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print version
print version

... • orientation (read from 5’ to 3’ end) • length measured in bp (base pairs) ...
Cloning of genes from genomic DNA Part 1 and 2: DNA Isolation
Cloning of genes from genomic DNA Part 1 and 2: DNA Isolation

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No Slide Title

File
File

... chain of alternating sugar and phosphate molecules (called the backbone). The rungs connected to the sugar molecules are known as bases. ...
Two Epigenetic Mechanisms
Two Epigenetic Mechanisms

... Enables a cell/organism to respond to its dynamic external environment during development and throughout life! Epigenetic changes to the genome can be inherited if these changes occur in cells giving rise to gametes ...
File
File

... end of the RNA primer, Polymerase III covalently bonds the extra nucleotides creating the leading strands. ...
IntroBio520 - Nematode bioinformatics. Analysis tools and data
IntroBio520 - Nematode bioinformatics. Analysis tools and data

... Bioinformatics applies principles of information science (derived from applied math, computer science, and statistics) to make the vast, diverse, and complex life sciences data more understandable and useful. It automates simple but repetitive types of analysis. ...
DNA History: A Timeline Activity
DNA History: A Timeline Activity

... 2. Once you feel comfortable with their contribution and their experiments, fill in the month and year of the scientist(s) main contribution below their picture and cut out the squares. Glue each cut-out in chronological order across the top of a piece of paper. 3. Cut out the pictures below of the ...
N6-(6-Aminohexyl)dATP - Thermo Fisher Scientific
N6-(6-Aminohexyl)dATP - Thermo Fisher Scientific

... that contains a primary amino group attached via a 6-carbon linker at the N6position of the purine base1. AHdATP can be incorporated into DNA by nick translation in the presence of dTTP, dGTP, and dCTP. Life Technologies recommends incorporating AHdATP into DNA probes using the Nick Translation Syst ...
Obesity caused BBC tumors to form at a faster rate compared to lean
Obesity caused BBC tumors to form at a faster rate compared to lean

... extracting DNA or RNA and hybridizing different pieces representing different genes to a transcript • It allows us to measure how many copies of each gene is expressed • This can tell us whether there is no, some or a lot of RNA transcript present ...
DNA and Protein Synthesis Review Worksheet 1. Describe the
DNA and Protein Synthesis Review Worksheet 1. Describe the

... DNA and Protein Synthesis Review Worksheet 1. Describe the structure of DNA and name the scientists who discovered its shape. DNA is a double helix with a sugar, phosphate backbone, and four different nitrogen bases. Watson and Crick were the scientists who are created with the discovery of DNA’s st ...
PDF
PDF

... the width of a human hair, but if you unwound the chromosomes, the DNA would be six feet long. All living things contain DNA recipes and use them to make proteins. This amazing commonality across all forms of life has made possible many practical uses of our DNA knowledge, some of which have been wi ...
Niemann Pick LAB
Niemann Pick LAB

... Part 1 – Polymerase Chain Reaction (PCR) • PCR is a technique used to amplify specific regions of DNA • Start with one molecule of double stranded patient DNA and generate 2 after one cycle • Exponential increase in DNA ...
DNA Technology - 2 What are plasmids?
DNA Technology - 2 What are plasmids?

... ___2nd cycle PCR can generate billions of copies From a SINGLE DNA molecule ___3rd cycle ___4th cycle Enough to do extensive analyses ...
Lesson Plan
Lesson Plan

... Test October 29/30th, 2014 ...
DNA Review
DNA Review

... B. In test tube, DNA replication requires the following. 1. DNA template; 2. Primer 3. dNTPs (dATP, dCTP, dGTP, dTTP) 4. DNA polymerase C. Heat resistant DNApolymerase, such as Taq DNA polymerase is used in Polymerase Chain Reaction (PCR) that is the base of forensic DNA analysis D. In vivo DNA synt ...
Unit 5 Review
Unit 5 Review

... 15. Where is RNA found in a cell? 16. What is the sugar found in RNA? 17. Draw a picture of the monomer of RNA, called a In your picture label the following parts: ribose sugar, base, and phosphate group. group ...
DNA Sequences Analysis
DNA Sequences Analysis

... • FASTA format is the standard format in the field of bioinformatics to represent either nucleotide sequences or peptide sequences. • This format is single-letter code and it allows sequence names and comments • FASAT consists of a single-line description at the beginning followed by sequence data i ...
The Genome of Theobroma Cacao
The Genome of Theobroma Cacao

... The genome, consisting of long strings of chemicals called DNA sequence, includes all the genes of a given organism, but also DNA that is not part of a gene, or noncoding DNA sequence. Each gene contains instructions for assembly of proteins, which consist of strands of amino acids that fold into an ...
Rosa blanda
Rosa blanda

... species will fight against each other to get to the top, they often depend on other species to maintain a balanced ecosystem. DNA barcoding is a new revolutionary technique of identifying species for both experts and non-experts which was introduced by Paul Herbert from University of Guelph in Ontar ...
Cloning and PCR File
Cloning and PCR File

... 2. Annealing involves cooling the single strands of DNA and mixing them with short DNA segments called primers. Primers have base sequences that are complementary to segments of the single DNA strands. As a result, bonds form between the DNA strands and primers. 3. Extension occurs when an enzyme (T ...
Cloning and functional analysis of
Cloning and functional analysis of

... dissolved with the TE buffer. The digested pOJ446 and the partially digested chromosomal DNA were ligated using a DNA ligation kit ver. 2 (Takara Bio Inc.) at 4°C overnight. After ethanol precipitation, the ligated DNA was dissolved with the TE buffer. The resulting ligation mixture was packaged in ...
THE GENOMIC SEQUENCING TECHNIQUE George M. Church and
THE GENOMIC SEQUENCING TECHNIQUE George M. Church and

DKN_5-8 TYPE
DKN_5-8 TYPE

... •  Where are we in the course? Exploring mechanisms of persistance •  What is the central dogma and why does it matter? à molecular rules governing flow of information in cells à duplication of genetic code, transcription, translation ...
Lab 11: DNA Testing
Lab 11: DNA Testing

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Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
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