only 150l water if sample is less than 150 ...
Disorders associated with mutations in the POLG gene
Disorders associated with mutations in the POLG gene

... Oxford Medical Genetics Labs ...
Due: 2015. 10. 12. 11:00 am (월)
Due: 2015. 10. 12. 11:00 am (월)

... The kinetics of allosteric enzymes usually does not fit on Michaelis-Menten equation because modulator (regulator) that binds to the enzyme changes the activity on the substrate(S). Thus there are two states, R and T state. A model that hypothesizes the existence of equilibrium between the two state ...
DNA Replication
DNA Replication

... This paradox is resolved by the use of Okazaki fragments. These are short, discontinuous replication products that are produced off the lagging strand. This is in comparison to the continuous strand that is made off the leading strand. 4. The final product does not have RNA stretches in it. These ar ...
Final Exam
Final Exam

... green eye and one yellow eye. Cross two hybrid cats. What is the chance that they will have four female cats in a row with green eyes? What is the chance that they will have five male cats that each has one green eye and one yellow eye? Show your work in a Punnett square. 4 female cats with green ey ...
12.6 DNA Repair
12.6 DNA Repair

... energy to split pyrimidine dimers that kink the DNA. Pyrimidine dimers - bonds between C’s and/or T’s on the same strand.  Photolyases - enzymes that absorb light energy and use it to detect and bind to pyrimidine dimers, then break the extra bond.  Humans do not have this type of repair ...
Out-of-Africa Theory: The Origin Of Modern Humans
Out-of-Africa Theory: The Origin Of Modern Humans

...  Mitochondria are structures within cells that convert the energy from food into a form that cells can use. Although most DNA is packaged in chromosomes within the nucleus, mitochondria also have a small amount of their own DNA. This genetic material is known as mitochondrial DNA or mtDNA. In human ...
Biology
Biology

... Using the DNA Sequence DNA polymerase adds complementary strand 1. Reading the Sequence 2. Cutting and Pasting DNA heated to 3. Making Copies separate strands ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... (active) where phosphodiester bonds form – E and A sites partially overlap – Rotation of nucleotide between the E and A sites may play a role in base and sugar specificity ...
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... Lac operon is turned on- as a small amount of lactose is converted to allolactose (inducer)binds repressor and changes its conformation repressor can not bind operator Adenylyl cyclase is actively – in the glucose absencesynthesising cAMP cAMP-CAP complex binds CAP site RNA polymerase initiates ...
RNA codons and correlant Amino Acids
RNA codons and correlant Amino Acids

... DNA codes for every physical attribute we possess. For example, half of your code was in the head of a sperm which fused with your other half of your code in the centre of an egg to form a single cell a single code - the zygote. This single cell then multiplied table.jpg with along with its code - t ...
Slides-Brian_Charlesworth-Sex_and_molecular_evolution
Slides-Brian_Charlesworth-Sex_and_molecular_evolution

... have well-formulated models that can be related to data. • To produce these models, we need to include processes that are likely to be operating in the real world. • Before introducing them, let’s look at some patterns that are revealed by studying DNA sequence variation and evolution. • Differences ...
Proc 16(4) Oct 03 web.indd
Proc 16(4) Oct 03 web.indd

Chapter 1
Chapter 1

... isomer, in which they therefore spend most of their time. However, all four bases can also adopt distinct “tautomeric” conformations. At any time, a proportion of the molecules will explore these less stable conformations. The A-T and G-C base pairing rules d ...
university of leeds - Research Complex at Harwell
university of leeds - Research Complex at Harwell

... Structural Basis of Holliday Junction Resolution Background to the post The posts are supported by an MRC programme grant to Prof. Simon Phillips and Dr. Stephen Carr at RCaH, as part of long-term study of the structure and function of Holliday junction resolvases. The programme involves collaborati ...
modification of gene expression
modification of gene expression

... Why twist & compact? • Space: total length of DNA a single cell is 6 feet • Protection from tangles and breakage ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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