Cockayne Syndrome group B protein interacts
... we report that CSB physically interacts with TRF2,
a duplex telomeric DNA binding protein essential
for telomere protection. We find that CSB localizes
at a small subset of human telomeres and that it
is required for preventing the formation of
telomere dysfunction-induced foci (TIF) in CS cells.
Assay of ÃŁ-glucuronidase activity in intact
... plasmid may have been deleted from most or all of the cells; alternatively the entire construct
may have been lost from a small percentage of the cells.
The pellets contained appreciable GUS activity (Figure 1), especially if the abrasive was ground
glass (Experiment 1, Figure 1) rather than alumina ...
... (MEFs) and splenocytes from i-TERT mice and non-transgenic controls. TERT mRNA was
induced in a doxycycline-dependent manner in both MEFs and splenocyte cultures (Fig. S1a).
Analysis of metaphase preparations from MEFs and splenocytes showed no end-to-end fusions
with TERT induction, indicating that ...
Induced Pluripotent Stem Cell Lines Derived from
... medicine once technical limitations (for example,
mutation through viral integration) are eliminated.
Mammalian embryogenesis elaborates distinct developmental
stages in a strict temporal order. Nonetheless, because
development is dictated by epigenetic rather than genetic
events, differentiation is ...
Mechanisms of cell aging in cell culture
... telomerase, which is responsible for the maintenance
of telomeres in most immortal cell lines, is also
involved. Telomeres are specialized structures at the
ends of chromosomes, which consist of tandem
repeated DNA sequences - (TTAGGG) and proteins
associated with them such as telomeric repeat bindi ...
... We observed high frequencies of chromosome ends with undetectable TTAGGG
repeats and extra-chromosomal telomeric DNA signals leading to an excess in
the total yield of telomeric signals in FA cells. This result is interpreted as an
excess of breaks in telomeric repeat arrays in FA lymphocytes.
Assembly of Mutant-Template Telomerase RNA into Catalytically
... somatic cells, human chromosomes can lose up to 50 to 200
nucleotides of telomeric sequence per cell division (3). Such
shortening of telomeres has been proposed to be a mitotic clock
that monitors cell divisions; sufficiently short telomeres and lack
of telomerase may signal cellular senescence (3, ...
Supplementary Figure and Table Legend (doc 44K)
... (e) Image shows a representative RT-PCR analysis of expression of stemness-related genes in
primary tissue-derived mammospheres and NBLE-derived mammospheres; (n=3).
Supplementary Figure S3. (a) Immunocytochemical analysis for CK14 expression in NBLE
cells cultured in differentiating conditions comp ...
MCB 135K: Discussion
... double-stranded DNA and has a high affinity
for qudruplex "G-DNA", a structure that may
form at telomeres, ribosomal DNA [HQ]
(rDNA) and other GC-rich sequences
... Telomerase = RNA primer 5'-CCCTAA-3' + RT enzyme (elongates the Grich 3'end) + another protein component.
Telomerase is a ribonucleoprotein that uses its internal RNA component as
a template for the synthesis of DNA on the ends of chromosomes during
In mammals, telomerase is norma ...
... Telomeres are specialized DNA sequences that cap the ends
of linear chromosomes and provide protection against gene
erosion at cell divisions, chromosomal non-homologous
end-joinings and nuclease attacks.
... cancerous cells
• Cells spread via the circulatory or
• 99% of cancer cells die en route, but some
lodge in capillaries or lymph nodes
• Cancer cells can break down material
between cells to travel within tissues,
leading to new colonies
Nature Medicine Research Highlights: New from NPG
... stages of Alzheimer’s disease. The researchers found that the human tau was able to
cross the synapse into brain regions to which
the entorhinal cortex projects, such as the
hippocampus, an area of the brain involved in
learning and memory. They could also detect
human tau in areas of the brain that ...
Researchers Find "Immortality" Enzyme in Cancer Cells Cancer
... The telomere is made up of DNA segments that help control the accuracy of
genetic reproduction when a cell divides to create a new cell.
In cancer cells, it is thought that the telomere does not erode with each cell
division, thus enabling cancer cells, in a sense, to be "immortal." Telomeres in
The Biology of Cancer
... Highly repetitive DNA on the
Protect chromosomes from damage
Get ___________________________________ with
each cell division
Healthy cells can only divide approx
_________________ times (Hayflick Limit) before the
Telomerase reverse transcriptase
Telomerase reverse transcriptase (abbreviated to TERT, or hTERT in humans) is a catalytic subunit of the enzyme telomerase, which, together with the telomerase RNA component (TERC), comprises the most important unit of the telomerase complex.Telomerases are part of a distinct subgroup of RNA-dependent polymerases. Telomerase lengthens telomeres in DNA strands, thereby allowing senescent cells that would otherwise become postmitotic and undergo apoptosis to exceed the Hayflick limit and become potentially immortal, as is often the case with cancerous cells. To be specific, TERT is responsible for catalyzing the addition of nucleotides in a TTAGGG sequence to the ends of a chromosome’s telomeres. This addition of repetitive DNA sequences prevents degradation of the chromosomal ends following multiple rounds of replication.hTERT absence (usually as a result of a chromosomal mutation) is associated with the disorder Cri du chat.