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Biology 155 Practice Exam 3 Name
Biology 155 Practice Exam 3 Name

... 28. If you were to allow a culture of bacteria to replicate for many generations in a medium containing heavy nitrogen (15N) and then transferred a sample of your culture to a medium containing light nitrogen (14N) and allowed the cells to replicate their DNA exactly 2 times, what proportion of the ...
Yeast, Flies, Worms, and Fish
Yeast, Flies, Worms, and Fish

... a powerful approach for identifying their ortho- overexpression of the gene for a-synuclein, which has been implicated in the human disease, causes logues involved in human diseases. degenerative changes in dopaminergic neurons and abnormalities in movement.40 A model of earlydefining cellular pathw ...
qatar genome programme improves quality of genomic data
qatar genome programme improves quality of genomic data

The Genetics of Viruses and Prokaryotes The Genetics of Viruses
The Genetics of Viruses and Prokaryotes The Genetics of Viruses

... • The first to describe viruses was Beijerinck (1898), a Dutch microbial ecologist who showed that they were not killed by alcohol, did not grow on any media, and only reproduced inside a host. ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses

... DNA packing in eukaryotes. Fig. 19.2 a. DNA double helix b. Nucleosomes: DNA (-) + histone proteins (+) = “beads on a string.” c. 30-nm fiber: nucleosomes coil to form chromatin fiber d. 300-nm fiber: looped domains: loops attached to nonhistone protein scaffold. May attach to nuclear lamina for org ...
12.6 DNA Repair
12.6 DNA Repair

... kink the DNA. Pyrimidine dimers - bonds between C’s and/or T’s on the same strand.  Photolyases - enzymes that absorb light energy and use it to detect and bind to pyrimidine dimers, then break the extra ...
Genetic polymorphisms in cytochrome C oxidase subunit
Genetic polymorphisms in cytochrome C oxidase subunit

... The COI gene already has been used in forensic entomology [12, 13] and also used as a marker for species identification of bird [14], fish [15], primates [16] and also some of the insects [17]. DNA barcoding using the mitochondrial cytochrome C oxidase subunit I is a reliable method for species iden ...
Clinical Next Generation Sequencing (From Bench to Clinitions)
Clinical Next Generation Sequencing (From Bench to Clinitions)

The Promise of Pharmacogenomics
The Promise of Pharmacogenomics

... given chromosome. The more DNA markers there are on a genetic map, the more likely it is that one will be closely linked to a disease gene and made easier to target. Mapping has already been used successfully to find the single genes for several diseases, such as cystic fibrosis and muscular dystrop ...
Escherichia coli
Escherichia coli

... 1. Distinguish between the terms ‘mutation' and ‘recombination', and define the various terms that are used to identify different types of mutation 2. Describe, with specific examples, how mutations are caused by spontaneous errors in replication and by chemical and physical mutagens 3. Recount, wit ...
Viral particles
Viral particles

... T4 binds to LPS in OM (recognition by tail fibers) 169 kd linear dsDNA is injected into cell from head by tail Capsid remains outside cell 200 phage in 25! lytic cycle Genomes of T2, T4, and T6 are 85% identical, with differences accounting for binding different host receptors ...
Using DNA to Classify Life
Using DNA to Classify Life

... biologist might compare the structure of forelimbs of mammals. In recent years, biologists have also been able to compare the DNA and thus proteins in different organisms. A hypothesis known as the molecular clock hypothesis uses the comparison of DNA sequences to make predictions about the relatedn ...
Genes and causation
Genes and causation

... amino acids in proteins, but not in explaining phenotype inheritance. Whether we start from DNA or protein sequences, the question is still there. It lies in the complexity of the way in which the DNA and proteins are used by the organism to generate the phenotype. Life is not a soup of proteins. Th ...
Terauchi, R., Abe, A., Takagi, H., Tamiru, M
Terauchi, R., Abe, A., Takagi, H., Tamiru, M

... markers” to test their association with the phenotype. Following identification of genetic markers that show association with a phenotype, we explore their vicinity to identify the very genetic change that is responsible for the phenotypic variation. Two major approaches have been largely employed i ...
Molecular diagnosis and inborn errors of metabolism
Molecular diagnosis and inborn errors of metabolism

... too cost-inefficient to justify routine clinical use, at least under the constraint currently extant in U.S. medicine. The use of DNA analysis has been particularly prevalent in ~ o s s i b l edisorders of energy metabolism, but here too, as the technologies expand, the efficiency will continue to f ...
Enzymes - year13bio
Enzymes - year13bio

... If damage occurs in either of the 2 genes mentioned above the cell will grow at an uncontrolled rate, or become effectively immortal. These cells cease to carry out normal functioning. If the damage is not too severe the cells may form a benign tumour. If many genes are affected the tumour is said t ...
Comparative Genomic Hybridization
Comparative Genomic Hybridization

Mutation PPT
Mutation PPT

... • Remember that DNA is made up of four nucleotide bases: A, T, G, C • Each gene is a string of hundreds of base pairs in a particular sequence. • An allele is one variant of that instruction. ...
introduction_to_micr..
introduction_to_micr..

Dangerously Thin: A case study on the Genetic Code
Dangerously Thin: A case study on the Genetic Code

... Through genetic testing, Henry was found to carry a mutation in a gene for an enzyme called CYP2C9. While the strange name of the gene does not really fully appear to capture the importance of its function, it has a role in breaking down more than 15% of the drugs currently in use, and as many as 35 ...
PowerPoint Lecture Chapter 9
PowerPoint Lecture Chapter 9

... 2. Still to small to see or work with directly a. Scientist work with DNA without being able to handle it directly b. Use Chemicals, computers, and bacteria as tools to study DNA ...
lecture_ch05_2014 honors biology_website
lecture_ch05_2014 honors biology_website

... Insert figure 5-45c ...
Organisation of the human genome and our tools for
Organisation of the human genome and our tools for

... is processed from DNA through another polymer, ribonucleic acid (RNA), into polypeptides that form the basic components of all proteins (Fig. 1). In most somatic cells of the body the human genome contains 3000 million basepairs arranged in 22 pairs of chromosomes (autosomes) and a pair of X chromos ...
Chapter 14 Transposons, Plasmids, and Bacteriophage
Chapter 14 Transposons, Plasmids, and Bacteriophage

... – not by transposase but by resolvase (product of tapR gene) – Recombination step (resolution) occurs at IRS sites in paired copies in cointegrate called res ...
Study Guide
Study Guide

... A double stranded DNA molecule is broken by helicase, which separates the two strands by  breaking the weak hydrogen bonds that links the nitrogenous bases together. Next, DNA  polymerase adds complementary nucleotides to each strand of DNA. The end product is two  identical copies of double strande ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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