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course: bio 201
course: bio 201

... virtually every cell in the body. These mutations are also called germ line mutations because they are present in the parent’s egg or sperm cells, which are also called germ cells. When an egg and a sperm cell unite, the resulting fertilized egg cell receives DNA from both parents. If this DNA has a ...
DNA Mutation
DNA Mutation

... the gap by recombination with either the other homolog or the sister chromatid--this yields two intact daughter molecules, one of which still contains the dimer. 1. Recombinational (daughter-strand gap) repair This is a repair mechanism which promotes recombination to fix the daughter-strand gap--no ...
Genome mapping - Home - KSU Faculty Member websites
Genome mapping - Home - KSU Faculty Member websites

... oligonucleotide hybridization analysis. • An oligonucleotide is a short single-stranded DNA molecule, usually less than 50 nucleotides in length, that is synthesized in the test tube. If the conditions are just right, then an oligonucleotide will hybridize with another DNA molecule only if the olig ...
Discovering DNA: Structure and Replication
Discovering DNA: Structure and Replication

... http://www.mun.ca/biology/scarr/Fg10_03smc.gif ...
Dancing Naked in the Mind Field
Dancing Naked in the Mind Field

... mRNA that codes for the making of a single protein (animation) 4)The mRNA is extracted from the cell and purified 5)Reverse transcriptase is added which synthesises a single stranded DNA molecule complementary to the mRNA 6)The second DNA strand is made by using the first as a template, and adding t ...
Exercise 5. DNA Ligation, Selection and
Exercise 5. DNA Ligation, Selection and

... will produce the corresponding protein. The process by which (1) a gene is identified, (2) a DNA fragment is obtained containing the gene sequence, and (3) the gene is introduced into a new host is called cloning. Subcloning occurs when a gene which has already been cloned is transferred from one ve ...
Assignment - San Diego Mesa College
Assignment - San Diego Mesa College

... of the RFLP method for a person with two normal genes (= homozygous normal; NN), a person with one normal and one mutated gene (= heterozygous; NM) and a human individual with two mutated genes (= homozygous mutated; MM) into the gray box below. (Label the blot by showing the genotypes and the DNA f ...
Coloration in Jaguars Have you ever seen a jaguar in a zoo? Most
Coloration in Jaguars Have you ever seen a jaguar in a zoo? Most

... What effect did the change in DNA that led to the insecticide resistance have? Mosquitoes and other organisms have an enzyme called acetylcholinesterase, or AChE, in the synapses between neurons. This enzyme helps remove a neurotransmitter called acetylcholine from the synapse. Insecticides cause th ...
Genealogy: To DNA or not to DNA?
Genealogy: To DNA or not to DNA?

... 3. Autosomal DNA testing is the kind that works across all lines of a family, both male and female. People that share the same autosomal DNA will have common ancestors. However, further analysis would be required to determine to which family line they are connected. The autosomal DNA test is the lat ...
History of DNA
History of DNA

... • DNA is incorporated by recombination (replacing an existing bit of chromosome) ...
Domain Three (3_genetics)
Domain Three (3_genetics)

... 22. As each section of the genetic code on DNA is transcribed to mRNA, the two strands of DNA rejoin. Then the mRNA moves into the cytoplasm through a pore in the nuclear membrane. Ribosomes attach to the mRNA, in the cytoplasm, to carry out the formation of a protein. What is this process called? A ...
Lab 7 — DNA Extraction and Gel Analysis
Lab 7 — DNA Extraction and Gel Analysis

...  DNA fragments separated by size  Smaller fragments go farther  Application: DNA fingerprinting Differentiating samples from 6 individuals. ...
Chapter 17-part 2
Chapter 17-part 2

... synthesize only short fragments, because the only way they can work is from 5’ to 3’. These resulting short fragments consist of about 200 nucleotides each, named Okazaki fragments after their discoverer. 6. Ligation The Okazaki fragments and any nicks remaining are eventually joined by DNA ligase. ...
File
File

... Too much time in the tanning booth might damage epithelial cells due to the UV radiation. the mosty likely effect would be….. ...
Overview of Current Research
Overview of Current Research

... Toxicogenomics is a form of analysis by which the activity of a particular toxin or chemical substance on living tissue can be identified based upon a profiling of its known effects on genetic material. Once viable, the technique should serve for toxicology and toxin-determination a role analogous t ...
GENETICS 603 EXAM 1 Part 1: Closed book October 3, 2014 NAME
GENETICS 603 EXAM 1 Part 1: Closed book October 3, 2014 NAME

... sequence  his•cys•met•asp•gly.    No  activity  was  found  in  an  acridine  (ICR-­‐170)  induced   mutation,  but  in  a  revertant  found  after  a  second  treatment  with  ICR-­‐170,  the  equivalent   sequence  of  amino  acids  was ...
Genetics and Genomics in Medicine Chapter 9 Questions
Genetics and Genomics in Medicine Chapter 9 Questions

... c) Genome editing has the potential to permit specific “gene correction” in which a mutant sequence in a cell is restored to the normal sequence. d) Genome editing might also have therapeutic potential by specifically inactivating a gene in some cases. Answer 9.19 b) The specificity of genome editi ...
DNA
DNA

... The bases are held together by a weak hydrogen bond. Two (2) bonds between A and T, three (3) bonds between C and G. ...
Genome structure, analysis and evolufion Lecture 1
Genome structure, analysis and evolufion Lecture 1

... Since   2000   the   scien?fic   and   popular   press   has   reported   and   celebrated   the   ‘complete’   sequencing   of   the   first   insect   (Drosophila   melanogaster)   and   plant   genome   (Arabidopsis   thaliana)   and   the   hum ...
HW#2 (first draft)
HW#2 (first draft)

... (ii) Imagine that the double-stranded DNA template for a PCR reaction has two blocks of sequence of 70bp that are identical (a perfect repeat, indicated by the rectangles below), separated by a stretch of normal, unique DNA sequence of about 800bp. You use 25nt long primers complementary to sequence ...
71370_Forensic_DNA_Analysis
71370_Forensic_DNA_Analysis

... (PCR) • DNA Polymerase = enzyme that builds new DNA strand one base pair at a time ...
PTC Lab Instructions/Information
PTC Lab Instructions/Information

... – One band – Full length of gene • 220 bp ...
Exam 2 Full v3 Bio200 Win16
Exam 2 Full v3 Bio200 Win16

... In the diagram, a snapshot of a single chromosome is shown along with RNA polymerase and ribosomes. There are RNAs of various sequences, as well as three different proteins. Use this diagram to answer the questions on pages 2-3. /5 1a) At the moment of this snapshot shown to the right, which of the ...
Name Period ______ Ms Foglia • AP Biology Date LAB: CLONING
Name Period ______ Ms Foglia • AP Biology Date LAB: CLONING

... LAB: CLONING PAPER PLASMID In this exercise you will use paper to simulate the cloning of a gene from one organism into a bacterial plasmid using a restriction enzyme digest. The plasmid (puc18 plasmid) can then be used to transform bacteria so that it now expresses a new gene and produces a new pro ...
Lecture 20  DNA Repair and Genetic Recombination
Lecture 20 DNA Repair and Genetic Recombination

... recombination: – Homologous recombination – also known as generalized; occurs at meiosis (as we have just discussed) or not…. – Site specific (specialized) recombination – typically in bacteria and viruses; enzymes involved act only on a particular pair of target sequences in an intermolecular react ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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