• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Zebrafish - yourgenome
Zebrafish - yourgenome

... What is a model organism? • Non-human species widely studied to understand human disease. • Model organisms are used when experimentation using humans is unfeasible or ...
Molecular and Genomics-Based Diagnostics for Medical Microbiology
Molecular and Genomics-Based Diagnostics for Medical Microbiology

... A facilitated and rapid access of clinical microbiology laboratories to sequencing platforms A need for standardized and fully automated sequence interpretation that would ideally be independent of both the sequencing platform and the exact species of microorganism The ability to translate the data ...
Gene Trees, Populations and the Microbial Species Concept
Gene Trees, Populations and the Microbial Species Concept

... • Allows for exchange of auxiliary genes • Predicts that core genes will show higher levels of recombination within a species than between species • Predicts that core genes will diverge more rapidly than auxiliary genes between species ...
supplementary materials
supplementary materials

... replacement using CgURA3 PCR product [3]. Insertion was tested by PCR. GFP-TRP1 was inserted into the SPO77 locus of yEJ129 by one-step recombination using GFP and TRP1 amplified by PCR from plasmid pFA6a-GFP(S65T)-TRP1 to make strain yEJ152 and tested by PCR [4]. All in vivo gene and element replac ...
DNA Structure, Function and Replication – Teacher Notes
DNA Structure, Function and Replication – Teacher Notes

Milestone1
Milestone1

... Proteins are linear chains of amino acid residues that fold into complex 3D structures that carry out cellular functions. Proteins that have similar linear sequences of amino acid residues often fold into similar 3D shapes and have similar functions. In lecture, we discussed the term “homology” in t ...
T - Flushing Community Schools
T - Flushing Community Schools

... = the process by which the number of chromosomes is reduced by half to form sex cells (eggs and sperm) n  See ...
Linköping University Post Print β-tubulin mutations in ovarian cancer using
Linköping University Post Print β-tubulin mutations in ovarian cancer using

... Ovarian cancer is a common malignancy in women and chemotherapy plays an important role in the treatment following the initial surgery. A major clinical advance was made in the early 1990s when paclitaxel (Taxol ) in combination with a platinum derivative was introduced in the treatment of ovarian c ...
BW 180-182
BW 180-182

... CHECK SHEET ASSIGNMENT ...
BIOL 112 – Principles of Zoology
BIOL 112 – Principles of Zoology

... 6. Double strand break repair Repairs DSBs by reannealing the two DNA segments - protein aligns the broken ends of DNA for rejoining Recombination repair mechanism ...
chapter 20
chapter 20

... This creates a diverse pool of bacteria: some bacteria that have taken up the desired recombinant plasmid DNA, and other bacteria that have taken up other DNA, both recombinant and nonrecombinant. 5. The transformed bacteria are plated on a solid nutrient medium containing ampicillin and a molecular ...
class syllabus
class syllabus

... hearing, and she decides to mate her dog to Lukas’ dog. Among the resulting litter of 8 puppies (litter 1), four have normal hearing, and four are deaf. Sophie chooses two of the puppies with normal hearing from litter 1, allows them to grow up, and then mates them to one another to produce a litter ...
4. - UKZN Management Information
4. - UKZN Management Information

... alanine ...
human gene testing - National Academy of Sciences
human gene testing - National Academy of Sciences

... with the aid of another type of enzyme, called ligase. By 1973, researchers were using restriction enzymes to cut specific DNA sequences of interest and join them to the DNA of bacteria. The bacteria then generated copies of the selected DNA with their own DNA each time they divided. Because a singl ...
Microbial Genetics Lab
Microbial Genetics Lab

... covered by high-density probes. These allow the identification of transcription start sites and operons and thus regions important in gene regulation. In this experiment we would isolate RNAs from a control cyanobacterium and a mutant cyanobacterium, e.g. one with a defect in photosynthetic electron ...
1989 Allen Award Address: The American Society of Human Genetics Annual Meeting, Baltimore.
1989 Allen Award Address: The American Society of Human Genetics Annual Meeting, Baltimore.

... You have done Ray White and me a great honor in selecting us for the Allen Award this year, and I want first of all to express my appreciation of it. Even though I received my Ph.D. from the Human Genetics Department at the University of Michigan, I never imagined that I would contribute directly to ...
designer genes * southern poly regional 2006
designer genes * southern poly regional 2006

... If the rate of transcription is 50 bases/sec, approximately how long will it take the cell to make the RNA for dystrophin? A. ...
Gene testing - Margie Patlak
Gene testing - Margie Patlak

... with the aid of another type of enzyme, called ligase. By 1973, researchers were using restriction enzymes to cut specific DNA sequences of interest and join them to the DNA of bacteria. The bacteria then generated copies of the selected DNA with their own DNA each time they divided. Because a singl ...
Linkage, Recombination, and Crossing Over
Linkage, Recombination, and Crossing Over

... bp amplified region there must be a DNA sequence difference (polymorphism) between individuals. • The most common DNA marker systems examine the number of repeated units in a simple sequence repeat motif, such as CACACACACACACAC. • Individuals can vary considerably in the number of CA blocks, making ...
Next generation sequencing
Next generation sequencing

... Depiction of a new type of highly miniaturized microarray that incorporates randomness in its design. Each array contains ~50,000 beads carrying oligonucleotide probes. The beads are lodged in wells on the surface of a hexagonally packed optical fiber bundle. The location and identity of the random ...
Dharmacon Solutions for Studying Gene Function
Dharmacon Solutions for Studying Gene Function

... Analyzer 2200, IN Cell Analyzer 6000 or Cytell™ Cell Imaging System. These cutting-edge quantitative imaging technologies give you incredible depth and breadth of investigation, allowing you to analyze interactions as they happen and gain deeper insights into complex cellular mechanisms. The high-th ...
Final project
Final project

... parameters but with a requirement of getting at least 2000bp PCR product. a. How many primer pairs did you find, and what is the length of the product for each pair? b. Translate this gene into a protein and run it against the PFAM database. List the protein domains you found from the PFAM search? Q ...
File
File

... Each triplet codes for one amino acid / single amino acid may have up to 6 different triplets for it due to the redundancy of the code / code is degenerate. Some amino acids are coded by more than one codon Same triplet code will give the same amino acid in virtually all organisms, universal code We ...
Chapter 13 DNA - Pearson Places
Chapter 13 DNA - Pearson Places

... Because nitrogen base pairing ensures that the opposite polymer strand is produced from each half of the double helix Q13. How are DNA databases useful for forensic analysis? A13. To eliminate individual from suspicion; to identify the culprit of a crime, to identify victims of a natural disaster or ...
Human Genetic Engineering
Human Genetic Engineering

... transfer the normal gene into only those tissues most affected by the disease. For instance, bronchial infections are the major cause of early death for patients with cystic fibrosis (CF). The lungs of CF patients produce thick mucus that provides a great growth medium for bacteria and viruses. If t ...
< 1 ... 182 183 184 185 186 187 188 189 190 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report