Complete genome sequence of the thermophilic Thermus sp
... been suggested to play an essential role in plasmid replication and partition [36]. In addition, a putative integrase gene that facilitates gene transfer and chromosome modification can be found in both plasmids. ...
... been suggested to play an essential role in plasmid replication and partition [36]. In addition, a putative integrase gene that facilitates gene transfer and chromosome modification can be found in both plasmids. ...
ARTICLES - Weizmann Institute of Science
... Eukaryotic genomic DNA exists as highly compacted nucleosome arrays called chromatin. Each nucleosome contains a 147-base-pair (bp) stretch of DNA, which is sharply bent and tightly wrapped around a histone protein octamer1. This sharp bending occurs at every DNA helical repeat (,10 bp), when the ma ...
... Eukaryotic genomic DNA exists as highly compacted nucleosome arrays called chromatin. Each nucleosome contains a 147-base-pair (bp) stretch of DNA, which is sharply bent and tightly wrapped around a histone protein octamer1. This sharp bending occurs at every DNA helical repeat (,10 bp), when the ma ...
Codon usage bias from tRNA`s point of view
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
Telomere maintenance without telomerase
... Dierent telomeres in post-senescent survivors exhibited variable lengths, often with tract lengths longer than telomeres of a telomerase-pro®cient strain. However, these elongated telomeres still exhibited the same gradual shortening observed during the initial senescence of a newly generated telom ...
... Dierent telomeres in post-senescent survivors exhibited variable lengths, often with tract lengths longer than telomeres of a telomerase-pro®cient strain. However, these elongated telomeres still exhibited the same gradual shortening observed during the initial senescence of a newly generated telom ...
Mechanisms of Transcription-Replication
... the elongation rate for RNA polymerase is just 50 nucleotides (nt) per s (15); i.e., replication is approximately 20-fold faster than transcription. Since the two processes proceed simultaneously, frequent collisions between replication and transcription seem unavoidable (3, 38). Given that both pro ...
... the elongation rate for RNA polymerase is just 50 nucleotides (nt) per s (15); i.e., replication is approximately 20-fold faster than transcription. Since the two processes proceed simultaneously, frequent collisions between replication and transcription seem unavoidable (3, 38). Given that both pro ...
Investigating the DNA-Binding Interactions of Small
... Cancer is one the leading causes of death in the United States. Researchers have focused on designing and developing therapeutics aimed at DNA in order to treat cancer and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a ...
... Cancer is one the leading causes of death in the United States. Researchers have focused on designing and developing therapeutics aimed at DNA in order to treat cancer and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a ...
icsi sperm selection by ha binding
... microscopic assay for the assessment of the proportion of spermatozoa that would bind to the zona pellucida. The frequencies of chromosomal disomies and diploidies are decreased within the normal range of conventional IVF. Mature spermatozoa selected by virtue of HA-binding are also viable, and ...
... microscopic assay for the assessment of the proportion of spermatozoa that would bind to the zona pellucida. The frequencies of chromosomal disomies and diploidies are decreased within the normal range of conventional IVF. Mature spermatozoa selected by virtue of HA-binding are also viable, and ...
as a PDF - CiteSeerX
... replication by the LIR, leading to recombinationally active initiating structures. Such interactions predict replication-related, MMR-independent genome changes. tions in higher organisms are proposed to interact with mismatches in some way to inhibit homeologous recombination, possibly by preventin ...
... replication by the LIR, leading to recombinationally active initiating structures. Such interactions predict replication-related, MMR-independent genome changes. tions in higher organisms are proposed to interact with mismatches in some way to inhibit homeologous recombination, possibly by preventin ...
Exceptionally high levels of recombination
... The first draft of the honey bee genome sequence and improved genetic maps are utilized to analyze a genome displaying 10 times higher levels of recombination (19 cM/Mb) than previously analyzed genomes of higher eukaryotes. The exceptionally high recombination rate is distributed genome-wide, but v ...
... The first draft of the honey bee genome sequence and improved genetic maps are utilized to analyze a genome displaying 10 times higher levels of recombination (19 cM/Mb) than previously analyzed genomes of higher eukaryotes. The exceptionally high recombination rate is distributed genome-wide, but v ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
... of Hoechst here was in ~ 1.5 - 2 times less than in the case of HP1 or t-RNA. It means that Hoechst interacts with the double helix not such strong as with single chains or hairpin structures. The life-time of Hoechst at binding with DNA was increased from 0.4 ns to only 3 ns. Furthermore, there was ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... Phylogenetic analyses. All of the sequences determined in this work were compared with group I introns downloaded from GenBank [http:// www.ncbi.nlm.nih.gov]. Sequences were aligned using the default parameters implemented in Muscle 3.6 [13] and then adjusted with BioEdit 7.0 [20]. For constructing ...
... Phylogenetic analyses. All of the sequences determined in this work were compared with group I introns downloaded from GenBank [http:// www.ncbi.nlm.nih.gov]. Sequences were aligned using the default parameters implemented in Muscle 3.6 [13] and then adjusted with BioEdit 7.0 [20]. For constructing ...
Lesson Overview - Enfield High School
... Some of the variation produced by mutations can be highly advantageous to an organism or species. Mutations often produce proteins with new or altered functions that can be useful to organisms in different or changing environments. For example, mutations have helped many insects resist chemical pe ...
... Some of the variation produced by mutations can be highly advantageous to an organism or species. Mutations often produce proteins with new or altered functions that can be useful to organisms in different or changing environments. For example, mutations have helped many insects resist chemical pe ...
2. Assessing the probative value of DNA evidence
... notorious recent miscarriages of justice involving statistical evidence have exposed errors by experts. ...
... notorious recent miscarriages of justice involving statistical evidence have exposed errors by experts. ...
Assessing the Probative Value of DNA Evidence
... notorious recent miscarriages of justice involving statistical evidence have exposed errors by experts. ...
... notorious recent miscarriages of justice involving statistical evidence have exposed errors by experts. ...
Localization and structural analysis of the ribosomal RNA operons of
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
... E. coli rRNA operons. The fact that insertion of a cartridge resulted in the loss of the wild type signal, leads to the conclusion that there are only three rRNA operons in R. sphaeroides. Thus rmA can be assigned to the 10 kb BamYU signal, rrnB to the 14 kb BamYU signal, and rmC to the 13 kb BamYU ...
Surgical Sperm Retrieval
... If sperm is not found in the fluid taken from the epididymis via either the MESA or PESA procedure a small sample of testicular tissue can be taken. This is then examined back at the laboratory, sperm can be extracted and either frozen or used fresh for ICSI. There is usually a little bruising and t ...
... If sperm is not found in the fluid taken from the epididymis via either the MESA or PESA procedure a small sample of testicular tissue can be taken. This is then examined back at the laboratory, sperm can be extracted and either frozen or used fresh for ICSI. There is usually a little bruising and t ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
... the plasmid do not share homology with the yeast genome, circular inverted (head-to-head) dimer plasmids are theprincipal product of repair. By measurements of the DNA concentration dependence of transformation with alinearized plasmid, and by transformation with mixtures of genetically marked plasm ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
... SCCs3 in the aerodigestive tract are typically chemical carcinogen induced human malignancies closely associated with tobacco expo sure and alcohol consumption (1, 2). To define the molecular events involved in oral squamous mucosa neoplastic transformation, we have established a murine model which ...
... SCCs3 in the aerodigestive tract are typically chemical carcinogen induced human malignancies closely associated with tobacco expo sure and alcohol consumption (1, 2). To define the molecular events involved in oral squamous mucosa neoplastic transformation, we have established a murine model which ...
Charge Transport in DNA - Insights from
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
Analysis of clones carrying repeated DNA sequences in two YAC
... clones in adjacent well positions and are likely to be duplicate clones caused by spill-over from the neighbouring well. Southern blot analysis of YACs separated by pulsed field gel electrophoresis established that 26 (17%) of these 155 clones are chimaeric between chloroplast DNA and single-copy nu ...
... clones in adjacent well positions and are likely to be duplicate clones caused by spill-over from the neighbouring well. Southern blot analysis of YACs separated by pulsed field gel electrophoresis established that 26 (17%) of these 155 clones are chimaeric between chloroplast DNA and single-copy nu ...
Horizontal transfer generates genetic variation in an asexual
... homologous elements than the core genome. In addition, we discovered, in LS regions of JR2, several structural forms of nonhomologous recombination, and two or three homologous sequence types of each form, with almost each sequence type present in an LS region of another strain. A large section of o ...
... homologous elements than the core genome. In addition, we discovered, in LS regions of JR2, several structural forms of nonhomologous recombination, and two or three homologous sequence types of each form, with almost each sequence type present in an LS region of another strain. A large section of o ...
Isolation, Characterization, and Annotation: The Search for Novel
... a gene annotation tool, was then used to analyze potential genes. The research class divided the genomes into 5-6 kbp segments to be analyzed by the students in pairs. Each segment was annotated by multiple groups. Using Apollo, annotations were added to the genomes with length, start codon, gap/ove ...
... a gene annotation tool, was then used to analyze potential genes. The research class divided the genomes into 5-6 kbp segments to be analyzed by the students in pairs. Each segment was annotated by multiple groups. Using Apollo, annotations were added to the genomes with length, start codon, gap/ove ...
Rates of Spontaneous Mutation
... lytic RNA viruses, a retrovirus or retrotransposon chromosome replicates precisely three times per infective cycle. Transcription by the host RNA polymerase produces an RNA genome. Reverse transcriptase then catalyzes two replications to generate a DNA-based chromosome that integrates into the host ...
... lytic RNA viruses, a retrovirus or retrotransposon chromosome replicates precisely three times per infective cycle. Transcription by the host RNA polymerase produces an RNA genome. Reverse transcriptase then catalyzes two replications to generate a DNA-based chromosome that integrates into the host ...
Application Note: Targeted sequencing and chromosomal haplotype
... complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application o ...
... complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application o ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... average genome-wide heritability of DNA methylation was higher when restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher a ...
... average genome-wide heritability of DNA methylation was higher when restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher a ...
Mitochondrial DNA
Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus and, in plants, in the chloroplast.In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs. Human mitochondrial DNA was the first significant part of the human genome to be sequenced. In most species, including humans, mtDNA is inherited solely from the mother.The DNA sequence of mtDNA has been determined from a large number of organisms and individuals (including some organisms that are extinct), and the comparison of those DNA sequences represents a mainstay of phylogenetics, in that it allows biologists to elucidate the evolutionary relationships among species. It also permits an examination of the relatedness of populations, and so has become important in anthropology and field biology.