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Biotechnology, Part I
Biotechnology, Part I

... DNA  The gene of interest is pasted into the vector DNA along with a gene for resistance to a specific antibiotic. ...
09/06
09/06

... Ease of amplification of donor DNA fragment Convenient Restriction Sites: Single location for insertion of donor DNA ...
Gene Tagging with Transposons
Gene Tagging with Transposons

... • Transposable elements are stretches of DNA that can move to new locations in a genome • These elements can contain genes or be non-coding • Large portions of higher eukaryotes’ genomes are composed of either inert or active transposons (often as repetitive DNA) • Transposons are thus important evo ...
The viriosphere, diversity, and genetic exchange within phage
The viriosphere, diversity, and genetic exchange within phage

... that represent a small subset of marine viruses. However, because these studies target genes that are known to be present in cultured phages, they cannot address the question of the overall diversity of phage communities. Quantification of the genetic diversity of the entire viral community can be a ...
HGNC future plans
HGNC future plans

... Curate more human genes into family sets based on shared characteristics, in consultation with specialist advisors when appropriate, continue to collaborate with FlyBase about their ‘Gene Groups’. ...
Document
Document

... UCSC genome browser as data repository • The genome browser is the front-end of a data repository • The backend is a database that contains all the details about the displayed information • The information in the databases can be retrieved seperately from the download section ...
8.2 All Genetic Information Is Encoded in the Structure of DNA
8.2 All Genetic Information Is Encoded in the Structure of DNA

... • The double helix • Hydrogen bond and base pairing • Antiparallel complementary DNA strands ...
DNA ANALYSIS - Simulating Recombination
DNA ANALYSIS - Simulating Recombination

... marked area. Repeat this step for each enzyme card. Some enzyme sequences may not have a corresponding sequence on the plasmid, and that some enzyme sequences may have more than one corresponding sequence on the plasmid. In this step, you are simulating the process of choosing the correct restrictio ...
genome_map.pdf
genome_map.pdf

... National Center for Biotechnology Information One of the more extensive collections of databases is maintained by NCBI, the National Center for Biotechnology Information. NCBI was established in 1988 and is funded by the National Institutes of Health. It hosts GenBank, a public database of gene sequ ...
Document
Document

...  Making pairwise comparisons takes time  Use BLAST to rapidly select your sequences • More than 70% identity for DNA • More than 25% identity for proteins ...
Prokaryotes represent a broad group of organisms that for many
Prokaryotes represent a broad group of organisms that for many

... consider the molecular analysis described? Part B. Each group of organisms has signature sequences, regions of its SSU-rRNA (this is RNA associated with the small subunit of the ribosome) that have unique nucleotide sequences acquired by an accumulation of mutations in the ancestor of that taxonomic ...
Biology Final Review
Biology Final Review

... d. Not enough information 18. In order to be considered an evolutionarily favorable trait for an organism, a trait must increase the individual’s a. Likelihood of becoming fossilized b. Success at leaving healthy offspring c. Food intake d. Range of suitable habitats 19. Darwin observed that the Hoo ...
AP Bio DNA Sim Lab
AP Bio DNA Sim Lab

... Why is this information important? Being able to identify the precise location and sequence of human genes will allow us to better understand genetic diseases. In addition, learning about the sequence of genes in other species helps us understand evolutionary relationships among organisms. Many of o ...
Stem Cells, Cancer, and Human Health
Stem Cells, Cancer, and Human Health

... • Reproductive cloning: making animals that are genetically identical one organism with useful ...
Exercise 1: RNA
Exercise 1: RNA

... In addition to using high throughput sequencing technology for DNA sequencing, with for example the goal of discovering nucleotide variants, it can also be applied to RNA sequencing – in this case, it can be used for discovery and for quantification. RNA sequencing has the potential to also provide ...
D0SAR_BioGrid_Texas
D0SAR_BioGrid_Texas

... sequences of nucleotides composing genes Many of these sequences (a gene, part of a gene, or “junk”) are repetitive, the same sequence (or nearly the same) appearing over and over again in a chromosome or whole genome But the genomic data is huge, and genes and TEs don’t stand out David Levine ...
Why BLAST is great - GENI
Why BLAST is great - GENI

... Finding optimal alignments is computationally a very resource intensive process It is usually not necessary to find optimal alignments, particularly for large databases ...
- RNA-Seq for the Next Generation
- RNA-Seq for the Next Generation

... that the second of our analysis tools prefers lists that are not more than 500 genes. If your total list is shorter than this, you probably want to work with the complete list. To pick “interesting” genes out of the list, we need to get some additional information about each of them. A gene ontology ...
Document
Document

... aphid transmission of heteroencapsidated virions. These constructs were first expressed in Escherichia coli in order to check for the accumulation of pseudoparticles by electron microscopy. Virus-like particles (VLPs) were found with the full-length CP and with a PPV CP lacking the DAG amino acid tr ...
Photosynthesis - Mrs. Brenner's Biology
Photosynthesis - Mrs. Brenner's Biology

... • Genomics is the study of the genomes of humans and other organisms. • Sequencing the Bases  The Human Genome Project (HGP) produced a working draft of all the base pairs in all chromosomes.  Took 13 years to sequence three billion base pairs along the length of chromosomes. ...
TGAC * Sequence Polymorphisms Module
TGAC * Sequence Polymorphisms Module

... 7. Identify the red indicator for the match in the overview. (Tip: use the ruler to find the position from Q.29.) Q.30: Using the graphs in the ‘Gene_Seq’ column, determine whether PV92 is located in a gene or in the region between genes (=intergenic region). Hard to say Q.31: Approximately, at what ...
Ch 13 student notes
Ch 13 student notes

... from one organism could work in a different organism. 2. Some scientists isolated the gene from fireflies and inserted it into a plant gene. The plants glowed in the dark. 3. This showed that both plants and animals use the same process to translate DNA into proteins. 4. The glowing plant is transge ...
The Drosophila Ribosomal Protein S6 Gene Includes a 3
The Drosophila Ribosomal Protein S6 Gene Includes a 3

... resemble copy C to the left of the point of exchange but that it will be similar to copy A to the right of the point of exchange. The alignment shown in figure 2 requires the introduction of nine gaps > 1 bp to the left of an interval including the common polyadenylation signal and immediate downstr ...
ppt - Department of Plant Sciences
ppt - Department of Plant Sciences

... • Though this value is only based on probability, and therefore may not be the TRUE number of EcoRI cut sites in this genome, it can still accurately be assumed that there are A LOT of cut sites. • If restriction digested with EcoRI, the arabidopsis genome would be cut into tens of thousands of piec ...
transcription factor binding site
transcription factor binding site

... that platform; Mapping and Assembly with Qualities (MAQ)58, a widely used aligner with a more exhaustive algorithm and excellent capabilities for detecting SNPs; and Bowtie59, an extremely fast mapper that is based on an algorithm that was originally developed for file compression. These methods use ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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