Reagents - NutriTech
... Dr Jennifer Duff and Dr Alison Richmond Post docs [email protected] 16 April 2009 When citing this SOP you should acknowledge both NuGO and the appropriate NuGO partner institution that has made the SOP available. Please use a form of words such as: We used the NuGO Standard Operating Procedur ...
... Dr Jennifer Duff and Dr Alison Richmond Post docs [email protected] 16 April 2009 When citing this SOP you should acknowledge both NuGO and the appropriate NuGO partner institution that has made the SOP available. Please use a form of words such as: We used the NuGO Standard Operating Procedur ...
limited warranty
... µl of appropriate growth medium containing serum and antibiotics on the day before transfection. Incubate the cells at 37 °C and 5% CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serum-containing medium is replaced with 360 µl Opti-Medium (In ...
... µl of appropriate growth medium containing serum and antibiotics on the day before transfection. Incubate the cells at 37 °C and 5% CO2. The plate should be 60~80% confluent on the day of transfection. One hour before transfection, the serum-containing medium is replaced with 360 µl Opti-Medium (In ...
![DNA_fingerprinting_etrophoresisPowerPoint[2]](http://s1.studyres.com/store/data/008317714_1-e4b397f2592ff793b5b728465432e82b-300x300.png)
“Ins and Outs” of Restrictions Enzymes
... DNA Enzymology History • 1953: molecular structure of DNA described • 1955: DNA polymerase • 1966: DNA ligase • 1968: 1st sequence specific restriction ...
... DNA Enzymology History • 1953: molecular structure of DNA described • 1955: DNA polymerase • 1966: DNA ligase • 1968: 1st sequence specific restriction ...
Restriction enzymes
... These are often a symmetrical series of four to eight bases on both strands running in opposite directions. If the restriction site on one strand is 3’-CTTAAG-5’, the complementary strand is 5’-GAATTC-3’. ...
... These are often a symmetrical series of four to eight bases on both strands running in opposite directions. If the restriction site on one strand is 3’-CTTAAG-5’, the complementary strand is 5’-GAATTC-3’. ...
Comparing DNA
... recognizes a particular sequence of bases on the DNA helix and cuts the DNA at that point. The DNA pieces, or fragments, are different sizes and will travel through the gel at different speeds. The smaller the fragment, the faster it can travel and vice versa. (It’s like having a 180 lb quarterback ...
... recognizes a particular sequence of bases on the DNA helix and cuts the DNA at that point. The DNA pieces, or fragments, are different sizes and will travel through the gel at different speeds. The smaller the fragment, the faster it can travel and vice versa. (It’s like having a 180 lb quarterback ...
Agarose Gel Electrophoresis Description An electrophoresis
... recovery of DNA. Lower voltages, coupled with longer running times, provide optimum resolution, such as that required for Southern Blots or forensic applications. Pulsed-field electrophoresis can be used to separate very large DNA fragments. The most common stain is ethidium bromide, which intercala ...
... recovery of DNA. Lower voltages, coupled with longer running times, provide optimum resolution, such as that required for Southern Blots or forensic applications. Pulsed-field electrophoresis can be used to separate very large DNA fragments. The most common stain is ethidium bromide, which intercala ...
Replication/Transcription/Translation
... 1. Name the 3 essential enzymes for replication. DNA helicase, polymerase, and ligase 2. Describe the semi-conservative model. The parent strand acts at the model for the new daughter ...
... 1. Name the 3 essential enzymes for replication. DNA helicase, polymerase, and ligase 2. Describe the semi-conservative model. The parent strand acts at the model for the new daughter ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""