Pathogen induced genome instability
... Many bacteria have surface proteins that recognize and import naked DNA from closely related bacterial species Lacking such proteins, E. coli can be artificially induced to take up foreign DNA by incubating them in a culture medium that has a high concentration of calcium ions. This technique is use ...
... Many bacteria have surface proteins that recognize and import naked DNA from closely related bacterial species Lacking such proteins, E. coli can be artificially induced to take up foreign DNA by incubating them in a culture medium that has a high concentration of calcium ions. This technique is use ...
Relative expression of wild-type and activated Ki
... human tumour tissues. In this method, the ratio of the two species of transcript is a direct linear function of the ratio of their amplified, reverse-transcribed cDNAs. We attribute the simplicity of this relationship to the fact that no correction is required to match the efficiencies of synthesis ...
... human tumour tissues. In this method, the ratio of the two species of transcript is a direct linear function of the ratio of their amplified, reverse-transcribed cDNAs. We attribute the simplicity of this relationship to the fact that no correction is required to match the efficiencies of synthesis ...
Figure 4.1
... consists of an a and a b half. The consensus sequence gives the most common base at each position. The "ancestral" sequence shows a sequence very closely related to the consensus sequence, which could have been the predecessor to the a and b units. (The satellite sequence is continuous, so that for ...
... consists of an a and a b half. The consensus sequence gives the most common base at each position. The "ancestral" sequence shows a sequence very closely related to the consensus sequence, which could have been the predecessor to the a and b units. (The satellite sequence is continuous, so that for ...
On the Mutational Topology of the Bacterial Genome
... (Figure 2). Over most of the range the gap sizes are smaller than expected, meaning that the mutations are clustered. A more interesting departure from random is revealed by the locations of the mutations. In Figure 3A the 1625 BPSs are collected in 46 bins, each bin approximately 100 kb wide, start ...
... (Figure 2). Over most of the range the gap sizes are smaller than expected, meaning that the mutations are clustered. A more interesting departure from random is revealed by the locations of the mutations. In Figure 3A the 1625 BPSs are collected in 46 bins, each bin approximately 100 kb wide, start ...
DNA repair
... nucleotide-excision • Same as previous except that It recognizes more varieties of damage Remove larger segments of DNA (10 -100s of bases) ...
... nucleotide-excision • Same as previous except that It recognizes more varieties of damage Remove larger segments of DNA (10 -100s of bases) ...
Section 3 - DNA Sequencing
... • Used for: gene prediction, protein structure/ function prediction, homology searching ...
... • Used for: gene prediction, protein structure/ function prediction, homology searching ...
Gatekeepers of pancreas: TEAD and YAP
... integrate extracellular signaling cues to control a timely cell differentiation program. The identified role for TEAD and YAP in this process opens up new research avenues towards disease development in humans. YAP has been described as an oncogene involved in several types of cancer, including panc ...
... integrate extracellular signaling cues to control a timely cell differentiation program. The identified role for TEAD and YAP in this process opens up new research avenues towards disease development in humans. YAP has been described as an oncogene involved in several types of cancer, including panc ...
poster - Andrew.cmu.edu - Carnegie Mellon University
... Figure 3, 4, and 5 showed the results of the three feature selection approaches used. In approach 1, we selected 796 features using information gain (Igain > 0.02) and ranked them using cross-entropy in Markov blanket filtering. In classification, at every step, the highest ranked feature was added ...
... Figure 3, 4, and 5 showed the results of the three feature selection approaches used. In approach 1, we selected 796 features using information gain (Igain > 0.02) and ranked them using cross-entropy in Markov blanket filtering. In classification, at every step, the highest ranked feature was added ...
Solid Tumour Section Soft tissue tumors: Aggressive angiomyxoma in Oncology and Haematology
... because these tumours may be stable with no or very limited growth over long periods of time. Several reported attempts using chemotherapy and radiotherapy as part of the treatment for AA have been disappointing, probably due to the low mitotic activity/growth fraction of cells. Most AA express oest ...
... because these tumours may be stable with no or very limited growth over long periods of time. Several reported attempts using chemotherapy and radiotherapy as part of the treatment for AA have been disappointing, probably due to the low mitotic activity/growth fraction of cells. Most AA express oest ...
Phylogenetic DNA profiling : a tool for the investigation of poaching
... Technologies, Australia). After an initial denaturation at 94°C for 2 min, the amplifications were performed using a “touchdown” amplification procedure consisting of 10 cycles of denaturation at 94°C for 45 sec, annealing for 10 sec at 62°C (decreasing 0.5°C per cycle) and extension at 72°C for 30 ...
... Technologies, Australia). After an initial denaturation at 94°C for 2 min, the amplifications were performed using a “touchdown” amplification procedure consisting of 10 cycles of denaturation at 94°C for 45 sec, annealing for 10 sec at 62°C (decreasing 0.5°C per cycle) and extension at 72°C for 30 ...
Document
... activates a systemically-delivered prodrug that inhibits DNA polymerase and blocks DNA replication in tumor cells. The herpes simplex virus thymidine kinase gene/ganciclovir prodrug (HSV-tk/GCV) system is an excellent example of the clinical application of GDEPT. The AAV-mediated HSV-tk/GCV therap ...
... activates a systemically-delivered prodrug that inhibits DNA polymerase and blocks DNA replication in tumor cells. The herpes simplex virus thymidine kinase gene/ganciclovir prodrug (HSV-tk/GCV) system is an excellent example of the clinical application of GDEPT. The AAV-mediated HSV-tk/GCV therap ...
a PDF version of the Genetics Learning Framework
... Students should be able to gather and evaluate experimental evidence, including qualitative and quantitative data. Students should be able to apply statistical methods when analyzing their data, and use patterns to construct a model. Students should be able to generate and interpret graphs displayin ...
... Students should be able to gather and evaluate experimental evidence, including qualitative and quantitative data. Students should be able to apply statistical methods when analyzing their data, and use patterns to construct a model. Students should be able to generate and interpret graphs displayin ...
division of molecular genetics
... autonomous element, aDart, and stabilized by segregating out of aDart under natural growth conditions. Not only pyl-stb but also the japonica cultivar Nipponbare carries no aDart, although they contain epigenetically silenced Dart elements that can be activated by the treatment of 5-azaC (Figure 3C) ...
... autonomous element, aDart, and stabilized by segregating out of aDart under natural growth conditions. Not only pyl-stb but also the japonica cultivar Nipponbare carries no aDart, although they contain epigenetically silenced Dart elements that can be activated by the treatment of 5-azaC (Figure 3C) ...
References - Proceedings of the Royal Society B
... DNA sequencing of the mitochondrial ND2 locus from our C. scorpioides laboratory matrilines has established that haplotypes in the A but not the B2 haplogroup possess a ClaI restriction site. ClaI digested ND2 amplicons were therefore used to confirm the mitochondrial haplotype of all putative sires ...
... DNA sequencing of the mitochondrial ND2 locus from our C. scorpioides laboratory matrilines has established that haplotypes in the A but not the B2 haplogroup possess a ClaI restriction site. ClaI digested ND2 amplicons were therefore used to confirm the mitochondrial haplotype of all putative sires ...
The PRICE of SILENT MUTATIONS
... Only in the 1980s did scientists realize that silent mutations could also affect protein production--at least in bacteria and yeast. A key discovery at the time was that the genes of those organisms did not use synonymous codons in equal numbers. When the bacterium Escherichia coli specifies the ami ...
... Only in the 1980s did scientists realize that silent mutations could also affect protein production--at least in bacteria and yeast. A key discovery at the time was that the genes of those organisms did not use synonymous codons in equal numbers. When the bacterium Escherichia coli specifies the ami ...
teachers notes - Learning on the Loop
... Why might a farmer using embryos produced by nuclear transfer from cows in elite herds see a much bigger jump in performance of the herd compared with a farmer using artificial insemination ? (Artificial insemination only supplies half the genes, but nuclear transfer, supplies all the genes of the d ...
... Why might a farmer using embryos produced by nuclear transfer from cows in elite herds see a much bigger jump in performance of the herd compared with a farmer using artificial insemination ? (Artificial insemination only supplies half the genes, but nuclear transfer, supplies all the genes of the d ...
PowerPoint 演示文稿
... by a loss-of-function. A null allele has total loss-of-function. Dominant alleles are caused by gain-of-function. 6 A mutation consists of a change in the sequence of A T and G C base pairs in DNA. A mutation in a coding sequence may change the sequence of amino acids in the corresponding protein. A ...
... by a loss-of-function. A null allele has total loss-of-function. Dominant alleles are caused by gain-of-function. 6 A mutation consists of a change in the sequence of A T and G C base pairs in DNA. A mutation in a coding sequence may change the sequence of amino acids in the corresponding protein. A ...
Genetics Notes
... credited for having discovered the shape of the DNA strand – it is a double helix. 3. Gregor Mendel experimented with pea plants to see how different traits (characteristics) are handed down from one generation to the next. He is known as the Father of Genetics. 4. Traits are the overall appearance, ...
... credited for having discovered the shape of the DNA strand – it is a double helix. 3. Gregor Mendel experimented with pea plants to see how different traits (characteristics) are handed down from one generation to the next. He is known as the Father of Genetics. 4. Traits are the overall appearance, ...
Oncogenomics
Oncogenomics is a relatively new sub-field of genomics that applies high throughput technologies to characterize genes associated with cancer. Oncogenomics is synonymous with ""cancer genomics"". Cancer is a genetic disease caused by accumulation of mutations to DNA leading to unrestrained cell proliferation and neoplasm formation. The goal of oncogenomics is to identify new oncogenes or tumor suppressor genes that may provide new insights into cancer diagnosis, predicting clinical outcome of cancers, and new targets for cancer therapies. The success of targeted cancer therapies such as Gleevec, Herceptin, and Avastin raised the hope for oncogenomics to elucidate new targets for cancer treatment.Besides understanding the underlying genetic mechanisms that initiates or drives cancer progression, one of the main goals of oncogenomics is to allow for the development of personalized cancer treatment. Cancer develops due to an accumulation of mutations in DNA. These mutations accumulate randomly, and thus, different DNA mutations and mutation combinations exist between different individuals with the same type of cancer. Thus, identifying and targeting specific mutations which have occurred in an individual patient may lead to increased efficacy of cancer therapy.The completion of the Human Genome Project has greatly facilitated the field of oncogenomics and has increased the abilities of researchers to find cancer causing genes. In addition, the sequencing technologies now available for sequence generation and data analysis have been applied to the study of oncogenomics. With the amount of research conducted on cancer genomes and the accumulation of databases documenting the mutational changes, it has been predicted that the most important cancer-causing mutations, rearrangements, and altered expression levels will be cataloged and well characterized within the next decade.Cancer research may look either on the genomic level at DNA mutations, the epigenetic level at methylation or histone modification changes, the transcription level at altered levels of gene expression, or the protein level at altered levels of protein abundance and function in cancer cells. Oncogenomics focuses on the genomic, epigenomic, and transcript level alterations in cancer.