A gene fusion consisting of 960 base pairs of 5`
... method 119). The 24-mer oligonucleotide 5'-AGeGAGATCACATCTTTTATCCAA-3' was usea for site-directed mutagenesis and for the screening of the mutant recombinant phages. The 15-mer oligonucleotide 5'-TGCCAGGAGCATCAA-3' was used as a sequencing primer. PIasfnid DNAs were prepared by the cleared lysate me ...
... method 119). The 24-mer oligonucleotide 5'-AGeGAGATCACATCTTTTATCCAA-3' was usea for site-directed mutagenesis and for the screening of the mutant recombinant phages. The 15-mer oligonucleotide 5'-TGCCAGGAGCATCAA-3' was used as a sequencing primer. PIasfnid DNAs were prepared by the cleared lysate me ...
sequence - Université d`Ottawa
... for mammalian nuclear DNA (regions not under functional constraint) ~ 4 x 10 -9 nt sub per site per year ...
... for mammalian nuclear DNA (regions not under functional constraint) ~ 4 x 10 -9 nt sub per site per year ...
The Wired Atlas of the Human Ecosystem | Wired Magazine | Wired
... named Anton van Leeuwenhoek noticed a layer of white scum between his teeth. He mixed some of the gunk with pure rainwater and then placed it under one of his handmade microscopes. “I found, to my great surprise,” he wrote, “that it contained many small animalcules, the motions of which were very pl ...
... named Anton van Leeuwenhoek noticed a layer of white scum between his teeth. He mixed some of the gunk with pure rainwater and then placed it under one of his handmade microscopes. “I found, to my great surprise,” he wrote, “that it contained many small animalcules, the motions of which were very pl ...
Alkaline Southern Blotting Kit with 11 × 14 cm BioBond™ Plus
... Southern Breeze™ blotting kits provide standard reagents for transfer in convenient single use packages. Additionally, the kit components have been optimized to provide consistent efficient transfer in the shortest possible time. Each complete kit includes all of the solutions for the pretreatment a ...
... Southern Breeze™ blotting kits provide standard reagents for transfer in convenient single use packages. Additionally, the kit components have been optimized to provide consistent efficient transfer in the shortest possible time. Each complete kit includes all of the solutions for the pretreatment a ...
This article appeared in a journal published by
... powerful methods in microbiology, both have limitations. Microscope-based taxonomy has often failed to uncover cryptic diversity in the taxa under study, even classifying diverse lineages as single species [6]. While novel culture protocols are still being actively developed, roughly 90–99% of micro ...
... powerful methods in microbiology, both have limitations. Microscope-based taxonomy has often failed to uncover cryptic diversity in the taxa under study, even classifying diverse lineages as single species [6]. While novel culture protocols are still being actively developed, roughly 90–99% of micro ...
Systems Biology
... them in software. Write a step function in Python that implements your strategy. Design an analog circuit that does the whole job by itself. Explain your strategy in English. Describe the relative advantages and disadvantages of these two solution strategies. ...
... them in software. Write a step function in Python that implements your strategy. Design an analog circuit that does the whole job by itself. Explain your strategy in English. Describe the relative advantages and disadvantages of these two solution strategies. ...
File
... The fluid material of the nucleus is surrounded by the familiar double membrane called the nuclear envelope. DNA, chromatin and chromosomes can be found inside the nucleus. Chromatin is DNA that is combined with proteins, including histone and non-histone proteins. Chromatin is coiled into larger, h ...
... The fluid material of the nucleus is surrounded by the familiar double membrane called the nuclear envelope. DNA, chromatin and chromosomes can be found inside the nucleus. Chromatin is DNA that is combined with proteins, including histone and non-histone proteins. Chromatin is coiled into larger, h ...
Testing Gene Expression by Reverse Transcriptase PCR (rt
... The concentration of cells in the culture will be given to you day of lab. 2. Pellet cells by centrifuging at 4,000 rpm. Pour off supernatant (into sink is fine). 3. Add 1 mL of Trizol reagent (this is a solution with phenol, which will lyse the cells and inhibit RNase activity). Incubate on the ben ...
... The concentration of cells in the culture will be given to you day of lab. 2. Pellet cells by centrifuging at 4,000 rpm. Pour off supernatant (into sink is fine). 3. Add 1 mL of Trizol reagent (this is a solution with phenol, which will lyse the cells and inhibit RNase activity). Incubate on the ben ...
07_Pathog_and_virulence_2012 - IS MU
... Genotypic methods • e.g. proof of a gene set called icaoperon responsible for the production of intercellular adhesin in Staphylococcus epidermidis ...
... Genotypic methods • e.g. proof of a gene set called icaoperon responsible for the production of intercellular adhesin in Staphylococcus epidermidis ...
Use of Far-Red Emitting DNA Dye DRAQ5 for Cell Cycle
... An Acumen eX3 scans on an area and not well basis, thus scan times are virtually identical for any SBS format microplate. Typically plate cycle times of 10 minutes are achievable but these can be further cut by scanning reduced well areas. Several hundred plates can be scanned per day with minimal r ...
... An Acumen eX3 scans on an area and not well basis, thus scan times are virtually identical for any SBS format microplate. Typically plate cycle times of 10 minutes are achievable but these can be further cut by scanning reduced well areas. Several hundred plates can be scanned per day with minimal r ...
Research news
... DNA interactions of dinuclear RuII arene antitumor complexes in cell-free media The new dinuclear ruthenium drugs - in which two {(η6-p-isopropyltoluene)RuCl[3-(oxo-κO)-2-methyl-4pyridinonato-κO4]} units were linked by flexible chains of different length [(CH2)n (n = 4, 6, 8, 12)] - were found to ex ...
... DNA interactions of dinuclear RuII arene antitumor complexes in cell-free media The new dinuclear ruthenium drugs - in which two {(η6-p-isopropyltoluene)RuCl[3-(oxo-κO)-2-methyl-4pyridinonato-κO4]} units were linked by flexible chains of different length [(CH2)n (n = 4, 6, 8, 12)] - were found to ex ...
Mysterious Monster Lab
... of nucleotides in the DNA determines the sequence of amino acids in the polypeptides, and thus the structure of the proteins. In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequence in the form of a compleme ...
... of nucleotides in the DNA determines the sequence of amino acids in the polypeptides, and thus the structure of the proteins. In a process called transcription, which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNA’s nucleotide sequence in the form of a compleme ...
introduction
... mislead in distinguishing between pairs of closely allied species (Wu, 1962), geographical races or ecotypes because not all genetic differentiation results in morphological variation. The traditional method of identifying species and their genetic relationships is now gradually being replaced by pr ...
... mislead in distinguishing between pairs of closely allied species (Wu, 1962), geographical races or ecotypes because not all genetic differentiation results in morphological variation. The traditional method of identifying species and their genetic relationships is now gradually being replaced by pr ...
Microbiology
... phenol-degrading bacteria and thousands of other bacteria Inoculate phenol-containing culture medium with the soil, and incubate Transfer 1 ml to another flask of the phenol medium, and ...
... phenol-degrading bacteria and thousands of other bacteria Inoculate phenol-containing culture medium with the soil, and incubate Transfer 1 ml to another flask of the phenol medium, and ...
Worksheet - III
... 3. Amniocentesis for sex determination is banned in our country. Is it justified? 4. List any four symptoms shown by a Down’s syndrome afflicted child. Explain the cause of this disorder. 5. What does Oparin-Haldane hypothesis about origin of life suggest? Who provided support to their hypothesis? 6 ...
... 3. Amniocentesis for sex determination is banned in our country. Is it justified? 4. List any four symptoms shown by a Down’s syndrome afflicted child. Explain the cause of this disorder. 5. What does Oparin-Haldane hypothesis about origin of life suggest? Who provided support to their hypothesis? 6 ...
The Scientific Method in Biology
... the pairs of the string is known, then the other one is also known. This property is used during cell division, when the helices unwind themselves and each half is copied. This copying activity can be considered information transfer, but errors in the code may also occur. If we consider a long strin ...
... the pairs of the string is known, then the other one is also known. This property is used during cell division, when the helices unwind themselves and each half is copied. This copying activity can be considered information transfer, but errors in the code may also occur. If we consider a long strin ...
ecotoxicology - Isis Tassinari
... is a study of the effect of toxic chemicals on biological organisms. It’s interdisciplinary field between ecology and toxicology. • The Organization of economic cooperation and development (OECD) has guidelines to the specific tests which measure toxicity levels in organisms. • The most important ac ...
... is a study of the effect of toxic chemicals on biological organisms. It’s interdisciplinary field between ecology and toxicology. • The Organization of economic cooperation and development (OECD) has guidelines to the specific tests which measure toxicity levels in organisms. • The most important ac ...
Gene Section DDX43 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 43) Atlas of Genetics and Cytogenetics
... Local order: between markers GATA11B08 and D6S284. ...
... Local order: between markers GATA11B08 and D6S284. ...
F215: Control, Genome and the Environment
... chromatid is genetically identical as they contain exact copies of the same length of DNA. 2. Prophase I Chromatin condenses and supercoils. Chromosomes come together in homologous pairs (one maternal chromosome pairs with one paternal chromosome), to form a ‘bivalent.’ Each chromosome has the same ...
... chromatid is genetically identical as they contain exact copies of the same length of DNA. 2. Prophase I Chromatin condenses and supercoils. Chromosomes come together in homologous pairs (one maternal chromosome pairs with one paternal chromosome), to form a ‘bivalent.’ Each chromosome has the same ...
DNA
... Gene Regulation • Gene Regulation – ability of an organism to control which genes are transcribed. – Transcription factors controls what and when genes are expressed to make proteins. – 2 Transcription Factors: 1. Guide & stabilize the binding of RNA polymerase 2. Controls rate of transcription ...
... Gene Regulation • Gene Regulation – ability of an organism to control which genes are transcribed. – Transcription factors controls what and when genes are expressed to make proteins. – 2 Transcription Factors: 1. Guide & stabilize the binding of RNA polymerase 2. Controls rate of transcription ...
Replication 1
... Uses ATP to separate the DNA strands At least 4 helicases have been identified in E. coli. How was DnaB identified as the helicase necessary for replication? NOTE: Mutation in such an essential gene would be lethal. Solution? ...
... Uses ATP to separate the DNA strands At least 4 helicases have been identified in E. coli. How was DnaB identified as the helicase necessary for replication? NOTE: Mutation in such an essential gene would be lethal. Solution? ...
transcription and translation
... Making Proteins Protein Synthesis Process of making proteins Two part process 1. Transcription ...
... Making Proteins Protein Synthesis Process of making proteins Two part process 1. Transcription ...
Cloning and Sequencing of DNA from a Plasmid Library
... In order to investigate the physiology and central metabolic pathways of Geobacter metallireducens Strain GS-15, a plasmid library of EcoRI-digested chromosomal DNA fragments was constructed in E. coli. A probe for a nirS homolog from Pseudomonas stutzeri and an oligonucleotide probe based on cytoch ...
... In order to investigate the physiology and central metabolic pathways of Geobacter metallireducens Strain GS-15, a plasmid library of EcoRI-digested chromosomal DNA fragments was constructed in E. coli. A probe for a nirS homolog from Pseudomonas stutzeri and an oligonucleotide probe based on cytoch ...
Introduction to the course
... -Watson/Crick double stranded DNA: a linear sequence of four nucleotides arranged in genes -biological structure and function is encoded in genes, the unit of inheritance -inheritance based on complex mechanisms to: a) copy DNA b) distribute DNA between parent and offspring -all life forms use essen ...
... -Watson/Crick double stranded DNA: a linear sequence of four nucleotides arranged in genes -biological structure and function is encoded in genes, the unit of inheritance -inheritance based on complex mechanisms to: a) copy DNA b) distribute DNA between parent and offspring -all life forms use essen ...
Analysis of DNA polymerase activity in vitro using non
... ABSTRACT. Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used ...
... ABSTRACT. Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used ...