tRNA & Ribosomes
... Some Aminoacyl-tRNA Synthetases are known to have separate catalytic sites that release by hydrolysis inappropriate amino acids that are misacylated or mistransferred to tRNA. E.g., the aa-tRNA Synthetase for isoleucine (IleRS) a small percentage of the time activates the closely related amino acid ...
... Some Aminoacyl-tRNA Synthetases are known to have separate catalytic sites that release by hydrolysis inappropriate amino acids that are misacylated or mistransferred to tRNA. E.g., the aa-tRNA Synthetase for isoleucine (IleRS) a small percentage of the time activates the closely related amino acid ...
Protein Degradation, Volume 1 ch01_p 1..9
... this work, I was greatly helped by Aaron Ciechanover, who was my graduate student at that time. I have also received a lot of support, help, and great advice from Irwin Rose, in whose laboratory at Fox Chase Cancer Center I worked in a sabbatical year in 1978–79 and for many summers afterwards. In t ...
... this work, I was greatly helped by Aaron Ciechanover, who was my graduate student at that time. I have also received a lot of support, help, and great advice from Irwin Rose, in whose laboratory at Fox Chase Cancer Center I worked in a sabbatical year in 1978–79 and for many summers afterwards. In t ...
Proteomic Strategies to Analyze Cell
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
The Genome of Methanosarcina mazei
... are present: a bacterial type acetate kinase + phosphotransacetylase (not found in A. fulgidus and Halobacterium sp. NRC-1) and the archaeal ADP-forming acetyl-CoA-synthetase. Two copies of the genes encoding the CO dehydrogenase / acetyl-CoA-synthase with best blast e-values in Archaea were detecte ...
... are present: a bacterial type acetate kinase + phosphotransacetylase (not found in A. fulgidus and Halobacterium sp. NRC-1) and the archaeal ADP-forming acetyl-CoA-synthetase. Two copies of the genes encoding the CO dehydrogenase / acetyl-CoA-synthase with best blast e-values in Archaea were detecte ...
The Malaria Parasite`s Chloroquine Resistance Transporter is a
... transporter superfamily (Martin, Trueman, and Kirk 2003; Tran and Saier 2004). Here we present a detailed bioinformatic analysis of the protein and of the family and superfamily to which it belongs. Comparisons between PfCRT and members of the superfamily provide insight into the possible role of th ...
... transporter superfamily (Martin, Trueman, and Kirk 2003; Tran and Saier 2004). Here we present a detailed bioinformatic analysis of the protein and of the family and superfamily to which it belongs. Comparisons between PfCRT and members of the superfamily provide insight into the possible role of th ...
Chapter 9a - Richsingiser.com
... disease, prevalent in Africa and caused by the protozoan Trypanosoma brucei and similar organisms • No safe and effective drugs exist for this disease, but research has focused on the N-myristoyltransferase (NMT) that attaches myristic acid anchors to several essential cellular proteins in T. brucei ...
... disease, prevalent in Africa and caused by the protozoan Trypanosoma brucei and similar organisms • No safe and effective drugs exist for this disease, but research has focused on the N-myristoyltransferase (NMT) that attaches myristic acid anchors to several essential cellular proteins in T. brucei ...
when glucose is scarce
... 1. A repressible operon is one that is usually on; binding of a repressor to the operator shuts off transcription. 1. The trp operon is a repressible operon. trp operon ...
... 1. A repressible operon is one that is usually on; binding of a repressor to the operator shuts off transcription. 1. The trp operon is a repressible operon. trp operon ...
NAP57, a Mammalian Nucleolar Protein with a Putative Homolog
... complexes (Meier and Blobel, 1992). Similar tracks have now been observed with antibodies to the ribosomal protein S1 (Raska et al., 1992) and to the human immunodeficiency virus type 1 Nefprotein (Murti et al., 1993). These localization data are consistent with Nopp140 shuttling between the nucleol ...
... complexes (Meier and Blobel, 1992). Similar tracks have now been observed with antibodies to the ribosomal protein S1 (Raska et al., 1992) and to the human immunodeficiency virus type 1 Nefprotein (Murti et al., 1993). These localization data are consistent with Nopp140 shuttling between the nucleol ...
National Library of Medicine BuiMing 38A
... proteins evolve, their sequences are gradually transformed by biological events. The most common events include point mutations, where one amino acid is substituted with another; insertions, where a new amino acid is inserted into the sequence; and deletions, where an amino acid is deleted from a se ...
... proteins evolve, their sequences are gradually transformed by biological events. The most common events include point mutations, where one amino acid is substituted with another; insertions, where a new amino acid is inserted into the sequence; and deletions, where an amino acid is deleted from a se ...
Inhibitors
... Complementary to ATG start, 5’UTR or splice junction Prevents translation or splicing ...
... Complementary to ATG start, 5’UTR or splice junction Prevents translation or splicing ...
PROTEINS OF SPERM NUCLEI EXAMINED BY
... phosphate buffer control, all incubated at 37” for 3 hours. These enzymatic hydrolyses were made in an attempt to characterize the chromosome protein: trypsin cleaves the peptid chain only at sites with basic amino acids, chrymotrypsin breaks the chain only at sites with aromatic amino acids, while ...
... phosphate buffer control, all incubated at 37” for 3 hours. These enzymatic hydrolyses were made in an attempt to characterize the chromosome protein: trypsin cleaves the peptid chain only at sites with basic amino acids, chrymotrypsin breaks the chain only at sites with aromatic amino acids, while ...
5X All-In-One RT MasterMix
... An array of strategic mutations including those for the abrogation of RNase H activity, endow OneScript® RTase with its superior catalytic prowess. Nullifying the RNase H activity which is intrinsic to native RTase helps prevent RNA degradation during first-strand cDNA synthesis resulting in higher ...
... An array of strategic mutations including those for the abrogation of RNase H activity, endow OneScript® RTase with its superior catalytic prowess. Nullifying the RNase H activity which is intrinsic to native RTase helps prevent RNA degradation during first-strand cDNA synthesis resulting in higher ...
Proteomics of spermatogenesis: from protein lists to understanding
... work, testes from 8-, 18- and 45-day-old postnatal mice were comparatively studied and 44 proteins or variant forms were identified. However, these time points representing the first wave of spermatogenesis were not exact enough and this time span may have been too large. So, additional time points ...
... work, testes from 8-, 18- and 45-day-old postnatal mice were comparatively studied and 44 proteins or variant forms were identified. However, these time points representing the first wave of spermatogenesis were not exact enough and this time span may have been too large. So, additional time points ...
CHAPTER 16
... Answer: Tryptophan acts as a corepressor that causes the trp repressor to bind to the trp operon and repress transcription. FIGURE 16.12 Concept check: What type of bonding interactions cause the stem-loops to form? Answer: Hydrogen bonding between complementary sequences causes the stem-loops to fo ...
... Answer: Tryptophan acts as a corepressor that causes the trp repressor to bind to the trp operon and repress transcription. FIGURE 16.12 Concept check: What type of bonding interactions cause the stem-loops to form? Answer: Hydrogen bonding between complementary sequences causes the stem-loops to fo ...
13059_2010_2529_moesm2_esm
... T. virens and T. atroviride have a somewhat better pectin degradation machinery and possess at least 15 pectinolytic enzymes. A number of pectin degrading enzymes (GH78, 88, 105, PL1, CE8) are enriched in these two fungi in comparison to T. reesei. These additional proteins could constitute a small ...
... T. virens and T. atroviride have a somewhat better pectin degradation machinery and possess at least 15 pectinolytic enzymes. A number of pectin degrading enzymes (GH78, 88, 105, PL1, CE8) are enriched in these two fungi in comparison to T. reesei. These additional proteins could constitute a small ...
TALKING POINT The origin of the eukaryotic cell
... eubacteria. The letters A, E, N, P and 0 refer to sequences from archaebacteria (A), eukaryotes (cytosolic and endoplasmic reticulum; E), Gram-negative bacteria (N), Gram-positive bacteria (P) and eukaryotic organellar sequences [namely mitochondria (m) and chloroplasts (chl); 0], respectively. The ...
... eubacteria. The letters A, E, N, P and 0 refer to sequences from archaebacteria (A), eukaryotes (cytosolic and endoplasmic reticulum; E), Gram-negative bacteria (N), Gram-positive bacteria (P) and eukaryotic organellar sequences [namely mitochondria (m) and chloroplasts (chl); 0], respectively. The ...
Unraveling the mechanisms of RNA
... associations with a range of different RNA-binding proteins. These interactions are highly dynamic in nature and the balance between binding and dissociation events is likely to play a pivotal role in the function of RNA-binding proteins. Biacore™ systems are ideally suited to the detailed kinetic a ...
... associations with a range of different RNA-binding proteins. These interactions are highly dynamic in nature and the balance between binding and dissociation events is likely to play a pivotal role in the function of RNA-binding proteins. Biacore™ systems are ideally suited to the detailed kinetic a ...
Glimpses of a few literatures on snRNA
... responses induced by dsRNA. This process, called RNA interference, is sequence specific and can therefore be used to target gene expression. Nature Immunology 3, 597 - 599 (2002) doi:10.1038/ni0702-597 ...
... responses induced by dsRNA. This process, called RNA interference, is sequence specific and can therefore be used to target gene expression. Nature Immunology 3, 597 - 599 (2002) doi:10.1038/ni0702-597 ...
mRNA
... coli) and initiation factors are also required to start translation. The most common start codon is AUG which is read as methionine or, in bacteria, as formylmethionine. Alternative start codons (depending on the organism), include "GUG" or "UUG", which normally code for valine or leucine, respectiv ...
... coli) and initiation factors are also required to start translation. The most common start codon is AUG which is read as methionine or, in bacteria, as formylmethionine. Alternative start codons (depending on the organism), include "GUG" or "UUG", which normally code for valine or leucine, respectiv ...
Here is the Original File - University of New Hampshire
... acids such as RNA suggests that nucleic acids are highly flexible similar to proteins, and can undergo large-scale conformational rearrangements due to motions encoded in their structure or due to binding of triggering factors such as small metabolites or proteins. These observations warrant a detai ...
... acids such as RNA suggests that nucleic acids are highly flexible similar to proteins, and can undergo large-scale conformational rearrangements due to motions encoded in their structure or due to binding of triggering factors such as small metabolites or proteins. These observations warrant a detai ...
To support the hypothesis of an early RNA world, it is crucial to
... ribozyme isolate called MF (which contains sequence regions that can potentially fold into a previously selected pyrimidine synthase ribozyme). Ribozyme isolates were lightly mutated and then randomly recombined (4). Random recombination involves the digestion of ribozyme DNA, followed by ligation t ...
... ribozyme isolate called MF (which contains sequence regions that can potentially fold into a previously selected pyrimidine synthase ribozyme). Ribozyme isolates were lightly mutated and then randomly recombined (4). Random recombination involves the digestion of ribozyme DNA, followed by ligation t ...
In vivo interactions of higher plant Golgi matrix proteins by
... the endoplasmic reticulum (ER). It has long been known that the structure of the plant Golgi apparatus in higher plants is generally different to that in mammalian and fungal systems, comprising many discrete stacks of cisternae (or Golgi bodies) that appear more or less randomly distributed through ...
... the endoplasmic reticulum (ER). It has long been known that the structure of the plant Golgi apparatus in higher plants is generally different to that in mammalian and fungal systems, comprising many discrete stacks of cisternae (or Golgi bodies) that appear more or less randomly distributed through ...
Document
... 0. Like DNA, RNA contains 4 subunits (AUGC). It is less stable than DNA, so is not a storage media. 1. the DNA code a gene is copied into messenger RNA (mRNA) 2. mRNA is the version of the genetic code translated at the ribosome. 3. the ribosome is made up RNA (ribosomal RNA or rRNA) 4. T ...
... 0. Like DNA, RNA contains 4 subunits (AUGC). It is less stable than DNA, so is not a storage media. 1. the DNA code a gene is copied into messenger RNA (mRNA) 2. mRNA is the version of the genetic code translated at the ribosome. 3. the ribosome is made up RNA (ribosomal RNA or rRNA) 4. T ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.