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CSI: SNAB - NKS | VLE - our Online Classroom
CSI: SNAB - NKS | VLE - our Online Classroom

... Using primers specific for intron 1 of the gene, the gene sequence for the intron can be amplified. The X chromosome gene, AMELX, gives rise to a 106 bp amplification product (amplicon) and the Y chromosome gene, AMELY, a 112 bp amplicon. Hence, the AMELX contains a 6 bp deletion in the intron 1. Wh ...
Unit 6 Notes
Unit 6 Notes

File - Gander biology
File - Gander biology

... 1. Transcription- a strand of DNA is used as a template to make a strand of RNA, a single stranded nucleic acid (in the nucleus of the cell) 2. Translation-the order of the nucleotides in RNA is decoded in a sequence of amino ...
Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

... charged particles located in a gel when an electric current is applied • Charged particles can include DNA, amino acids, peptides, etc ...
Chapter 10
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... Viral DNA is duplicated along with the host chromosome during each cell division The inserted phage DNA is called a prophage Most prophage genes are inactive Environmental signals can cause a switch to the lytic cycle ...
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... Knowing the atomic weight of a nucleotide, and markers, gel electrophoresis separates pieces of DNA by weight, with the heavier (longer) segments moving slower and the lighter (shorter) segments moving faster through the gel. These bands are compared with “markers” (pieces of DNA with known molecula ...
Xeroderma Pigmentosum (XP)
Xeroderma Pigmentosum (XP)

... • Cells are capable of only a limited series of divisions before they arrest or senesce and chromosomal fusions and cell death • Telomeres caps of chromosome ends and function to prevent DNA loss during DNA replication and provide a cellular clock for cell ...
Florida Department of Law Enforcement`s Convicted Offender DNA
Florida Department of Law Enforcement`s Convicted Offender DNA

... chemiluminescence and automated techniques that have increased output 300% with no additional personnel. In 1995 more offenses were added to the list requiring inclusion into the database as well as personnel increases statewide which allowed more non-suspect case analysis. This resulted in a steady ...
DNA History - Biology Junction
DNA History - Biology Junction

...  The march to understanding that DNA is the genetic material T.H. Morgan (1908)  Frederick Griffith (1928)  Avery, McCarty & MacLeod (1944)  Hershey & Chase (1952)  Watson & Crick (1953)  Meselson & Stahl (1958) ...
Biochemistry - Problem Drill 22: DNA Question No. 1 of 10
Biochemistry - Problem Drill 22: DNA Question No. 1 of 10

... along the helix axis and related by a rotation of 36 degrees. Hence, the helical structure repeats after 10 residues on each chain, that is, at interval of 34 Å. (D) The two chains are held together by phosphate bonds between pairs of bases. Adenine is always paired with thymine; guanine is always p ...
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Viral DNA replica"on

... m.socraIve.com   room  number:  virus Which  statement  about  viral  DNA  synthesis  is  NOT  correct?   1. Large  DNA  viruses  encode  many  proteins  involved  in  DNA   synthesis   2. Small  DNA  viruses  encode  at  least  one  protein ...
Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)

... Reagents PCR needs several things target DNA to be amplified Taq polymerase, a DNA polymerase that is stable at high temperatures dNTPs, free nucleotides that DNA polymerase uses to make new strands of DNA DNA primers, one for the 5’ end of the target gene and a complement of the 3’ end ...
Chapter 12 Molecular Genetics
Chapter 12 Molecular Genetics

... As the DNA strand unwinds, the RNA polymerase initiates mRNA synthesis and moves along one of the DNA strands in the 3’ to 5’ direction. ► Template strand – read by RNA polymerase, and mRNA is synthesized by a complement to the DNA nucleotides. ► Nontemplate strand – not read by RNA Polymerase ► The ...
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11.2 What Is the Structure of DNA?

... – Since every adenine, for example, is paired with a thymine, no matter how many adenines are in the DNA molecule, there will be an equal number of thymines ...
DNA & RNA Notes
DNA & RNA Notes

... How does Transcription work? * QUESTION…have you been to court? * There is a person typing what is said and is creating a “court transcript”…which is really a code…shortened version…and later the transcript is translated into all the words that were said for a record. ...
G - AP Bio Take 5
G - AP Bio Take 5

... What are Transcription Factors? They are Initiation complex („start‟ complexes) ...
Plasmid Isolation - MITCON Biopharma
Plasmid Isolation - MITCON Biopharma

... has the capability of replicating autonomously and it is this property that leads to its isolation and physical recognition. The closed covalent nature of their structure allows them to be separated from chromosomal DNA by either gel electrophoresis or cesium chloride buoyant density gradients. Plas ...
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DNA and Genetics in Biotechnology

Ch 12: DNA and RNA
Ch 12: DNA and RNA

... B. The Structure of RNA 6. RNA consists of a long chain of nucleotides. 7. Each nucleotide is made up of: a. a 5-carbon sugar called ribose b. a phosphate group c. a nitrogenous base 8. There are three main differences between RNA and DNA: a. The sugar in RNA is ribose instead of deoxyribose. b. RNA ...
Powerpoint
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78 DNA Cheek Cell lab - Renton School District
78 DNA Cheek Cell lab - Renton School District

... physical traits and regulates body functions. Even though we have had over 50 years of research on DNA, much remains to be learned, and every answer seems to create more questions. The length of DNA per cell is about 100,000x as long as the cell itself. However, it takes up only about 10% of the cel ...
Section 1: What Do Genes Look Like? (p. 128)
Section 1: What Do Genes Look Like? (p. 128)

... The gene material must be able to supply instructions for cell processes and for building cell structures. The gene material must also be able to be copied each time a cell divides, so that each cell contains an identical set of genes. ...
Both the packaging of DNA around histone proteins, as
Both the packaging of DNA around histone proteins, as

... histone proteins and DNA have different modifications that signal a closed chromosomal configuration. In this closed configuration, the RNA polymerase and transcription factors do not have access to the DNA and transcription cannot occur. ...
Gene%20Sequencing[2]
Gene%20Sequencing[2]

... Determines the physical order of the sequence features of the entire DNA of an individual Places certain DNA fragments onto chromosomes by identifying the fragments ...
Document
Document

... • electric field of 180 V/cm • ~ 2 min to complete the separation of the DNA fragment • compare to 8 to 10 hours to complete an equivalent separation using conventional gel electrophoresis • compare to 1 to 2 hours with conventional capillary ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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