Sequencing genomes
... (1991) it was a revolutionary way for gene identification. • EST is a short subsequence (200-800 bps) of cDNA sequence. They are unedited, randomly selected singlepass sequence reads derived from cDNA libraries. • They can be generated either from 5’ or from 3’ end. ...
... (1991) it was a revolutionary way for gene identification. • EST is a short subsequence (200-800 bps) of cDNA sequence. They are unedited, randomly selected singlepass sequence reads derived from cDNA libraries. • They can be generated either from 5’ or from 3’ end. ...
Classifying Nature
... strengthened and made considerably more resistant to such abuses as pathogen infection by the release of specific oligosaccharides and enzymes and by overlaying or impregnation with cutin, suberin, waxes or silica ...
... strengthened and made considerably more resistant to such abuses as pathogen infection by the release of specific oligosaccharides and enzymes and by overlaying or impregnation with cutin, suberin, waxes or silica ...
Linkage
... • Prototroph: “original” and “feed”, a wild type strain, one able to synthesize all needed compounds from a simple carbon source such as glucose. • Auxotroph: a mutant that has lost the ability to make some necessary organic compound; it must be added to the culture medium. • Bacteria show horizonta ...
... • Prototroph: “original” and “feed”, a wild type strain, one able to synthesize all needed compounds from a simple carbon source such as glucose. • Auxotroph: a mutant that has lost the ability to make some necessary organic compound; it must be added to the culture medium. • Bacteria show horizonta ...
Generation of mice with inducible T
... vivo, providing spatial (cell type specific) and temporal (inducible) control of gene expression at the same time. Here, we report the generation and characterization of a mouse mutant strain with inducible T cell-specific control of Cre, which we have developed to study the role of several genes in ...
... vivo, providing spatial (cell type specific) and temporal (inducible) control of gene expression at the same time. Here, we report the generation and characterization of a mouse mutant strain with inducible T cell-specific control of Cre, which we have developed to study the role of several genes in ...
No Slide Title
... A library is simply a collection of clones. Genomic clones are made from chromosomal DNA of some organism. A Genome Equivalent is the number of clones it would take for the size of the cloned fragments to equal the size of the genome of the organism. Fox example, consider a genome equivalent for mai ...
... A library is simply a collection of clones. Genomic clones are made from chromosomal DNA of some organism. A Genome Equivalent is the number of clones it would take for the size of the cloned fragments to equal the size of the genome of the organism. Fox example, consider a genome equivalent for mai ...
Gene Technology – Revision Pack (B6)
... STEP 1 – scientists add genes that make the bacteria resistant to antibiotics STEP 2 – the bacteria are then grown on an agar containing the antibiotic STEP 3 – scientists ONLY choose the bacteria that survive (that are resistant to the antibiotic) because they have taken up the antibiotic plasmid, ...
... STEP 1 – scientists add genes that make the bacteria resistant to antibiotics STEP 2 – the bacteria are then grown on an agar containing the antibiotic STEP 3 – scientists ONLY choose the bacteria that survive (that are resistant to the antibiotic) because they have taken up the antibiotic plasmid, ...
Metromnia poster | A2 - National Physical Laboratory
... need for fertilisers. However there has been strong Ros enf opposition to releasing such plants into the wild eld Im with both accurate and inaccurate claims made about their environmental impact. Only well controlled studies and accurate measurement will help the plant biotechnology industry to gro ...
... need for fertilisers. However there has been strong Ros enf opposition to releasing such plants into the wild eld Im with both accurate and inaccurate claims made about their environmental impact. Only well controlled studies and accurate measurement will help the plant biotechnology industry to gro ...
mnw2yr_lec1_2004
... cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein in principle) • Human DNA contains ~30,000 expressed genes • Deoxyri ...
... cells of a human body (an exception is, for example, red blood cells which have no nucleus and therefore no DNA) – a total of ~1022 nucleotides! • Many DNA regions code for proteins, and are called genes (1 gene codes for 1 protein in principle) • Human DNA contains ~30,000 expressed genes • Deoxyri ...
Gene Section GDF15 (growth differentiation factor 15) Atlas of Genetics and Cytogenetics
... also regulates the expression of GDF15/MIC-1 in vitro and in vivo. In prostate cancer cells, calcitriol induces GDF15/MIC-1 expression. Furthermore, it has been shown that the basal transcription of MIC-1 gene is regulated by Sp1 and Sp3. ...
... also regulates the expression of GDF15/MIC-1 in vitro and in vivo. In prostate cancer cells, calcitriol induces GDF15/MIC-1 expression. Furthermore, it has been shown that the basal transcription of MIC-1 gene is regulated by Sp1 and Sp3. ...
Document
... a check on the specificity of hybridization. For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different laboratories. They serve as landmarks on the developing physical map of a genome. They are used in shotgun sequencing, specifically ...
... a check on the specificity of hybridization. For this reason they are useful for constructing genetic and physical maps from sequence data reported from many different laboratories. They serve as landmarks on the developing physical map of a genome. They are used in shotgun sequencing, specifically ...
TF binding
... Gene Expression • Methylation at CpG islands often repress nearby gene expression • Many highly expressed genes have CpG methylation on their exons Some genes could be imprinted, so maternal and paternal copies have different DNA methylation From Ting Wang, Wash U ...
... Gene Expression • Methylation at CpG islands often repress nearby gene expression • Many highly expressed genes have CpG methylation on their exons Some genes could be imprinted, so maternal and paternal copies have different DNA methylation From Ting Wang, Wash U ...
Viral Genomes
... that is very often encoded by these genomes is a RNA-dependent RNA polymerase (RdRp). These polymerases are essential for the replication of both positive and negative strand ssRNAs. as well as dsRNAs. This is true for both monopartite and multipartite RNA viruses that show a range of 1-13 proteins. ...
... that is very often encoded by these genomes is a RNA-dependent RNA polymerase (RdRp). These polymerases are essential for the replication of both positive and negative strand ssRNAs. as well as dsRNAs. This is true for both monopartite and multipartite RNA viruses that show a range of 1-13 proteins. ...
Transcriptional gene silencing
... silencing homologous target genes 1998 Mello and Fire: -extension of above experiments, combination of sense and antisense RNA (= dsRNA) was 10 times more effective than single strand RNA ...
... silencing homologous target genes 1998 Mello and Fire: -extension of above experiments, combination of sense and antisense RNA (= dsRNA) was 10 times more effective than single strand RNA ...
ChromoWheel: a new spin on eukaryotic chromosome visualization
... service at Karolinska Institutet (http://chromowheel.cgb.ki.se). At this site, a ChromoWheel picture can be generated by submitting a file containing the data on which the picture ...
... service at Karolinska Institutet (http://chromowheel.cgb.ki.se). At this site, a ChromoWheel picture can be generated by submitting a file containing the data on which the picture ...
Comparative genomics
... Phylogenomics – obtain genes The software OrthoMCL is an example of a tool to obtain orthologous genes This software 1. Compares all the genes of all the organisms in a dataset (bidirectional Blast hit) ...
... Phylogenomics – obtain genes The software OrthoMCL is an example of a tool to obtain orthologous genes This software 1. Compares all the genes of all the organisms in a dataset (bidirectional Blast hit) ...
PPT - Department of Computer Science
... • Introduction: Identification of a transcription factor binding sites is an important aspect of the analysis of genetic regulation. Many programs have been developed for discovering the motif. ...
... • Introduction: Identification of a transcription factor binding sites is an important aspect of the analysis of genetic regulation. Many programs have been developed for discovering the motif. ...
Detailed characterization of the interactions between hepatitis C virus and host proteins.
... between HCV and host will determine the outcome of the viral infection. To characterize these processes in details, several novel viral-host interactions have been identified through yeast-twohybrid screen, proteomic and bioinformatic approaches. For the first part of the project, we aim to determin ...
... between HCV and host will determine the outcome of the viral infection. To characterize these processes in details, several novel viral-host interactions have been identified through yeast-twohybrid screen, proteomic and bioinformatic approaches. For the first part of the project, we aim to determin ...
Deadline: August 31, 2006
... the monoclonal antibodies established with GANPTg mice showed 10~100 fold increase of the affinity against model antigens as NP-hapten and the peptide epitope of the V3 region of HIV. GANP has three major domains with N-terminal RNA-primase, yeast Sac3 homology region, and C-terminal MCM3-binding/ac ...
... the monoclonal antibodies established with GANPTg mice showed 10~100 fold increase of the affinity against model antigens as NP-hapten and the peptide epitope of the V3 region of HIV. GANP has three major domains with N-terminal RNA-primase, yeast Sac3 homology region, and C-terminal MCM3-binding/ac ...
DNA Microarray - Montana State University
... GREEN – the healthy sample hybridized more than the diseased sample. ...
... GREEN – the healthy sample hybridized more than the diseased sample. ...
Pre-post test questions
... 15. Individuals with the diseases -thalassemia and sickle cell anemia both have mutations in the gene for hemoglobin. How could mutations in the same gene cause two different disease phenotypes? The different mutations in the DNA would cause different amino acids to be changed in the protein. The ...
... 15. Individuals with the diseases -thalassemia and sickle cell anemia both have mutations in the gene for hemoglobin. How could mutations in the same gene cause two different disease phenotypes? The different mutations in the DNA would cause different amino acids to be changed in the protein. The ...
Functional Non-Coding DNA
... • They are processed from much longer genes, or from introns within mRNA, by several molecular pathways • Micro-RNAs base-pair with complementary sequences within mRNA molecules, often in 3’ or 5’ UTR. • miRNA binding usually results in gene repression either via translational stalling or by trigger ...
... • They are processed from much longer genes, or from introns within mRNA, by several molecular pathways • Micro-RNAs base-pair with complementary sequences within mRNA molecules, often in 3’ or 5’ UTR. • miRNA binding usually results in gene repression either via translational stalling or by trigger ...
How We Know What Happened When
... mitochondrion. In fact, each mitochondrion has several copies of its own genome, and there are several hundred to several thousand mitochondria per cell. This means that the mitochondrial (mt) genome is highly amplified. While each cell contains only two copies of a given nuclear gene (one on each o ...
... mitochondrion. In fact, each mitochondrion has several copies of its own genome, and there are several hundred to several thousand mitochondria per cell. This means that the mitochondrial (mt) genome is highly amplified. While each cell contains only two copies of a given nuclear gene (one on each o ...
6. GENETICS 6.1 WARM-UP (p. 55) a. Genetics studies genes
... e. Mendel, Miescher, Bateson, De Lisi, Watson and Sanger are some of the most important scientists involved in genetic studies. 6.2 (p. 55) a. Genetics studies genes, heredity and the variation of organisms. b. The Human Genome Project is a project which aims at studying the human genome. c. It is t ...
... e. Mendel, Miescher, Bateson, De Lisi, Watson and Sanger are some of the most important scientists involved in genetic studies. 6.2 (p. 55) a. Genetics studies genes, heredity and the variation of organisms. b. The Human Genome Project is a project which aims at studying the human genome. c. It is t ...
Endogenous retrovirus
Endogenous retroviruses (ERVs) are endogenous viral elements in the genome that closely resemble and can be derived from retroviruses. They are abundant in the genomes of jawed vertebrates, and they comprise up to 5–8% of the human genome (lower estimates of ~1%). ERVs are a subclass of a type of gene called a transposon, which can be packaged and moved within the genome to serve a vital role in gene expression and in regulation. Researchers have suggested that retroviruses evolved from a type of transposable gene called a retrotransposon, which includes ERVs; these genes can mutate and instead of moving to another location in the genome they can become exogenous or pathogenic. This means that all ERVs may not have originated as an insertion by a retrovirus but that some may have been the source for the genetic information in the retroviruses they resemble.