Chemical synthesis of proteins
... is limited by the synthetic efficiency of each step. As discussed by Kent22, the synthesis of a 100-residue protein with 99.9% efficiency at each step provides a 90% overall yield of the desired product; 97% efficiency provides a 5% overall yield. The effective solvation of the peptide–resin complex ...
... is limited by the synthetic efficiency of each step. As discussed by Kent22, the synthesis of a 100-residue protein with 99.9% efficiency at each step provides a 90% overall yield of the desired product; 97% efficiency provides a 5% overall yield. The effective solvation of the peptide–resin complex ...
... for the binding of the ligand and the reaction that is to be catalyzed. By studying the three-dimensional structure of a protein, it may be possible to identify possible binding sites through features that often are common for these. An active site may also be identified by introducing mutations in ...
Active Site Interactions in Oligomeric Structures of Inorganic
... amino acids. This method replaced chemical modifications, whose weak points were evident. Even a highly specific chemical reagent used for modification is an additional component introduced into the enzyme molecule and therefore may play a pivotal role in changing properties of the protein. However, ...
... amino acids. This method replaced chemical modifications, whose weak points were evident. Even a highly specific chemical reagent used for modification is an additional component introduced into the enzyme molecule and therefore may play a pivotal role in changing properties of the protein. However, ...
Milk Components - Great Lakes Regional Dairy Conference
... October 6 to January 6 for fat and November 21 to December 12 for protein • Amplitude of the rhythm ranged from 0.07 to 0.14% for fat and from 0.08 to 0.12% for protein • Amplitude for fat from generally lower in southern and higher in northern • Range in fat and protein in Mideast was 0.26% for fat ...
... October 6 to January 6 for fat and November 21 to December 12 for protein • Amplitude of the rhythm ranged from 0.07 to 0.14% for fat and from 0.08 to 0.12% for protein • Amplitude for fat from generally lower in southern and higher in northern • Range in fat and protein in Mideast was 0.26% for fat ...
NIH Public Access
... [1-5]. Two S6K1 isoforms (Accession No. NM003161; αI and αII isoforms) are produced from a single gene by alternative mRNA splicing and the use of an alternative translational start site [6]. The 525 residue αI isoform contains an N-terminal 23 residue segment that encodes a polybasic nuclear locali ...
... [1-5]. Two S6K1 isoforms (Accession No. NM003161; αI and αII isoforms) are produced from a single gene by alternative mRNA splicing and the use of an alternative translational start site [6]. The 525 residue αI isoform contains an N-terminal 23 residue segment that encodes a polybasic nuclear locali ...
Nutritional Neurotransmitter Balancing Protocols
... regulatory function, synaptic neurotransmitter levels of the remaining viable neurons must be increased to levels higher than is normally found in the system, which restores adequate electrical outflow resulting in relief of symptoms and optimal regulatory function.” (Hinz et al 2009) ...
... regulatory function, synaptic neurotransmitter levels of the remaining viable neurons must be increased to levels higher than is normally found in the system, which restores adequate electrical outflow resulting in relief of symptoms and optimal regulatory function.” (Hinz et al 2009) ...
histidine and cysteine can enhance the metabolic reaction rates in
... one order of magnitude than that of histidine, which showed a specific relationship between an amino acid and an anticodon, namely the stereochemical theory of the genetic code. I have also shown in a separate letter [3] that not only histidine but other single amino acids such as cysteine, glutamic ...
... one order of magnitude than that of histidine, which showed a specific relationship between an amino acid and an anticodon, namely the stereochemical theory of the genetic code. I have also shown in a separate letter [3] that not only histidine but other single amino acids such as cysteine, glutamic ...
EMBO EMBO EMBO
... (638NAGII O4yGlu276 Oe2, 3.38 Å, 638ManI O2yGlu276 Oe2, 2.02 Å) and to strands b1 and b9 at the domain I–II interface (638ManII O3yArg354 NZ1, 3.46 Å; 638ManI O2yArg354 NZ1, 3.25 Å). This interaction is one of the few structurally well-defined cases of a protein–carbohydrate contact involved in ...
... (638NAGII O4yGlu276 Oe2, 3.38 Å, 638ManI O2yGlu276 Oe2, 2.02 Å) and to strands b1 and b9 at the domain I–II interface (638ManII O3yArg354 NZ1, 3.46 Å; 638ManI O2yArg354 NZ1, 3.25 Å). This interaction is one of the few structurally well-defined cases of a protein–carbohydrate contact involved in ...
Reasons for the occurrence of the twenty coded protein amino acids
... amino acid with an a-hydrogen or by the carboxylation of an amine such as isopropylamine. Replacement of the a-hydrogen by larger substituent, such as a methyl group, would also increase significantly steric hinderance around the amino and carboxyl groups. Steric difficulties have been encountered i ...
... amino acid with an a-hydrogen or by the carboxylation of an amine such as isopropylamine. Replacement of the a-hydrogen by larger substituent, such as a methyl group, would also increase significantly steric hinderance around the amino and carboxyl groups. Steric difficulties have been encountered i ...
Document
... metabolism, as well as our lack of knowledge of the way new genes are created, selecting function first, then recruiting a structure that will be improved as it is submitted to natural selection for increased fitness of its host (acquisitive evolution) ...
... metabolism, as well as our lack of knowledge of the way new genes are created, selecting function first, then recruiting a structure that will be improved as it is submitted to natural selection for increased fitness of its host (acquisitive evolution) ...
Importance of main-chain hydrophobic free energy to the stability of
... spectrum, containing proteins from moderately thermophilic organisms and their mesophilic homologues. Here we select one pair from each family. The three-dimensional structures of all these proteins have been taken from Protein Data Bank [28]. For a given protein, the PDB files contain coordinates f ...
... spectrum, containing proteins from moderately thermophilic organisms and their mesophilic homologues. Here we select one pair from each family. The three-dimensional structures of all these proteins have been taken from Protein Data Bank [28]. For a given protein, the PDB files contain coordinates f ...
A Major Surface Protein on Group A Streptococci Is a
... in the colonization of mucosal surfaces by microorganisms (5, 26). However, the soluble form of fibronectin found in saliva (17, 27) may also play a key role in the colonization process, since this form would be the first encountered by the incoming bacterium and may serve as a competitive inhibitor ...
... in the colonization of mucosal surfaces by microorganisms (5, 26). However, the soluble form of fibronectin found in saliva (17, 27) may also play a key role in the colonization process, since this form would be the first encountered by the incoming bacterium and may serve as a competitive inhibitor ...
Protein for Athletes
... is the largest storage site for amino acids.1 However, muscle is more than just protein; it also contains water, fat, glycogen, and some minerals. One pound of muscle contains 70 to 105 g of protein, and to build a pound of muscle, it is estimated that 10 to 14 g of additional protein is needed each ...
... is the largest storage site for amino acids.1 However, muscle is more than just protein; it also contains water, fat, glycogen, and some minerals. One pound of muscle contains 70 to 105 g of protein, and to build a pound of muscle, it is estimated that 10 to 14 g of additional protein is needed each ...
2.7. Future of plant-based protein sources
... Usage of the product, how it is used and what for. These are issues that are important especially when the product is not very familiar already. Ease of use is important to many participants, and when a product is easy to use, it should be made very clear. Substituting meat should not be emphasized ...
... Usage of the product, how it is used and what for. These are issues that are important especially when the product is not very familiar already. Ease of use is important to many participants, and when a product is easy to use, it should be made very clear. Substituting meat should not be emphasized ...
TAR-RNA binding by HIV-1 Tat protein is
... substrate, introduced the idea of enzyme flexibility, whereby the mutual fitting of the interacting partners is described by the ‘hand-in-glove’ metaphor (30). (ii) The idea that natural nucleic acids have generally higher affinities for L- than for D-proteins had already been experimentally disprov ...
... substrate, introduced the idea of enzyme flexibility, whereby the mutual fitting of the interacting partners is described by the ‘hand-in-glove’ metaphor (30). (ii) The idea that natural nucleic acids have generally higher affinities for L- than for D-proteins had already been experimentally disprov ...
isis/DraW - Accelrys
... ISIS/DRAW ISIS/Draw is a chemically intelligent drawing program that understands the fundamentals of chemistry such as valence limits, bond angles, and aromatic ring systems. This understanding enables users to create chemical graphics intuitively, which can be inserted into documents or used to bui ...
... ISIS/DRAW ISIS/Draw is a chemically intelligent drawing program that understands the fundamentals of chemistry such as valence limits, bond angles, and aromatic ring systems. This understanding enables users to create chemical graphics intuitively, which can be inserted into documents or used to bui ...
X(0,25) - IFSC-USP
... Sources of Errors in Gene Notation • Incorrect assembly of DNA due to insufficient overlap redundancy, common in extremely high or low GC regions. • Wrong strand choice and wrong frame choice caused by programmed maximization of gene length. Especially challenging in high GC genomes having Multiply ...
... Sources of Errors in Gene Notation • Incorrect assembly of DNA due to insufficient overlap redundancy, common in extremely high or low GC regions. • Wrong strand choice and wrong frame choice caused by programmed maximization of gene length. Especially challenging in high GC genomes having Multiply ...
Intrinsically Disordered Proteins as Drug Targets
... Drug design is a long complex process. Translating basic research to the market (passing through drug discovery, preclinical and clinical studies) takes tens of years and costs billions of dollars. Computational techniques help to shorten the process economizing it. During the last three decades Com ...
... Drug design is a long complex process. Translating basic research to the market (passing through drug discovery, preclinical and clinical studies) takes tens of years and costs billions of dollars. Computational techniques help to shorten the process economizing it. During the last three decades Com ...
Gene Section BRD4 (bromodomain containing 4) Atlas of Genetics and Cytogenetics
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
... The BRD4-NUT fusion protein is composed of the Nterminal of BRD4 (amino acids 1-720 out of 1372) and almost the entire protein sequence of NUT (amino acids 6-1127). The N-terminal of BRD4 includes bromodomains 1 and 2 and other, less well ...
Structure of the enzyme-acyl carrier protein (ACP) substrate
... biotin protein ligase (5, 6), which is in contrast to other bacteria where bioH resides within the biotin operon (7). Thus, the E. coli bioH gene may encode a less-specific protein than those encoded by the integrated bioH genes. In this paper we report experiments indicating that BioH acts before Bi ...
... biotin protein ligase (5, 6), which is in contrast to other bacteria where bioH resides within the biotin operon (7). Thus, the E. coli bioH gene may encode a less-specific protein than those encoded by the integrated bioH genes. In this paper we report experiments indicating that BioH acts before Bi ...
Gene Section MAP2 (microtubule associated protein 2) -
... protein to bind to the microtubules. A 31 amino acid long repeating motif is characteristic of this protein. However, it is found that this motif is not sufficient by itself to bind to microtubules. Two contiguous sequences on either end of this repeating structure on both the amino and carboxyl end ...
... protein to bind to the microtubules. A 31 amino acid long repeating motif is characteristic of this protein. However, it is found that this motif is not sufficient by itself to bind to microtubules. Two contiguous sequences on either end of this repeating structure on both the amino and carboxyl end ...
Limited Proteolysis
... Figure 2. The MALDI-TOF spectra of (a) undigested, (b) semi-digested for 15 minutes with 0.5 mL immobilized trypsin, and (c) fully-digested for 24hours with 0.5 mL immobilized trypsin MDH. A spectrum of the undigested protein is seen in Figure 2a and shows only the standards used (myoglobin ~17 kDa, ...
... Figure 2. The MALDI-TOF spectra of (a) undigested, (b) semi-digested for 15 minutes with 0.5 mL immobilized trypsin, and (c) fully-digested for 24hours with 0.5 mL immobilized trypsin MDH. A spectrum of the undigested protein is seen in Figure 2a and shows only the standards used (myoglobin ~17 kDa, ...
Leukaemia Section t(5;9)(q33;q22) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Centromere to telomere orientation; exons: 13-14 (alternative spliced). Protein SYK is a nonreceptor protein kinase that serves as a key regulator of multiple biochemical signal transduction events and has high homology to ZAP70 protein tyrosine kinase. In contrast to ITK, a translocation of SYK has ...
... Centromere to telomere orientation; exons: 13-14 (alternative spliced). Protein SYK is a nonreceptor protein kinase that serves as a key regulator of multiple biochemical signal transduction events and has high homology to ZAP70 protein tyrosine kinase. In contrast to ITK, a translocation of SYK has ...
Protein structure prediction
Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.