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pages 46-50
pages 46-50

... structure of living things and carry out most of the processes that keep organisms alive. Carbon is so important because its atomic structure gives it bonding properties that are unique among elements. Each carbon atom has four unpaired electrons in its outer energy level. Therefore, carbon atoms ca ...
PRO
PRO

... of a given gene locus from genetic variations, alternative splicing, proteolytic cleavage, PTMs => “proteoforms” – Ontology for Protein Complexes (ProComp): Captures distinct complexes as exist in different species and defines through component proteins ...
Transcription and Translation
Transcription and Translation

Semester 1 AP Biology Exam Review Guide Directions: Use this as
Semester 1 AP Biology Exam Review Guide Directions: Use this as

... 5. Steroids (cholesterol and sex hormones) vi. Proteins 1. Draw an amino acid (the monomer of a protein). 2. Give the five major groups of proteins and one example of how each group is used in the body. 3. How are amino acids joined together? 4. Draw and describe the following protein structures. 5. ...
Chapter 9 Membranes, con`t.
Chapter 9 Membranes, con`t.

... hydroxyl groups of serine, threonine or hydroxylysine • N-linked saccharides are attached via the amide nitrogens of asparagine residues • See structures in Figure 9.26 and 9.29 ...
Introduction to Lab Ex. 17: Fermentation of Carbohydrates F
Introduction to Lab Ex. 17: Fermentation of Carbohydrates F

... In the absence of aerobic conditions, organisms may be able to the sugars, though with minimal gain of ATP. These processes are characterized by the end products. Organisms are able to metabolize carbohydrates to harvest the energy in these compounds through metabolic pathways. The ability of cells ...
Comparison between Solid Phase Synthesis and Recombinant
Comparison between Solid Phase Synthesis and Recombinant

... (in-house and CMO) = 1bn USD Market growth = 10 – 15% p.a. Launched Peptides = 43 250 in Clinical Phase >200 in Pre-Clinical Emerging areas: Cancer, HIV, Cardiovascular, CNS and Metabolic disorders Generic peptides ...
Document
Document

Complete nucleotide sequence and genome organization of a
Complete nucleotide sequence and genome organization of a

... The 3’-terminal non-translated region in the cr-TMV genome can be folded into seven potentially stable pseudoknots and one additonal pseudoknot at the 3’-end of the CP gene compared to only five pseudoknots found in the Ul-TMV sequence (Fig. 4). The strongest structural conse~ation between the cr-TM ...
elisa - WordPress.com
elisa - WordPress.com

... Bovine serum albumin (also known as BSA or "Fraction V") • a serum albumin protein that has numerous biochemical applications including ELISA and immunohistochemistry. • It is also used as a nutrient in cell and microbial culture. • In restriction digests, – BSA is used to stabilize some enzymes du ...
biomolecules
biomolecules

... react to form organic molecules, such as amino acids. A competing theory is the RNA-world hypothesis. This hypothesis states that RNA developed first. It may have self-replicated and also served as a template for making DNA. Today, RNA serves as the intermediate between DNA and proteins. If this hypo ...
DNA Synthesis (Replication)
DNA Synthesis (Replication)

... to bridge the genetic code (AUCG) in mRNA with the twenty amino-acids code in proteins. Details of tRNA:  The role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid  One end of the tRNA complements the genetic code in a threenucleotide sequence called the a ...
7-12 Enzyme Demonstration Instructions
7-12 Enzyme Demonstration Instructions

... 2. Next ask what all of these items have in common (answer biocatalysis).  Explain that starchases  are involved in making clothing by breaking down starch used in processing, cellulases involved  in breaking down fruit to get more fruit juice out, rennet  enzymes are used to make cheese,  proteases ...
Amino Acid Catabolism
Amino Acid Catabolism

... Catabolism of amino acids increases  for use in gluconeogenesis when glucose is unavailable (e.g., starvation/diabetes)  when protein content of diet exceeds need for building blocks  during times of stress ...
Direct-Coupling Analysis (DCA)
Direct-Coupling Analysis (DCA)

... What is Molecular coevolution? Two (or more) genes/ proteins/ residues : 1) exert selective pressures on each other 2) evolve in response to each other • Molecular co-evolution can be due to specific co-adaptation between the two co-evolving elements, where changes in one of them are compensated by ...
A novel protein transport system involved in the biogenesis of
A novel protein transport system involved in the biogenesis of

... produce cofactor-containing proteins in the extracellular compartment. Biosynthesis of such extracytoplasmic redox proteins must involve both protein transport across the cytoplasmic membrane and cofactor insertion into the protein. However, combining these two processes presents a severe biosynthet ...
Mutation of exposed hydrophobic amino acids to arginine to
Mutation of exposed hydrophobic amino acids to arginine to

... enzymes up to 300-fold more sensitive [1,2]. But like most enzymes from mesophilic organisms, Drosophila AChE is not stable, and this instability precludes its utilization in biosensors. It can be stabilized by adding some molecules in the solution such as reversible inhibitors, polyethylene glycol ...
Biology 231
Biology 231

... adenine (A) – DNA and RNA guanine (G) – DNA and RNA cytosine (C) – DNA and RNA thymine (T) – DNA only uracil (U) – RNA only nucleic acid strands have backbones of sugars and phosphates joined by dehydration reactions RNA is single-stranded DNA is a double-stranded helix held together by hydrogen bon ...
Protein Structure Analysis
Protein Structure Analysis

... Structures are grouped into fold families at this level depending on both the overall shape and connectivity of the secondary structures. This is done using the structure comparison algorithm SSAP. Some fold families are very highly populated and are currently subdivided using a higher cutoff on the ...
Fatty Acid Spiral
Fatty Acid Spiral

... • Two carbons at a time are cleaved from a fatty acyl-CoA as acetyl-CoA. • This cleavage continues until the entire fatty acid has been converted into acetyl-CoA. ...
Document
Document

... • Two carbons at a time are cleaved from a fatty acyl-CoA as acetyl-CoA. • This cleavage continues until the entire fatty acid has been converted into acetyl-CoA. ...
Acid - Perkins Science
Acid - Perkins Science

Click 1
Click 1

... The aspartic proteases are inhibited by pepstatin. They are also sensitive to diazoketone compounds such as diazoacetyl-D,L-norleucine methyl ester (DAN) and 1,2-epoxy-3-(pnitrophenoxy)propane (EPNP) in the presence of copper ions Cysteine/thiol proteases occur in both prokaryotes and eukaryotes. Ab ...
FindTarget: software for subtractive genome analysis
FindTarget: software for subtractive genome analysis

... environment is a specific property of Helicobacter pylori in comparison to Haemophilus influenzae and E. coli, the resulting list (73 proteins) contains candidate factors possibly required for survival in an acid gastric environment and thus also possible drug targets. To date two complementary in s ...
AutoMotif server: prediction of single residue post-translational
AutoMotif server: prediction of single residue post-translational

... Fig. 1. The AutoMotif server data flowchart for prediction of PTM sites in proteins. The general model of the information flow and components of the automatic annotation service for prediction of post-translational single residue modification sites in proteins. The identification of PTM sites in a q ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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