Plasmids by Dr. Ty C.M. Hoffman
... gene for resistance to the antibiotic, ampicillin, a transformed cell will survive on a plate containing ampicillin, but a non-‐transformed cell will not survive. The pGLO plasmid is so named, because the ...
... gene for resistance to the antibiotic, ampicillin, a transformed cell will survive on a plate containing ampicillin, but a non-‐transformed cell will not survive. The pGLO plasmid is so named, because the ...
Me oh Mi!
... What Family do humans belong to? (hint: part of the same family of an individual named after a ...
... What Family do humans belong to? (hint: part of the same family of an individual named after a ...
The Human Genome Project
... The Human Genome Project What is the Human Genome Project? • U.S. govt. project coordinated by the Department of Energy and the National Institutes of Health • goals (1998-2003) – identify the approximate 100,000 genes in human DNA – determine the sequences of the 3 billion bases that make up human ...
... The Human Genome Project What is the Human Genome Project? • U.S. govt. project coordinated by the Department of Energy and the National Institutes of Health • goals (1998-2003) – identify the approximate 100,000 genes in human DNA – determine the sequences of the 3 billion bases that make up human ...
DNA Replication, Transcription, and Translation STUDY GUIDE
... What are the main functions of DNA polymerase? The main function of tRNA is to: What is the term for a three-nucleotide sequence that codes for an amino acid? How many amino acids are used to make up the all of the proteins in the human body? A tRNA that carries the amino acid methionine pairs with ...
... What are the main functions of DNA polymerase? The main function of tRNA is to: What is the term for a three-nucleotide sequence that codes for an amino acid? How many amino acids are used to make up the all of the proteins in the human body? A tRNA that carries the amino acid methionine pairs with ...
Research Questions
... like to reside in an aqueous environment. For this reason, one generally finds these amino acids buried within the hydrophobic core of the protein, or within the lipid portion of the membrane. Hydrophilic amino acids do not like aqueous enviroments and are polar. They can also interact via ionic bon ...
... like to reside in an aqueous environment. For this reason, one generally finds these amino acids buried within the hydrophobic core of the protein, or within the lipid portion of the membrane. Hydrophilic amino acids do not like aqueous enviroments and are polar. They can also interact via ionic bon ...
ppt
... produced and passed to the daughter cells. Two kinds of DNA molecule that satisfy these criteria can be found in bacterial cells: plasmids and bacteriophage chromosomes. We concentrate in this course only on plasmids. The greatest variety of cloning vectors exist for use with E. coli as the host org ...
... produced and passed to the daughter cells. Two kinds of DNA molecule that satisfy these criteria can be found in bacterial cells: plasmids and bacteriophage chromosomes. We concentrate in this course only on plasmids. The greatest variety of cloning vectors exist for use with E. coli as the host org ...
Review for Lecture 18
... 15. A eukaryotic gene (MEK1) has "sticky ends" produced by the restriction endonuclease EcoRI. A plasmid, which contains genes for resistance to ampicillin and tetracycline (antibiotics), has one recognition site for EcoRI located in the tetracycline resistance gene. The plasmid is cut with EcoRI an ...
... 15. A eukaryotic gene (MEK1) has "sticky ends" produced by the restriction endonuclease EcoRI. A plasmid, which contains genes for resistance to ampicillin and tetracycline (antibiotics), has one recognition site for EcoRI located in the tetracycline resistance gene. The plasmid is cut with EcoRI an ...
Chloramphenicol PDF
... plasmid DNA yields can be improved by adding chloramphenicol to the culture medium. Chloramphenicol inhibits host protein synthesis and thus prevents replication of the host chromosome. Plasmid replication, however, is independent of newly synthesized proteins and continues for several hours until u ...
... plasmid DNA yields can be improved by adding chloramphenicol to the culture medium. Chloramphenicol inhibits host protein synthesis and thus prevents replication of the host chromosome. Plasmid replication, however, is independent of newly synthesized proteins and continues for several hours until u ...
Basics
... (lac-proAB): deleted for the genes from lac to proAB thi: can’t make thiamine gyrA96: a specific mutation in the gyrase A gene endA1: a specific mutation in the endA (endonuclease) gene relA1: a specific mutation in the relA gene supE44: a specific mutation that suppresses nonsense mutations – : n ...
... (lac-proAB): deleted for the genes from lac to proAB thi: can’t make thiamine gyrA96: a specific mutation in the gyrase A gene endA1: a specific mutation in the endA (endonuclease) gene relA1: a specific mutation in the relA gene supE44: a specific mutation that suppresses nonsense mutations – : n ...
Vocabulary 7
... • When one of the 4 base pairs is : –(substitution) “replaced” or –(insertion) “added” or –(deletion) “removed” ...
... • When one of the 4 base pairs is : –(substitution) “replaced” or –(insertion) “added” or –(deletion) “removed” ...
VI. Genetic Engineering or Recombinant DNA Technology
... 1. Cell walls are digested which leaves naked protoplasts 2. Protoplasts minus their walls can then fuse or hybridize 3. Hybrid cells can be selected for and cultured 4. Somatic hybrids are the result of a fusion from two different protoplasts E. Clonal Variants 1. Cells with slightly different char ...
... 1. Cell walls are digested which leaves naked protoplasts 2. Protoplasts minus their walls can then fuse or hybridize 3. Hybrid cells can be selected for and cultured 4. Somatic hybrids are the result of a fusion from two different protoplasts E. Clonal Variants 1. Cells with slightly different char ...
Virtual DNA Lab
... 7. What are genes made from? ________________________ 8. a. What is the function of the protein HEMOGLOBIN? ...
... 7. What are genes made from? ________________________ 8. a. What is the function of the protein HEMOGLOBIN? ...
PowerPoint - Land of Biology
... Made in the pancreas, allows cells to take up glucose Supplemental insulin was provided by taking it from camels and sheep That makes the sheep and camels grumpy and dead. Plus, some people were allergic or sensitive to camel and sheep proteins. ...
... Made in the pancreas, allows cells to take up glucose Supplemental insulin was provided by taking it from camels and sheep That makes the sheep and camels grumpy and dead. Plus, some people were allergic or sensitive to camel and sheep proteins. ...
Honors Biology Final Outline
... The central dogma for biological information: DNA, RNA, & Protein The DNA of a gene serves as a template for transcribing this information into RNA (base pairing) RNA must first be processed (introns, exons) in eukaryotes before a mRNA can be formed o generates transcript diversity RNA struc ...
... The central dogma for biological information: DNA, RNA, & Protein The DNA of a gene serves as a template for transcribing this information into RNA (base pairing) RNA must first be processed (introns, exons) in eukaryotes before a mRNA can be formed o generates transcript diversity RNA struc ...
B2 Topic 1 The Components of Life
... What uses are there for genetic engineering? Bacteria Cell ...
... What uses are there for genetic engineering? Bacteria Cell ...
Questions - Vanier College
... 8. A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that A) bacteria translate polycistronic messages only. B) bacteria cannot remove eukaryotic introns. C) prokaryotes use a different genetic code from that of eukaryote ...
... 8. A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that A) bacteria translate polycistronic messages only. B) bacteria cannot remove eukaryotic introns. C) prokaryotes use a different genetic code from that of eukaryote ...
Genetic Engineering
... Steps for scientists to transfer genes from one organism to another: Restriction enzymes were used naturally to cut out viral DNA from their own DNA and destroy it 1. Cut the DNA containing the gene of interest (GOI) away from the genes surrounding it ...
... Steps for scientists to transfer genes from one organism to another: Restriction enzymes were used naturally to cut out viral DNA from their own DNA and destroy it 1. Cut the DNA containing the gene of interest (GOI) away from the genes surrounding it ...
Document
... 2. ___reverse transcriptase__ is an RNA-dependent DNA polymerase. 3. Knockout mice are created by replacing a normal gene segment with a modified segment within embryonic _stem cells_______, then using the latter to create a chimeric embryo. 4. __transformation____ is a method of DNA transfer in bac ...
... 2. ___reverse transcriptase__ is an RNA-dependent DNA polymerase. 3. Knockout mice are created by replacing a normal gene segment with a modified segment within embryonic _stem cells_______, then using the latter to create a chimeric embryo. 4. __transformation____ is a method of DNA transfer in bac ...
Tigger/pogo transposons in the Fugu genome
... Another theme will be genome size, which involves a variety of effects. But we can think of two levels of analysis. First, there is the mechanistic question of why genomes get bigger or smaller. For example, they generally get bigger by accumulating many copies of pseudogenes or transposable elemen ...
... Another theme will be genome size, which involves a variety of effects. But we can think of two levels of analysis. First, there is the mechanistic question of why genomes get bigger or smaller. For example, they generally get bigger by accumulating many copies of pseudogenes or transposable elemen ...
Agriculture - eduBuzz.org
... plant using Ti cont… The foreign DNA (target gene) is then added to the plasmid and sealed using DNA ligase The modified plasmid is returned to the Agrobacterium (Note the plasmid also has a method for identification e.g antibiotic resistance – later in the process this is used to identify the ...
... plant using Ti cont… The foreign DNA (target gene) is then added to the plasmid and sealed using DNA ligase The modified plasmid is returned to the Agrobacterium (Note the plasmid also has a method for identification e.g antibiotic resistance – later in the process this is used to identify the ...
Slide 1
... during reproduction between generations of cells. Horizontal gene transfer: The transfer of genes between cells of the same generation. ...
... during reproduction between generations of cells. Horizontal gene transfer: The transfer of genes between cells of the same generation. ...
Biological ideas relating to genetic modification
... Cell division which produces sex cells. Results in four unique haploid cells. ...
... Cell division which produces sex cells. Results in four unique haploid cells. ...
F plasmid
... recognizing a stem loop formed at the nut sites. Host proteins are involved in antitermination. N causes antitermination at both r-dependent and r-independent terminators by restricting the pause time at the terminator. ...
... recognizing a stem loop formed at the nut sites. Host proteins are involved in antitermination. N causes antitermination at both r-dependent and r-independent terminators by restricting the pause time at the terminator. ...
Genetically Modified Food
... Mechanism1 : Isolating/synthesizing the target gene The ‘shotgun’ approach, using type II restriction enzyme(restriction endonuclease ) - the enzyme cuts at recognition sites, to obtain a desired gene - sticky ends or blunt ends produced (there are figures later) Making a copy of the gene from ...
... Mechanism1 : Isolating/synthesizing the target gene The ‘shotgun’ approach, using type II restriction enzyme(restriction endonuclease ) - the enzyme cuts at recognition sites, to obtain a desired gene - sticky ends or blunt ends produced (there are figures later) Making a copy of the gene from ...
Genetic and Genomics: An Introduction
... the female), each gamete may not carry the exact same DNA sequence, i.e., a polymorphism (poly = many, morph = form) may occur which involves one of two or more variants of a particular DNA sequence. The most common polymorphism involves variation at a single base pair. This variation is called a si ...
... the female), each gamete may not carry the exact same DNA sequence, i.e., a polymorphism (poly = many, morph = form) may occur which involves one of two or more variants of a particular DNA sequence. The most common polymorphism involves variation at a single base pair. This variation is called a si ...