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Griffith`s Experiment
Griffith`s Experiment

... 2. RNA polymerase “reads” 1 strand of DNA to produce a strand of messenger RNA (mRNA). 3. Complementary RNA nucleotides pair across from the DNA nucleotides (A-U; G-C, C-G; T-A) 4. RNA polymerase links the nucleotides together. 5. The process continues until the end of the gene is reached ...
Gene regulation in physiological stress
Gene regulation in physiological stress

... over the expression of these genes is actually at the translational level. Other layers of regulatory controls are applied to translation. Because protein synthesis is energyexpensive (needing ~5 ATP equivalents per peptide bond formed), the rate of protein synthesis must be closely matched with the ...
File
File

...  The every 3 bases on the RNA strand code ...
Analysis of microarray data
Analysis of microarray data

... • Microarrays are chips which measure whether genes are switched on or off in cells. • They can be used to detect sets of genes responsible for genetic diseases such as cancer. • This lecture: – introduce microarray technology – discuss a few applications – introduce statistical and computational te ...
cell cycle
cell cycle

... reactions to incorporate new nucleotides into the complementary strands. The cycle is then repeated over and over until there are millions of copies of the target DNA. a. Predict why this bacterial polymerase is used instead of a human polymerase. ...
Nucleic Acids - University of California, Davis
Nucleic Acids - University of California, Davis

... stacked onto other base pairs in an RNA structure. Contiguous base pairs are called stems. • Unlike DNA, RNA is typically produced as a single stranded molecule which then folds intra-molecularly to form a number of short base-paired stems. This base-paired structure is called RNA secondary structur ...
tRNA, rRNA, and RNAi Transfer RNA (tRNA) Characteristics of tRNA
tRNA, rRNA, and RNAi Transfer RNA (tRNA) Characteristics of tRNA

... TΨC: Thymine, pseudouracil, cytosine ...
NBT Briefing - EcoNexus December 2015
NBT Briefing - EcoNexus December 2015

... As  regards  NBTs,  it  is  of  concern  that  many  efforts  seem  designed  primarily  to  avoid  having   to  go  through  the  regulatory  process  for  GMOs,  whilst  choosing  names  that  make  it  difficult   for  the  public ...
10.3 Protein Synthesis
10.3 Protein Synthesis

... • The language of mRNA is called the Genetic Code (A, G, U, C)  (contains only 4 letters) • It is the matching of the RNA sequence to the correct amino acid to make proteins. • It is based on codons, which are 3 bases together on an mRNA chain. • Each codon codes for a specific amino acid • There a ...
DNA, RNA and Protein Structure Prediction
DNA, RNA and Protein Structure Prediction

... The two chains of DNA form a double helix. The base pairs are inside the helix and the sugarphosphate backbones are outside it. In eycaryotes, the DNA is packaged into chromosomes. DNA is wrapped around histones. DNA and core histones form nucleosomes, which are packed into a compact chromatin fiber ...
Yellow Line Walk-through
Yellow Line Walk-through

...  Non-spliced genes, which are characteristic of prokaryotes, are also found in eukaryotes.  Even in a spliced gene, the protein-coding information may be organized as Open Reading Frame (ORF).  Most eukaryotic genes are spliced, whereby intervening segments (introns) are removed and the remaining ...
p53
p53

... • About a half dozen DNA changes must occur for a cell to become fully cancerous. • These usually include the appearance of at least one active oncogene and the mutation or loss of several tumor-suppressor genes. ...
lac Operon - Mediatech, Inc.
lac Operon - Mediatech, Inc.

... An operon is a unit of gene expression and a transcriptionally-regulated system. The lac operon is responsible for producing the proteins that control the uptake of lactose for use as a carbon energy source when glucose is not available to the cell. It consists of three structural genes and a repres ...
lecture07_13
lecture07_13

... For each position l in the input sequence, check if substring starting at position l matches the motif. Example: find the consensus motif NTAHAWT in the promoter of a gene >promoter of gene A ...
THE GENETIC PROCESS CHAPTER 4
THE GENETIC PROCESS CHAPTER 4

... The discussion thus far describes the conversion of DNA information for the synthesis of proteins. The discussion is incomplete without consideration of another important process, DNA replication. Replication is the process whereby a DNA molecule duplicates to yield identical DNA molecules. The dupl ...
Poster
Poster

... anchoring proteins (AKAPs) bind and help localize PKA to specific areas. The RIIa domain in PKA provides a shallow groove for an amphipathic helix of AKAP to bind via interactions of hydrophobic side chains. A similar binding motif is found in the DPY-30 domain, which suggests this domain may also p ...
Today is Wednesday, February 3rd, 2010
Today is Wednesday, February 3rd, 2010

... • First, how does all this stuff start up? That’s our focus for today. – BTW, we’re talking both prokaryotes and eukaryotes! ...
frame-shift mutation
frame-shift mutation

... • First, how does all this stuff start up? That’s our focus for today. – BTW, we’re talking both prokaryotes and eukaryotes! ...
Cloning Using Plasmid Vectors
Cloning Using Plasmid Vectors

... Unique restriction sites May have additional features such as mob sites, RNA polymerase promoters, etc. ...
The human genome of is found where in the human body?
The human genome of is found where in the human body?

... PCR can amplify DNA, a great help in forensics and diagnostics • Other uses: modifying genes, detecting genes • How it works: 1. High heat breaks H-bonds between base pairs 2. Primers bind to sequence of interest 3. Heat-tolerant Taq polymerase copies 4. Goto 1 5. Each round doubles the amount of D ...
DNA REPLICATION Review of DNA Structure
DNA REPLICATION Review of DNA Structure

... RNA primer complementary to the DNA templates • After formation of the primer, DNA polymerase III – elongates the new strand by adding nucleotides to the 3’end (~50 per ...
Biology Notebook
Biology Notebook

... affect other viruses. They are only made of the protein coat (capsid) of the virus. It “tells” the genetic sequence of the virus, to the cells of the organism that is infected.  Antibiotics cannot destroy viruses, because viruses are metabolically inert, they aren’t alive.  They were discovered by ...
pdf format - Faculty members Homepages
pdf format - Faculty members Homepages

... accession no. CAB63048) is a sequence directly deposited in the database. In addition, there are several potential pseudogenes in the human genome based on their apparent lack of intron sequences on chromosomes 1, 10, and 11. The first 164 aa of HDAC4 have a perfect match on chromosome 3, and part o ...
Functional and phylogenetic analyses of chromosome 21 promoters
Functional and phylogenetic analyses of chromosome 21 promoters

... composition of expressed genes changes considerably among cell types and in response to physiological and environmental conditions [1-5]. Eukaryotic genomes contain on the order of 0.5–5x104 genes. To allow precise spacio-temporal gene expression, a particularly complex system of regulatory mechanis ...
File
File

... Carbon, hydrogen, nitrogen and sulphur are elements found in living cells. Which is the least ...
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Transcriptional regulation

In molecular biology and genetics, transcriptional regulation is the means by which a cell regulates the conversion of DNA to RNA (transcription), thereby orchestrating gene activity. A single gene can be regulated in a range of ways, from altering the number of copies of RNA that are transcribed, to the temporal control of when the gene is transcribed. This control allows the cell or organism to respond to a variety of intra- and extracellular signals and thus mount a response. Some examples of this include producing the mRNA that encode enzymes to adapt to a change in a food source, producing the gene products involved in cell cycle specific activities, and producing the gene products responsible for cellular differentiation in higher eukaryotes.The regulation of transcription is a vital process in all living organisms. It is orchestrated by transcription factors and other proteins working in concert to finely tune the amount of RNA being produced through a variety of mechanisms. Prokaryotic organisms and eukaryotic organisms have very different strategies of accomplishing control over transcription, but some important features remain conserved between the two. Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. In a hypothetical example, the factors A and B might regulate a distinct set of genes from the combination of factors A and C. This combinatorial nature extends to complexes of far more than two proteins, and allows a very small subset (less than 10%) of the genome to control the transcriptional program of the entire cell.
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