Honors Biology
... 1. Summarize how restriction enzymes cut DNA. 2. Explain how restriction maps show the lengths of DNA fragments. 3. Describe the role of polymerases in copying DNA segments. 4. Outline the PCR process and explain why it is used. 5. Describe what a DNA fingerprint represents. 6. Summarize how DNA fin ...
... 1. Summarize how restriction enzymes cut DNA. 2. Explain how restriction maps show the lengths of DNA fragments. 3. Describe the role of polymerases in copying DNA segments. 4. Outline the PCR process and explain why it is used. 5. Describe what a DNA fingerprint represents. 6. Summarize how DNA fin ...
Student Note Packet
... – trisomy: individual has three copies (2 + 1) - extra gene products lead to unbalance = abnormality/death • Down’s syndrome = trisomy 21 (3 copies of chromosome 21) polyploidy: more than diploid set of chromosomes - common in flowering plants (extremely rare in animals) - must be same number of eac ...
... – trisomy: individual has three copies (2 + 1) - extra gene products lead to unbalance = abnormality/death • Down’s syndrome = trisomy 21 (3 copies of chromosome 21) polyploidy: more than diploid set of chromosomes - common in flowering plants (extremely rare in animals) - must be same number of eac ...
0101BWhat characterizes a prokaryotic cell
... __89) Which of the statements regarding enzymes is false? a) Enzymes are proteins that function as catalysts. b) Enzymes display specificity for certain molecules to which they attach. c) Enzymes provide activation energy for the reactions they catalyze. d) The activity of enzymes can be regulated. ...
... __89) Which of the statements regarding enzymes is false? a) Enzymes are proteins that function as catalysts. b) Enzymes display specificity for certain molecules to which they attach. c) Enzymes provide activation energy for the reactions they catalyze. d) The activity of enzymes can be regulated. ...
From Gene to Protein—Transcription and Translation
... the figure on page 4 of your biology background and instructions handout.) Student answers may vary. Student should included: the idea that during protein synthesis the DNA “language” has to be converted in RNA “language” in order for the information it contains to be understood and carried out. Thi ...
... the figure on page 4 of your biology background and instructions handout.) Student answers may vary. Student should included: the idea that during protein synthesis the DNA “language” has to be converted in RNA “language” in order for the information it contains to be understood and carried out. Thi ...
13-2 Manipulating DNA
... • Transgenic bacteria produce important substances useful for health and industry. ...
... • Transgenic bacteria produce important substances useful for health and industry. ...
Genomics
... Real time PCR +s and -s • Advantages – Relatively easy – Very sensitive – Gold standard of measuring gene expression quantitatively – Often used to confirm microarray results ...
... Real time PCR +s and -s • Advantages – Relatively easy – Very sensitive – Gold standard of measuring gene expression quantitatively – Often used to confirm microarray results ...
File
... Steps in processing 1. Add the cap – cells have different types of caps (5 cap is made up of modified Guanine) 2. 3 tail 3. Splicing is the removal of the introns Eukaryotic organisms – interrupted gene has two parts 1. Exons – DNA sequence which is transcribed into RNA to be transferred into protei ...
... Steps in processing 1. Add the cap – cells have different types of caps (5 cap is made up of modified Guanine) 2. 3 tail 3. Splicing is the removal of the introns Eukaryotic organisms – interrupted gene has two parts 1. Exons – DNA sequence which is transcribed into RNA to be transferred into protei ...
Anatomy and Physiology BIO 137
... • It is often of interest in forensic science to identify individuals genetically. In these cases, one is interested in looking at variable regions of the genome as opposed to highly-conserved genes. • PCR can be used to amplify highly variable regions of the human genome. These regions contain runs ...
... • It is often of interest in forensic science to identify individuals genetically. In these cases, one is interested in looking at variable regions of the genome as opposed to highly-conserved genes. • PCR can be used to amplify highly variable regions of the human genome. These regions contain runs ...
Molecular Biology BIO 250
... Know what linkage is. How is the behavior of linked genes during meiosis different from genes that Mendel studied? Which one of Mendel’s laws does not apply when two genes are linked? How is genetic distance between two genes located on the same chromosome calculated using linkage? Who discovere ...
... Know what linkage is. How is the behavior of linked genes during meiosis different from genes that Mendel studied? Which one of Mendel’s laws does not apply when two genes are linked? How is genetic distance between two genes located on the same chromosome calculated using linkage? Who discovere ...
Midterm #1 Study Guide
... What are some examples of how DNA is used in pharmaceutical products and medicine? What are genetically modified organisms? What are transgenic organisms? What is a clone? What is reproductive cloning? What is somatic cell nuclear transfer (SCNT)? What are stem cells? How does gene therapy work? Wh ...
... What are some examples of how DNA is used in pharmaceutical products and medicine? What are genetically modified organisms? What are transgenic organisms? What is a clone? What is reproductive cloning? What is somatic cell nuclear transfer (SCNT)? What are stem cells? How does gene therapy work? Wh ...
B left E
... 16. Can DNA Polymerase I fragment remove the RNA primer from Okazaki fragments? A. Yes, it has the ability to ligate the DNA nicks together B. No, because it is not a very processive enzyme C. No, because it lacks the 5’-> 3’ exonuclease D. No, because it lacks the 3’-> 5’ exonuclease E. The RNA pri ...
... 16. Can DNA Polymerase I fragment remove the RNA primer from Okazaki fragments? A. Yes, it has the ability to ligate the DNA nicks together B. No, because it is not a very processive enzyme C. No, because it lacks the 5’-> 3’ exonuclease D. No, because it lacks the 3’-> 5’ exonuclease E. The RNA pri ...
Instructions for Biochemistry
... life’s ultimate building blocks, called amino acids. The 20 different amino acids provide 20 diverse building blocks to make proteins. A gene, made of DNA, is chiefly a code to make the proteins that are critical in almost every function of our cells. After the DNA is transcribed into RNA, cellular ...
... life’s ultimate building blocks, called amino acids. The 20 different amino acids provide 20 diverse building blocks to make proteins. A gene, made of DNA, is chiefly a code to make the proteins that are critical in almost every function of our cells. After the DNA is transcribed into RNA, cellular ...
Chapter 8
... • This makes most scientist believe that all living organisms gave rise from a common ancestor. • It also means scientists can use a gene from one organism in different organisms. • So , how do we get a protein made from the instructions mRNA carries to the ribosomes? • This process is called transl ...
... • This makes most scientist believe that all living organisms gave rise from a common ancestor. • It also means scientists can use a gene from one organism in different organisms. • So , how do we get a protein made from the instructions mRNA carries to the ribosomes? • This process is called transl ...
chapter 3
... complex DNA template in a simple enzymatic reaction. This method utilizes a DNA polymerase and two oligonucleotide primers to synthesize a specific DNA from a single stranded template sequence. The oligonucleotides typically have different sequences and are complementary to sequences that lie on opp ...
... complex DNA template in a simple enzymatic reaction. This method utilizes a DNA polymerase and two oligonucleotide primers to synthesize a specific DNA from a single stranded template sequence. The oligonucleotides typically have different sequences and are complementary to sequences that lie on opp ...
20_Lecture_Presentation_PC
... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
... • The remarkable ability of bacteria to express some eukaryotic proteins underscores the shared evolutionary ancestry of living species • For example, Pax-6 is a gene that directs formation of a vertebrate eye; the same gene in flies directs the formation of an insect eye (which is quite different f ...
Heredity Study Guide
... Selective breeding: specific traits are selected in the parents in order to ensure they are passed to the offspring & the genes are not actually altered 20. List some positive uses for selective breeding. The traits can easily be predicted. You can produce offspring that can serve a specific purpose ...
... Selective breeding: specific traits are selected in the parents in order to ensure they are passed to the offspring & the genes are not actually altered 20. List some positive uses for selective breeding. The traits can easily be predicted. You can produce offspring that can serve a specific purpose ...
Heredity Study Guide Answers
... Selective breeding: specific traits are selected in the parents in order to ensure they are passed to the offspring & the genes are not actually altered 20. List some positive uses for selective breeding. The traits can easily be predicted. You can produce offspring that can serve a specific purpose ...
... Selective breeding: specific traits are selected in the parents in order to ensure they are passed to the offspring & the genes are not actually altered 20. List some positive uses for selective breeding. The traits can easily be predicted. You can produce offspring that can serve a specific purpose ...
7. According to Dr. Malcolm (guy in black leather jacket), “Dinosaurs
... Remember when we talked about traditional plant breeding? It mimics what can happen on its own in nature. Well, this natural cross-breeding of plants is another way that genetic engineering could be a very bad thing for the environment. One of the most popular methods of genetically engineering plan ...
... Remember when we talked about traditional plant breeding? It mimics what can happen on its own in nature. Well, this natural cross-breeding of plants is another way that genetic engineering could be a very bad thing for the environment. One of the most popular methods of genetically engineering plan ...
DNA
... Minute amounts of DNA template may be used from as little as a single cell. DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR r ...
... Minute amounts of DNA template may be used from as little as a single cell. DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR r ...
DNA and RNA Chapter 12
... Mendel/flower images from: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC.html Blood cell by Riedell ...
... Mendel/flower images from: http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookTOC.html Blood cell by Riedell ...
Document
... • Extract and amplify DNA from different food samples • Perform genuine diagnostic procedures • Use PCR and electrophoresis to find GMO foods • Sufficient materials for 8 student workstations • Complete the activity in three 45 minute lab sessions ...
... • Extract and amplify DNA from different food samples • Perform genuine diagnostic procedures • Use PCR and electrophoresis to find GMO foods • Sufficient materials for 8 student workstations • Complete the activity in three 45 minute lab sessions ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.