Revised 2015 15.2 PowerPoint
... Transgenic animals can help create new medicines & produce valuable products. One of the first uses of transgenesis was to make the E. Coli bacteria produce human insulin, which could then be gathered cheaply, rather than having to be harvested from more expensive animals like pigs. A more contempor ...
... Transgenic animals can help create new medicines & produce valuable products. One of the first uses of transgenesis was to make the E. Coli bacteria produce human insulin, which could then be gathered cheaply, rather than having to be harvested from more expensive animals like pigs. A more contempor ...
Restriction Enzyme Worksheet
... Background: DNA fingerprinting is made possible in part by special enzymes that cut DNA. These enzymes are called restriction enzymes. Restriction enzymes are proteins that bacteria use to cut up DNA that doesn’t belong to them. If a bacterium senses that a virus is trying to invade, or a different ...
... Background: DNA fingerprinting is made possible in part by special enzymes that cut DNA. These enzymes are called restriction enzymes. Restriction enzymes are proteins that bacteria use to cut up DNA that doesn’t belong to them. If a bacterium senses that a virus is trying to invade, or a different ...
tools of genetic engineering
... a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. restriction enzyme An endonuclease that catalyzes double-strand cleavage of DNA containing a specific sequence. ...
... a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. restriction enzyme An endonuclease that catalyzes double-strand cleavage of DNA containing a specific sequence. ...
Name: Period: ______
... So far, we’ve learned that DNA is the genetic material that organisms inherit from their parents, but have you thought about what exactly is encoded for by this DNA? How do our cells use DNA as a set of instructions for life? How is the information in our DNA and genes used by our bodies? And what h ...
... So far, we’ve learned that DNA is the genetic material that organisms inherit from their parents, but have you thought about what exactly is encoded for by this DNA? How do our cells use DNA as a set of instructions for life? How is the information in our DNA and genes used by our bodies? And what h ...
DNA - EPFL
... DNA Replication, ctd • DNA synthesis occurs in the chemical direction 5’3’ • Nucleic acid chains are assembled from 5’ triphosphates of deoxyribonucleosides (the triphosphates supply energy) • DNA polymerases are enzymes that copy (replicate) DNA • DNA polymerases require a short preexisting DNA s ...
... DNA Replication, ctd • DNA synthesis occurs in the chemical direction 5’3’ • Nucleic acid chains are assembled from 5’ triphosphates of deoxyribonucleosides (the triphosphates supply energy) • DNA polymerases are enzymes that copy (replicate) DNA • DNA polymerases require a short preexisting DNA s ...
DNA replication
... - Thus when each strand of the double stranded parental DNA molecules separates from its complement during replication, each ...
... - Thus when each strand of the double stranded parental DNA molecules separates from its complement during replication, each ...
Solutions to 7.014 Problem Set 7
... 2) Ligate the mouse DNA into the vector. 3) Transform ampicillin and kanamycin sensitive bacteria with this ligation mixture. 4) Plate the transformed bacteria on media containing ampicillin. This selects for cells that are carrying a plasmid. ii) Once you have the mouse genomic library in E. Coli, ...
... 2) Ligate the mouse DNA into the vector. 3) Transform ampicillin and kanamycin sensitive bacteria with this ligation mixture. 4) Plate the transformed bacteria on media containing ampicillin. This selects for cells that are carrying a plasmid. ii) Once you have the mouse genomic library in E. Coli, ...
Restriction enzymes
... Gene cloning and genetic engineering were made possible by the discovery of restriction enzymes that cut DNA molecules at specific locations. ...
... Gene cloning and genetic engineering were made possible by the discovery of restriction enzymes that cut DNA molecules at specific locations. ...
Biosafety and recombinant DNA technology
... • Biological expression systems consist of vectors and host cells. A number of criteria must be satisfied to make them effective and safe to use. Plasmid pUC18. • Frequently used as a cloning vector in combination with Escherichia coli K12 cells, the pUC18 plasmid has been entirely sequenced. • All ...
... • Biological expression systems consist of vectors and host cells. A number of criteria must be satisfied to make them effective and safe to use. Plasmid pUC18. • Frequently used as a cloning vector in combination with Escherichia coli K12 cells, the pUC18 plasmid has been entirely sequenced. • All ...
DNA Challenge DNA Challenge
... A. Performs transcription and record the mRNA letters that will be created. B. After mRNA processing, list the final mRNA. f l h f l ...
... A. Performs transcription and record the mRNA letters that will be created. B. After mRNA processing, list the final mRNA. f l h f l ...
Microbial nutrition
... temp. between( 15-20) c° such as Vibrio. b- Mesophiles:- M.O grow optimally around (20-45)c° such as all human pathogen for ex. Staphylococcus aureus , Enterococcus. c- Thermophiles:- M.O they can grow at temp of 55c° or higher for ex. ...
... temp. between( 15-20) c° such as Vibrio. b- Mesophiles:- M.O grow optimally around (20-45)c° such as all human pathogen for ex. Staphylococcus aureus , Enterococcus. c- Thermophiles:- M.O they can grow at temp of 55c° or higher for ex. ...
Biology 1 Exam III Summer2005(ch8-9-10-11).doc
... d) All of the above e) None of the above. 13) Gametes are examples of: a) haploid cells. b) somatic cells. c) diploid cells. d) the products of mitotic division. e) things your parents don’t want to talk about 14) The sequence of nitrogen-containing bases on one strand of DNA could determine the A) ...
... d) All of the above e) None of the above. 13) Gametes are examples of: a) haploid cells. b) somatic cells. c) diploid cells. d) the products of mitotic division. e) things your parents don’t want to talk about 14) The sequence of nitrogen-containing bases on one strand of DNA could determine the A) ...
For the Tutorial Programme in Proteomics High
... Figure 1B). cDNA synthesized from gene transcripts can be used as templates for PCR reactions and allow the cloning of the transcript into vectors. Restriction enzymes type II and ligases. These two sets of enzymes have complementary activity, restriction enzymes work as “scissors” capable of identi ...
... Figure 1B). cDNA synthesized from gene transcripts can be used as templates for PCR reactions and allow the cloning of the transcript into vectors. Restriction enzymes type II and ligases. These two sets of enzymes have complementary activity, restriction enzymes work as “scissors” capable of identi ...
Microbial nutrition
... temp. between( 15-20) c° such as Vibrio. b- Mesophiles:- M.O grow optimally around (20-45)c° such as all human pathogen for ex. Staphylococcus aureus , Enterococcus. c- Thermophiles:- M.O they can grow at temp of 55c° or higher for ex. ...
... temp. between( 15-20) c° such as Vibrio. b- Mesophiles:- M.O grow optimally around (20-45)c° such as all human pathogen for ex. Staphylococcus aureus , Enterococcus. c- Thermophiles:- M.O they can grow at temp of 55c° or higher for ex. ...
Miocene DNA sequences
... fragment will be depurinated after about 5 000 years. At higher or lower pH the rate would be faster, whereas at 5°C the reaction would be two to four times slower. As baseless sites rapidly lead to strand breaks [S], it seems that the probability of any 800.bp DNA molecule surviving 16 million year ...
... fragment will be depurinated after about 5 000 years. At higher or lower pH the rate would be faster, whereas at 5°C the reaction would be two to four times slower. As baseless sites rapidly lead to strand breaks [S], it seems that the probability of any 800.bp DNA molecule surviving 16 million year ...
Unit 8.3: Biotechnology
... The polymerase chain reaction (PCR) makes many copies of a gene or other DNA segment. This might be done in order to make large quantities of a gene for genetic testing. PCR involves three steps: denaturing, annealing, and extension. The three steps are illustrated in Figure below. They are repeated ...
... The polymerase chain reaction (PCR) makes many copies of a gene or other DNA segment. This might be done in order to make large quantities of a gene for genetic testing. PCR involves three steps: denaturing, annealing, and extension. The three steps are illustrated in Figure below. They are repeated ...
Biology Chapters 8 and 9 Test Review
... formed. In plant cells, the cell plate, formed by cellulose, breaks the cell in half physically. In animal cells, the cleavage furrow pinches the cell into two cells. Cytokinesis—divides the entire cell into two pieces after telophase. o CDK, or cyclin dependent kinase, adds ATP to other substance ...
... formed. In plant cells, the cell plate, formed by cellulose, breaks the cell in half physically. In animal cells, the cleavage furrow pinches the cell into two cells. Cytokinesis—divides the entire cell into two pieces after telophase. o CDK, or cyclin dependent kinase, adds ATP to other substance ...
PDF
... to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach described in this report, a tingle transformant, harbouring pSA3, when grown nonselectively wil ...
... to integrate D N A into the L. plantarum genome. The major advantage of this system, compared to the use of suicide vectors [3] is that the latter requires a high transformation frequency. In the approach described in this report, a tingle transformant, harbouring pSA3, when grown nonselectively wil ...
Applications of Molecular Biology in Archaeology
... scientists did not expect long-term DNA-preservation in ancient remains because degradation results shortly after individual’s death. http://www.pbs.org/wnet/pharaohs/secrets3.html ...
... scientists did not expect long-term DNA-preservation in ancient remains because degradation results shortly after individual’s death. http://www.pbs.org/wnet/pharaohs/secrets3.html ...
Transformation (genetics)
In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occurs naturally in some species of bacteria, but it can also be effected by artificial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density.Transformation is one of three processes by which exogenous genetic material may be introduced into a bacterial cell, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium).""Transformation"" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because ""transformation"" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material. Introduction of foreign DNA into eukaryotic cells is often called ""transfection"".