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Environmental Microbiology
Environmental Microbiology

... plants (Barbieri et al., 1986). However, it has not been possible so far to construct completely IAA-negative mutants. A strain with a knockout mutation in one of the key genes in IAA synthesis still produced detectable amounts of IAA (Hartmann and Zimmer, 1994). This led to the conclusion that ther ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
AraC Protein, Regulation of the L-arabinose Operon in Escherichia

... the synthesis of the other ara proteins in E. coli (Greenblatt & Schleif, 1971). Further study of the ara system E. coli has revealed two transport systems (Kolodrubetz & Schleif, 1981a, b). In the lower affinity transport system, the transporter, the araE gene product (Lee et al., 1981; Stoner & Sc ...
Molecular and General Genetics.
Molecular and General Genetics.

... The bacterial strains and plasmids used in this work are listed in Table 1. Strain Sp13Trp is a Trp auxotroph isolated from strain Sp13, a strain similar to Sp7 (Tarrand et al. 1978; Vieille et al. 1989). The rich culture medium used was Luria-Bertani broth for Escherichia coli and nutrient broth (D ...
Salmonella drinking water distribution systems Lisa Mandy Burke
Salmonella drinking water distribution systems Lisa Mandy Burke

... conditions. No significant changes were observed between the wild-type and the tagged strain. The survival fitness studies indicated the incorporation of the gfp gene did not have any noted detrimental effects on the survival and behaviour of the tagged strains. These tagged strains could therefore ...
Pseudouridine at position 55 in tRNA controls the contents of other
Pseudouridine at position 55 in tRNA controls the contents of other

... hypothetically proposed by others. To confirm this idea, we replaced the truB gene in the genome with mutant genes, which express TruB proteins with very weak or no enzymatic activity. However the growth retardation at 50 C was not rescued by these mutant proteins. Nucleoside analysis revealed that ...
Comparative size evolution of marine clades from the Late Permian
Comparative size evolution of marine clades from the Late Permian

... distributions are close to normal. Maximum Size.—We compared maximum sizes between stages for each clade using both raw data and data subsampled to a uniform number of species. Subsampling was required to assess whether changes in the observed maximum size could be explained simply by reductions in ...
Sample pages 1 PDF
Sample pages 1 PDF

... not have a zone or rows of cilia at the dorsal end of the cell. The group contains a several genera, including the most prevalent genus, Entodinium. Holotrichid protozoa are much more motile than entodiniomorphid ciliates. The entodiniomorphs are more numerous in the rumen compared to holotrichs. Th ...
Sequence Searching and Alignments
Sequence Searching and Alignments

... effective protein:DNA, protein:RNA and protein:protein recognition codes (important for recognition of interactions among the various types of molecules); ...
Summary
Summary

... anti-BARF1 antibody responses in NPC patients, the responses are too low to be used for NPC diagnosis. Chapter 6 evaluated the methylation status of multiple TSGs in order to define whether this may be of added value to NPC early detection based on EBV parameters. This study tested the methylation s ...
Composite polymorphisms in the ryanodine receptor 2 gene
Composite polymorphisms in the ryanodine receptor 2 gene

this PDF file - Microbial Ecology in Health and Disease
this PDF file - Microbial Ecology in Health and Disease

... conclusions were based on standard culturing techniques (9, 28, 29). Harmsen et al. compared newer techniques (FISH, 16S rRNA probes) with culturing techniques and found high counts were found by culturing only after 8 –9 days, but FISH detected anaerobes by the second day (30). In addition, another ...
Codon optimization of Col H gene encoding Clostridium
Codon optimization of Col H gene encoding Clostridium

Thesis.  - ResearchSpace@UKZN
Thesis. - ResearchSpace@UKZN

... on the mode ofaction ofcompounds, e.g. whether they act on the cell wall or membrane or are inhibitors ofprotein or nucleic acid synthesis/functions, or interfere with the whole system ofcellular metabolism. However, several mechanisms of action may operate simultaneously, making such classification ...
Detection of Shigella and enteroinvasive Escherichia coli, the
Detection of Shigella and enteroinvasive Escherichia coli, the

... EIEC strains from the environment, water and food, as well as from patients with dysentery and carriers, is of primary importance. For the detection of the Shigella species, routinely used laboratory methods are available. These are based on culturing, biochemical and serological identification of t ...
Abundance matters: a Weld experiment testing the more individuals
Abundance matters: a Weld experiment testing the more individuals

Temporal dynamics in non-additive responses of arthropods to host
Temporal dynamics in non-additive responses of arthropods to host

... abundance to host-plant genotypic diversity varied temporally, we used separate repeated-measures ANOVAs, with richness and abundance as response variables and the number of genotypes as a treatment variable. We also used separate one-way ANOVAs to examine the effect of genotypic diversity treatment ...
the ecology and microbiology of Vibrio cholerae
the ecology and microbiology of Vibrio cholerae

... Jürgens and Matz 2002). For example, protistan grazers  Linking the environment to outbreaks selectively remove active cells, while cladocerans feed indiscriminately on both active and inactive (VBNC) In areas where cholera is endemic, disease outbreaks cells (Cole 1999; Langenheder and Jürgens 200 ...
A systems biology approach sheds new light on the regulation of
A systems biology approach sheds new light on the regulation of

... In my years in Birmingham I had the pleasure to meet many friends and co-workers. I would like to thank them for being there to support me and I am sorry if I cannot mention all of them in this page. However, they will always be in my thoughts. The most special thanks are for my supervisor, Dr Franc ...
Why Sequence Alignment?
Why Sequence Alignment?

... ~2000 blocks from 500 families of related proteins • Blocks: short conserved patterns of 3-60 aa without gaps • Different BLOSUMn matrices are calculated independently from BLOCKS • BLOSUMn is based on sequences that shared at least n ...
Response of diazotrophic microbial community to nitrogen input and
Response of diazotrophic microbial community to nitrogen input and

... Table 2.5. Number of bands detected on DGGE gels ................................................................... 83 Table 2.6. Shannon diversity indices ............................................................................................ 83 Table 3.1. Glyphosate treatment and harvest tim ...
Virulence of Campylobacter species: a molecular genetic approach
Virulence of Campylobacter species: a molecular genetic approach

1: Summary and Options for Congress
1: Summary and Options for Congress

... that this level of concern is based on exaggerated or insufficient data. The abundance and complexity of ecosystems, species, and genetic types have defied complete inventory and thus the direct assessment of changes, As a result, an accurate estimate of the rate of loss is not currently possible. D ...
Functional characterisation of the TUP5 gene in - diss.fu
Functional characterisation of the TUP5 gene in - diss.fu

... arginine content was reduced in tup5-1 compared to the wild type and root growth was restored in the mutant by exogenous supplementation of arginine. As an additional functional proof, a yeast mutant lacking ACOAT which was auxotrophic for arginine could restore its arginine autotrophy after transfo ...
Intra- and Intergeneric Similarities of Pseudomonas and
Intra- and Intergeneric Similarities of Pseudomonas and

... The compositions of the growth media used are listed in Table 2. In some cultures we discovered two different colony types, which we named t, and t2; when these two types displayed different soluble protein electropherograms (K. Kersters, unpublished data), they were grown and hybridized separately. ...
Patterns of disturbance and recovery in littoral rock pools
Patterns of disturbance and recovery in littoral rock pools

... disturbance were Investigated by monitoring the size distributions of patches of open space (mainly consisting of encrusting corahnes and bare rock) In fixed plots over 2 yr. These plots were distributed in pools maintained at natural and reduced densities of herbivores. In both cases, the m e a n p ...
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