UltraClean 15 DNA Purification Kit
... We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be used. It is not necessary to use low melt agarose for good recoveries with this kit. If the ULTRA BIND Dries Out It ...
... We recommend pH 7.5 - 7.8 for running all TAE gels and pH 8.0 - 8.3 for TBE gels. Agarose gel concentrations up to 4% are compatible with this kit. All agarose brands and types can be used. It is not necessary to use low melt agarose for good recoveries with this kit. If the ULTRA BIND Dries Out It ...
ppt - eweb.furman.edu
... 1. Prokaryotes 2. Eukaryotes – usually many linear chromosomes, highly condensed with histone proteins into several levels of structure. To read a gene, the chromosome must be diffuse (uncondensed) in that region. Even when condensed, these ‘euchromatic’ coding regions are less condensed and more li ...
... 1. Prokaryotes 2. Eukaryotes – usually many linear chromosomes, highly condensed with histone proteins into several levels of structure. To read a gene, the chromosome must be diffuse (uncondensed) in that region. Even when condensed, these ‘euchromatic’ coding regions are less condensed and more li ...
Molecular Cell Biology - Biomedical Informatics
... Figure 9-30. Structure of the nucleosome. (a) Ribbon diagram of the nucleosome shown face-on (left) and from the side (right). One DNA strand is shown in green and the other in brown. H2A is yellow; H2B, red; H3, blue; H4, green. (b) Space-filling model shown from the side. DNA is shown in white; hi ...
... Figure 9-30. Structure of the nucleosome. (a) Ribbon diagram of the nucleosome shown face-on (left) and from the side (right). One DNA strand is shown in green and the other in brown. H2A is yellow; H2B, red; H3, blue; H4, green. (b) Space-filling model shown from the side. DNA is shown in white; hi ...
Antisense Oligonucleotides: Strategies and Applications
... modification [5]. They replaced one of the non-bridging oxygen atoms in the phosphate backbone with a sulfur atom (Figure 2A). Called a phosphorothioate, this modification did achieve the goal of nuclease resistance as measured by an increased half-life for a phosphorothioated oligonucleotide of up ...
... modification [5]. They replaced one of the non-bridging oxygen atoms in the phosphate backbone with a sulfur atom (Figure 2A). Called a phosphorothioate, this modification did achieve the goal of nuclease resistance as measured by an increased half-life for a phosphorothioated oligonucleotide of up ...
MagJET Plasmid DNA Kit - Thermo Fisher Scientific
... technology for nucleic acid purification. The whole nucleic acid isolation process combines simple steps of sample lysis, DNA binding to the magnetic beads, washing and elution. Pelleted bacterial cells are resuspended and subjected to SDS/alkaline lysis to liberate plasmid DNA. The resulting lysate ...
... technology for nucleic acid purification. The whole nucleic acid isolation process combines simple steps of sample lysis, DNA binding to the magnetic beads, washing and elution. Pelleted bacterial cells are resuspended and subjected to SDS/alkaline lysis to liberate plasmid DNA. The resulting lysate ...
Gene Section ERCC3 (Excision repair cross-complementing 3)
... allowing promoter clearance. In the NER process TFIIH causes unwinding of the lesion-containing region that has been localized by XPC-HR23B and XPA-RPA, enabling the accumulation of NER proteins around the damaged site. Among the Xeroderma pigmentosum (XP) patients, XPB patients are extremely rare ( ...
... allowing promoter clearance. In the NER process TFIIH causes unwinding of the lesion-containing region that has been localized by XPC-HR23B and XPA-RPA, enabling the accumulation of NER proteins around the damaged site. Among the Xeroderma pigmentosum (XP) patients, XPB patients are extremely rare ( ...
Chpt3_Isolating_analyzing_genes.doc
... Recombinant DNA, Polymerase Chain Reaction and Applications to Eukaryotic Gene Structure and Function The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned ...
... Recombinant DNA, Polymerase Chain Reaction and Applications to Eukaryotic Gene Structure and Function The first two chapters covered many important aspects of genes, such as how they function in inheritance, how they code for protein (in general terms) and their chemical nature. All this was learned ...
Electronic Supplemental Information (ESI) for Quantifying mRNA
... It was necessary to perform PCR in a 384-well thermocycler, but a fluorescent plate reader was used to collect multiplex fluorescent data. Initially, a typhoon 1410 flatbed scanner was used to collect data at PCR cycles 15, 20, 25, and 30. However, a Tecan 200 plate reader was identified as a faster ...
... It was necessary to perform PCR in a 384-well thermocycler, but a fluorescent plate reader was used to collect multiplex fluorescent data. Initially, a typhoon 1410 flatbed scanner was used to collect data at PCR cycles 15, 20, 25, and 30. However, a Tecan 200 plate reader was identified as a faster ...
No Slide Title
... A common mechanism of DNA bending by minor groove-binding proteins is the insertion of protein side chains between basepair steps, exemplified in TBP/DNA complexes. At the first and last basepair steps of the TATA box, TBP kinks the DNA by inserting pairs of Phe side chains between the steps, and pl ...
... A common mechanism of DNA bending by minor groove-binding proteins is the insertion of protein side chains between basepair steps, exemplified in TBP/DNA complexes. At the first and last basepair steps of the TATA box, TBP kinks the DNA by inserting pairs of Phe side chains between the steps, and pl ...
Solid state NMR assignment of a whole virus particle
... The helix is assumed to have a kink in the center, or to be gently curved the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alter ...
... The helix is assumed to have a kink in the center, or to be gently curved the N-terminus can be a loop (1PJF,1QL1,4IFM) or helical (1PFI, 1IFM) and probably depends on the solvent (surface exposed) The locations of the sidechains are unresolved in the x-ray data and are partially obtained from alter ...
... of prokaryote cells is considered as they triggered the beginning of planet’s life. When three of prokaryote cells gathered, they formed eukaryotic cell. For instance, recently, escherichia coli bacteria is the representative organism included in prokaryotic organisms. The endosymbiotic theory insis ...
Southern Blot Analysis of Plasmids pRIT4501 and - RIT
... Analysis of Plasmids pRIT4501 and pRIT4502 It is often desirable to screen nucleic acids to determine whether or not they carry specific sequences. Typically, this is done by making the nucleic acid in question single-stranded, fixing it to a solid support, and challenging it with a small, labeled, ...
... Analysis of Plasmids pRIT4501 and pRIT4502 It is often desirable to screen nucleic acids to determine whether or not they carry specific sequences. Typically, this is done by making the nucleic acid in question single-stranded, fixing it to a solid support, and challenging it with a small, labeled, ...
PowerPoint 14 – Enzymes
... Each enzyme is specially built to either bring specific substances together making a new substance, or to break substances apart ...
... Each enzyme is specially built to either bring specific substances together making a new substance, or to break substances apart ...
![Trends in Genetics 9:375. [pdf reprint 109 kb]](http://s1.studyres.com/store/data/017557884_1-28a68d1c60cf8baa3f06095d10e78800-300x300.png)
Trends in Genetics 9:375. [pdf reprint 109 kb]
... lactose agar can be used instead of agar that contains X-Gal-IPTG. However, this method is not equally suitable for use with all plasmid vectors that carry this marker. Moreover, the results can vary depending on the source of the MacConkey medium used. In addition to the pUC9, pBluescript and pGEM4 ...
... lactose agar can be used instead of agar that contains X-Gal-IPTG. However, this method is not equally suitable for use with all plasmid vectors that carry this marker. Moreover, the results can vary depending on the source of the MacConkey medium used. In addition to the pUC9, pBluescript and pGEM4 ...
The effect of human serum DNAases on the ability to detect
... without exposure to cefotaxime. After another 90 min (a total of 2 h after exposure to cefotaxime), bacterial ¯uorescence was measured by ¯ow cytometry (Coulter Elite ESP ¯ow cytometer) with a UV-enhanced argon laser for excitation and a 405-nm bandpass ®lter set for emission. Approximately 5000 eve ...
... without exposure to cefotaxime. After another 90 min (a total of 2 h after exposure to cefotaxime), bacterial ¯uorescence was measured by ¯ow cytometry (Coulter Elite ESP ¯ow cytometer) with a UV-enhanced argon laser for excitation and a 405-nm bandpass ®lter set for emission. Approximately 5000 eve ...
Protein expression, purification, and molecular cloning
... completely dissolved. DNA yields will significantly decrease when the pH > 8.0. If the color of the mixture becomes orange or red, add 5 µL 5M sodium acetate (pH 5.2) to bring the pH down. After this adjustment, the color of the Gel/Binding Buffer mixture should be light yellow. Insert a HiBind® DNA ...
... completely dissolved. DNA yields will significantly decrease when the pH > 8.0. If the color of the mixture becomes orange or red, add 5 µL 5M sodium acetate (pH 5.2) to bring the pH down. After this adjustment, the color of the Gel/Binding Buffer mixture should be light yellow. Insert a HiBind® DNA ...
Thermo Scientific TurboFect Transfection Reagent
... 1. In each well, seed cells in 200 μL of growth medium 24 hours prior to transfection at a density that will give a confluency of 70-90% the day of transfection. Note: The recommended confluency for adherent cells on the day of transfection is 70-90%. Suspension cells should be in logarithmic growt ...
... 1. In each well, seed cells in 200 μL of growth medium 24 hours prior to transfection at a density that will give a confluency of 70-90% the day of transfection. Note: The recommended confluency for adherent cells on the day of transfection is 70-90%. Suspension cells should be in logarithmic growt ...
C8 Challenge
... The process in which bacterial DNA is transferred from a donor cell to a recipient cell inside a bacteriophage is called a. b. c. d. ...
... The process in which bacterial DNA is transferred from a donor cell to a recipient cell inside a bacteriophage is called a. b. c. d. ...
Mutations - GK-12 Program at the University of Houston
... to the proteins that are encoded by the DNA which can lead to a loss of functionality for those proteins. Substitutions, or point mutations, are much more subtle and may have three possible effects. Figure 3 shows how some point mutations may lead to common disorders. 1. Silent – the nucleotide is r ...
... to the proteins that are encoded by the DNA which can lead to a loss of functionality for those proteins. Substitutions, or point mutations, are much more subtle and may have three possible effects. Figure 3 shows how some point mutations may lead to common disorders. 1. Silent – the nucleotide is r ...
Dr. Apr. Dieter Deforce
... 2.B.5. Special precautions to be taken in performing PCR for diagnostic purposes: Contamination is one of the major reasons for misinterpretation of results with PCR and occurs when exogenous DNA or RNA, proteases, nucleases and various inhibitors of Taq polymerase are introduced into the reaction m ...
... 2.B.5. Special precautions to be taken in performing PCR for diagnostic purposes: Contamination is one of the major reasons for misinterpretation of results with PCR and occurs when exogenous DNA or RNA, proteases, nucleases and various inhibitors of Taq polymerase are introduced into the reaction m ...
Molecular cloning, characterization and expression of
... manufacturer’s instruction. Developing seeds (30 DAF) (50-100 mg) were sampled, grounded in liquid N2 and homogenized in 1 ml of TRI-reagent. The homogenate was stored at room temperature for 5 min to permit dissociation of nucleoprotein complexes. Chloroform (0.2 ml) was added to the homogenate and ...
... manufacturer’s instruction. Developing seeds (30 DAF) (50-100 mg) were sampled, grounded in liquid N2 and homogenized in 1 ml of TRI-reagent. The homogenate was stored at room temperature for 5 min to permit dissociation of nucleoprotein complexes. Chloroform (0.2 ml) was added to the homogenate and ...
DNA shuffling by random fragmentation and reassembly: In
... 3 s per cycle. Gel analysis showed that most of the PCR product was >>20 kb. After digestion with a unique enzyme (EcoO109), most of the product consisted of a single band of the expected size, which was gel purified, ligated, transformed, and plated on LB/ampicillin/X-Gal plates as described above. ...
... 3 s per cycle. Gel analysis showed that most of the PCR product was >>20 kb. After digestion with a unique enzyme (EcoO109), most of the product consisted of a single band of the expected size, which was gel purified, ligated, transformed, and plated on LB/ampicillin/X-Gal plates as described above. ...
mRNA Codon
... Proteins are vital to living organisms. They are involved in chemical reactions, oxygen transport, muscle contraction, sensory perception, blood clotting, and many other activities. The great variety of roles requires equal variety in the structure of protein molecules. This variety is achieved by m ...
... Proteins are vital to living organisms. They are involved in chemical reactions, oxygen transport, muscle contraction, sensory perception, blood clotting, and many other activities. The great variety of roles requires equal variety in the structure of protein molecules. This variety is achieved by m ...
51 - Lab Times
... fence of prokaryotes against foreign DNA, Restriction enzymes are epitomes of restriction enzymes are found in almost any specificity. But even restriction enzymes bacteria and archaea. The restriction enwith an almost perfect sequence specificzyme database REBASE, run by restriction ity take it eas ...
... fence of prokaryotes against foreign DNA, Restriction enzymes are epitomes of restriction enzymes are found in almost any specificity. But even restriction enzymes bacteria and archaea. The restriction enwith an almost perfect sequence specificzyme database REBASE, run by restriction ity take it eas ...
Molecular cloning
Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically modified microorganisms (GMO). This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single recombinant DNA molecule. This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the original recombinant molecule. Thus, both the resulting bacterial population, and the recombinant DNA molecule, are commonly referred to as ""clones"". Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them.