0 - Microbiology
... strain of E. coli that had been treated with X-rays, to show that such mutants could restore each other’s function and yield a small but reproducible proportion of prototrophic progeny. Thus, for example, when a mutant that carried the wild-type alleles A , B, C but the mutated forms of three others ...
... strain of E. coli that had been treated with X-rays, to show that such mutants could restore each other’s function and yield a small but reproducible proportion of prototrophic progeny. Thus, for example, when a mutant that carried the wild-type alleles A , B, C but the mutated forms of three others ...
Prokaryotic orthologues of mitochondrial alternative oxidase and plastid terminal oxidase
... by the first and fourth iron-binding motifs, hypothesizing that a high degree of sequence conservation would exist in this central region to maintain protein function but that there might nonetheless exist differences between the distantly related AOX and PTOX groups (Carol and Kuntz, 2001) that cou ...
... by the first and fourth iron-binding motifs, hypothesizing that a high degree of sequence conservation would exist in this central region to maintain protein function but that there might nonetheless exist differences between the distantly related AOX and PTOX groups (Carol and Kuntz, 2001) that cou ...
Frequent gene fissions associated with human pathogenic bacteria
... The identification of the state of each reference protein in all the bacteria targets, was of key importance for the classification of the 141 events, based on their evolutionary history. Essential for this classification was the use of a reliable phylogenetic tree. The phylogenetic relationships of al ...
... The identification of the state of each reference protein in all the bacteria targets, was of key importance for the classification of the 141 events, based on their evolutionary history. Essential for this classification was the use of a reliable phylogenetic tree. The phylogenetic relationships of al ...
scale-dependent responses of plant biodiversity to nitrogen
... Abstract. Experimental studies demonstrating that nitrogen (N) enrichment reduces plant diversity within individual plots have led to the conclusion that anthropogenic N enrichment is a threat to global biodiversity. These conclusions overlook the influence of spatial scale, however, as N enrichment ...
... Abstract. Experimental studies demonstrating that nitrogen (N) enrichment reduces plant diversity within individual plots have led to the conclusion that anthropogenic N enrichment is a threat to global biodiversity. These conclusions overlook the influence of spatial scale, however, as N enrichment ...
Review Non-ribosomal peptide synthetases: Identifying the cryptic
... biosynthetic NRPS-PKS hybrid gene cluster discovered in M. tuberculosis (Quadri et al. 1998). Presence of such a small protein encoding gene was puzzling in NRPS-PKS cluster, and hence its study remained dormant for quite a long time. Later, consistent identification of MbtH homologs in other second ...
... biosynthetic NRPS-PKS hybrid gene cluster discovered in M. tuberculosis (Quadri et al. 1998). Presence of such a small protein encoding gene was puzzling in NRPS-PKS cluster, and hence its study remained dormant for quite a long time. Later, consistent identification of MbtH homologs in other second ...
Novel Ribosome Biogenesis in the Lyme Disease Spirochete
... Here we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as ...
... Here we demonstrate the first characterization of an RNase III enzyme from a spirochete and its role in processing rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as ...
unit 2: mechanisms of inheritance
... Students prepare and present their research outlining the significant scientific contributions/discoveries that led to our understanding of the structure and function of the DNA molecule. Research findings can be presented in a variety of formats: • dramatic presentation (e.g., TV interview, debate ...
... Students prepare and present their research outlining the significant scientific contributions/discoveries that led to our understanding of the structure and function of the DNA molecule. Research findings can be presented in a variety of formats: • dramatic presentation (e.g., TV interview, debate ...
Page 4 of 4 - Thermo Fisher Scientific
... 1 µl of SuperScript™ III RT (200 units/µl)* *If generating cDNA longer than 5 kb at temperatures above 50°C using a gene-specific primer or oligo(dT)20, the amount of SuperScript™ III RT may be raised to 400 U (2 µl) to increase yield. 4. Mix by pipetting gently up and down. If using random primers, ...
... 1 µl of SuperScript™ III RT (200 units/µl)* *If generating cDNA longer than 5 kb at temperatures above 50°C using a gene-specific primer or oligo(dT)20, the amount of SuperScript™ III RT may be raised to 400 U (2 µl) to increase yield. 4. Mix by pipetting gently up and down. If using random primers, ...
Two glucose/xylose transporter genes from the yeast Candida
... obtained by RACE using the FirstChoice® RLM (RNA ligasemediated)-RACE kit (Ambion). Total RNA was extracted from C. intermedia PYCC 4715 cells cultivated in 0.5 % (w/v) xylose and purified using the RNACleanup protocol in the RNeasy kit (Quiagen). This RNA sample was used as a template for the 3 RL ...
... obtained by RACE using the FirstChoice® RLM (RNA ligasemediated)-RACE kit (Ambion). Total RNA was extracted from C. intermedia PYCC 4715 cells cultivated in 0.5 % (w/v) xylose and purified using the RNACleanup protocol in the RNeasy kit (Quiagen). This RNA sample was used as a template for the 3 RL ...
Cambridge Workshop November 2008
... 1. Add relevant publications to FlyBase 2. Associate genes with uncurated publications 3. Flag data types in uncurated publications 4. Submit revisions to gene models 5. Work with FlyBase curators to improve specific areas 6. Annotate / clean up 'gene orphans’ 7. Use FlyBase symbols in your papers 8 ...
... 1. Add relevant publications to FlyBase 2. Associate genes with uncurated publications 3. Flag data types in uncurated publications 4. Submit revisions to gene models 5. Work with FlyBase curators to improve specific areas 6. Annotate / clean up 'gene orphans’ 7. Use FlyBase symbols in your papers 8 ...
The lac repressor binds to operator DNA
... • Nobel Prize in 1965 (with A. Lwoff) for their discoveries concerning genetic control of enzyme and virus synthesis • Compared the effects of many different types of lac mutants on induction and repression of enzyme activity for lactose utilization ...
... • Nobel Prize in 1965 (with A. Lwoff) for their discoveries concerning genetic control of enzyme and virus synthesis • Compared the effects of many different types of lac mutants on induction and repression of enzyme activity for lactose utilization ...
T03 growth2013
... electron donor. For example an aerobic bacterium growing by oxidising sugar to CO2 for ATP generation will also use the same sugar as a starting material for biomass synthesis. The sugar may be partly degraded via glycolysis to pyruvate or even to Acetyl-CoA to use parts of the TCA cycle for biomass ...
... electron donor. For example an aerobic bacterium growing by oxidising sugar to CO2 for ATP generation will also use the same sugar as a starting material for biomass synthesis. The sugar may be partly degraded via glycolysis to pyruvate or even to Acetyl-CoA to use parts of the TCA cycle for biomass ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... and post-translational modification, individual proteins can behave differently. Bulk studies often require synchronization of protein action, which can be difficult to achieve due to the stochasticity and heterogeneity of multistep and multicomponent processes. Single-molecule methods, in contrast, ...
... and post-translational modification, individual proteins can behave differently. Bulk studies often require synchronization of protein action, which can be difficult to achieve due to the stochasticity and heterogeneity of multistep and multicomponent processes. Single-molecule methods, in contrast, ...
Chapter 12
... Figure 12.12: lac repressor monomer has several domains. Structure rendered from Protein Data Bank 1lbg by Hangli Zhan and provided by Kathleen S. Matthews, Rice University. ...
... Figure 12.12: lac repressor monomer has several domains. Structure rendered from Protein Data Bank 1lbg by Hangli Zhan and provided by Kathleen S. Matthews, Rice University. ...
Rhizopus Raw-Starch-Degrading Glucoamylase: Its
... sized by the modified triester method (Q; T or C, N; any base).17) One of these sequences ...
... sized by the modified triester method (Q; T or C, N; any base).17) One of these sequences ...
Remodeled Respiration in ndufs4 with Low
... considers that even small changes in OXPHOS efficiency in animals are associated with disease, aging, and cellular dysfunction (Gellerich et al., 2004). For example, deficiency in complex I function has been associated with several human diseases such as Leber’s heredity optic neuropathy (Wallace et ...
... considers that even small changes in OXPHOS efficiency in animals are associated with disease, aging, and cellular dysfunction (Gellerich et al., 2004). For example, deficiency in complex I function has been associated with several human diseases such as Leber’s heredity optic neuropathy (Wallace et ...
pDsRed-Monomer-C1 Vector Information
... DsRed-Monomer coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus translation in ...
... DsRed-Monomer coding sequence and the SV40 polyadenylation signal (SV40 poly A). Genes cloned into the MCS will be expressed as fusions to the C-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus translation in ...
PROJECTED CURRICULUM MAP - Neshaminy School District
... to the development of new species: isolating mechanisms, genetic drift, founder effect, and migration. 2b) sequence events that can lead to reproductive isolation of two populations ...
... to the development of new species: isolating mechanisms, genetic drift, founder effect, and migration. 2b) sequence events that can lead to reproductive isolation of two populations ...
Experimental study of CO2-saturated water ‒ illite/kaolinite
... volume of the caprock (i.e. at the interface of the reservoir and the caprock, or along the walls of fractures and faults within the caprock, where scCO2 can move by advection). The major part of the caprock is likely filled with CO2saturated or undersaturated porewater [8]. Some of the papers provi ...
... volume of the caprock (i.e. at the interface of the reservoir and the caprock, or along the walls of fractures and faults within the caprock, where scCO2 can move by advection). The major part of the caprock is likely filled with CO2saturated or undersaturated porewater [8]. Some of the papers provi ...
S. altissima
... and forest habitats in the Upper Midwestern United States (1). The flies attack two species of goldenrod, Solidago altissima (“altissima flies”) and S. gigantea (“gigantea flies”) (1). A tumor-like growth (gall) is formed by the host plant after the female fly deposits eggs on the bud (Fig. 1)(1). F ...
... and forest habitats in the Upper Midwestern United States (1). The flies attack two species of goldenrod, Solidago altissima (“altissima flies”) and S. gigantea (“gigantea flies”) (1). A tumor-like growth (gall) is formed by the host plant after the female fly deposits eggs on the bud (Fig. 1)(1). F ...
Leptodora kindtii population dynamics in the island region of
... Michigan ecosystem (Madenjian et al. 2002). Similar declines in L. kindtii and changes in prey composition were observed in Harp Lake, ON (Yan and Pawson 1997) and in Lake Huron (Lehman and Cáceres 1993), where zooplanktivory by B. longimanus outpaces Mysis and fish predation (Bunnell et al. 2011). ...
... Michigan ecosystem (Madenjian et al. 2002). Similar declines in L. kindtii and changes in prey composition were observed in Harp Lake, ON (Yan and Pawson 1997) and in Lake Huron (Lehman and Cáceres 1993), where zooplanktivory by B. longimanus outpaces Mysis and fish predation (Bunnell et al. 2011). ...