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APBioReview
APBioReview

... plates full of ampicillin. They could only grow there if they contained the plasmid with the antibiotic gene (therefore the plasmid). If the sugar arabinose was present it turned on the gene which made the glow in the dark protein. Positive Control LB+ Negative Control LB/Amp- (+ or – the plasmid) ...
Questions - Vanier College
Questions - Vanier College

... a. Their muscles would be unable to contract. b. Their muscles would be unable to relax. c. Cell receptors would no longer be able to bind regulatory hormones. d. G-protein receptors would all be inactivated. 13. Pemphigus vulgaris is an autoimmune disorder in which antibodies are produced against t ...
AP Review
AP Review

... DNA strands line up in an antiparallel arrangement ...
Complete the following chart using your genetic code chart worksheet:
Complete the following chart using your genetic code chart worksheet:

... 5. Many chromosome mutations result when chromosomes fail to separate properly ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... decompressor are needed to see this picture. ...
DNA
DNA

... • The relationship between genes and their effects is complex • Despite the neatness of the genetic code, every gene cannot be simply lined to a single outcome • Some genes are expressed only at certain times or under some specific condition • Some traits result from the expression of multiple genes ...
Exam3-1406_Spring'06.doc
Exam3-1406_Spring'06.doc

... C) an animal cell undergoing cytokinesis D) a plant cell in metaphase E) a plant cell undergoing cytokinesis 50) Cytokinesis refers to the division of the A) cytoplasm. B) nucleus. C) mitochondria. D) centrioles. E) chromosomes. 51) Sister chromatids are A) duplicate chromosomes held together by a ...
Chapter 15
Chapter 15

... • Identified the deficiencies (ex: added arginine to the medium and this allowed the mutants to grow) • Results: each mutant had a defect in a single enzyme, caused by a mutation at a single site on one chromosome. • “One gene/one polypeptide”- genes produce their effects by specifying the structure ...
Guided Exploration- (RI3) Learning Goal Three: Explain how DNA is
Guided Exploration- (RI3) Learning Goal Three: Explain how DNA is

... DNA is the directions to build our bodies. The only problem is, DNA is locked inside the nucleus of a cell and can’t get out. To solve this problem, copies of the DNA are made in a form called mRNA. The process of making mRNA from DNA is called transcription. After transcription, the mRNA copies lea ...
Bacteria and Archaea Chapter 27A:
Bacteria and Archaea Chapter 27A:

... 0.3 µm ...
Cell cycle and Reproduction - River Dell Regional School District
Cell cycle and Reproduction - River Dell Regional School District

... Most of the time, the DNA in each chromosome is wound around proteins called histones These DNA-histone spools are further folded into coils Another layer of folding occurs as the coiled strand folds into loops, which are then attached to protein scaffolding, so that the chromosome is 1,000 times sh ...
Unit 5 Molecular Genetics Objectives
Unit 5 Molecular Genetics Objectives

... 3 Inducers and repressors are small molecules that interact with regulatory proteins and/or regulatory sequences. 4 Regulatory proteins inhibit gene expression by binding to DNA and blocking transcription (negative control). 5 Regulatory proteins stimulate gene expression by binding to DNA and stimu ...
Syllabus Notes - Southwest High School
Syllabus Notes - Southwest High School

... – They are catalysts. (They speed up reactions that would normally happen anyway.) – They do not use energy to work. – They do not get used up. They do not change – Substrates are what the enzymes work on. ...
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation
BIO208 Bacterial Genetics Worksheet 1 1. . Fill in: Transformation

... transformed with a plasmid that contains a wild type (normal) promoter, amp resistance gene, and origin of replication. Can the cell utilize lactose? (i.e. is the operon inducible?) Why or why not? The cell cannot utilize lactose because the promoter is defective. The addition of a normal promoter o ...
Life on Mars
Life on Mars

... programme allows you to input a DNA sequence of interest and BLAST will identify regions of similarity between sequences that are stored on its DNA database. The program compares nucleotide (or protein) sequences to sequence databases and calculates the statistical significance of matches. The resul ...
Organization of the eukaryotic genomes
Organization of the eukaryotic genomes

The chemical components in DNA
The chemical components in DNA

Instructor`s Answer Key
Instructor`s Answer Key

... saturated fat intake should not exceed 10% of a person’s total fat intake because they may contribute to high blood cholesterol – a significant risk factor in heart disease and stroke. By contrast, certain oils such as the omega-3 rich fish oils seem to have a protective effect in heart disease. ...
Chapter 14 Constant Allele Frequencies
Chapter 14 Constant Allele Frequencies

... C. STRs are nonuniformly distributed. D. restrictive enzymes cannot be used to cut short DNA molecules. 25. Principles of population genetics must be applied to determine identity based on DNA profiling because A. VNTRs are not found in all populations. B. individuals are their own populations. C. n ...
Chapter 14 Constant Allele Frequencies
Chapter 14 Constant Allele Frequencies

... C. STRs are nonuniformly distributed. D. restrictive enzymes cannot be used to cut short DNA molecules. 25. Principles of population genetics must be applied to determine identity based on DNA profiling because A. VNTRs are not found in all populations. B. individuals are their own populations. C. n ...
Protein sequence database
Protein sequence database

... This is the NIH genetic sequence database, an annotated collection of all publicly available DNA sequences. Genbank is part of the international nucleotide sequence database collaboration, which comprises the DNA databank of Japan (DDBJ), the European molecular biology laboratory (EMBL), and Genbank ...
Genome Editing Slides
Genome Editing Slides

... • Discovered as what prokaryotes have as an immune system • Pallindromic Repeats of 20-40 bases, separated by short sequences that turn out to be leftover from bacterial viruses that had previously infected the cell – Pallindromic DNA, when transcribed make RNA’s that can base pair with themselves t ...
5. Nucleic Acids-Structure, Central Dogma – Bio 20
5. Nucleic Acids-Structure, Central Dogma – Bio 20

Mutations Website Assignment - Mercer Island School District
Mutations Website Assignment - Mercer Island School District

... 8. Being a carrier of the sickle cell anemia allele has a positive effect (which explains why this genetic disorder is most common among people who come from tropical areas have certain strains of mosquitoes.) Explain what this positive effect is. ...
File
File

... prokaryotes have a circular DNA- means no problem with ends eukaryotes have linear DNA - problem with replicating ends (telemers); ends have repeat sequencesenzyme telemerase can extend ends up to an early age; after that every time DNA replicates, it is shortened. prokaryote - one origin of replica ...
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Molecular cloning



Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.
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