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Poster
Poster

... from the Protein Data Bank and a visualization program called RasMol. T7 is virus that infects bacteria, but its RNA Polymerase is a very important molecule to scientists. Scientists can use T7 RNAP to create large amounts of a specific protein for their research or to study transcription in vitro. ...
CP Biology Chapter 8 Structure of DNA notes
CP Biology Chapter 8 Structure of DNA notes

... Transcription and replication share many similarities. For example, they both involve unwinding the DNA double helix, and both involve large enzymes called polymerases. But the end results of the two processes are very different. Replication makes a copy of DNA and transcription makes RNA molecules. ...
Nucleic Acids and Protein Synthesis
Nucleic Acids and Protein Synthesis

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Essential Biology Topic 4 File
Essential Biology Topic 4 File

... and these pieces can be added to the open plasmid and spliced together by ligase. The recombinant plasmids formed can be inserted into new host cells and cloned. ...
Introduction to Molecular Cell Biology (not tought by SK in 2010)
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... Nucleus: contains DNA, the source of the genetic code. It is responsible for storage of the code and its retrieval (e.g. production of mRNAs for the proteins required by the cell) Ribosomes: assemble proteins according to the instructions relayed by the messenger RNA Nucleolus – a dense spot p in th ...
GENETICS - St. Bonaventure University
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2012_Protein_Regionals_Exam

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... which traits acquired by parents could be passed on to successive generations. Although usually attributed to Jean Baptiste Lamarck, it was a commonly accepted method of inheritance in the late 19th and early 20th centuries. The rise of the “modern synthesis” of evolution rejects this mode of inheri ...
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... • EPO boosts production of red blood cells – Lance Armstrong used it. • Concern now that athletes may inject genes to make EPO into their cells • New test can scan for this gene using introns/exons! • A person’s own EPO gene has introns. • An inserted gene would likely lack those introns. So their a ...
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... Fertility-F-plasmid which contain tra-genes (transfer). They are capable of conjugation and help bacteria produce pili. Resistance- R-plasmids, which contain genes that can build a resistance against antibiotics or poisons. Col-plasmids, which contain genes that determine the production of bacterioc ...
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Regents Review 2.0 Living Environment PowerPoint Presentation
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... The flounder is a species of fish that can live in very cold water. The fish produces an “antifreeze” protein that prevents ice crystals from forming in its blood. The DNA for this protein has been identified. An enzyme is used to cut and remove this section of flounder DNA that is then spliced into ...
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... The flounder is a species of fish that can live in very cold water. The fish produces an “antifreeze” protein that prevents ice crystals from forming in its blood. The DNA for this protein has been identified. An enzyme is used to cut and remove this section of flounder DNA that is then spliced into ...
Bioinformatics and Personal Health/Intro computer lab
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... 3. Understand that genes often are members of gene families that may arise through gene duplication. 4. Be able to apply sequence analyses to identify mutations underlying specific phenotypes. 5. Understand how selection for specific phenotypes drove the Green Revolution. ...
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DNA and the Genome

DNA CLONING
DNA CLONING

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Microbial Genetics Part 2

... • She believed that the color variations were caused by “jumping genes” that would jump into or out of the middle of the chromosome. • Her theories were met with a great deal of criticism and weren’t accepted until almost 30 years later. • Transposons contain genes that enable the short segment of D ...
DNAandGeneticsEducDept
DNAandGeneticsEducDept

... couple who are planning to have a child but who suspect that there is a greater than normal risk of the child being affected by a genetic disorder ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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