![PCR - Polymerase Chain Reaction](http://s1.studyres.com/store/data/008310173_1-d8ef9b76b88e1ce74938c3d746fd3721-300x300.png)
PCR - Polymerase Chain Reaction
... • The oligonucleotides serve as primers for DNA polymerase and the denatured strands of the large DNA fragment serves as the template. – This results in the synthesis of new DNA strands which are complementary to the parent template strands. – These new strands have defined 5' ends (the 5' ends of t ...
... • The oligonucleotides serve as primers for DNA polymerase and the denatured strands of the large DNA fragment serves as the template. – This results in the synthesis of new DNA strands which are complementary to the parent template strands. – These new strands have defined 5' ends (the 5' ends of t ...
Microarray Services
... • Two different tissues are usually too different to be compared directly • If several tissue samples (meant to represent the same tissue) contain varying amounts of different cell types this can also be a problem ...
... • Two different tissues are usually too different to be compared directly • If several tissue samples (meant to represent the same tissue) contain varying amounts of different cell types this can also be a problem ...
RNA secondary structure prediction and gene finding
... – Rare variants are not sufficiently frequent to be captured by current GWA genotyping arrays – And they don’t carry sufficiently large effect sizes to be detected by classical linkage analysis in family studies – The primary technology for the detection of rare SNPs is sequencing, which may target ...
... – Rare variants are not sufficiently frequent to be captured by current GWA genotyping arrays – And they don’t carry sufficiently large effect sizes to be detected by classical linkage analysis in family studies – The primary technology for the detection of rare SNPs is sequencing, which may target ...
ppt
... sequences that are greater than >6% are from different species • Using models based on a poisson distribution and 3 different coverage models, estimates of species for the whole study range from 1800 to 47,000. • A minimum of 12X greater sequence effort would be needed to sample 95% of the unique se ...
... sequences that are greater than >6% are from different species • Using models based on a poisson distribution and 3 different coverage models, estimates of species for the whole study range from 1800 to 47,000. • A minimum of 12X greater sequence effort would be needed to sample 95% of the unique se ...
Sample collection
... 54602 SNPs analyzed Genotyping rate was 0.99 539 SNPs had >10% missing genotyping 1014 SNPs were not in HWE 14651 SNPs with MAF<0.05 Left 38398 SNPs to be analyzed ...
... 54602 SNPs analyzed Genotyping rate was 0.99 539 SNPs had >10% missing genotyping 1014 SNPs were not in HWE 14651 SNPs with MAF<0.05 Left 38398 SNPs to be analyzed ...
Real Time of PCR - KSU Faculty Member websites
... molecular biology. The SYBR® Green probe was the first to be used in realtime PCR. It binds to double-stranded DNA and emits light when excited. Unfortunately, it binds to any double-stranded DNA which could result in inaccurate data, especially compared with the specificity found in the other two m ...
... molecular biology. The SYBR® Green probe was the first to be used in realtime PCR. It binds to double-stranded DNA and emits light when excited. Unfortunately, it binds to any double-stranded DNA which could result in inaccurate data, especially compared with the specificity found in the other two m ...
Cosmid walking and chromosome jumping in the region of PKD1
... overlapping clones in any given cosmid library. It is our experience that when one genomic cosmid library fails to contain an overlap, the use of additional cosmid and bacteriophage libraries increases the chance of finding an overlap by less than 50%. Two well-characterized libraries were used in t ...
... overlapping clones in any given cosmid library. It is our experience that when one genomic cosmid library fails to contain an overlap, the use of additional cosmid and bacteriophage libraries increases the chance of finding an overlap by less than 50%. Two well-characterized libraries were used in t ...
Restriction Fragment Length Polymorphisms (RFLPs)
... 2. RFLPs can be directly associated with the sequence changes that cause a normal gene to be a mutant allele (e.g. sickle-cell anemia). 3. In most cases an RFLP is used only as a nearby genetic marker to find linkage with a phenotype such as an inherited disease. 4. RFLP are used in "DNA fingerprint ...
... 2. RFLPs can be directly associated with the sequence changes that cause a normal gene to be a mutant allele (e.g. sickle-cell anemia). 3. In most cases an RFLP is used only as a nearby genetic marker to find linkage with a phenotype such as an inherited disease. 4. RFLP are used in "DNA fingerprint ...
ZytoLight ® SPEC FGFR3 Dual Color Break Apart Probe
... Several in vivo and in vitro studies have demonstrated the therapeutic potential of FGFR inhibitors in cell lines and animal models harboring FGFR3 fusion genes. Hence, the detection of FGFR3 translocations by Fluorescence in situ Hybridization may be a useful predictive biomarker in the selection o ...
... Several in vivo and in vitro studies have demonstrated the therapeutic potential of FGFR inhibitors in cell lines and animal models harboring FGFR3 fusion genes. Hence, the detection of FGFR3 translocations by Fluorescence in situ Hybridization may be a useful predictive biomarker in the selection o ...
nCounter® Data Analysis Guidelines for Copy Number
... CAUTION: Copy number data generated by the Custom CNV Assay can be negatively affected when DNA input amounts are too low, at which point sampling error can introduce unacceptably high levels of variation in the data. NanoString recommends a minimum of 100 counts for the average of the 10 Invariant ...
... CAUTION: Copy number data generated by the Custom CNV Assay can be negatively affected when DNA input amounts are too low, at which point sampling error can introduce unacceptably high levels of variation in the data. NanoString recommends a minimum of 100 counts for the average of the 10 Invariant ...
Molecular and Genomics-Based Diagnostics for Medical Microbiology
... • FISH is frequently used to detect bacterial species in environmental and clinical samples • Species-specific probes that are complementary to unique target sites on the ribosomal RNA allows direct microscopic visualisation without prior amplification steps, even from blood culture smears for the r ...
... • FISH is frequently used to detect bacterial species in environmental and clinical samples • Species-specific probes that are complementary to unique target sites on the ribosomal RNA allows direct microscopic visualisation without prior amplification steps, even from blood culture smears for the r ...
handout 1
... MOLECULAR SEQUENCE-BASED IDENTIFICATION INTRODUCTION The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. ...
... MOLECULAR SEQUENCE-BASED IDENTIFICATION INTRODUCTION The traditional approach to identifying bacterial strains is based largely on growthdependent physiological and biochemical tests that have been developed since the beginning of the 20th Century, and are still widely used in clinical laboratories. ...
16.7 Screening for clinically important genes
... • The order of nucleotides on the mutated gene is determined by DNA sequencing. Genetic libraries now store the DNA sequences of many of the genes responsible for common genetic diseases. • Fragment of DNA with complementary bases to the mutated portion of the gene is produced. ...
... • The order of nucleotides on the mutated gene is determined by DNA sequencing. Genetic libraries now store the DNA sequences of many of the genes responsible for common genetic diseases. • Fragment of DNA with complementary bases to the mutated portion of the gene is produced. ...
P0196 Poster Session I Basic science: pathogenesis of
... at 3h and 5h of growth from a wild-type strain, as well as from a GdpS mutant. Each sample has been depleted from structural RNAs by using MicrobEnrich method (Ambion). The samples were then subjected to the different methods. RNA-seq data were mapped onto the reference genome sequence using BWA and ...
... at 3h and 5h of growth from a wild-type strain, as well as from a GdpS mutant. Each sample has been depleted from structural RNAs by using MicrobEnrich method (Ambion). The samples were then subjected to the different methods. RNA-seq data were mapped onto the reference genome sequence using BWA and ...
2008 BSHG newesletter 01
... performed in a single run. With conventional Sanger sequencing by capillary electrophoresis up to 384 sequences (more usually 96) can be generated in a single run. In contrast, the new technologies are capable of performing hundreds of thousands to many 10s of millions of analyses in parallel, hence ...
... performed in a single run. With conventional Sanger sequencing by capillary electrophoresis up to 384 sequences (more usually 96) can be generated in a single run. In contrast, the new technologies are capable of performing hundreds of thousands to many 10s of millions of analyses in parallel, hence ...
Chromosomal Microarray (CGH+SNP)
... chromosome analysis, as well as large imbalances detectable by routine chromosome analysis. A special slide (chip) is used that contains thousands of spots neatly arranged in a checkerboard pattern known as “microarray.” Each spot contains DNA segments (DNA probes) that are specific to certain genom ...
... chromosome analysis, as well as large imbalances detectable by routine chromosome analysis. A special slide (chip) is used that contains thousands of spots neatly arranged in a checkerboard pattern known as “microarray.” Each spot contains DNA segments (DNA probes) that are specific to certain genom ...
PPT - Biochemistry and Molecular Biology
... Lots of close sequences will hybridise to a given probe. Wu and Irizarry model the variation in hybridisation of these similar processes using a statistical model. GCRMA determines the contribution to the PM from Signal and from Non-Specific ...
... Lots of close sequences will hybridise to a given probe. Wu and Irizarry model the variation in hybridisation of these similar processes using a statistical model. GCRMA determines the contribution to the PM from Signal and from Non-Specific ...
Supplementary Information (doc 38K)
... plasmid pRL-SV40 was transfected into p53-/- or p53-/-Atf-2-/- cells using Lipofectamine (Invitrogen), and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 4.05 g by adding control plasmid lacking BRCA1. The DNA fragment containing the human Maspin promoter (nucleot ...
... plasmid pRL-SV40 was transfected into p53-/- or p53-/-Atf-2-/- cells using Lipofectamine (Invitrogen), and luciferase assays were performed. The total amount of plasmid DNA was adjusted to 4.05 g by adding control plasmid lacking BRCA1. The DNA fragment containing the human Maspin promoter (nucleot ...
STSE Power point
... Capable of examining from either the entire genome, several areas of the genome, or one specific area of the genome It can examine the genetic information for one individual , or many individuals for one or many SNP’s Useful for making a connection between the presence of an SNP and a disease. It ca ...
... Capable of examining from either the entire genome, several areas of the genome, or one specific area of the genome It can examine the genetic information for one individual , or many individuals for one or many SNP’s Useful for making a connection between the presence of an SNP and a disease. It ca ...
Clinical Next Generation Sequencing (From Bench to Clinitions)
... (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA. ...
... (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA. ...
Powerpoint - University of British Columbia
... isolation (Conversion to cDNA) • Require cloning of cDNAs • Require many different tissues = good coverage of genomic information • Usually sequence from 5’ or 3’ end (known as pair end or mate end sequencing) • Will require more $$ to sequence both ends • Usually less than 60% of genes coverage • W ...
... isolation (Conversion to cDNA) • Require cloning of cDNAs • Require many different tissues = good coverage of genomic information • Usually sequence from 5’ or 3’ end (known as pair end or mate end sequencing) • Will require more $$ to sequence both ends • Usually less than 60% of genes coverage • W ...
PCR-technique Applications
... - DGGE (denaturing gradient gel electrophoresis) - resolving genes of the same size but differing in sequences ...
... - DGGE (denaturing gradient gel electrophoresis) - resolving genes of the same size but differing in sequences ...
MGA 8/e Chapter 12
... the probes is captured by the X-ray film as it decays, producing an exposed region of film. 20. YACs B, D, and E hybridize to one fragment, and YACs A and C hybridize to two fragments. 21. A YAC can hybridize to two fragments if the YAC contains continuous DNA and there is a restriction site within ...
... the probes is captured by the X-ray film as it decays, producing an exposed region of film. 20. YACs B, D, and E hybridize to one fragment, and YACs A and C hybridize to two fragments. 21. A YAC can hybridize to two fragments if the YAC contains continuous DNA and there is a restriction site within ...
HapMap PROJECT - Faculty of Science at Bilkent University
... likely to be neural in phenotypic effect but can inform on nearby alleles that might play a role in disease. ...
... likely to be neural in phenotypic effect but can inform on nearby alleles that might play a role in disease. ...
Molecular Inversion Probe
![](https://en.wikipedia.org/wiki/Special:FilePath/MIP_probe_details_timothy_final.png?width=300)
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.