Molecules of Life MBBS Prof. Fridoon
... Proteins: Structure Primary Structure: the sequence of amino acids bonded by peptide linkages (Diversity 20n) Secondary Structure: α helices and β pleated sheets (maintained by hydrogen bonds between atoms of the amino acid residues) ...
... Proteins: Structure Primary Structure: the sequence of amino acids bonded by peptide linkages (Diversity 20n) Secondary Structure: α helices and β pleated sheets (maintained by hydrogen bonds between atoms of the amino acid residues) ...
Lecture 1
... The leading strand is that strand of the DNA double helix that is oriented in a 5' to 3' manner. On the leading strand, a polymerase "reads" the DNA and adds nucleotides to it continuously. This polymerase is DNA polymerase III (DNA Pol III) in prokaryotes The lagging strand is that strand of the DN ...
... The leading strand is that strand of the DNA double helix that is oriented in a 5' to 3' manner. On the leading strand, a polymerase "reads" the DNA and adds nucleotides to it continuously. This polymerase is DNA polymerase III (DNA Pol III) in prokaryotes The lagging strand is that strand of the DN ...
Biochemistry 6/e
... Ribosomal RNA (rRNA)– part of the ribosome Transfer RNA (tRNA) Messenger RNA (mRNA)– sequence translated into protein sequence. Small nuclear RNA (snRNA) – involved in splicing (spliceosome) Micro RNA (mi RNA) – small RNA complementary to mRNA that inhibits translation of the mRNA – Small interferin ...
... Ribosomal RNA (rRNA)– part of the ribosome Transfer RNA (tRNA) Messenger RNA (mRNA)– sequence translated into protein sequence. Small nuclear RNA (snRNA) – involved in splicing (spliceosome) Micro RNA (mi RNA) – small RNA complementary to mRNA that inhibits translation of the mRNA – Small interferin ...
Chapter10_Outline
... • Typically, a genomic sequence contains many gaps that prevent the contigs from being assembled. • BAC clones are important because the sequences at the extreme ends of the cloned fragments give long-range information that allows adjacent contigs to be recognized and assembled in the correct orient ...
... • Typically, a genomic sequence contains many gaps that prevent the contigs from being assembled. • BAC clones are important because the sequences at the extreme ends of the cloned fragments give long-range information that allows adjacent contigs to be recognized and assembled in the correct orient ...
BIOLOGY
... undergo asexual reproduction by fragmentation, where segments from a parent plant regenerate a new plant. It is also possible to grow plants in culture from small explants. Another method is to culture plants from totipotent cells found in plant meristems. These plant cells can divide and differenti ...
... undergo asexual reproduction by fragmentation, where segments from a parent plant regenerate a new plant. It is also possible to grow plants in culture from small explants. Another method is to culture plants from totipotent cells found in plant meristems. These plant cells can divide and differenti ...
Genomic Annotation Lab Exercise By Jacob Jipp and Marian
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
Genomic Annotation Lab Exercise By Jacob Jipp and Marian
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
... sequence. A variety of queries can be used which enables sequence similarity to be identified at the protein and nucleotide level. Based on this sequence similarity, speculations can be made as to the homology of two genes. Evolutionarily speaking, these similarities can be interpreted as divergent ...
P 1 - Faperta UGM
... Involves cleavage of DNA with two different enzymes Involves ligation of specific linker pairs to the digested DNA Subsets of the DNA are then amplified by PCR The PCR products are then separated on acrylamide gel 128 linker combinations are readily available Therefore 128 subsets can be amplified ...
... Involves cleavage of DNA with two different enzymes Involves ligation of specific linker pairs to the digested DNA Subsets of the DNA are then amplified by PCR The PCR products are then separated on acrylamide gel 128 linker combinations are readily available Therefore 128 subsets can be amplified ...
Text S2
... “Metabolism” (16%) functional classes (figure A). Because the most abundant class of genes in the human genome is “Cellular Processes and Signaling” (figure B), the formation of CGs by parent genes belonging to this functional class by chance cannot be ruled out. Yet, it is noteworthy that this func ...
... “Metabolism” (16%) functional classes (figure A). Because the most abundant class of genes in the human genome is “Cellular Processes and Signaling” (figure B), the formation of CGs by parent genes belonging to this functional class by chance cannot be ruled out. Yet, it is noteworthy that this func ...
Molecular characterisation of RecQ homologues in Arabidopsis
... The rapidly growing sequence database of A.thaliana provides a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bl ...
... The rapidly growing sequence database of A.thaliana provides a powerful tool for identifying putative homologous proteins by database searches with sequence motives of genes of known function from different organisms. Such a database search using TBLASTN with the conserved helicase motives of the Bl ...
Initiation of transcription by Pol II Separate basal and activated
... Activated transcription by Pol II enhancers are sequences 5’ to TATAA transcriptional activators bind them • have distinct DNA binding and activation domains • activation domain interacts with mediator • helps assemble initiation complex on TATAA ...
... Activated transcription by Pol II enhancers are sequences 5’ to TATAA transcriptional activators bind them • have distinct DNA binding and activation domains • activation domain interacts with mediator • helps assemble initiation complex on TATAA ...
Biotechnology Lab (Kallas)
... thioredoxin (TRX) gene on plasmid pET32a, metal-chelating affinity chromatography may be used to purify the fusion protein, and the protease thrombin may be used to separate the protein of interest from the thioredoxin affinity tag. We will attempt to characterize the recombinant plasmids by sequenc ...
... thioredoxin (TRX) gene on plasmid pET32a, metal-chelating affinity chromatography may be used to purify the fusion protein, and the protease thrombin may be used to separate the protein of interest from the thioredoxin affinity tag. We will attempt to characterize the recombinant plasmids by sequenc ...
"Big Data" from RNA
... 6. NIST : data which exceed(s) the capacity or capability of current or conventional methods and systems. http://www.technologyreview.com/view/519851/the-big-data-conundrum-how-to-define-it/ ...
... 6. NIST : data which exceed(s) the capacity or capability of current or conventional methods and systems. http://www.technologyreview.com/view/519851/the-big-data-conundrum-how-to-define-it/ ...
(NEU1) gene in two patients of sialidosis in India
... neuraminidase-1 protein has been previously reported, an arginine to cysteine substitution (R294C) at the same site has not been reported before, and the 1191delG is a novel, hitherto unreported mutation8. Homology alignment showed that the amino acid arginine was conserved at the 294th position in ...
... neuraminidase-1 protein has been previously reported, an arginine to cysteine substitution (R294C) at the same site has not been reported before, and the 1191delG is a novel, hitherto unreported mutation8. Homology alignment showed that the amino acid arginine was conserved at the 294th position in ...
Supplement Material…
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
Chapter 12 Presentation-The Cell Cycle
... the “guardian of the genome”-it acts as a gate keeper. • The p53 gene makes p53 protein which does many different things. •It can arrest cell proliferation by holding the cell cycle at the G1/S checkpoint when DNA damage is p53 17q13.1: This gene encodes a tumor suppressor protein containing recogni ...
... the “guardian of the genome”-it acts as a gate keeper. • The p53 gene makes p53 protein which does many different things. •It can arrest cell proliferation by holding the cell cycle at the G1/S checkpoint when DNA damage is p53 17q13.1: This gene encodes a tumor suppressor protein containing recogni ...
Molecular Cloning and Characterization of a Novel Human Glycine-N-acyltransferase Gene GLYATL1, Which Activates Transcriptional Activity of HSE Pathway
... insights into the evolution and genetics of these important detoxifying systems. Third, it may relate to the pathogenesis and possibly the treatment of certain organic acidemias. Finally, it does provide a useful model for studying mechanisms of enzymatic transfer reactions. During the past five dec ...
... insights into the evolution and genetics of these important detoxifying systems. Third, it may relate to the pathogenesis and possibly the treatment of certain organic acidemias. Finally, it does provide a useful model for studying mechanisms of enzymatic transfer reactions. During the past five dec ...
SynCAM2a ΔPDZ Δ4.1B ΔPDZ - University of Oregon (SPUR)
... Subclone 4 SynCAM2a constructs (full-length, and 3 deletions: 4.1B, PDZ, and 2X [both protein binding domains] deletions into a vector with a fluorescent tag. Microinject zebrafish embryos with the 4 constructs. Stain known pre and postsynaptic proteins through immunohistochemistry (IHC) to de ...
... Subclone 4 SynCAM2a constructs (full-length, and 3 deletions: 4.1B, PDZ, and 2X [both protein binding domains] deletions into a vector with a fluorescent tag. Microinject zebrafish embryos with the 4 constructs. Stain known pre and postsynaptic proteins through immunohistochemistry (IHC) to de ...
File
... When covering microsatellites, present the topic using a series of diagrams to help students understand this concept. When covering how PCR works, present the topic using a series of diagrams to help students understand this concept. Some students may have difficulties understanding why DNA would mo ...
... When covering microsatellites, present the topic using a series of diagrams to help students understand this concept. When covering how PCR works, present the topic using a series of diagrams to help students understand this concept. Some students may have difficulties understanding why DNA would mo ...
Parallel Data Mining of microarray biological data
... In the last fifty years, research in biology pointed out that life information of all beings is embedded into the DNA. DNA is a complex molecule, the fundamental components of which are the nucleotides, called also bases: A (adenine), T (thymine), C (citosine), G (guanine). This molecule is structur ...
... In the last fifty years, research in biology pointed out that life information of all beings is embedded into the DNA. DNA is a complex molecule, the fundamental components of which are the nucleotides, called also bases: A (adenine), T (thymine), C (citosine), G (guanine). This molecule is structur ...