Restriction Maps
... Over 3500 restriction enzymes have been discovered (about 600 of them are commercially available from various manufacturers), with a total of 253 distinct recognition sites. It is an interesting bioinformatics problem to scan a DNA sequence for restriction recognition sites. Often there are constrai ...
... Over 3500 restriction enzymes have been discovered (about 600 of them are commercially available from various manufacturers), with a total of 253 distinct recognition sites. It is an interesting bioinformatics problem to scan a DNA sequence for restriction recognition sites. Often there are constrai ...
Advances in selectable marker genes for plant transformation
... integrate and express transferred DNA. Genes encoding resistance to specific antibiotics or herbicides have proved particularly effective for selection and provide a means of rapidly identifying transformed cells, tissues and regenerated shoots that have integrated foreign DNA and that express the s ...
... integrate and express transferred DNA. Genes encoding resistance to specific antibiotics or herbicides have proved particularly effective for selection and provide a means of rapidly identifying transformed cells, tissues and regenerated shoots that have integrated foreign DNA and that express the s ...
Section A: DNA Cloning CHAPTER 20 DNA TECHNOLOGY AND
... • Practices that go back centuries, such as the use of microbes to make wine and cheese and the selective breeding of livestock, are examples of biotechnology. • Biotechnology based on the manipulation of DNA in vitro differs from earlier practices by enabling scientists to modify specific genes and ...
... • Practices that go back centuries, such as the use of microbes to make wine and cheese and the selective breeding of livestock, are examples of biotechnology. • Biotechnology based on the manipulation of DNA in vitro differs from earlier practices by enabling scientists to modify specific genes and ...
Chapter 5
... identified. The probe is designed to hybridize to the DNA of the clone that has been transferred to a membrane. The probe is labeled with radioactivity or another tag so that it can be easily detected and the proper clone identified and selected from the original plate. 10. A gene is inserted into a ...
... identified. The probe is designed to hybridize to the DNA of the clone that has been transferred to a membrane. The probe is labeled with radioactivity or another tag so that it can be easily detected and the proper clone identified and selected from the original plate. 10. A gene is inserted into a ...
Biotech Patenting in India - PHARMACEUTICALS EXPORT
... Vertebrate cells which can be propagated in vitro and which are capable upon growth in culture of producing erythropoietin in the medium of their growth in excess of 100 U of erythropoietin per 10.sup.6 cells in 48 hours as determined by radioimmunoassay, said cells comprising non-human DNA sequence ...
... Vertebrate cells which can be propagated in vitro and which are capable upon growth in culture of producing erythropoietin in the medium of their growth in excess of 100 U of erythropoietin per 10.sup.6 cells in 48 hours as determined by radioimmunoassay, said cells comprising non-human DNA sequence ...
ppt - Sol Genomics Network
... each of the 12 tomato chromosomes 2) to a degree of completion comparable to the standards of completion used to guide the international rice genome sequencing project (IRGSP, 2005) ---- e.g. anticipate 4 - 6 gaps ...
... each of the 12 tomato chromosomes 2) to a degree of completion comparable to the standards of completion used to guide the international rice genome sequencing project (IRGSP, 2005) ---- e.g. anticipate 4 - 6 gaps ...
Introduction
... gene expression in a person. These small RNA molecules play an important role in controlling gene activity by regulating a process known as translation. In translation, another type of RNA molecule, called a messenger RNA, which is produced by copying the genetic code of a gene, is used as a templat ...
... gene expression in a person. These small RNA molecules play an important role in controlling gene activity by regulating a process known as translation. In translation, another type of RNA molecule, called a messenger RNA, which is produced by copying the genetic code of a gene, is used as a templat ...
Biotechnology: Principles and Products
... The RoundUp Ready Story • Glyphosate is a broad-spectrum herbicide • Active ingredient in RoundUp herbicide • Kills all plants it comes in contact with • Inhibits a key enzyme (EPSP synthase) in ...
... The RoundUp Ready Story • Glyphosate is a broad-spectrum herbicide • Active ingredient in RoundUp herbicide • Kills all plants it comes in contact with • Inhibits a key enzyme (EPSP synthase) in ...
Nucleic acids
... • A nucleoside consists of a nitrogen base linked by a glycosidic bond to C1’ of a ribose or deoxyribose • Nucleosides are named by changing the nitrogen base ending to -osine for purines and –idine for pyrimidines • A nucleotide is a nucleoside that forms a phosphate ester with the C5’ OH group of ...
... • A nucleoside consists of a nitrogen base linked by a glycosidic bond to C1’ of a ribose or deoxyribose • Nucleosides are named by changing the nitrogen base ending to -osine for purines and –idine for pyrimidines • A nucleotide is a nucleoside that forms a phosphate ester with the C5’ OH group of ...
microbial genetics
... We will now look into specific examples of how these mechanisms are operational in E. coli to regulate its gene expression taking examples of three different OPERONS viz., lac (lactose) trp (tryptophan) and ara (arabinose). What are operons? For realizing a function, all genes involved in a given p ...
... We will now look into specific examples of how these mechanisms are operational in E. coli to regulate its gene expression taking examples of three different OPERONS viz., lac (lactose) trp (tryptophan) and ara (arabinose). What are operons? For realizing a function, all genes involved in a given p ...
2-3. Gene technology
... fibroblast cells) divide in vitro as many times as they do in the body. These cells are called primary cell culture. Primary culture can be produced using post-mitotic cells (neurons and myocardiocytes), too. (2) Tumor cell lines (isolated from tumors) have the capability for unlimited propagation. ...
... fibroblast cells) divide in vitro as many times as they do in the body. These cells are called primary cell culture. Primary culture can be produced using post-mitotic cells (neurons and myocardiocytes), too. (2) Tumor cell lines (isolated from tumors) have the capability for unlimited propagation. ...
Recombinant gene technology
... fibroblast cells) divide in vitro as many times as they do in the body. These cells are called primary cell culture. Primary culture can be produced using post-mitotic cells (neurons and myocardiocytes), too. (2) Tumor cell lines (isolated from tumors) have the capability for unlimited propagation. ...
... fibroblast cells) divide in vitro as many times as they do in the body. These cells are called primary cell culture. Primary culture can be produced using post-mitotic cells (neurons and myocardiocytes), too. (2) Tumor cell lines (isolated from tumors) have the capability for unlimited propagation. ...
Molecular Basis of Evolution
... As is clear from the above examples, molecular phylogenetics has become an important tool for studying the mechanism of evolution. ...
... As is clear from the above examples, molecular phylogenetics has become an important tool for studying the mechanism of evolution. ...
P450_L8_Structure of the Nucleic Acids
... The phosphate and sugars are very soluble in water, but the bases are not. Their insolubility does place strong constraints on the overall conformation of a large DNA or RNA molecule in solution. To be stable in water at neutral pH, the bases have to tuck themselves into the very center of a folded ...
... The phosphate and sugars are very soluble in water, but the bases are not. Their insolubility does place strong constraints on the overall conformation of a large DNA or RNA molecule in solution. To be stable in water at neutral pH, the bases have to tuck themselves into the very center of a folded ...
doc Feb 8th, 2010 notes
... Bacteriophage is a virus capable of infecting bacteria. For example, a bacteriophage (48, 502 bp) can infect E.Coli. o Bacteriophages, like plasmid, can be used as vectors and are capable of prolific replication within a cell. One third of its genome is not required for lytic growth, and can be repl ...
... Bacteriophage is a virus capable of infecting bacteria. For example, a bacteriophage (48, 502 bp) can infect E.Coli. o Bacteriophages, like plasmid, can be used as vectors and are capable of prolific replication within a cell. One third of its genome is not required for lytic growth, and can be repl ...
Hemoglobin Beta
... What is Hemoglobin? • Oxygen (and CO2) transporter for all vertebrates • Tetramer composed of four protein subunits (two alpha and two beta subunits) • Four Iron-containing haeme centers carry one oxygen molecule each • Beta-subunit mutations cause: – Sickle-cell anemia – Beta-thalessemia • 146 ami ...
... What is Hemoglobin? • Oxygen (and CO2) transporter for all vertebrates • Tetramer composed of four protein subunits (two alpha and two beta subunits) • Four Iron-containing haeme centers carry one oxygen molecule each • Beta-subunit mutations cause: – Sickle-cell anemia – Beta-thalessemia • 146 ami ...
histone proteins, the nucleosome and chromatin structure_9
... The nucleosome provides the first level of organization, giving a packing ratio of ~6. The second level of organization is the coiling of the series of nucleosomes into a helical array to constitute the fiber of diameter ~30 nm that is found in both interphase chromatin and mitotic chromosomes. In c ...
... The nucleosome provides the first level of organization, giving a packing ratio of ~6. The second level of organization is the coiling of the series of nucleosomes into a helical array to constitute the fiber of diameter ~30 nm that is found in both interphase chromatin and mitotic chromosomes. In c ...
Lecture 15: Translation and Transcription
... Amino acids must first be correctly linked with tRNA before the tRNA anticodon pairs with its complementary mRNA codon Aminoacyl-tRNA synthetase catalyzes the attachment of the amino acid to its tRNA a. Each amino acid has a unique aminoacyl-tRNA synthetase b. Reaction requires energy (i.e., endergo ...
... Amino acids must first be correctly linked with tRNA before the tRNA anticodon pairs with its complementary mRNA codon Aminoacyl-tRNA synthetase catalyzes the attachment of the amino acid to its tRNA a. Each amino acid has a unique aminoacyl-tRNA synthetase b. Reaction requires energy (i.e., endergo ...
Phylogenetic and genetic analysis of envelope gene of the
... A fresh wave of Dengue infection, particularly Dengue serotype 1and 3, have been observed all across India in recent times and has led to several fatalities. Since the surface situated envelope protein of the dengue virion is responsible for virus entry into the host cell, we have laid special empha ...
... A fresh wave of Dengue infection, particularly Dengue serotype 1and 3, have been observed all across India in recent times and has led to several fatalities. Since the surface situated envelope protein of the dengue virion is responsible for virus entry into the host cell, we have laid special empha ...
Protein Synthesis
... ribosome. Each group of 3 nitrogen bases in mRNA is called a codon. AUG is a special codon that is called an Initiation codon. It always codes for the amino acid Methionine. Check you codon wheel and identify the amino acids that the following codons code for: ...
... ribosome. Each group of 3 nitrogen bases in mRNA is called a codon. AUG is a special codon that is called an Initiation codon. It always codes for the amino acid Methionine. Check you codon wheel and identify the amino acids that the following codons code for: ...
Questions
... Some of the steps involved in Gene Cloning are given below i) Insertion of isolated gene to the vector ii) Introduction of recombinant vector to the host iii) Isolation of desired gene iv) Expression of recombinant gene in host v) Extraction of recombinant gene product The correct sequence of steps ...
... Some of the steps involved in Gene Cloning are given below i) Insertion of isolated gene to the vector ii) Introduction of recombinant vector to the host iii) Isolation of desired gene iv) Expression of recombinant gene in host v) Extraction of recombinant gene product The correct sequence of steps ...
Cells in culture.
... When a DNA probe is being used to find DNAs that are related, but not identical, in sequence, hybridization is performed at a lower temperature. This allows even imperfectly paired double helices to form. Only the lower-temperature hybridization conditions can be used to search for genes (C and E in ...
... When a DNA probe is being used to find DNAs that are related, but not identical, in sequence, hybridization is performed at a lower temperature. This allows even imperfectly paired double helices to form. Only the lower-temperature hybridization conditions can be used to search for genes (C and E in ...
Gene regulation in physiological stress
... The stability of mRNA transcripts, both pre-translation and during translation, is another level at which control can be exerted. Most mRNA transcripts proceed directly to the ribosomes where they are translated a variable number of times with half-lives for transcript stability ranging from seconds ...
... The stability of mRNA transcripts, both pre-translation and during translation, is another level at which control can be exerted. Most mRNA transcripts proceed directly to the ribosomes where they are translated a variable number of times with half-lives for transcript stability ranging from seconds ...
T-DNA Mutagenesis
... is when a mutation is created in such a way that death does not occur so as to observe the effects on the plant by the loss of a certain gene. In other words, a gene is knocked out and the plant is grown and observed for any differences between the mutant strain and the control strain. Thus facilita ...
... is when a mutation is created in such a way that death does not occur so as to observe the effects on the plant by the loss of a certain gene. In other words, a gene is knocked out and the plant is grown and observed for any differences between the mutant strain and the control strain. Thus facilita ...
Mechanisms for Creation of “Original Ancestor Genes”
... Fig. 8. New gene creation from original (SNS)n gene. New genes were easily produced from original (SNS)n genes through two routes as similarly as those described in Fig. 6, one is from sense sequence and the other is from antisense sequence of the original gene. “Original ancestor genes” could be pr ...
... Fig. 8. New gene creation from original (SNS)n gene. New genes were easily produced from original (SNS)n genes through two routes as similarly as those described in Fig. 6, one is from sense sequence and the other is from antisense sequence of the original gene. “Original ancestor genes” could be pr ...
Promoter (genetics)
In genetics, a promoter is a region of DNA that initiates transcription of a particular gene. Promoters are located near the transcription start sites of genes, on the same strand and upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long.