biomedical therapy
... Because many conventional pharmaceuticals affect enzymes, damage to enzyme systems is frequently iatrogenic in origin. In addition, enzyme activity is impaired by increased environmental loading (e.g., by heavy metals or pesticides). Failure of an enzymatic function causes accumulation of not only m ...
... Because many conventional pharmaceuticals affect enzymes, damage to enzyme systems is frequently iatrogenic in origin. In addition, enzyme activity is impaired by increased environmental loading (e.g., by heavy metals or pesticides). Failure of an enzymatic function causes accumulation of not only m ...
Crystal structure of the S187F variant of human liver alanine
... aldimine intermediate, in which the amino group of Lalanine replaces the E-amino group of Lys209, the PLPbinding residue. Subsequent proton abstraction from Ca yields the quinonoid intermediate, and reprotonation at the C40 of the coenzyme produces the ketimine intermediate. Hydrolysis of the ketimi ...
... aldimine intermediate, in which the amino group of Lalanine replaces the E-amino group of Lys209, the PLPbinding residue. Subsequent proton abstraction from Ca yields the quinonoid intermediate, and reprotonation at the C40 of the coenzyme produces the ketimine intermediate. Hydrolysis of the ketimi ...
Protein Sequence Alignment and Database Searching
... • If they align, they are „similar“ • If they are similar, then they might have similar structure and/or function. Identify conserved patterns (motifs) • If one of them has known structure/function, then alignment of other might yield insight about how the structure/functions works. Similar motif ...
... • If they align, they are „similar“ • If they are similar, then they might have similar structure and/or function. Identify conserved patterns (motifs) • If one of them has known structure/function, then alignment of other might yield insight about how the structure/functions works. Similar motif ...
The risks and benefits of feeding intact male swine in the United
... to occur at 45 kg, 50 kg is a more practical breaking point when using a two-diet system as in our model. Pigs weighing 20-50 kg For this weight group, average daily gain within a genotype is largely dependent on the animal's daily energy consumption. Genderdifferences do exist, but they are often s ...
... to occur at 45 kg, 50 kg is a more practical breaking point when using a two-diet system as in our model. Pigs weighing 20-50 kg For this weight group, average daily gain within a genotype is largely dependent on the animal's daily energy consumption. Genderdifferences do exist, but they are often s ...
Activated Sugar Precursors: Biosynthetic Pathways and Biological
... compounds in microorganisms, plants and animals. This sugar nucleotide is commonly found in complex carbohydrates that are constituents of the cell wall and of LPS of some Gram-negative bacteria. The presence of L-fucose in these polysaccharides has been shown to play an important role on the intera ...
... compounds in microorganisms, plants and animals. This sugar nucleotide is commonly found in complex carbohydrates that are constituents of the cell wall and of LPS of some Gram-negative bacteria. The presence of L-fucose in these polysaccharides has been shown to play an important role on the intera ...
Herculex® I Product Overview
... In field tests, corn hybrids with Herculex I insect protection averaged 8.3-13.9 bu/acres more than the same hybrids without protection. This means an increased yield of 5 to 9 percent for farmers. With Herculex I insect protection, farmers can use fewer spray pesticides. For years, farmers have use ...
... In field tests, corn hybrids with Herculex I insect protection averaged 8.3-13.9 bu/acres more than the same hybrids without protection. This means an increased yield of 5 to 9 percent for farmers. With Herculex I insect protection, farmers can use fewer spray pesticides. For years, farmers have use ...
PDF
... free amino acids and neurotransmitters contained in organs. Concentration of amino acids and neurotransmitters in rat organs - Free amino acids and neurotransmitters contained in the various rat organs were assayed by the method developed in the present study, and the content levels are shown in Tab ...
... free amino acids and neurotransmitters contained in organs. Concentration of amino acids and neurotransmitters in rat organs - Free amino acids and neurotransmitters contained in the various rat organs were assayed by the method developed in the present study, and the content levels are shown in Tab ...
Participation of DDDD and KPAR
... microbial genomes were estimated to be two to three times the combined plants and animal genomes [8]. These microbes belong to complex communities that reside in different environments such as soil, sea water, air, and human and animals bodies [9]. Due to the fact that these microbes evolved and ada ...
... microbial genomes were estimated to be two to three times the combined plants and animal genomes [8]. These microbes belong to complex communities that reside in different environments such as soil, sea water, air, and human and animals bodies [9]. Due to the fact that these microbes evolved and ada ...
A Quaternion-Based Definition of Protein
... [Table 2. Some structures where overall average straightness is high but labels in the PDB file result in the misappropriation of secondary structure. In this way, straightness can check for errors in PDB files.] ...
... [Table 2. Some structures where overall average straightness is high but labels in the PDB file result in the misappropriation of secondary structure. In this way, straightness can check for errors in PDB files.] ...
Free fatty acids regulate the uncoupling protein and alternative
... Fig. 1. CN-resistant respiration and LA-induced respiration versus LA concentration. Fully depleted FFA rnitochondria (see Section 2) were incubated with 10 mM succinate, 5 Jam rotenone, 2.5 Jag of oligomycin/mg protein, and 0.17 mM ATP (state 4 respiration). CN-resistant respiration (+1 mM KCN) was ...
... Fig. 1. CN-resistant respiration and LA-induced respiration versus LA concentration. Fully depleted FFA rnitochondria (see Section 2) were incubated with 10 mM succinate, 5 Jam rotenone, 2.5 Jag of oligomycin/mg protein, and 0.17 mM ATP (state 4 respiration). CN-resistant respiration (+1 mM KCN) was ...
Acquisition, Conservation, and Loss of Dual
... To date, 72 proteins in Arabidopsis have been shown to be dual targeted (Carrie et al., 2009a; Carrie and Small, 2013); however, as many as 500 are predicted to be dual targeted because they contain ambiguous signals (Mitschke et al., 2009). The necessity for dual targeting remains largely unknown, ...
... To date, 72 proteins in Arabidopsis have been shown to be dual targeted (Carrie et al., 2009a; Carrie and Small, 2013); however, as many as 500 are predicted to be dual targeted because they contain ambiguous signals (Mitschke et al., 2009). The necessity for dual targeting remains largely unknown, ...
Zaenab Aljassim thesis-6_1
... I, Zaenab Al Jassim, hereby grant to Laurentian University and/or its agents the non-exclusive license to archive and make accessible my thesis, dissertation, or project report in whole or in part in all forms of media, now or for the duration of my copyright ownership. I retain all other ownership ...
... I, Zaenab Al Jassim, hereby grant to Laurentian University and/or its agents the non-exclusive license to archive and make accessible my thesis, dissertation, or project report in whole or in part in all forms of media, now or for the duration of my copyright ownership. I retain all other ownership ...
Proteins and their Ligands: Their Importance and how to Crystallize
... substrate or ligand is bound, second, a protein with two or more bound substrates and finally, the structures of proteins, in which the product of the reaction cycle is present in the active site. Within this chapter the conformational changes induced by ligand(s) of three different protein families ...
... substrate or ligand is bound, second, a protein with two or more bound substrates and finally, the structures of proteins, in which the product of the reaction cycle is present in the active site. Within this chapter the conformational changes induced by ligand(s) of three different protein families ...
Studier`s autoinduction media
... To label target proteins with Se-Met, PASM-5052 auto-inducing medium is inoculated with a fresh overnight culture grown in PA-0.5G. Growth at 37C from a thousand-fold dilution into PASM5052 typically reaches saturation in 14-16 hours. Growth at 20C is much slower and a culture can take 3 days or l ...
... To label target proteins with Se-Met, PASM-5052 auto-inducing medium is inoculated with a fresh overnight culture grown in PA-0.5G. Growth at 37C from a thousand-fold dilution into PASM5052 typically reaches saturation in 14-16 hours. Growth at 20C is much slower and a culture can take 3 days or l ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... catalyzes the conversion of myo-inositol 1,3,4,5,6-pentakisphosphate to phytic acid. In addition, it is also capable of catalyzing the phosphorylation of myo-inositol 1,4,6-trisphosphate, myo-inositol 1,4,5,6-tetrakisphosphate, and myo-inositol 3,4,5,6tetrakisphosphate. Nuclear magnetic resonance sp ...
... catalyzes the conversion of myo-inositol 1,3,4,5,6-pentakisphosphate to phytic acid. In addition, it is also capable of catalyzing the phosphorylation of myo-inositol 1,4,6-trisphosphate, myo-inositol 1,4,5,6-tetrakisphosphate, and myo-inositol 3,4,5,6tetrakisphosphate. Nuclear magnetic resonance sp ...
Effects of Macromolecular Crowding on Protein Folding
... kDa). A theoretical crowding model was used to investigate the origins of this stabilization. In this model, Dextran and Ficoll were modeled as elongated rods and the protein was represented as a sphere, with the folded sphere representation being smaller than the unfolded sphere representation. Not ...
... kDa). A theoretical crowding model was used to investigate the origins of this stabilization. In this model, Dextran and Ficoll were modeled as elongated rods and the protein was represented as a sphere, with the folded sphere representation being smaller than the unfolded sphere representation. Not ...
The Effect of Detergents on Amino Acid Liberation by
... the parent strain were mainly short-chain neutral types (glycine, alanine, serine and threonine) and ornithine and methionine. It is possible that the uptake systems which mediate the transport of these amino acids were specifically affected by the detergent. Differences between the effects of the t ...
... the parent strain were mainly short-chain neutral types (glycine, alanine, serine and threonine) and ornithine and methionine. It is possible that the uptake systems which mediate the transport of these amino acids were specifically affected by the detergent. Differences between the effects of the t ...
Responses of cows to abomasal infusion of lysine and methionine at
... both diets. A l l feedstuffs were incubated in the rumen using a standard in situ method described below. The in situ effective degradability values were used in linear programming software (Gavish, Giva't Brenner 60948, Israel) to compose the two diets. Complementary ingredients were calculated usi ...
... both diets. A l l feedstuffs were incubated in the rumen using a standard in situ method described below. The in situ effective degradability values were used in linear programming software (Gavish, Giva't Brenner 60948, Israel) to compose the two diets. Complementary ingredients were calculated usi ...
Crystal Structures of the Oxidized and Reduced Forms of UDP
... either side by R-helices. The C-terminal domain contains three strands of β-pleated sheet, two major R-helices, and one helical turn. In addition to allowing for a threedimensional structure of the enzyme to be modeled, this X-ray crystallographic investigation proved that the protein binds two NAD+ ...
... either side by R-helices. The C-terminal domain contains three strands of β-pleated sheet, two major R-helices, and one helical turn. In addition to allowing for a threedimensional structure of the enzyme to be modeled, this X-ray crystallographic investigation proved that the protein binds two NAD+ ...
Tricarboxylic Acid Cycle (TCA), Krebs Cycle
... The inner mitochondrial membrane is impermeable to the most charged and hydrophilic substances. However it contains numerous transport proteins that permit the passage of specific molecules. 1- ATP-ADP transport, see oxid-phospho, Transporter for ADP & Pi from cytosol into mitochondria by specialize ...
... The inner mitochondrial membrane is impermeable to the most charged and hydrophilic substances. However it contains numerous transport proteins that permit the passage of specific molecules. 1- ATP-ADP transport, see oxid-phospho, Transporter for ADP & Pi from cytosol into mitochondria by specialize ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.