• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Detection of Antioxidative Activity of Plant Extracts at the DNA-modified Screen-printed Electrode
Detection of Antioxidative Activity of Plant Extracts at the DNA-modified Screen-printed Electrode

... maintain a oxidation/reduction cycle of the iron atom. In such case it is rather difficult to differentiate between antiradical and metal-reduction properties of ascorbic acid as well as phytochemicals. Alternatively, an electrode potential can be used for the reduction of the transition metal which ...
Chapter 12
Chapter 12

... 12.17 Connection: DNA technology is changing the pharmaceutical industry and medicine • Hormones, cancer-fighting drugs, and new vaccines are being produced using DNA ...
KEY TERMS FOR Characteristics of Life
KEY TERMS FOR Characteristics of Life

... 4. As new a.a’s add to the chain, the used tRNAs return to the cytoplasm to find another a.a. 5. When the tRNA’s reach the end of the instructions, the “stop” codon tells them to end the protein. 6. Result of translation: string of a.a’s (protein!) ...
Genome-wide identification of mononuclear cell DNA methylation
Genome-wide identification of mononuclear cell DNA methylation

... donates methyl groups for various biological functions, including methylation of DNA. Methylation of DNA is one example of epigenetic regulation, and epigenetic regulation has been closely linked to development of human diseases6,7. New hypotheses have emerged that diet may affect metabolism via epi ...
Nucleic acids and protein synthesis
Nucleic acids and protein synthesis

... This Article is brought to you for free and open access by The Ames Library, the Andrew W. Mellon Center for Curricular and Faculty Development, the Office of the Provost and the Office of the President. It has been accepted for inclusion in Digital Commons @ IWU by the faculty at Illinois Wesleyan ...
Fluctuation-Facilitated Charge Migration along DNA
Fluctuation-Facilitated Charge Migration along DNA

... 1兾10215 sec. Strong interaction between the electronic degrees of freedom and molecular vibrations may reduce this to 1兾10212 sec, a typical lattice or intramolecular vibration frequency. By comparison, biochemical chargetransfer processes, such as those encountered in the metabolic redox (“oxidatio ...
Plasmid Purification, Restriction Digest, and Lithium Acetate
Plasmid Purification, Restriction Digest, and Lithium Acetate

... cohesive ends on the two molecules. The plasmid and the DNA fragment containing the gene are mixed together in vitro under conditions that favor base-pairing of the cohesive ends. The gene fragment is then covalently inserted into the plasmid by the action of DNA ligase to produce a recombinant plas ...
CHAPTER 10 TEST REVIEW - Hudson City School District
CHAPTER 10 TEST REVIEW - Hudson City School District

Chapter 14 Lecture Notes: Nucleic Acids
Chapter 14 Lecture Notes: Nucleic Acids

... 19. Given the primary structure of DNA or mRNA, use the genetic code table to predict the sequence of amino acids in the polypeptide that would be produced in translation. 20. Describe the three types of RNA and understand the role of each in translation. 21. Define the term “gene expression.” 22. D ...
2. Identifying coding sequences (genes)
2. Identifying coding sequences (genes)

all atom and coarse grained dna simulation studies
all atom and coarse grained dna simulation studies

... It is thus more common to perturb one or few particles at each step. The unwritten rule is to aim for a perturbation that yields about 50% acceptance. The simplicity and general applicability of Monte Carlo approach has long been exploited for molecular applications. One advantage is that it can be ...
Malaria – The Biological Terminator
Malaria – The Biological Terminator

... five Plasmodium species are provided and the data appears to be frequently updated. There is an obligatory initial registration (cookies on, if you please!) and login at each use. A step by step tutorial allows the user to rapidly learn the details of gene searching. The obligatory Plasmodiumspecifi ...
Bio 6 – DNA & Gene Expression Lab  Overview
Bio 6 – DNA & Gene Expression Lab Overview

... information, information in the form of a DNA sequence that can be expressed into proteins and passed on to the next generation. In other words, you are about to learn what genetic information actually means, not just for human beings, but for all life on earth. Once it was known that genes are made ...
Genetic Education for Native Americans
Genetic Education for Native Americans

... The 2 strands of DNA are twisted around one another and the base pairs line up. In the entwined DNA strands an A on one strand within the chromosome always matches with T on the other strand. Similarly, the G on one strand within the chromosome always matches with C on the other strand. The matching ...
The Transcription Process
The Transcription Process

... evidence demonstrate that this code is the basis for the production of various molecules, including RNA and protein. Research has also shown that the instructions stored within DNA are "read" in two steps: transcription and translation. In transcription, a portion of the doublestranded DNA template ...
Slide 1
Slide 1

... Dolly the sheep (1997-2003) ...
Appendix: Fusion Gene Plasmid Construction
Appendix: Fusion Gene Plasmid Construction

... Transgenic mice containing the targeted IGRP locus could have been generated through microinjection of the modified BAC described above into the pronuclei of one-cell embryos (9). However, this BAC contains several other genes so we chose to generate transgenic animals containing only the region of ...
Transition Bias
Transition Bias

... G/U pair, although not as strong as A/U or C/G pair, generally does not disrupt RNA secondary structure (and occurs frequently in RNA secondary structure). Xuhua Xia ...
Identification of sixteen single-nucleotide polymorphism markers in
Identification of sixteen single-nucleotide polymorphism markers in

... Fifty-seven primer groups were designed to identify SNP markers. PCR results showed that 37 primer groups were successfully amplified, 16 (28.1%) of them generated clear and specific products consistently, showing biallelic polymorphisms in 40 samples from wild population. The efficiency in P. fucat ...
NZY M-MuLV Reverse Transcriptase
NZY M-MuLV Reverse Transcriptase

... enzyme gives high yields of first-strand cDNA up to 7 kb. NZY M-MuLV Reverse Transcriptase can be used in firststrand cDNA synthesis experiments, RT-PCR, RT-qPCR, DNA labelling and analysis of RNA by primer extension. ...
Why BLAST is great - GENI
Why BLAST is great - GENI

... annotations of them Searching these databases permits you to find any genes related to your Gene of Interest (GOI), and to potentially assign it a function This is a routine, but highly sophisticated, tool used daily by genome scientists ...
lecture_23 - supporting lehigh cse
lecture_23 - supporting lehigh cse

... Error Correction: Errors occur due to many factors, for examples… – Incorrect insertions/deletions – Damage from thermal energy and UV energy from the sun ...
Non-Mendelian Inheritance Question 1 Red flower color is
Non-Mendelian Inheritance Question 1 Red flower color is

... R. All three processes happen in the cell naturally. S. All three processes require primers. T. Both DNA replication and PCR use DNA polymerases. U. All three processes result in a double stranded product. ...
Automating the Promega Wizard® SV 96 Plasmid DNA Purification
Automating the Promega Wizard® SV 96 Plasmid DNA Purification

... applications. However, purification of plasmid DNA from pelleted bacterial culture can be time-consuming when done manually. Automation of this process can deliver significant increases in throughput as well as higher levels of precision and accuracy. We have developed an automated method that enabl ...
PHYCOCYANIN ALPHA AND BETA SUBUNITS OF Anabaena
PHYCOCYANIN ALPHA AND BETA SUBUNITS OF Anabaena

< 1 ... 83 84 85 86 87 88 89 90 91 ... 353 >

Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report