Track the full extent of structural variation in a genome
... document is subject to change without notice. Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences products and/or third party products. Please refer to th ...
... document is subject to change without notice. Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/or use restrictions may pertain to your use of Pacific Biosciences products and/or third party products. Please refer to th ...
PowerPoint プレゼンテーション
... the web service/interface, the implemented design algorithm and the output in various file formats and an html report. The web service can be sub-divided into the two different aims of de novo design of TALENs against a specific target and the re-evaluation of existing TALENs to find/re-check their ...
... the web service/interface, the implemented design algorithm and the output in various file formats and an html report. The web service can be sub-divided into the two different aims of de novo design of TALENs against a specific target and the re-evaluation of existing TALENs to find/re-check their ...
Pairing and Transvection Position Effects in Drosophila Homologous
... the relationship between pairing and transvection to determine whether there was any correlation between the two. My work involved using DNA fluorescent in situ hybridization, a technique that allows the precise targeting of a location on a chromosome with glowing dye molecules, to visualize the sta ...
... the relationship between pairing and transvection to determine whether there was any correlation between the two. My work involved using DNA fluorescent in situ hybridization, a technique that allows the precise targeting of a location on a chromosome with glowing dye molecules, to visualize the sta ...
ELMER: An R/Bioconductor Tool Inferring Regulatory Element
... DNA methyaltion data feeding to ELMER should be a matrix of DNA methylation beta (β) value for samples (column) and probes (row) processed from row HM450K array data. If TCGA data were used, level 3 processed data from TCGA website will be downloaded and automatically transformed to the matrix by EL ...
... DNA methyaltion data feeding to ELMER should be a matrix of DNA methylation beta (β) value for samples (column) and probes (row) processed from row HM450K array data. If TCGA data were used, level 3 processed data from TCGA website will be downloaded and automatically transformed to the matrix by EL ...
isolation and sequencing of a genomic dna encoding for ascorbat
... ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE ...
... ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE ...
The HapMap Project Tutorial
... Phase III is the most recent phase containing many more individuals from more diverse populations than Phase II and I. However, Phase III does not contain some of the analysis we will need for later on in the ...
... Phase III is the most recent phase containing many more individuals from more diverse populations than Phase II and I. However, Phase III does not contain some of the analysis we will need for later on in the ...
A Sex Chromosome Rearrangement in a Human XX
... short arm (Vergnaud et al., 1986; Affara et al., 1986; Miiller et al., 1986; Page, 1986). Furthermore, in situ hybridization experiments demonstrated that Y-specific DNA was carried by one of the X chromosomes in XX males (Andersson et al., 1986; Buckle et al., 1967). The terminal-interchange model ...
... short arm (Vergnaud et al., 1986; Affara et al., 1986; Miiller et al., 1986; Page, 1986). Furthermore, in situ hybridization experiments demonstrated that Y-specific DNA was carried by one of the X chromosomes in XX males (Andersson et al., 1986; Buckle et al., 1967). The terminal-interchange model ...
An enlarged largest subunit or Plasmodium falciparum RNA
... mRNA (20). Honduras-1 genomic EcoRI and Xbal libraries were constructed in Xgtll and XZAP as previously described (21). The Dral genomic DNA library was constructed in pBluescript. Honduras-1 genomic DNA was digested to completion with Dral, electrophoresed on a 1.0% agarose gel, and DNA of 400 to 1 ...
... mRNA (20). Honduras-1 genomic EcoRI and Xbal libraries were constructed in Xgtll and XZAP as previously described (21). The Dral genomic DNA library was constructed in pBluescript. Honduras-1 genomic DNA was digested to completion with Dral, electrophoresed on a 1.0% agarose gel, and DNA of 400 to 1 ...
Prof. Kamakaka`s Lecture 14 Notes
... Much of our genetic variation is caused by single-nucleotide differences in our DNA : these are called single nucleotide polymorphisms, or SNPs. As a result, each of us has a unique genotype that typically differs in about three million nucleotides from every other person. SNPs occur about once ever ...
... Much of our genetic variation is caused by single-nucleotide differences in our DNA : these are called single nucleotide polymorphisms, or SNPs. As a result, each of us has a unique genotype that typically differs in about three million nucleotides from every other person. SNPs occur about once ever ...
File formats for NGS data - Bioinformatics Training Materials
... ○ GFF/GTF (gene annotation) ○ Wiggle files, BEDgraphs, BigWigs (genomic scores) ...
... ○ GFF/GTF (gene annotation) ○ Wiggle files, BEDgraphs, BigWigs (genomic scores) ...
Supplementary methods
... first tested. Specifically, approximately 25% of the primers were tested on each species. If a primer pair successfully amplified a unique PCR product of the expected size in the leading species, we obtained the product for this gene from the other three species as well. If not, this primer set was ...
... first tested. Specifically, approximately 25% of the primers were tested on each species. If a primer pair successfully amplified a unique PCR product of the expected size in the leading species, we obtained the product for this gene from the other three species as well. If not, this primer set was ...
Homework Assignment #1
... 1. (2 pts) Promoters for protein-coding genes in eukaryotic cells contain a basal promoter element that is recognized by RNA polymerase II and a collection of basal transcription factors (e.g., TFIID, TFIIB). However, the basal activity of the promoter by itself is very low and is invariably influen ...
... 1. (2 pts) Promoters for protein-coding genes in eukaryotic cells contain a basal promoter element that is recognized by RNA polymerase II and a collection of basal transcription factors (e.g., TFIID, TFIIB). However, the basal activity of the promoter by itself is very low and is invariably influen ...
Patents 101 - The Zhao Bioinformatics Laboratory
... E (14737 genes) expressed/EST matches: Expression of the gene is supported by Medicago EST sequence that matches the gene call (partially). H (14209 genes) homology/heterologous: the gene call is supported by similarity to Medicago or other ESTs, protein, FL-cDNA, genomic or other sequences with par ...
... E (14737 genes) expressed/EST matches: Expression of the gene is supported by Medicago EST sequence that matches the gene call (partially). H (14209 genes) homology/heterologous: the gene call is supported by similarity to Medicago or other ESTs, protein, FL-cDNA, genomic or other sequences with par ...
Consalez, GG, Stayton, CL, Freimer, NB, Goonewardena, Brown, WT, Gilliam, TC and Warren, ST: Isolation and characterization of a highly polymorphic human locus (DXS 455) in proximal Xq28. Genomics 12:710-714 (1992).
... One region of the human genome where genetic mapping of disease loci has been particularly fruitful is the terminal band of the human X chromosome long arm. Band Xq28 is one of the more gene-dense regions of the human genome yet recognized, with over 27 loci identified (Davies et at., 1990). Many of ...
... One region of the human genome where genetic mapping of disease loci has been particularly fruitful is the terminal band of the human X chromosome long arm. Band Xq28 is one of the more gene-dense regions of the human genome yet recognized, with over 27 loci identified (Davies et at., 1990). Many of ...
Mapping Enzyme Active Sites in Complex Proteomes
... to map the sites of probe labeling on enzymes isolated from whole proteomes. In comparing this approach to gel-based ABPP, several advantages of the former method are apparent. First and foremost, gel-free ABPP consolidates into a single step the identification of both the protein targets of activit ...
... to map the sites of probe labeling on enzymes isolated from whole proteomes. In comparing this approach to gel-based ABPP, several advantages of the former method are apparent. First and foremost, gel-free ABPP consolidates into a single step the identification of both the protein targets of activit ...
DINE-1 - Biological Sciences
... Artificial Chromosome (BAC) vector. Many of the gaps in our cosmid contig map have now been filled using these BAC clones. Our progress in positioning known genes on the map will be presented. As part of this mapping project we sequenced two cosmid clones, representing ~5% of the euchromatic region. ...
... Artificial Chromosome (BAC) vector. Many of the gaps in our cosmid contig map have now been filled using these BAC clones. Our progress in positioning known genes on the map will be presented. As part of this mapping project we sequenced two cosmid clones, representing ~5% of the euchromatic region. ...
Lecture 6 Gene expression: microarray and deep sequencing
... An “old” technology - some predict microarrays will be replaced by deep sequencing Currently – much cheaper/faster than sequencing; widely used http://www.microarraystation.com/dna-microarray-timeline/ Timeline of DNA Microarray Developments 1991: Photolithographic printing (Affymetrix) 1994: First ...
... An “old” technology - some predict microarrays will be replaced by deep sequencing Currently – much cheaper/faster than sequencing; widely used http://www.microarraystation.com/dna-microarray-timeline/ Timeline of DNA Microarray Developments 1991: Photolithographic printing (Affymetrix) 1994: First ...
PowerPoint Presentation - The GS FLX Sequencer. What is it and
... • small, medium and long transcripts detected equally. • No sequencing bias to either 3’ or 5’ ends of transcripts. • ESTs not contaminated by genomic DNA intron/exon boundaries clearly preserved ...
... • small, medium and long transcripts detected equally. • No sequencing bias to either 3’ or 5’ ends of transcripts. • ESTs not contaminated by genomic DNA intron/exon boundaries clearly preserved ...
PTC Lab Instructions/Information
... 2. Using what you know about genetics, SNPs, and the PTC gene, explain why it is possible for a person to be a “weak taster.” 3. Some studies have shown that PTC “tasters” are less likely to become smokers. Why do you think scientists are seeing this correlation? 4. How can the techniques described ...
... 2. Using what you know about genetics, SNPs, and the PTC gene, explain why it is possible for a person to be a “weak taster.” 3. Some studies have shown that PTC “tasters” are less likely to become smokers. Why do you think scientists are seeing this correlation? 4. How can the techniques described ...
Data/hora: 15/03/2017 01:45:52 Provedor de dados: 69 País: Chile
... from 0.06 to 0.89 and the overall cultivars averaged 0.41. The UPGMA cluster analysis recovered by principal coordinate analysis illustrated that cultivars tend to group according to their class of maturity, region of cultivation, and fruit color. Analysis of molecular variations (AMOVA) revealed th ...
... from 0.06 to 0.89 and the overall cultivars averaged 0.41. The UPGMA cluster analysis recovered by principal coordinate analysis illustrated that cultivars tend to group according to their class of maturity, region of cultivation, and fruit color. Analysis of molecular variations (AMOVA) revealed th ...
Structural Location of Disease-associated Single
... Goddeau, Kasif, Liang JMB, 2003, 327, 1021-1030 Presented by Nancy Baker ...
... Goddeau, Kasif, Liang JMB, 2003, 327, 1021-1030 Presented by Nancy Baker ...
Primer Design
... In the later rounds most of the DNA is target gene only, and includes the previous primer sequences. Remember that primers are incorporated into the amplified genes! ...
... In the later rounds most of the DNA is target gene only, and includes the previous primer sequences. Remember that primers are incorporated into the amplified genes! ...
Assay Quality Considerations
... Introduction of unwanted nucleic acids into specimen - the sensitivity of PCR techniques makes them vulnerable to contamination Repeated amplification of the same target sequence leads to accumulation of amplification products in the laboratory environment A typical PCR generates as many as 109 cop ...
... Introduction of unwanted nucleic acids into specimen - the sensitivity of PCR techniques makes them vulnerable to contamination Repeated amplification of the same target sequence leads to accumulation of amplification products in the laboratory environment A typical PCR generates as many as 109 cop ...
Molecular Inversion Probe
Molecular Inversion Probe (MIP) belongs to the class of Capture by Circularization molecular techniques for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome. Probes used in this technique are single stranded DNA molecules and, similar to other genomic partitioning techniques, contain sequences that are complementary to the target in the genome; these probes hybridize to and capture the genomic target. MIP stands unique from other genomic partitioning strategies in that MIP probes share the common design of two genomic target complementary segments separated by a linker region. With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes. Specifically, the two target complementary regions at the 5’ and 3’ ends of the probe become adjacent to one another while the internal linker region forms a free hanging loop. The technology has been used extensively in the HapMap project for large-scale SNP genotyping as well as for studying gene copy alterationsand characteristics of specific genomic loci to identify biomarkers for different diseases such as cancer. Key strengths of the MIP technology include its high specificity to the target and its scalability for high-throughput, multiplexed analyses where tens of thousands of genomic loci are assayed simultaneously.