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Stem cells - Plain Local Schools
Stem cells - Plain Local Schools

... II. Regulation of Genes in Eukaryotes A. More elaborate and complicated than in prokaryotes B. Eukaryotic DNA includes promoter sequences before the point that transcription takes place C. Transcription factors- regulate transcription by binding to promoters or RNA polymerases D. Transcription fact ...
HW3 - solutions
HW3 - solutions

... a. Explain in one-two sentences what does each ontology refer to and give one example for each. Cellular component – annotations of genes by the location of the protein they code to in the cell. For example – cell membrane. Biological process – annotations of genes by the pathway or process the prot ...
Biotechnology - Biology Junction
Biotechnology - Biology Junction

...  if you are going to engineer DNA & genes & organisms, then you need a set of tools to work with  this unit is a survey of those tools… ...
Lab 12
Lab 12

... sequences -each enzyme recognizes and cuts DNA at a different base sequence e.g. BamHI XXXXXXXXGGATCCXXXXXXXXXX XXXXXXXXCCTAGGXXXXXXXXXX -due to spontaneous mutations over time, different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in ...
Gene Expression
Gene Expression

... are coding DNA (translated into a protein), which are only about 2% of human genome  introns are non-coding DNA, which provide structural integrity and regulatory (control) functions  exons can be thought of program data, while introns provide the program logic  Humans have much more control stru ...
Nerve activates contraction
Nerve activates contraction

... • The first cloning vector is often a yeast artificial chromosome (YAC), which can carry inserted fragments up to a million base pairs long, or a bacterial artificial chromosome (BAC), which can carry inserts of 100,000 to 500,000 base pairs. • After the order of these long fragments has been determ ...
Lecture 3 - Computing for Bioinformatics I
Lecture 3 - Computing for Bioinformatics I

... same libraries and the same sets of books. • Books represent all the information (DNA) that every cell in the body needs so it can grow and carry out its various functions. ...
The Genetics of Viruses and Prokaryotes The Genetics of Viruses
The Genetics of Viruses and Prokaryotes The Genetics of Viruses

... • The first to describe viruses was Beijerinck (1898), a Dutch microbial ecologist who showed that they were not killed by alcohol, did not grow on any media, and only reproduced inside a host. ...


... extracting a significant quantity of genomic DNA, undergoing restriction digestions prior to blotting and probing. This is time consuming and often involves the use of 32P. Since its discovery, PCR has spawned a multitude of variations that have been accepted in many forms of biology and medicine. A ...
DNA, RNA and Proteins
DNA, RNA and Proteins

... specific region of DNA (a gene) is transcribed, or copied into RNA. Step 1: RNA polymerase binds to the promoter ( a specific DNA sequence/start location). Step 2: RNA polymerase unwinds the dbl helix to expose both paired nucleotide bases. Step 3: RNA polymerase links and binds complementary base u ...
94 Didn`t you notice the conversation between the grandmother and
94 Didn`t you notice the conversation between the grandmother and

... The genetic revolution that was started by Gregor Mendel in pea plants has developed to such an extent that today it influences all sections of society. The knowledge we have gained about genes and the technology that alters genetic structure are progressing every moment. Technology that is used to ...
Gene duplication and rearrangement
Gene duplication and rearrangement

... sample in different reading frames staggered by two bases ...
Did you ever get a message from a friend that was in code
Did you ever get a message from a friend that was in code

... DNA is the recipe (gene) that is found on the chromosome in the nucleus. The recipe gets to the ribosome (in the cytoplasm) by the RNA writing it down in the nucleus and bringing it to ribosomes to be made. ...
Recombinant DNA
Recombinant DNA

... – Vector – piece of DNA that foreign DNA can be added to • Plasmids are accessory rings of DNA in bacteria, commonly used as vectors. • They are not part of the bacterial chromosomes. ...
Lesson 63 Show Me the Genes KEY
Lesson 63 Show Me the Genes KEY

... 9. How are the genes and alleles alike and different? Pairs of chromosomes carry the same genes, but each chromosome may have a different version of the gene (allele). 10. Consider two children with the same two parents. Would you expect them to have the same set of chromosomes? Explain why or why n ...
general abstract
general abstract

Stem Cells, Cancer, and Human Health
Stem Cells, Cancer, and Human Health

... • Can cause mutations: – Mistakes in DNA replication – Collisions of the DNA molecule with other molecules – Damage from heat or chemical agents ...
Lec-Functional Annotation and Functional Enrichment2010
Lec-Functional Annotation and Functional Enrichment2010

... • Molecular function describes activities, such as catalytic or binding activities, that occur at the molecular level. GO molecular function terms represent activities rather than the entities (molecules or complexes) that perform the actions, and do not specify where or when, or in what context, th ...
Gene Tagging with Transposons
Gene Tagging with Transposons

... • Constructed Ty1 element with a galactose-inducible promoter and an intron • Used galactose to stimulate transcription, then found that all the new copies transposed had the intron spliced out ...
Newsletter - Malaysian Node of the Human Variome Project
Newsletter - Malaysian Node of the Human Variome Project

... transcribed into messenger RNA (mRNA) molecules and translated into proteins which carry out cell functions. The recent completion of the Human Genome Project has provided an unprecedented opportunity for researchers to identify high-risk patients and improve human health through the use of technolo ...
BPS 555
BPS 555

... Trans-acting Transcription Factors and Cis-acting regulating elements are required for Gene Expression • Short sequence elements in the vicinity of the gene (cis) are recognized by transcription factors (trans) to guide and recruit RNA polymerase. ...
Genetics - Mrs. Yu`s Science Classes
Genetics - Mrs. Yu`s Science Classes

... building blocks of ribosomes.  The nucleolus is an assemblage of DNA actively being transcribed into rRNA.  Within the nucleolus, various proteins imported from the cytoplasm are assembled with rRNA to form large and small ribosome subunits.  Together, the two subunits form a ribosome that coordi ...
Essential Question
Essential Question

... Essential Question What is DNA made of and how ...
Chapter 9
Chapter 9

... its location on a chromosome (even though the gene itself is not changed), such a variation is called “position effect” ...
DNA, RNA, and the Flow of Genetic Information
DNA, RNA, and the Flow of Genetic Information

... human genome comprises approximately 3 billion nucleotides in each chain of DNA, divided among 24 distinct molecules of DNA called chromosomes (22 autosomal chromosomes plus the X and Y sex chromosomes) of different sizes. One of the largest known DNA molecules is found in the Indian muntjac, an Asi ...
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Human genome



The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.
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