
Carbohydrates and Glycobiology
... photosynthesis converts more than 100 billion metric tons of CO2 and H2O into cellulose and other plant products. Certain carbohydrates (sugar and starch) are a dietary staple in most parts of the world, and the oxidation of carbohydrates is the central energy-yielding pathway in most nonphotosynthe ...
... photosynthesis converts more than 100 billion metric tons of CO2 and H2O into cellulose and other plant products. Certain carbohydrates (sugar and starch) are a dietary staple in most parts of the world, and the oxidation of carbohydrates is the central energy-yielding pathway in most nonphotosynthe ...
Structural characterization of an antibody in complex with
... The PSA:Asp98 side chain can form hydrogen bonds with antibody residue H:Thr28 or H:Thr31. At one edge of the binding interface the PSA loop residue PSA:Lys175 also forms a hydrogen bond with antibody residue H:Thr28. Another PSA lysine residue PSA:Lys178 is within hydrogen bond distance with antibo ...
... The PSA:Asp98 side chain can form hydrogen bonds with antibody residue H:Thr28 or H:Thr31. At one edge of the binding interface the PSA loop residue PSA:Lys175 also forms a hydrogen bond with antibody residue H:Thr28. Another PSA lysine residue PSA:Lys178 is within hydrogen bond distance with antibo ...
Arabidopsis Genes Encoding Components of the
... similarity is that these short open reading frames are examples of the evolutionary process of exon shuffling in progress. In the case of AtToc75-IV, the region of similarity extends for approximately 50% of the length of the larger Toc75 homologs. It is possible that this may be enough for the prot ...
... similarity is that these short open reading frames are examples of the evolutionary process of exon shuffling in progress. In the case of AtToc75-IV, the region of similarity extends for approximately 50% of the length of the larger Toc75 homologs. It is possible that this may be enough for the prot ...
- Sportscience
... purported anabolic amino acids, branched-chain amino acids, glutamine, creatine, and hydroxymethylbutyrate (HMB). LITERATURE. Two books, 61 research articles, 10 published abstracts, and 19 review articles or book chapters. FINDINGS. Dietary supplementation of protein beyond that necessary to mainta ...
... purported anabolic amino acids, branched-chain amino acids, glutamine, creatine, and hydroxymethylbutyrate (HMB). LITERATURE. Two books, 61 research articles, 10 published abstracts, and 19 review articles or book chapters. FINDINGS. Dietary supplementation of protein beyond that necessary to mainta ...
Development of a novel analytical approach combining the quantification of
... In that respect, metabolomics is the science of small molecules which are involved in biochemical processes and it has been shown that this approach can provide a great deal of information regarding the ‘‘real-time’’ situation of a biological system within a biochemical approach.21 Metabolomics is t ...
... In that respect, metabolomics is the science of small molecules which are involved in biochemical processes and it has been shown that this approach can provide a great deal of information regarding the ‘‘real-time’’ situation of a biological system within a biochemical approach.21 Metabolomics is t ...
BIOCHEMISTRY LAB CHE-554 First portion: A chromophorogenic
... Non-absorbing compounds can be detected via a reaction that generates a chromophore in proportion to the compound’s concentration. ! Either a known ! or a standard curve are used to relate the A to the starting compound’s concentration. (The standard curve in-essence yields a !). ! We will use the B ...
... Non-absorbing compounds can be detected via a reaction that generates a chromophore in proportion to the compound’s concentration. ! Either a known ! or a standard curve are used to relate the A to the starting compound’s concentration. (The standard curve in-essence yields a !). ! We will use the B ...
Preference for and learning of amino acids in larval
... Our data suggest that the profile of amino acid preference changes across larval instars and that starvation during the third instar also alters this profile (Fig. 1). The fact that these modulations how amino acids are sensed and/or processed (also see Introduction). These modulations further imply ...
... Our data suggest that the profile of amino acid preference changes across larval instars and that starvation during the third instar also alters this profile (Fig. 1). The fact that these modulations how amino acids are sensed and/or processed (also see Introduction). These modulations further imply ...
Inhibition of Protein Kinases by Balanol: Specificity within the Serine
... experimental condition was duplicated within each assay, and each assay was replicated two to five times, as noted. Protein content was estimated by the method of Bradford (1976) using BSA as a standard. Activities of PKA and PKC were assessed as previously described (Koide et al., 1995). PKG activi ...
... experimental condition was duplicated within each assay, and each assay was replicated two to five times, as noted. Protein content was estimated by the method of Bradford (1976) using BSA as a standard. Activities of PKA and PKC were assessed as previously described (Koide et al., 1995). PKG activi ...
Capturing protein interactions in the secretory pathway of living cells
... membrane, and nuclear protein–protein and protein–RNA interactions (13–16). In the present study, we have explored the suitability of the YFP-based PCA technique for visualizing protein–protein interactions in the lumen of the early secretory pathway by using ERGIC-53 as model protein. ERGIC-53 is a ...
... membrane, and nuclear protein–protein and protein–RNA interactions (13–16). In the present study, we have explored the suitability of the YFP-based PCA technique for visualizing protein–protein interactions in the lumen of the early secretory pathway by using ERGIC-53 as model protein. ERGIC-53 is a ...
S2 Protocol.
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
... calculate the similarity score (S) which is converted to distance d. The source code for calculating Bioisosteric similarity score was obtained from Dr. Michael Hutter (personal communication). We cannot share the source code due to license restrictions. 4) SMILIGN is a new method we developed as pa ...
Access of the substrate to the active site of squalene and
... SHC has long been the only template structure for building homology models that is able to give important mechanistic insights into oxidosqualene cyclization by eukaryotic OSCs [12]. It was obvious, however, that SHC was only of limited use as a model for unravelling the OSC reaction, because of dif ...
... SHC has long been the only template structure for building homology models that is able to give important mechanistic insights into oxidosqualene cyclization by eukaryotic OSCs [12]. It was obvious, however, that SHC was only of limited use as a model for unravelling the OSC reaction, because of dif ...
Global and Local Sequence Alignment
... Multiplying all substitution and gap scores by a positive constant does not change the optimal alignment. Why? Adding a constant < to all substitution scores, and 2 to all gap scores, does not change the optimal alignment. Why? A global alignment scoring system with the three nominal parameters of ...
... Multiplying all substitution and gap scores by a positive constant does not change the optimal alignment. Why? Adding a constant < to all substitution scores, and 2 to all gap scores, does not change the optimal alignment. Why? A global alignment scoring system with the three nominal parameters of ...
Origin and evolution of peptide-modifying
... Figure 1. Predicted hydroxy/hydroperoxy wybutosine biosynthesis pathway and domain architecture of key enzymes involved in its synthesis. (A) Shown above each enzyme of the reaction pathway, in colored squares, is the phyletic profile of the protein family. The color intensity of the outline is used ...
... Figure 1. Predicted hydroxy/hydroperoxy wybutosine biosynthesis pathway and domain architecture of key enzymes involved in its synthesis. (A) Shown above each enzyme of the reaction pathway, in colored squares, is the phyletic profile of the protein family. The color intensity of the outline is used ...
Can the Origin of the Genetic Code Be Explained - BIO
... The actual physical mediators of the code, however, are transfer RNAs (tRNAs) that, after being charged with their specific amino acids by enzymes known as aminoacyl transfer RNA synthetases (aaRSs), present the amino acids for peptide bond formation in the peptidyl-transferase (P) site of the ribos ...
... The actual physical mediators of the code, however, are transfer RNAs (tRNAs) that, after being charged with their specific amino acids by enzymes known as aminoacyl transfer RNA synthetases (aaRSs), present the amino acids for peptide bond formation in the peptidyl-transferase (P) site of the ribos ...
prosite.excerpt
... asparagine residue is glycosylated, due to the fact that the folding of the protein plays an important role in the regulation of N-glycosylation [2]. It has been shown [3] that the presence of proline between Asn and Ser/Thr will inhibit N-glycosylation; this has been confirmed by a recent [4] stati ...
... asparagine residue is glycosylated, due to the fact that the folding of the protein plays an important role in the regulation of N-glycosylation [2]. It has been shown [3] that the presence of proline between Asn and Ser/Thr will inhibit N-glycosylation; this has been confirmed by a recent [4] stati ...
Characterisation of the Aspergillus niger dapB gene, which encodes
... in G. zeae and N. crassa lack the intron at the start of the gene which is present in the other genes that encode extracellular DPP IVs. The protein sequences, 28 in total, were deduced from the DNA sequences, or retrieved from protein databases, and subsequently analysed for the presence of putativ ...
... in G. zeae and N. crassa lack the intron at the start of the gene which is present in the other genes that encode extracellular DPP IVs. The protein sequences, 28 in total, were deduced from the DNA sequences, or retrieved from protein databases, and subsequently analysed for the presence of putativ ...
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in
... Another MALDI-based approach that provides amino acid sequence information on peptides relies on the fragmentation of the ions in the field-free drift region (between the ion source and the detector) of the TOF mass analyzer. This fragmentation, referred to as PSD, is due to collisions between analy ...
... Another MALDI-based approach that provides amino acid sequence information on peptides relies on the fragmentation of the ions in the field-free drift region (between the ion source and the detector) of the TOF mass analyzer. This fragmentation, referred to as PSD, is due to collisions between analy ...
Creation/Evolution - Geoscience Research Institute
... representing a few amino acids or possibly one codon representing a “group” of amino acids More precise codon meaning evolves perhaps with only the first two bases having meaning with discrimination at the third position evolving later The code becomes “frozen” when the system becomes so complex tha ...
... representing a few amino acids or possibly one codon representing a “group” of amino acids More precise codon meaning evolves perhaps with only the first two bases having meaning with discrimination at the third position evolving later The code becomes “frozen” when the system becomes so complex tha ...
N-Terminal Intramolecularly Conserved Histidines of Three Domains
... N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nucleotide level these are 75-80% identical in sequence ove ...
... N-terminal sequence of 111 amino acids, which is homologous to the N-terminal region of the substrate-binding protein, LBP. This is followed by three contiguous domains, designated D1, D2, and D3, which are homologous to one another. At the nucleotide level these are 75-80% identical in sequence ove ...
Protein structure prediction

Protein structure prediction is the prediction of the three-dimensional structure of a protein from its amino acid sequence — that is, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry; it is highly important in medicine (for example, in drug design) and biotechnology (for example, in the design of novel enzymes). Every two years, the performance of current methods is assessed in the CASP experiment (Critical Assessment of Techniques for Protein Structure Prediction). A continuous evaluation of protein structure prediction web servers is performed by the community project CAMEO3D.