University of Groningen Characterization of the lytic-lysogenic
... analogous region of other temperate LAB phages due to the presence of convergent promoters (Engel et al., 1998). The lactococcal and streptococcal repressor proteins can be separated into two groups on the basis of their size and homologies (Johansen et al., 2003; Madsen and Hammer, 1998). The large ...
... analogous region of other temperate LAB phages due to the presence of convergent promoters (Engel et al., 1998). The lactococcal and streptococcal repressor proteins can be separated into two groups on the basis of their size and homologies (Johansen et al., 2003; Madsen and Hammer, 1998). The large ...
clinchem.org - Clinical Chemistry
... (MLPA) (19 –21 ) are rapid techniques involving preparation of only 2 reactions and are efficient primary methods to diagnose deletion and duplication genotypes. These assays rely on detection of fluorescence, however, and require relatively expensive reagent sets. Multiplex quantitative PCR is a ne ...
... (MLPA) (19 –21 ) are rapid techniques involving preparation of only 2 reactions and are efficient primary methods to diagnose deletion and duplication genotypes. These assays rely on detection of fluorescence, however, and require relatively expensive reagent sets. Multiplex quantitative PCR is a ne ...
BLAST_tutorial
... Results are stored on Gramene’s server for one week, so that they can be accessed later with the Ticket ID or a bookmark to the results page. ...
... Results are stored on Gramene’s server for one week, so that they can be accessed later with the Ticket ID or a bookmark to the results page. ...
Part 1
... polymerase and the promoter region. It is said to be closed because the DNA duplex remains intact and there is no melting of DNA base pairs. 8. Open promoter complex: The complex formed by tight binding of RNA polymerase with the promoter element. It is said to be open because approximately 17 base ...
... polymerase and the promoter region. It is said to be closed because the DNA duplex remains intact and there is no melting of DNA base pairs. 8. Open promoter complex: The complex formed by tight binding of RNA polymerase with the promoter element. It is said to be open because approximately 17 base ...
Justification of Size Estimates for Tomato Genome Sequencing
... genetic map and spaced along chromosome 2. As such, they still have few and short contigs. Nevertheless, based on the physical distances between mapped marker sequences found in their sequenced BACs, they have estimated that the BACs sequenced to date represent approximately 20% of the genetic map f ...
... genetic map and spaced along chromosome 2. As such, they still have few and short contigs. Nevertheless, based on the physical distances between mapped marker sequences found in their sequenced BACs, they have estimated that the BACs sequenced to date represent approximately 20% of the genetic map f ...
SRAP analysis of DNA base sequence changes in
... might interact with other elements in the DNA molecule and form additional new molecules, or they might shift completely and leave empty space at their original positions. The former leads to genetic effects such as base substitutions; the latter cause deletions and insertions of a single base or a ...
... might interact with other elements in the DNA molecule and form additional new molecules, or they might shift completely and leave empty space at their original positions. The former leads to genetic effects such as base substitutions; the latter cause deletions and insertions of a single base or a ...
Gene Section MRE11A (MRE11 meiotic recombination 11 homolog A (S. cerevisiae))
... Disease Ataxia telangiectasia-like disorder is a progressive cerebellar degenerative disease with telangiectasia, immunodeficiency, cancer risk, radiosensitivity, and chromosomal instability. Only a very few ATLD patients are known, in spite of the suggestion that as many as 6% of "A-T" patients may ...
... Disease Ataxia telangiectasia-like disorder is a progressive cerebellar degenerative disease with telangiectasia, immunodeficiency, cancer risk, radiosensitivity, and chromosomal instability. Only a very few ATLD patients are known, in spite of the suggestion that as many as 6% of "A-T" patients may ...
Rapid detection of multidrug-resistant tuberculosis
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
Textbook Reference: Section 17.3
... The Process of Replication During DNA replication, two molecules of DNA are made from one. The replication follows a semiconservative model (as shown in fig. 17.19 of your p. 582 of text). This means that when a molecule of DNA is copied, each new molecule contains one strand of parental DNA and one ...
... The Process of Replication During DNA replication, two molecules of DNA are made from one. The replication follows a semiconservative model (as shown in fig. 17.19 of your p. 582 of text). This means that when a molecule of DNA is copied, each new molecule contains one strand of parental DNA and one ...
Recombinant DNA Lab
... specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the single stranded ends do easily f ...
... specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the single stranded ends do easily f ...
- Wiley Online Library
... two or more populations. Previously, this was often performed using hybridization techniques such as microarrays, but, more recently, massively parallel sequencing has become the method of choice as costs have dramatically fallen. Constant vector sequences are used as priming sites for PCR to amplif ...
... two or more populations. Previously, this was often performed using hybridization techniques such as microarrays, but, more recently, massively parallel sequencing has become the method of choice as costs have dramatically fallen. Constant vector sequences are used as priming sites for PCR to amplif ...
Chelatococcus sambhunathii sp. nov., a moderately thermophilic
... it could be distinguished from the type strain of its closest phylogenetic neighbour (see Supplementary Table S1 in IJSEM online), Chelatococcus daeguensis CCUG 54519T, based on the absence of C16 : 1v7c, iso-C15 : 0 2-OH and C17 : 0 cyclo. Extraction of genomic DNA and PCR amplification of the 16S ...
... it could be distinguished from the type strain of its closest phylogenetic neighbour (see Supplementary Table S1 in IJSEM online), Chelatococcus daeguensis CCUG 54519T, based on the absence of C16 : 1v7c, iso-C15 : 0 2-OH and C17 : 0 cyclo. Extraction of genomic DNA and PCR amplification of the 16S ...
Cytogenetic and molecular cytogenetic analysis in clinical genetics
... imaging software, can distinguish all 23 chromosomes by chromosome specific colors. This type of analysis can be used to detect abnormalities that affect multiple chromosomes as is sometimes found in cancer cells or immortalized cell lines. ...
... imaging software, can distinguish all 23 chromosomes by chromosome specific colors. This type of analysis can be used to detect abnormalities that affect multiple chromosomes as is sometimes found in cancer cells or immortalized cell lines. ...
reproduction
... and linker histones (Li 2002). The complex pattern of silenced/expressed gene regions that exists within the genome of a single cell type is tightly regulated by epigenetic modifications, either to the histones or to the DNA itself (Felsenfeld & Groudine 2003, Jaenisch & Bird 2003). Although the exa ...
... and linker histones (Li 2002). The complex pattern of silenced/expressed gene regions that exists within the genome of a single cell type is tightly regulated by epigenetic modifications, either to the histones or to the DNA itself (Felsenfeld & Groudine 2003, Jaenisch & Bird 2003). Although the exa ...
Identification of Copy Number Variants using genome graphs.
... While inversions do not cause any changes in copy number, an area that is deleted (SV) will correspond to a loss (CNV). Similarly, a region containing a tandem duplication will be annotated as both having an insertion (SV) and as exhibiting a gain (CNV). In this way, any PEM method for SV detection ...
... While inversions do not cause any changes in copy number, an area that is deleted (SV) will correspond to a loss (CNV). Similarly, a region containing a tandem duplication will be annotated as both having an insertion (SV) and as exhibiting a gain (CNV). In this way, any PEM method for SV detection ...
genetics laboratory manual
... It has a relatively short generation time which is around 2 weeks at 25°C; It is small in size allowing easy handling and requiring small space; Of appropriate size for observing phenotype under dissecting microscope ; Of easy breeding in the lab environment; Large number of offspring is o ...
... It has a relatively short generation time which is around 2 weeks at 25°C; It is small in size allowing easy handling and requiring small space; Of appropriate size for observing phenotype under dissecting microscope ; Of easy breeding in the lab environment; Large number of offspring is o ...
DNA Denaturing through UV-C Photon Dissipation: A
... dichroism of RNA and DNA around 260 nm which corresponds to the peak in the UV-C solar spectrum reaching Earth's surface during the Archean (Michaelian and Simeonov, 2014d), and a small prevalence of right over left handed circularly polarized submarine light in the late afternoon (Angel et al., 197 ...
... dichroism of RNA and DNA around 260 nm which corresponds to the peak in the UV-C solar spectrum reaching Earth's surface during the Archean (Michaelian and Simeonov, 2014d), and a small prevalence of right over left handed circularly polarized submarine light in the late afternoon (Angel et al., 197 ...
Array CGH for detection of chromosome imbalance
... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
... Array CGH analysis of DNA from XXX has been carried out using oligonucleotide arrays with ~44,000 probes across the genome. This test identified an apparently terminal deletion of approximately 84kb from band p16.3 in the short arm of chromosome 4, between base pair coordinates 72,446 and 156,159. T ...
Southern Blot Analysis of Plasmids pRIT4501 and - RIT
... support, and challenging it with a small, labeled, single-stranded DNA (probe) under conditions that promote hybridization. If the probe sequence is found within the target DNA, the probe will bind to its complementary sequence on the support. All that is left is to detect probe binding. Probe is us ...
... support, and challenging it with a small, labeled, single-stranded DNA (probe) under conditions that promote hybridization. If the probe sequence is found within the target DNA, the probe will bind to its complementary sequence on the support. All that is left is to detect probe binding. Probe is us ...
Impact of New Diagnostic Technologies in the Clinical Microbiology
... Whole genome sequencing could not identify • 10 organisms due to their absence from the reference database • The lack of a comprehensive database of human pathogens was particularly problematic for medically important fungi ...
... Whole genome sequencing could not identify • 10 organisms due to their absence from the reference database • The lack of a comprehensive database of human pathogens was particularly problematic for medically important fungi ...
Student`s guide -
... sequences of the alleles D and d. In d, there is a base-pair sequence that can be ‘recognised’ and cut by the restriction enzyme BamHI. In contrast, D has no restriction site and therefore it cannot be cut by the enzyme. Individuals who are homozygous for the dominant allele (DD) only have DNA of ty ...
... sequences of the alleles D and d. In d, there is a base-pair sequence that can be ‘recognised’ and cut by the restriction enzyme BamHI. In contrast, D has no restriction site and therefore it cannot be cut by the enzyme. Individuals who are homozygous for the dominant allele (DD) only have DNA of ty ...
Bisulfite sequencing
Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).