Slide 1
... • Insulator sequences are cis-acting sequences located between enhancers and the promoters of genes that need to be protected from the action of the enhancers • Insulators ensure that only the target gene is regulated by the enhancer • Insulators may allow formation of DNA loops that contain the enh ...
... • Insulator sequences are cis-acting sequences located between enhancers and the promoters of genes that need to be protected from the action of the enhancers • Insulators ensure that only the target gene is regulated by the enhancer • Insulators may allow formation of DNA loops that contain the enh ...
Introducing DOTUR, a Computer Program for Defining Operational
... Richness comparison between two soil samples using DOTUR • Result: The number of observed OTUs from the Amazonian soil falls within the 95 confidence interval (CI) of the Scottish soil with 98 sequences sampled ...
... Richness comparison between two soil samples using DOTUR • Result: The number of observed OTUs from the Amazonian soil falls within the 95 confidence interval (CI) of the Scottish soil with 98 sequences sampled ...
IFU COL G 18 set 2013
... specificity, MBP-ColG is especially indicated for the isolation of primary cells from liver, pancreas, heart, cartilage and stem cells from adipose tissue and others. In these applications we recommend to use a combination of ColG / ColH in specific molar ratio in order to obtain an optimal collagen ...
... specificity, MBP-ColG is especially indicated for the isolation of primary cells from liver, pancreas, heart, cartilage and stem cells from adipose tissue and others. In these applications we recommend to use a combination of ColG / ColH in specific molar ratio in order to obtain an optimal collagen ...
Genetic Markers of E. coli
... Overproduction of the lac repressor protein Leads to high levels of the lac repressor protein, inhibiting transcription from the lac promoter. lacY Galactoside permease mutation Blocks lactose utilization. lacZ∆M15 Partial deletion of β-d-galactosidase gene Allows complementation of β-galactosidase ...
... Overproduction of the lac repressor protein Leads to high levels of the lac repressor protein, inhibiting transcription from the lac promoter. lacY Galactoside permease mutation Blocks lactose utilization. lacZ∆M15 Partial deletion of β-d-galactosidase gene Allows complementation of β-galactosidase ...
Genes Are DNA
... A nucleotide consists of a nucleoside linked to a phosphate group on either the 5′ or 3′ position of the (deoxy)ribose. Successive (deoxy)ribose residues of a polynucleotide chain are joined by a phosphate group ...
... A nucleotide consists of a nucleoside linked to a phosphate group on either the 5′ or 3′ position of the (deoxy)ribose. Successive (deoxy)ribose residues of a polynucleotide chain are joined by a phosphate group ...
DNA Transcription and Translation Project
... DNA Transcription and Translation Activity This assignment is due on the day of the Transcription/Translation test. No late work will be accepted. All organisms use proteins to grow and function. These proteins are made up of thousands of amino acids which were created through the processes of DNA t ...
... DNA Transcription and Translation Activity This assignment is due on the day of the Transcription/Translation test. No late work will be accepted. All organisms use proteins to grow and function. These proteins are made up of thousands of amino acids which were created through the processes of DNA t ...
Basics of BLAST - GEP Community Server
... -Good balance of sensitivity and speed -Reliable -Flexible ...
... -Good balance of sensitivity and speed -Reliable -Flexible ...
... was cultured at 37°C in Luria-Bertani (LB) medium or on LB agar plates. The acyl-HSL used in this study was 3-oxohexanoyl-DL-homoserine lactone (3OC6HSL; Sigma Aldrich). Antibiotics were added at concentrations of 50 g/ml kanamycin and 100 g/ml chloramphenicol to maintain the plasmids. Constructio ...
The 18-kD Protein That Binds to the Chloroplast DNA
... the 18-kD band as well as with several other bands of higher molecular weight on a protein blot prepared from a high-salt extract of an extensively purified thylakoid membrane of C. reinhardtii. It is known that NADH dehydrogenase may be dissociated into subunits by a wide variety of treatments (Rag ...
... the 18-kD band as well as with several other bands of higher molecular weight on a protein blot prepared from a high-salt extract of an extensively purified thylakoid membrane of C. reinhardtii. It is known that NADH dehydrogenase may be dissociated into subunits by a wide variety of treatments (Rag ...
protein - Blog UB - Universitas Brawijaya
... • Proteins can be described as having several layers of structure. At the lowest level, the primary structure of proteins are nothing more that the amino acids which compose the protein, and how those proteins are bonded to each other. The bonds between proteins are called peptide bonds, and they ca ...
... • Proteins can be described as having several layers of structure. At the lowest level, the primary structure of proteins are nothing more that the amino acids which compose the protein, and how those proteins are bonded to each other. The bonds between proteins are called peptide bonds, and they ca ...
Molecular Evolution of Nitrate Reductase Genes
... All eukaryotic NRs have three highly conserved functional domains binding FAD, heme, and molybdenum cofactor (MoCo) (Kleinhofs et al. 1989). The three functional domains are separated by two short hinge regions (Kubo et al. 1988). They are encoded in a linear order with the MoCo region at the N-term ...
... All eukaryotic NRs have three highly conserved functional domains binding FAD, heme, and molybdenum cofactor (MoCo) (Kleinhofs et al. 1989). The three functional domains are separated by two short hinge regions (Kubo et al. 1988). They are encoded in a linear order with the MoCo region at the N-term ...
Nucleotide sequence of the thioredoxin gene from
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
... the sequence of another Sau3A fragment (Fig. 2) from the thioredoxin gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separatio ...
Expression of gene encoding immunodominant merozoite surface
... N and C termini. The size of the product was 733 bp (Fig.1) and the concentration of the purified product was 12 ng/µL. A product of 876 bp, comprising full length of the gene (not shown in Fig. 1) could not be expressed using the prokaryotic expression system probably due to the toxicity of the hyd ...
... N and C termini. The size of the product was 733 bp (Fig.1) and the concentration of the purified product was 12 ng/µL. A product of 876 bp, comprising full length of the gene (not shown in Fig. 1) could not be expressed using the prokaryotic expression system probably due to the toxicity of the hyd ...
Request pdf - University of Cambridge
... In man, the spectral shift between the middle-wave (MW) aud long-wave (LW) visual pigments is largely achieved by amino acid substitution at two codons, both located in exon 5. A third amino acid site coded by exon 3 is polymorphic between pigments. We have studied the equivalent regions of the cone ...
... In man, the spectral shift between the middle-wave (MW) aud long-wave (LW) visual pigments is largely achieved by amino acid substitution at two codons, both located in exon 5. A third amino acid site coded by exon 3 is polymorphic between pigments. We have studied the equivalent regions of the cone ...
Regulation of transcription by Saccharomyces cerevisiae 14-3
... introduction of a mutated bmh2 allele [24]. We used this mutant to identify extragenic suppressor mutations bypassing the requirement of active 14-3-3 proteins. Recessive mutations in RTG3 and SIN4 resulted in growth at the restrictive temperature. RTG3 encodes a basic helix–loop–helix transcription ...
... introduction of a mutated bmh2 allele [24]. We used this mutant to identify extragenic suppressor mutations bypassing the requirement of active 14-3-3 proteins. Recessive mutations in RTG3 and SIN4 resulted in growth at the restrictive temperature. RTG3 encodes a basic helix–loop–helix transcription ...
Universal Features of Post-Transcriptional Gene Regulation Are
... includes 16 major factors, including proteins with homologies to components of metazoan P granules and archaeal proteins. Containing translationally silent transcripts, this mRNP integrates eIF4E and poly(A)-binding protein but excludes P body RNA degradation factors and translation-initiation promo ...
... includes 16 major factors, including proteins with homologies to components of metazoan P granules and archaeal proteins. Containing translationally silent transcripts, this mRNP integrates eIF4E and poly(A)-binding protein but excludes P body RNA degradation factors and translation-initiation promo ...
SP1 Protein production order form
... If yes, how many, which type and which order: Does the construct contain TEV cleavage site: ...
... If yes, how many, which type and which order: Does the construct contain TEV cleavage site: ...
Genomic organization of infectious salmon anaemia virus
... open reading frame encoding the P1, PB1, NP, P2, P3 and HA proteins, respectively. Segment 7 encoded the P4/P5 proteins and segment 8 encoded the P6/P7 proteins. Seven virion proteins with molecular masses between 25 and 72 kDa were found by SDS–PAGE analysis. The 72 and 42 kDa proteins were immunor ...
... open reading frame encoding the P1, PB1, NP, P2, P3 and HA proteins, respectively. Segment 7 encoded the P4/P5 proteins and segment 8 encoded the P6/P7 proteins. Seven virion proteins with molecular masses between 25 and 72 kDa were found by SDS–PAGE analysis. The 72 and 42 kDa proteins were immunor ...
- Wiley Online Library
... to light decreased transcript levels in ME cells (Lai et al., 2002). Interestingly, cis-elements that affect BS-specific expression of ME are different for F. bidentis and a close C4 relative, F. trinervia. In F. bidentis, the endogenous NADPME gene may require elements within the coding region at t ...
... to light decreased transcript levels in ME cells (Lai et al., 2002). Interestingly, cis-elements that affect BS-specific expression of ME are different for F. bidentis and a close C4 relative, F. trinervia. In F. bidentis, the endogenous NADPME gene may require elements within the coding region at t ...
Article Parallel Histories of Horizontal Gene
... Identification of Preferentially Expressed Genes in the Psyllid Bacteriome by mRNA-Seq Deep strand-specific sequencing of polyadenylated transcripts (mRNA-seq) in P. venusta nymphs isolated from hackberry galls was used to broadly identify and characterize psyllid genes. Furthermore, by comparing ex ...
... Identification of Preferentially Expressed Genes in the Psyllid Bacteriome by mRNA-Seq Deep strand-specific sequencing of polyadenylated transcripts (mRNA-seq) in P. venusta nymphs isolated from hackberry galls was used to broadly identify and characterize psyllid genes. Furthermore, by comparing ex ...
Proteomics pathway Most common properties of proteins
... Objetive to allow an efficient separation of the greater number of proteins in two dimensions. This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes denaturing ...
... Objetive to allow an efficient separation of the greater number of proteins in two dimensions. This procedure is very important and must: must: • Solubilise as many proteins as possible including hydrophobic species • Prevent protein aggregates and hydrophobic interactions. This includes denaturing ...
Medical and Ethical Implications of Inexpensive Genome
... Early Detection of Abnormal Genes - Cystic Fibrosis is caused by a mutation in the CFTR (cystic fibrosis transmembrane conductance regulator) gene - A single mutated gene causes other diseases such as Huntington’s disease and Duchenne muscular dystrophy, and those genes have already been identified. ...
... Early Detection of Abnormal Genes - Cystic Fibrosis is caused by a mutation in the CFTR (cystic fibrosis transmembrane conductance regulator) gene - A single mutated gene causes other diseases such as Huntington’s disease and Duchenne muscular dystrophy, and those genes have already been identified. ...
Supporting Information
... encodes a 669 amino acid-long protein, is more abundant than At3g16857.2 that encodes a 690 amino acid-long protein. Relative transcript levels were determined using qPCR analyses with GADPH as a reference gene essentially as described (Li et al. 2013). The ARR1-specific primer sequences are present ...
... encodes a 669 amino acid-long protein, is more abundant than At3g16857.2 that encodes a 690 amino acid-long protein. Relative transcript levels were determined using qPCR analyses with GADPH as a reference gene essentially as described (Li et al. 2013). The ARR1-specific primer sequences are present ...