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The lac Operon - kyoussef-mci
The lac Operon - kyoussef-mci

... Since tryptophan is the molecule that determines when the operon is turned on or off, it is known as an effector molecule. corepressor – effector molecule that binds repressor protein to cause it to bind to the operator ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
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... nuclear migration (Oakley and Morris, 1980). These compounds appear to bind tubulin from fungi (Davidse and Flach, 1977; Baum et al., 1978; Kilmartin, 1981) and may have an effect similar to that of the mitotic inhibitors colchicine and colcemid in higher eucaryotic cells. Mutants of Aspergillus sel ...
Biotechnology
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... e. They have two domains but neither domain can be engineered and are therefore not useful to biotechnology research. 8. Which of the following DNA structure modifications are used to regulate transcription? a. acetylation/Deacetylation of the histone tails b. methylation of specific bases in the DN ...
A Rapid Screening Method to Detect Nonsense and Frameshift
A Rapid Screening Method to Detect Nonsense and Frameshift

... normal DNA inserts varied, especially in comparison to the lighter blue color obtained from the mutant inserts. Although it was not difficult to differentiate colors among colonies on a single plate, comparisons from one plate to another were difficult to interpret. Colors obtained from samples with ...
ALAMO HEIGHTS INDEPENDENT SCHOOL DISTRICT ALAMO
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Dynamic epigenetic responses to childhood exposure to violence
Dynamic epigenetic responses to childhood exposure to violence

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Solutions - International Junior Science Olympiad
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pdf - at www.arxiv.org.
pdf - at www.arxiv.org.

... only allows a gain of the weak base pair (AG) for serine. We have to use another short graph, AA←GA→UA, to illustrate the loss of glutamic acid (Glu). This loss generates more questions than answers. The GA→AA replacement must result in lysine enrichment but although lysine is a polar amino acid, it ...
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Diapositiva 1 - Universidad de Sevilla
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... These five proteins likely have very similar secondary and tertiary structures. These five proteins likely perform similar functions in the cell. The genes that encode these five proteins likely have a common evolutionary ancestor. A, B, and C are all correct. This is a remarkable coincidence, but y ...
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Differential mRNA expression levels and gene sequences of a
Differential mRNA expression levels and gene sequences of a

... clone from the resistant strain. This nucleotide change results in an amino acid difference in the predicted protein sequence from tryptophan (Trp220) in the susceptible strain to a glycine (Gly220) in the resistant strain. The point mutation at position 658 was confirmed in the genomic DNA sequence ...
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Cytochrome P450 2D6 (CYP2D6) - St. Jude Children`s Research

... DNA is like a set of instructions for your body that can help decide how well your enzymes will work. Each person differs from another at the DNA (gene) level. This means that each person has small differences in the genes that code for enzymes. The part of DNA that instructs how well the CYP2D6 enz ...
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... one of our most important products in five to ten years” because of the growth in molecular testing, says Colpan. Much of Qiagen’s innovation is prompted by developments in academic science. For example, now the human genome has been sequenced, scientists want to know what each gene does, a process ...
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Topic guide 1.2: Enzymes

... be an increased number of collisions between enzyme and substrate molecules. This in turn will increase the rate of reaction and so the products will be formed more quickly. However, applying too much heat can cause enzymes to denature. The increased vibrations and collisions put strains on the bond ...
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Deoxyribozyme



Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of catalyzing specific chemical reactions, similar to the action of other biological enzymes, such as proteins or ribozymes (enzymes composed of RNA).However, in contrast to the abundance of protein enzymes in biological systems and the discovery of biological ribozymes in the 1980s,there are no known naturally occurring deoxyribozymes.Deoxyribozymes should not be confused with DNA aptamers which are oligonucleotides that selectively bind a target ligand, but do not catalyze a subsequent chemical reaction.With the exception of ribozymes, nucleic acid molecules within cells primarily serve as storage of genetic information due to its ability to form complementary base pairs, which allows for high-fidelity copying and transfer of genetic information. In contrast, nucleic acid molecules are more limited in their catalytic ability, in comparison to protein enzymes, to just three types of interactions: hydrogen bonding, pi stacking, and metal-ion coordination. This is due to the limited number of functional groups of the nucleic acid monomers: while proteins are built from up to twenty different amino acids with various functional groups, nucleic acids are built from just four chemically similar nucleobases. In addition, DNA lacks the 2'-hydroxyl group found in RNA which limits the catalytic competency of deoxyribozymes even in comparison to ribozymes.In addition to the inherent inferiority of DNA catalytic activity, the apparent lack of naturally occurring deoxyribozymes may also be due to the primarily double-stranded conformation of DNA in biological systems which would limit its physical flexibility and ability to form tertiary structures, and so would drastically limit the ability of double-stranded DNA to act as a catalyst; though there are a few known instances of biological single-stranded DNA such as multicopy single-stranded DNA (msDNA), certain viral genomes, and the replication fork formed during DNA replication. Further structural differences between DNA and RNA may also play a role in the lack of biological deoxyribozymes, such as the additional methyl group of the DNA base thymidine compared to the RNA base uracil or the tendency of DNA to adopt the B-form helix while RNA tends to adopt the A-form helix. However, it has also been shown that DNA can form structures that RNA cannot, which suggests that, though there are differences in structures that each can form, neither is inherently more or less catalytic due to their possible structural motifs.
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