Case series reporting the effectiveness of mycophenolate mofetil in treatment- resistant asthma
... It must be recognised, however, that these positive outcomes have arisen in an open label case series. Although most of these patients had been trialled with other novel therapies, including enrolment in formal trials, and MMF appears to have had an effect where these other medications had not, a pl ...
... It must be recognised, however, that these positive outcomes have arisen in an open label case series. Although most of these patients had been trialled with other novel therapies, including enrolment in formal trials, and MMF appears to have had an effect where these other medications had not, a pl ...
Investigation of Common Variations of ABCB4, ATP8B1 and
... (19), denaturing high performance liquid chromatography (DHPLC), and DNA sequencing (20). The gold standard is DNA sequencing since it can reveal mutations and variations across three genes with 28 exons for each. However, this method is very expensive. Therefore, the current study tried to investig ...
... (19), denaturing high performance liquid chromatography (DHPLC), and DNA sequencing (20). The gold standard is DNA sequencing since it can reveal mutations and variations across three genes with 28 exons for each. However, this method is very expensive. Therefore, the current study tried to investig ...
Genetic and Genomic Influences
... Completion of the Human Genome Project in 2003 heralded the dawn of the genomic era of health care. It has long been known that some diseases occur due to specific gene defects. Genetic diseases have traditionally been thought of as inherited diseases caused by a defect in a single gene. While “typi ...
... Completion of the Human Genome Project in 2003 heralded the dawn of the genomic era of health care. It has long been known that some diseases occur due to specific gene defects. Genetic diseases have traditionally been thought of as inherited diseases caused by a defect in a single gene. While “typi ...
abstracts
... Prunus and Arabidopsis, but conserved regions have been detected including 23% of the Prunus genetic distance and 17% of the Arabidopsis total sequence. The position of more than 30 major genes has been established in the reference map of this genus using data from different populations that are anc ...
... Prunus and Arabidopsis, but conserved regions have been detected including 23% of the Prunus genetic distance and 17% of the Arabidopsis total sequence. The position of more than 30 major genes has been established in the reference map of this genus using data from different populations that are anc ...
Analysis of Biological Networks: Genetic Interaction Networks
... a reduction in growth rate, while KO of both genes results with a non-additive reduction in growth rate (i.e, the double KO reduction is lower then the sum of two single KO reductions). In epistatic interactions, the double KO phenotype will exhibit a growth rate reduction that equals the outcome of ...
... a reduction in growth rate, while KO of both genes results with a non-additive reduction in growth rate (i.e, the double KO reduction is lower then the sum of two single KO reductions). In epistatic interactions, the double KO phenotype will exhibit a growth rate reduction that equals the outcome of ...
Population Differences in Transcript
... Gene expression quantitative trait loci (eQTL) are useful for identifying single nucleotide polymorphisms (SNPs) associated with diseases. At times, a genetic variant may be associated with a master regulator involved in the manifestation of a disease. The downstream target genes of the master regul ...
... Gene expression quantitative trait loci (eQTL) are useful for identifying single nucleotide polymorphisms (SNPs) associated with diseases. At times, a genetic variant may be associated with a master regulator involved in the manifestation of a disease. The downstream target genes of the master regul ...
Matthew Kwong - GEP Community Server
... Annotation projects involving multiple species of the same genus are important for understanding genomic evolution, adaptation, and conserved function. The Drosophila 12 Genomes Consortium paper titled, "Evolution of genes and genomes on the Drosophila phylogeny" demonstrates the wealth of knowledge ...
... Annotation projects involving multiple species of the same genus are important for understanding genomic evolution, adaptation, and conserved function. The Drosophila 12 Genomes Consortium paper titled, "Evolution of genes and genomes on the Drosophila phylogeny" demonstrates the wealth of knowledge ...
Full Article - Medical Ultrasonography Journal
... with a maximum of 800 mg in 24 hours. Therapy is generally managed in one to six monthly cycles, separated by free intervals of 10-14 days. The most feared secondary effects of therapy with Albendazole are considered to be hepatic and haematological toxicity. Patients receiving therapy with Albendaz ...
... with a maximum of 800 mg in 24 hours. Therapy is generally managed in one to six monthly cycles, separated by free intervals of 10-14 days. The most feared secondary effects of therapy with Albendazole are considered to be hepatic and haematological toxicity. Patients receiving therapy with Albendaz ...
Bayesian Partition Models for Identifying Expression Quantitative
... of Pr(Y|X). Motivated by the success of BEAM, Zhang et al. (2010) developed a Bayesian partition (BP) model for eQTL studies based on a joint model of gene expression and SNPs. More specifically, correlated expression traits Y and their associated set of markers X are treated as a module in the BP m ...
... of Pr(Y|X). Motivated by the success of BEAM, Zhang et al. (2010) developed a Bayesian partition (BP) model for eQTL studies based on a joint model of gene expression and SNPs. More specifically, correlated expression traits Y and their associated set of markers X are treated as a module in the BP m ...
Selective regain of egfr gene copies in CD44+/CD24
... reproduction in any medium, provided the original work is properly cited. ...
... reproduction in any medium, provided the original work is properly cited. ...
Virulence gene regulation in Salmonella enterica
... gastroenteritis is much higher (2). Salmonella infection follows ingestion of contaminated food, water or beverages and requires survival in the stomach and colonization of the small intestine. At this stage, the bacteria are seen to start multiplying and adhering to the intestinal mucosa. In the mo ...
... gastroenteritis is much higher (2). Salmonella infection follows ingestion of contaminated food, water or beverages and requires survival in the stomach and colonization of the small intestine. At this stage, the bacteria are seen to start multiplying and adhering to the intestinal mucosa. In the mo ...
Your Health Matters Hormone Therapy for Prostate Cancer – A
... hormone treatment for prostate cancer. The main reasons for intermittent rather than continuous treatment include: • Improved quality of life for the patient—more time off hormone therapy to limit the side effects. • The possibility of extending the time the hormone therapy drugs are effective—in ...
... hormone treatment for prostate cancer. The main reasons for intermittent rather than continuous treatment include: • Improved quality of life for the patient—more time off hormone therapy to limit the side effects. • The possibility of extending the time the hormone therapy drugs are effective—in ...
Gene Expression Microarray Analysis of Archival FFPE Samples
... formalin-fixed paraffin-embedded (FFPE) tissues are the only samples available. To work with these difficult samples, Agilent has developed an FFPE sample protocol optimized for use with Agilent gene expression microarrays. To test the effectiveness of this protocol, total RNA was extracted from a q ...
... formalin-fixed paraffin-embedded (FFPE) tissues are the only samples available. To work with these difficult samples, Agilent has developed an FFPE sample protocol optimized for use with Agilent gene expression microarrays. To test the effectiveness of this protocol, total RNA was extracted from a q ...
Genetic and Physical Mapping of a Type 1 Diabetes Susceptibility
... Genotyping. Microsatellite repeats were genotyped using radioactive labeling of polymerase chain reaction (PCR) primers and denaturing polyacrylamide gel electrophoresis as previously reported (29,33,44). In each case, forward primers were end-labeled using [ -32P]ATP and T4 polynucleotide kinase. P ...
... Genotyping. Microsatellite repeats were genotyped using radioactive labeling of polymerase chain reaction (PCR) primers and denaturing polyacrylamide gel electrophoresis as previously reported (29,33,44). In each case, forward primers were end-labeled using [ -32P]ATP and T4 polynucleotide kinase. P ...
Identification and quantification of mycotoxigenic fungi
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
ABCA3 Gene Mutations in Newborns with Fatal
... The genes for ATP-binding cassette (ABC) transporters encode membrane proteins involved in the transport of compounds across biologic membranes, and 14 ABC genes have been associated with distinct genetic diseases in humans.3 Several ABC transporters are involved in the transport of phospholipids an ...
... The genes for ATP-binding cassette (ABC) transporters encode membrane proteins involved in the transport of compounds across biologic membranes, and 14 ABC genes have been associated with distinct genetic diseases in humans.3 Several ABC transporters are involved in the transport of phospholipids an ...
Epilepsy Update - Epilepsy Ireland
... • Specialist clinics regional • Integrated – Care pathways – Data management ...
... • Specialist clinics regional • Integrated – Care pathways – Data management ...
The aetiology and management of Castleman disease at 50 years
... discrepancy between these studies is difficult to reconcile, but may be attributable to differences in methodology. The study by Soulier et al (1995a), used PCR on fresh-frozen lymph nodes and the series reported by Kikuta et al (1997) used fresh PBMCs, while the others relied on preserved specimens ...
... discrepancy between these studies is difficult to reconcile, but may be attributable to differences in methodology. The study by Soulier et al (1995a), used PCR on fresh-frozen lymph nodes and the series reported by Kikuta et al (1997) used fresh PBMCs, while the others relied on preserved specimens ...
How I manage patients with acquired haemophilia A
... shown similar efficacy, it is reasonable to switch to whichever agent has not yet been used. The decision to deem a particular treatment ineffective will be primarily dependent on the severity of bleeding. If bleeding is life threatening, we think it may be reasonable to decrease the threshold for c ...
... shown similar efficacy, it is reasonable to switch to whichever agent has not yet been used. The decision to deem a particular treatment ineffective will be primarily dependent on the severity of bleeding. If bleeding is life threatening, we think it may be reasonable to decrease the threshold for c ...
BROWSING GENES AND GENOMES WITH ENSEMBL
... 8 Expand the ‘ORTHOLOGS’ section by clicking on the + box. 8 Select ‘Mouse Ensembl Gene ID’ and ‘Homology Type’. 8 Click the [Results] button on the toolbar. 8 Select ‘View All rows as HTML’ or export all results to a file. Tick the box ‘Unique results only’. Your results should show that for mo ...
... 8 Expand the ‘ORTHOLOGS’ section by clicking on the + box. 8 Select ‘Mouse Ensembl Gene ID’ and ‘Homology Type’. 8 Click the [Results] button on the toolbar. 8 Select ‘View All rows as HTML’ or export all results to a file. Tick the box ‘Unique results only’. Your results should show that for mo ...
599 KB - CSIRO Publishing
... end of the human Y (Simpson et al. 1987). In fact, HYA turns out to be an amalgam of products, different in different animals; any product of a widely expressed Y gene will contribute malespecific determinants. The hunt for TDF really revved up in the 1980s, when it became at least theoretically poss ...
... end of the human Y (Simpson et al. 1987). In fact, HYA turns out to be an amalgam of products, different in different animals; any product of a widely expressed Y gene will contribute malespecific determinants. The hunt for TDF really revved up in the 1980s, when it became at least theoretically poss ...
Spinal muscular atrophy: diagnosis, treatment and future prospects R A
... than arms and arms are more affected than the face and diaphragm.8,12 In other words, muscle weakness and atrophy does not have a homogeneous distribution. The severity of muscle weakness is related to age at onset and children with the most severe form of the disease (Type I SMA) can appear normal ...
... than arms and arms are more affected than the face and diaphragm.8,12 In other words, muscle weakness and atrophy does not have a homogeneous distribution. The severity of muscle weakness is related to age at onset and children with the most severe form of the disease (Type I SMA) can appear normal ...
Gene therapy
Gene therapy is the therapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease. Gene therapy could be a way to fix a genetic problem at its source. The polymers are either expressed as proteins, interfere with protein expression, or possibly correct genetic mutations.The most common form uses DNA that encodes a functional, therapeutic gene to replace a mutated gene. The polymer molecule is packaged within a ""vector"", which carries the molecule inside cells.Gene therapy was conceptualized in 1972, by authors who urged caution before commencing human gene therapy studies. By the late 1980s the technology had already been extensively used on animals, and the first genetic modification of a living human occurred on a trial basis in May 1989 , and the first gene therapy experiment approved by the US Food and Drug Administration (FDA) occurred on September 14, 1990, when Ashanti DeSilva was treated for ADA-SCID. By January 2014, some 2,000 clinical trials had been conducted or approved.Early clinical failures led to dismissals of gene therapy. Clinical successes since 2006 regained researchers' attention, although as of 2014, it was still largely an experimental technique. These include treatment of retinal disease Leber's congenital amaurosis, X-linked SCID, ADA-SCID, adrenoleukodystrophy, chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia (ALL), multiple myeloma, haemophilia and Parkinson's disease. Between 2013 and April 2014, US companies invested over $600 million in the field.The first commercial gene therapy, Gendicine, was approved in China in 2003 for the treatment of certain cancers. In 2011 Neovasculgen was registered in Russia as the first-in-class gene-therapy drug for treatment of peripheral artery disease, including critical limb ischemia.In 2012 Glybera, a treatment for a rare inherited disorder, became the first treatment to be approved for clinical use in either Europe or the United States after its endorsement by the European Commission.