• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Chapter 18 Practice Multiple Choice
Chapter 18 Practice Multiple Choice

... ____ 13. The researcher measures the concentration of the polypeptides from different regions in the early ...
Protein Synthesis
Protein Synthesis

... 17. UAG is a stop codon. What might happen if the uracil in this codon was changed to cytosine? Glutamine would have been added to the polypeptide chain. 18. List the four different sets of DNA nucleotide sequences that code for the amino acid Valine. Explain why this redundancy in the genetic code ...
WELCOME TO BIOLOGY 2002 - University of Indianapolis
WELCOME TO BIOLOGY 2002 - University of Indianapolis

Stem cells - Plain Local Schools
Stem cells - Plain Local Schools

... II. Regulation of Genes in Eukaryotes A. More elaborate and complicated than in prokaryotes B. Eukaryotic DNA includes promoter sequences before the point that transcription takes place C. Transcription factors- regulate transcription by binding to promoters or RNA polymerases D. Transcription fact ...
DNA damage/repair
DNA damage/repair

Restriction Enzymes
Restriction Enzymes

... • Steps for hybridization 1. The labeled probe is added to the matrix incubated for several hours to allow the probe molecules to find their targets 2. Any unbound probes are then removed. 3. The place where the probe is connected corresponds to the location of the ...
Exam 2
Exam 2

... Chain termination DNA sequencing is used to solve full genome DNA sequences. The DNA sequencing method succeeds because there is no (3'-H, 2'-H, 3'-OH, or 2'-OH) on the last nucleotide added to a growing DNA chain. DNA sequencing is possible because gel electrophoresis can be used to discriminate be ...
Biotech Mini-Lab Students will model the process of using restriction
Biotech Mini-Lab Students will model the process of using restriction

... enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-stranded ends, called "sticky ends." Sticky ends are not really sticky; however, the bases on the stick ...
Biology 207 Workshop 9
Biology 207 Workshop 9

... that all the bands have the same number of DNA molecules can you explain this result? Ethidum bromide intercalates between the paired nucleotides in a double stranded DNA molecule. The longer the DNA molecule the more ethidum bromide is bound and the brighter the band will appear. With the autoradio ...
DNA Replication
DNA Replication

...  Gametes make new cells by meiosis  The first step is still____________________!!!  They now have 92 chromosomes  They divide once (just like mitosis) and have 46 ...
The Complete Forensic DNA Database Solution
The Complete Forensic DNA Database Solution

... Retain samples for decades Forensic DNA technology changes continually. These changes paired with the need to perform new testing with cold cases makes, long-term reference sample storage essential for future testing. Blood and buccal samples stored at room temperature on Whatman FTA cards have been ...
Workflow for processing high throughput Single Molecule Real
Workflow for processing high throughput Single Molecule Real

... There are many sequencing-based approaches to understanding complex metagenomic communities spanning targeted amplification to whole-sample shotgun sequencing. While targeted approaches provide valuable data at low sequencing depth, they are limited by primer design and PCR. Whole-sample shotgun exp ...
1 Biotechnology: Old and New
1 Biotechnology: Old and New

... of yeast’s role in baking by Pasteur and the production of baker’s yeast. g) The Chinese were also using fermentation by 4000 BC to produce things such as yogurt, cheese, fermented rice, and soy sauces. h) Milk has been a dietary staple since at least 9000 BC, producing things such as cheese, cream, ...
Applied Biosystems: Celebrating 25 Years of Advancing Science
Applied Biosystems: Celebrating 25 Years of Advancing Science

DNA - Belle Vernon Area School District
DNA - Belle Vernon Area School District

... genetic information to the cells activity. on genetic information from one generation to the next. ...
An in vitro RNA synthesis reaction was set up and allowed to
An in vitro RNA synthesis reaction was set up and allowed to

... 4. An in vitro RNA synthesis reaction was set up and allowed to proceed in the presence of nonradioactive ribonucleotides (NTPs). After several minutes had passed, radioactive NTPs were added and RNA synthesis was allowed to continue. Then the RNA molecules were isolated from the reaction mixture an ...
MYP unit planner
MYP unit planner

...  Completed during class as teacher walks around to assess student work individually. Based on progress, a second review worksheet might be needed. ...
Mosaic Analysis
Mosaic Analysis

... Limited by number of probes that can be used simultaneously ...
SBI4U- Molecular Genetics
SBI4U- Molecular Genetics

... How might a technique from Hershey and Chase's experiment be used to discover whether the nucleic acid in the bacteriophage is DNA or RNA? (2 marks) Use radioactive thymine and uracil to distinguish between DNA or RNA. Label one test tube where you use only radioactive thymine; the other test tube i ...
Biotechnology
Biotechnology

... genes & organisms, then you need a set of tools to work with this unit is a survey of those tools… ...
BIOL 105 S 2013 Practice Quiz Supp DNA
BIOL 105 S 2013 Practice Quiz Supp DNA

... A) undergoes mutations that can provide variation B) provides energy for the cell C) stores information D) replicates to pass a copy to the next generation Answer B Which of the following statements is incorrect concerning deoxyribonucleic acid. A) It is found within the nucleus of eukaryotes. B) It ...
VII. Molecular Biology Techniques
VII. Molecular Biology Techniques

... amounts, below threshold for probe detection. Sensitivity can be increased by amplification: target, probe and signal ...
Types of Mutations
Types of Mutations

RNA Detection and quantitation
RNA Detection and quantitation

... Northern/Southern blotting • Southern blotting is the name given to a technique, originally described by Southern et al, for the transfer of DNA from a gel to a filter. • Blotting usually refers to the transfer of any molecules from an electrophoresis gel to a solid ...
LS DNA, Heredity and Genetics Booklet PP
LS DNA, Heredity and Genetics Booklet PP

...  PAGE 1- WHAT KIND OF CHARACTERISTICS CAN BE INHERITED FROM PARENTS?  PAGE 2- WHAT ARE CHROMOSOMES, GENES, AND DNA?  PAGE 3- WHAT DOES DNA DO?  PAGE 4- WHAT IS THE SHAPE OF DNA?  PAGE 5- WHAT IS DNA MADE OF?  PAGE 6- MATCH DNA CODE ...
< 1 ... 180 181 182 183 184 185 186 187 188 ... 353 >

Bisulfite sequencing



Bisulphite sequencing (also known as bisulfite sequencing) is the use of bisulphite treatment of DNA to determine its pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of cytosine residues of the dinucleotide CpG, and is implicated in repression of transcriptional activity.Treatment of DNA with bisulphite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected. Thus, bisulphite treatment introduces specific changes in the DNA sequence that depend on the methylation status of individual cytosine residues, yielding single- nucleotide resolution information about the methylation status of a segment of DNA. Various analyses can be performed on the altered sequence to retrieve this information. The objective of this analysis is therefore reduced to differentiating between single nucleotide polymorphisms (cytosines and thymidine) resulting from bisulphite conversion (Figure 1).
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report