Slide 1 - Montville.net
... recombinant plasmid, a plasmid with a new gene inserted. The plasmid will contain DNA from two different organisms. You will use colored paper, scissors and tape to do this. If you are successful, you will have a two colored paper ring and extra pieces of paper. ...
... recombinant plasmid, a plasmid with a new gene inserted. The plasmid will contain DNA from two different organisms. You will use colored paper, scissors and tape to do this. If you are successful, you will have a two colored paper ring and extra pieces of paper. ...
MUTATIONS • Mutations are errors made in the DNA sequence that
... nonsense mutation involves a change in the DNA sequence that causes a stop codon to replace a codon for an aa; only the part of the protein prior to the stop codon will be produced. they are often lethal to the cell. ...
... nonsense mutation involves a change in the DNA sequence that causes a stop codon to replace a codon for an aa; only the part of the protein prior to the stop codon will be produced. they are often lethal to the cell. ...
Bacterial Transformation - Eastern Regional High School
... bacteria by uptake of foreign/new DNA ...
... bacteria by uptake of foreign/new DNA ...
BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 2 Ch 4, 11
... 24. To explain the mechanism of transformation and view aspects of plasmids including ori, ampr, plasmid size, extrachromosomal maintance, and the multiple cloning sites for the insertion of foreign genes 25. To examine the pGLO plasmid, ori, ampr ,the GFP gene, and the portion of the arabinose prom ...
... 24. To explain the mechanism of transformation and view aspects of plasmids including ori, ampr, plasmid size, extrachromosomal maintance, and the multiple cloning sites for the insertion of foreign genes 25. To examine the pGLO plasmid, ori, ampr ,the GFP gene, and the portion of the arabinose prom ...
Mark scheme - biologypost
... Shows which cells/bacteria have taken up the plasmid / which cells/bacteria have taken up the gene; Only modified cells/bacteria / cells/bacteria with the plasmid survive in presence of the antibiotic/are resistant (to antibiotic); ...
... Shows which cells/bacteria have taken up the plasmid / which cells/bacteria have taken up the gene; Only modified cells/bacteria / cells/bacteria with the plasmid survive in presence of the antibiotic/are resistant (to antibiotic); ...
Key
... Derive clones of ES cells with gene replacement. Inject cells into blastocysts from a black mouse. Implant blastocysts into a pseudopregnant mother. Allow embryos to develop to term and identify chimeric mice. Breed chimeric mice to establish heterozygotes. Interbreed heterozygotes to obtain homozyg ...
... Derive clones of ES cells with gene replacement. Inject cells into blastocysts from a black mouse. Implant blastocysts into a pseudopregnant mother. Allow embryos to develop to term and identify chimeric mice. Breed chimeric mice to establish heterozygotes. Interbreed heterozygotes to obtain homozyg ...
+ IPTG + X-gal
... and -Complementation 1. Cut out gene of interest with restriction enzyme 2. Cut B/W cloning vector with same restriction enzyme (MCS) a. Dephosphorylate vector to prevent self-ligation 3. Mix insert with vector and add ligase 4. Transform E. coli that is made for B/W screening 5. Plate onto media t ...
... and -Complementation 1. Cut out gene of interest with restriction enzyme 2. Cut B/W cloning vector with same restriction enzyme (MCS) a. Dephosphorylate vector to prevent self-ligation 3. Mix insert with vector and add ligase 4. Transform E. coli that is made for B/W screening 5. Plate onto media t ...
Must Knows - Gene Regulation and Biotechnology
... 6) Let’s say methyl groups are added to the DNA of the gene coding for human growth hormone. How will this affect the amount of human growth hormone produced? 7) Let’s say acetyl groups are added to the histone proteins that interact wth the DNA of the gene coding for human growth hormone. How will ...
... 6) Let’s say methyl groups are added to the DNA of the gene coding for human growth hormone. How will this affect the amount of human growth hormone produced? 7) Let’s say acetyl groups are added to the histone proteins that interact wth the DNA of the gene coding for human growth hormone. How will ...
DNA, Genes and Chromosomes
... model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled during sexual reproduction. ...
... model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled during sexual reproduction. ...
Ligation and Transformation
... 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
... 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection media ...
Genes, Chromosomes and DNA
... _________ is found in the _________ of each of the body's billions of cells. Every human cell (with the exception of mature red blood cells, which have no nucleus) contains the same _________. Each cell has 46 molecules of doublestranded DNA. Each molecule of DNA is made up of 50 to 250 million base ...
... _________ is found in the _________ of each of the body's billions of cells. Every human cell (with the exception of mature red blood cells, which have no nucleus) contains the same _________. Each cell has 46 molecules of doublestranded DNA. Each molecule of DNA is made up of 50 to 250 million base ...
Honors Biology
... 3. Know the types and roles of RNAs. 4. Compare and contrast RNA with DNA (consider both the structure of each and the purpose of each in the cell). 5. Describe the process of protein synthesis (both transcription and translation). 6. Explain the steps of mRNA processing and how it can result in dif ...
... 3. Know the types and roles of RNAs. 4. Compare and contrast RNA with DNA (consider both the structure of each and the purpose of each in the cell). 5. Describe the process of protein synthesis (both transcription and translation). 6. Explain the steps of mRNA processing and how it can result in dif ...
Genetic Control of Metabolism
... increase the yield of desired product. • The ability to secrete product into the surrounding medium. This allows it to be collected easily, saving resources. • Ensuring it can’t survive in the external environment. This is a safety ...
... increase the yield of desired product. • The ability to secrete product into the surrounding medium. This allows it to be collected easily, saving resources. • Ensuring it can’t survive in the external environment. This is a safety ...
As Powerpoint Slide
... heterozygous background in mice. For MADM, two reciprocal chimeric marker genes – GT and TG – are targeted separately to identical loci on homologous chromosomes. Following recombinase-mediated interchromosomal recombination, functional green and red fluorescent proteins are reconstituted. If recomb ...
... heterozygous background in mice. For MADM, two reciprocal chimeric marker genes – GT and TG – are targeted separately to identical loci on homologous chromosomes. Following recombinase-mediated interchromosomal recombination, functional green and red fluorescent proteins are reconstituted. If recomb ...
Bacterial Genetic
... bacterium towards it creating a mating bridge…serves as the avenue for DNA transfer • There needs to be a “fertility” (F) gene present either as part of the bacterial genome or as a plasmid…an F plasmid is an episome: genetic element that can replicate independently or as part of the bacterial gen ...
... bacterium towards it creating a mating bridge…serves as the avenue for DNA transfer • There needs to be a “fertility” (F) gene present either as part of the bacterial genome or as a plasmid…an F plasmid is an episome: genetic element that can replicate independently or as part of the bacterial gen ...
Chapter 12 SWBAT`s and Standards
... Genes are a set of instructions encoded in the DNA sequence of each organism that specify the sequence of amino acids in proteins characteristic of that organism. As a basis for understanding this concept: a. ...
... Genes are a set of instructions encoded in the DNA sequence of each organism that specify the sequence of amino acids in proteins characteristic of that organism. As a basis for understanding this concept: a. ...
electroporation of a - The Steve Clough Lab
... simultaneously pressing both red buttoms on face of gene pulser. Pulser will beep when finished. Time reading ideally will be above 9.3, but lower values may still be ok. Time will be lower the more salt (remember that DNA is also a salt) present in solution and electoporation efficiency may be redu ...
... simultaneously pressing both red buttoms on face of gene pulser. Pulser will beep when finished. Time reading ideally will be above 9.3, but lower values may still be ok. Time will be lower the more salt (remember that DNA is also a salt) present in solution and electoporation efficiency may be redu ...
Supplementary Information (doc 83K)
... The reporter plasmid pBIO1878 was made by cloning a 2 kb BamHI SpcR cassette fragment from plasmid pHP45 (Prentki and Krisch, 1984) into the BglII site of pMP220, which is a wide hostrange promoter-probe plasmid with a lacZ gene lacking its native promoter (Spaink et al., 1987). The region of the R ...
... The reporter plasmid pBIO1878 was made by cloning a 2 kb BamHI SpcR cassette fragment from plasmid pHP45 (Prentki and Krisch, 1984) into the BglII site of pMP220, which is a wide hostrange promoter-probe plasmid with a lacZ gene lacking its native promoter (Spaink et al., 1987). The region of the R ...
Genes and Mutations 1. Define: Genetics – Genetics may be defined
... with the physical and chemical properties of the genetic material (DNA or RNA), how this material is transmitted from one generation to the next, and how the information it contains is expressed. Phenotype – The phenotype of an organism is the observed characteristics of that organism or in the case ...
... with the physical and chemical properties of the genetic material (DNA or RNA), how this material is transmitted from one generation to the next, and how the information it contains is expressed. Phenotype – The phenotype of an organism is the observed characteristics of that organism or in the case ...