HGMD® : Human Gene Mutation Database Example Queries for use
... More Specific SQL Queries in the HGMD® Professional MySQL database Get all mutations with more than one primary reference (extra references) with a pre-selected number of output fields such as mutation accession number, PMIDs from the primary and the extra references, disease name, gene symbol, and ...
... More Specific SQL Queries in the HGMD® Professional MySQL database Get all mutations with more than one primary reference (extra references) with a pre-selected number of output fields such as mutation accession number, PMIDs from the primary and the extra references, disease name, gene symbol, and ...
ProdoNet: identification and visualization of prokaryotic gene
... For the functional exploration of a particular gene of the network, a gene context menu provides the full name of the corresponding protein and links to the PRODORIC and UniProtKB databases. In case of a gene coding for an enzyme, the menu offers the ‘metabolic pathways’ option, leading to a table th ...
... For the functional exploration of a particular gene of the network, a gene context menu provides the full name of the corresponding protein and links to the PRODORIC and UniProtKB databases. In case of a gene coding for an enzyme, the menu offers the ‘metabolic pathways’ option, leading to a table th ...
2000 Genome Biology paper
... Whole-genome proteome alignments. Plots show the chromosome locations of pairs of predicted proteins that have significant similarity (on the basis of fasta3 comparisons). (a) V. cholerae chrI versus E. coli. All significant matches for each V. cholerae ORF are shown. (b) V. cholerae chrI versus E. ...
... Whole-genome proteome alignments. Plots show the chromosome locations of pairs of predicted proteins that have significant similarity (on the basis of fasta3 comparisons). (a) V. cholerae chrI versus E. coli. All significant matches for each V. cholerae ORF are shown. (b) V. cholerae chrI versus E. ...
Detection of a minor contributor in a DNA sample mixture
... Amplification products were separated and detected using the ABI PRISMR 3100 Genetic Analyzer and analyzed with GeneScanR and GenotyperR software (Applied Biosystems). 3. Results and discussion We investigated different DNA extraction methods for purification of DNA from whole or fractionated human ...
... Amplification products were separated and detected using the ABI PRISMR 3100 Genetic Analyzer and analyzed with GeneScanR and GenotyperR software (Applied Biosystems). 3. Results and discussion We investigated different DNA extraction methods for purification of DNA from whole or fractionated human ...
- fiveless|notes
... In the long term, the understanding may lead to significant advances in their management / treatment. In Evolution and Anthropology Provide insights into evolutionary relationships among various living things In Forensics DNA sequencing technologies will allow more RFLP loci to be studied, for ...
... In the long term, the understanding may lead to significant advances in their management / treatment. In Evolution and Anthropology Provide insights into evolutionary relationships among various living things In Forensics DNA sequencing technologies will allow more RFLP loci to be studied, for ...
Molecular identification of rapidly growing mycobacteria
... (Daley and Griffith, 2002). RGM identification at species level is necessary because it provides the first indication regarding the mycobacteria antibiotic susceptibility. Identification of these organisms by biochemical methods is not always a straightforward procedure (Brown-Elliott and Wallace, 2 ...
... (Daley and Griffith, 2002). RGM identification at species level is necessary because it provides the first indication regarding the mycobacteria antibiotic susceptibility. Identification of these organisms by biochemical methods is not always a straightforward procedure (Brown-Elliott and Wallace, 2 ...
Ways to detect unique sequences within mammalian DNA
... Variation in sequences between individuals is most pronounced in DNA that does not code for proteins Hypervariable regions called “polymorphic sites” Polymorphic sites - variation due to small insertions, deletions, or point mutations in restriction sites SO .. Restriction fragment with a polymorphi ...
... Variation in sequences between individuals is most pronounced in DNA that does not code for proteins Hypervariable regions called “polymorphic sites” Polymorphic sites - variation due to small insertions, deletions, or point mutations in restriction sites SO .. Restriction fragment with a polymorphi ...
幻灯片 1 - TUST
... Both single- and double-stranded phage vectors have been employed in recombinant DNA technology. For example, lambda phage derivatives are very useful for cloning and can carry fragments up to about 45 kb in length. The genes for lysogeny and integration often are nonfunctional and may be deleted to ...
... Both single- and double-stranded phage vectors have been employed in recombinant DNA technology. For example, lambda phage derivatives are very useful for cloning and can carry fragments up to about 45 kb in length. The genes for lysogeny and integration often are nonfunctional and may be deleted to ...
DNA Transcription All#read
... The terms "strong" and "weak" are often used to describe promoters and enhancers, according to their effects on transcription rates and thereby on gene expression. Alteration of promoter strength can have deleterious effects upon a cell, often resulting in disease. For example, some tumor-promoting ...
... The terms "strong" and "weak" are often used to describe promoters and enhancers, according to their effects on transcription rates and thereby on gene expression. Alteration of promoter strength can have deleterious effects upon a cell, often resulting in disease. For example, some tumor-promoting ...
Supplementary Methods, Figures and Tables This file contains
... Choice of pairs of isolates suitable for quantitative molecular analyses A major constraint in choosing isolates out of the pool of 18 was to choose pairs that could be distinguished by quantifying a small number of the 13 possible molecular markers. Only a very small number of the markers can be u ...
... Choice of pairs of isolates suitable for quantitative molecular analyses A major constraint in choosing isolates out of the pool of 18 was to choose pairs that could be distinguished by quantifying a small number of the 13 possible molecular markers. Only a very small number of the markers can be u ...
Real-time PCR Statistics - Gene
... A PCR reaction has three phases: exponential, linear, and plateau, and real-time PCR analyzes the relative abundance of PCR products during the exponential phase, in which reagents are not limited (Gibson et al., 1996; Heid et al., 1996). During the exponential phase, PCR product will ideally double ...
... A PCR reaction has three phases: exponential, linear, and plateau, and real-time PCR analyzes the relative abundance of PCR products during the exponential phase, in which reagents are not limited (Gibson et al., 1996; Heid et al., 1996). During the exponential phase, PCR product will ideally double ...
PowerPoint Presentation - Chapter 20 DNA Technology and
... The mRNA is enzymatically digested, and a second DNA strand complementary to the first is synthesized by DNA polymerase. This double-stranded DNA, called complementary DNA (cDNA), is modified by the addition of restriction sites at each end. Finally, the cDNA is inserted into vector DNA. A cDN ...
... The mRNA is enzymatically digested, and a second DNA strand complementary to the first is synthesized by DNA polymerase. This double-stranded DNA, called complementary DNA (cDNA), is modified by the addition of restriction sites at each end. Finally, the cDNA is inserted into vector DNA. A cDN ...
Human Identity Testing
... colorless. To highlight the fragments we must “bathe” the medium in probes– single-stranded DNA that is complementary to the strands used in the PCR and that carries a “lightbulb.” The lightbulb is an analogy for a radioactive label or fluorescent dye that allows it to be visible. The probe is allow ...
... colorless. To highlight the fragments we must “bathe” the medium in probes– single-stranded DNA that is complementary to the strands used in the PCR and that carries a “lightbulb.” The lightbulb is an analogy for a radioactive label or fluorescent dye that allows it to be visible. The probe is allow ...
DNA Questions #1
... 7) Fill in. There are two types of DNA found in your cells. The first type, ___nDNA__________ is found in the nucleus of your cells. It is in the form called ___chromosomes________, which are linear structures that are made of DNA and proteins. Each of your cells has ____46______(number) pieces of ...
... 7) Fill in. There are two types of DNA found in your cells. The first type, ___nDNA__________ is found in the nucleus of your cells. It is in the form called ___chromosomes________, which are linear structures that are made of DNA and proteins. Each of your cells has ____46______(number) pieces of ...
Test Info Sheet
... Sequencing of the ITGB4, ITGA6, and PLEC1 genes are offered as separate tests, usually performed in sequential order, starting with ITGB4. Using genomic DNA obtained from the submitted biological material, bidirectional sequence of the coding region and splice junctions of the ITGB4 gene (41 coding ...
... Sequencing of the ITGB4, ITGA6, and PLEC1 genes are offered as separate tests, usually performed in sequential order, starting with ITGB4. Using genomic DNA obtained from the submitted biological material, bidirectional sequence of the coding region and splice junctions of the ITGB4 gene (41 coding ...
Additional file - Supplementary material
... gene being chosen by its corresponding value on the PWF found earlier. This procedure is repeated many times and the number of times each GO category is found in our random sample is tallied. A p-value for over-representation of a category is then given by x+1/S+1, where x is the number of samples w ...
... gene being chosen by its corresponding value on the PWF found earlier. This procedure is repeated many times and the number of times each GO category is found in our random sample is tallied. A p-value for over-representation of a category is then given by x+1/S+1, where x is the number of samples w ...
DNA 1. Evidence for DNA as the genetic material.
... bacteria by viruses. i. In 1952, Alfred Hershey and Martha Chase showed that DNA was the genetic material of a bacteriophage. They used radioactive S to label the protein of the bacteriophage and allowed it to infect the host bacterium. After centrifugation, the radioactive protein was found in the ...
... bacteria by viruses. i. In 1952, Alfred Hershey and Martha Chase showed that DNA was the genetic material of a bacteriophage. They used radioactive S to label the protein of the bacteriophage and allowed it to infect the host bacterium. After centrifugation, the radioactive protein was found in the ...
7. APPLICATIONS - UTH e
... Microsatellite DNA Methodology Microsatellites (sometimes referred to as a variable number of tandem repeats or VNTRs) are short segments of DNA that have a repeated sequence such as CACACACA, and they tend to occur in non-coding DNA. In some microsatellites, the repeated unit (e.g. CA) may occu ...
... Microsatellite DNA Methodology Microsatellites (sometimes referred to as a variable number of tandem repeats or VNTRs) are short segments of DNA that have a repeated sequence such as CACACACA, and they tend to occur in non-coding DNA. In some microsatellites, the repeated unit (e.g. CA) may occu ...
Populus - University of Washington
... • Most species and hybrids can be propagated from cuttings (clones) • Controlled pollination is easy; poplars are prolific ...
... • Most species and hybrids can be propagated from cuttings (clones) • Controlled pollination is easy; poplars are prolific ...
Restriction enzymes Restriction endonucleases
... changes, or any other characteristic which can distinguish transformed hosts from untransformed hosts. ...
... changes, or any other characteristic which can distinguish transformed hosts from untransformed hosts. ...
Mitochondriontoplastid DNA transfer: it happens
... and leaving open the possibility that it began in the nuclear compartment and was then transferred independently to the mitochondrial and plastid genomes (Iorizzo et al., 2012a). Moreover, within the carrot mitochondrial genome, DcMP is fragmented and scrambled into three pieces, two of which are 80 ...
... and leaving open the possibility that it began in the nuclear compartment and was then transferred independently to the mitochondrial and plastid genomes (Iorizzo et al., 2012a). Moreover, within the carrot mitochondrial genome, DcMP is fragmented and scrambled into three pieces, two of which are 80 ...
ISMB2008PosterManagingGenomicData
... What's New in GMOD? New Components MAKER is an annotation pipeline for eukaryotic genomes that produces GMOD compatible databases. MAKER identifies repeats, aligns ESTs and proteins to a genome and produces ab initio gene predictions. The Community Annotation System (CAS) is a set of popular GMOD co ...
... What's New in GMOD? New Components MAKER is an annotation pipeline for eukaryotic genomes that produces GMOD compatible databases. MAKER identifies repeats, aligns ESTs and proteins to a genome and produces ab initio gene predictions. The Community Annotation System (CAS) is a set of popular GMOD co ...
File S1.
... described previously. Parameter values were set to allow minimum average coverage of 3, a minimum number of reads of 6 and a maximum gap of 3nt. Additionally regions had to be at least 150nt and 40nt in length for large and middle region respectively to be considered a single transcript. The filter ...
... described previously. Parameter values were set to allow minimum average coverage of 3, a minimum number of reads of 6 and a maximum gap of 3nt. Additionally regions had to be at least 150nt and 40nt in length for large and middle region respectively to be considered a single transcript. The filter ...
et al - International Journal of Systematic and Evolutionary
... present that can distinguish this group from all other bacteria. In this work, three conserved indels (i.e. inserts or deletions) are described in three widely distributed proteins that are distinctive characteristics of the Actinobacteria and are not found in any other groups of bacteria. The ident ...
... present that can distinguish this group from all other bacteria. In this work, three conserved indels (i.e. inserts or deletions) are described in three widely distributed proteins that are distinctive characteristics of the Actinobacteria and are not found in any other groups of bacteria. The ident ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.