Important Experiments
... How the genes on DNA control protein production needed for a cell’s growth and function. Summary of how proteins are made: 38. _______________ _______________ a. This is when the genetic information in DNA is used to make proteins. b. There are 2 stages. 1. 39. _______________– make an RNA copy of a ...
... How the genes on DNA control protein production needed for a cell’s growth and function. Summary of how proteins are made: 38. _______________ _______________ a. This is when the genetic information in DNA is used to make proteins. b. There are 2 stages. 1. 39. _______________– make an RNA copy of a ...
Semiquantitative RT-PCR analysis
... 10 (Ultra5 homogenizer, Taitec, Saitama, Japan). For ChIP, the cell lysates were incubated with an agarose-conjugated anti-FLAG M2 antibody (Sigma), and the following steps were performed using EZ ChIP chromatin immunoprecipitation kit (Upstate, Charlottesville, VG). The isolated DNA was amplified b ...
... 10 (Ultra5 homogenizer, Taitec, Saitama, Japan). For ChIP, the cell lysates were incubated with an agarose-conjugated anti-FLAG M2 antibody (Sigma), and the following steps were performed using EZ ChIP chromatin immunoprecipitation kit (Upstate, Charlottesville, VG). The isolated DNA was amplified b ...
Measuring the Electron Transport Properties of DNA Molecules
... structures at such extreme nano-level resolutions is the lab’s use of negative-stiffness vibration isolation systems, developed by Minus K Technology, which produced the ultra-stable environment that the AFMs needed to execute this research. “Any lab site is subject to vibrations from machines, vibr ...
... structures at such extreme nano-level resolutions is the lab’s use of negative-stiffness vibration isolation systems, developed by Minus K Technology, which produced the ultra-stable environment that the AFMs needed to execute this research. “Any lab site is subject to vibrations from machines, vibr ...
Chapter 11
... DISCOVERING THE STRUCTURE OF DNA • Francis Crick and James Watson elaborated on the discoveries of Franklin and Chargaff and deduced that the structure of DNA was a double helix. • Two strands of DNA bound together by hydrogen bonds between the bases. • Because a purine of one strand binds to a pyr ...
... DISCOVERING THE STRUCTURE OF DNA • Francis Crick and James Watson elaborated on the discoveries of Franklin and Chargaff and deduced that the structure of DNA was a double helix. • Two strands of DNA bound together by hydrogen bonds between the bases. • Because a purine of one strand binds to a pyr ...
DNA, Transcription and Translation
... • The Building Blocks. • DNA is a macro-molecule, (large) which is made up of a series of chemical building blocks called nucleotides. Each nucleotide consists of 3 very different and separate components: • a phosphate group (P), • a five-carbon sugar, (S), (deoxyribose), • and one of four nitrogen- ...
... • The Building Blocks. • DNA is a macro-molecule, (large) which is made up of a series of chemical building blocks called nucleotides. Each nucleotide consists of 3 very different and separate components: • a phosphate group (P), • a five-carbon sugar, (S), (deoxyribose), • and one of four nitrogen- ...
DNA Technology and its Applications
... ▪ DNA fragments produced by the gel electrophoresis can be used in other technologies like PCR, cloning and DNA ...
... ▪ DNA fragments produced by the gel electrophoresis can be used in other technologies like PCR, cloning and DNA ...
English Version
... 1. General concepts of nucleic acids. (1) Nucleotides are the building blocks of nucleic acids. (2) Nucleotide is composed of base, pentose, and phosphate. (3) The 3’,5’phosphodiester bond links nucleotides, many nucleotides are linked by the 3’,5’ phosphodiester bond to form nucleic acids. (4) Nucl ...
... 1. General concepts of nucleic acids. (1) Nucleotides are the building blocks of nucleic acids. (2) Nucleotide is composed of base, pentose, and phosphate. (3) The 3’,5’phosphodiester bond links nucleotides, many nucleotides are linked by the 3’,5’ phosphodiester bond to form nucleic acids. (4) Nucl ...
Manipulating DNA
... Reading the DNA sequence: Obtain a single stranded piece of an organism’s DNA. As it replicates with bases labeled with color coded fluorescent dyes, the replication stops forming a fragment. After all of the DNA has replicated, tiny labeled fragments are left. The fragments are separated b ...
... Reading the DNA sequence: Obtain a single stranded piece of an organism’s DNA. As it replicates with bases labeled with color coded fluorescent dyes, the replication stops forming a fragment. After all of the DNA has replicated, tiny labeled fragments are left. The fragments are separated b ...
DNA and RNA - davis.k12.ut.us
... until all the bases are connected. Tape together the sugar-phosphate backbones of the nucleotides you added but do not tape the bases. You should now have two copies of your original molecule of DNA. 9. How does each new nucleotide chain compare to the one on which it was formed? 10. Why is DNA repl ...
... until all the bases are connected. Tape together the sugar-phosphate backbones of the nucleotides you added but do not tape the bases. You should now have two copies of your original molecule of DNA. 9. How does each new nucleotide chain compare to the one on which it was formed? 10. Why is DNA repl ...
Lab Techniques
... by a diseased cell to genes expressed by an healthy cell. • Other uses include- Testing for hereditary disease, Evolutionary history of species, Screening e.g.food supply • Applications to synthetic biology - identification of various parts in natural organisms, -?more? ...
... by a diseased cell to genes expressed by an healthy cell. • Other uses include- Testing for hereditary disease, Evolutionary history of species, Screening e.g.food supply • Applications to synthetic biology - identification of various parts in natural organisms, -?more? ...
PowerPoint Presentation - Creighton Chemistry Webserver
... • Methylation patterns are unique in different tissues • Active genes are less methylated than inactive genes • Methylated regions silence gene expression by interacting with proteins and preventing access to DNA ...
... • Methylation patterns are unique in different tissues • Active genes are less methylated than inactive genes • Methylated regions silence gene expression by interacting with proteins and preventing access to DNA ...
Notes - The University of Sydney
... phosphates and thus reduce the repulsive effect of the strong negative charges. Mg2+ ions while giving the best shielding are rarely used as these are necessary for reactions which hydrolyse the phosphodiester bond, thus breaking the sugar phosphate backbone (and you want to avoid this at all cost!) ...
... phosphates and thus reduce the repulsive effect of the strong negative charges. Mg2+ ions while giving the best shielding are rarely used as these are necessary for reactions which hydrolyse the phosphodiester bond, thus breaking the sugar phosphate backbone (and you want to avoid this at all cost!) ...
Restriction Enzymes
... They are proteins produced in a bacteria cell that cut DNA at a specific site. Also known as restriction endonucleases We can use these to manipulate DNA in the lab. ...
... They are proteins produced in a bacteria cell that cut DNA at a specific site. Also known as restriction endonucleases We can use these to manipulate DNA in the lab. ...
DNA
... – Growth hormones are used daily in many of the cattle, fish, and poultry that we eat. – We also use genetic engineering (DNA Fragment Injection) to gain strains of AIDS that we use to investigate the cure, further research into the human immune system, and many other medical ...
... – Growth hormones are used daily in many of the cattle, fish, and poultry that we eat. – We also use genetic engineering (DNA Fragment Injection) to gain strains of AIDS that we use to investigate the cure, further research into the human immune system, and many other medical ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""