BCB 444/544
... find more divergent sequences. Based on the E-values, the first 14 hits from both (which are the same 14 hits found by using the BLOSUM62 matrix) are very likely to be related to our query sequence, while the other hits may or may not be. Because the E-values are high (>1) for hits after top ranking ...
... find more divergent sequences. Based on the E-values, the first 14 hits from both (which are the same 14 hits found by using the BLOSUM62 matrix) are very likely to be related to our query sequence, while the other hits may or may not be. Because the E-values are high (>1) for hits after top ranking ...
TINF2 Pulmonary fibrosis associated with gene mutation: is somatic reversion required?
... dyskeratosis congenita, pulmonary fibrosis and heterozygous mutation in TINF2 (telomerase repeat binding factor 1-interacting nuclear factor 2). TIN2, the TINF2 gene product, TERT (telomere reverse transcriptase) and TERC (telomerase RNA component) participate in the regulation of telomere elongatio ...
... dyskeratosis congenita, pulmonary fibrosis and heterozygous mutation in TINF2 (telomerase repeat binding factor 1-interacting nuclear factor 2). TIN2, the TINF2 gene product, TERT (telomere reverse transcriptase) and TERC (telomerase RNA component) participate in the regulation of telomere elongatio ...
The Quest for Immortality
... evade death and of immortal beings. We have always had a fear of death and have remained fascinated with the subject since Sumerian times. We think about living forever and we contemplate escaping death. Alchemical elixirs, endless reincarnation, eternal afterlives, and personified death serve as a ...
... evade death and of immortal beings. We have always had a fear of death and have remained fascinated with the subject since Sumerian times. We think about living forever and we contemplate escaping death. Alchemical elixirs, endless reincarnation, eternal afterlives, and personified death serve as a ...
Introduction to Genetics WINTER 2017 EXAM I 1. In one strand of
... H U G E are four dominant genes controlling tomato size that are each located on different chromosomes. A cross is carried out between Plant 1 with genotype- Hh Uu Gg ee and Plant 2 of genotype- hh Uu Gg Ee. 27. What percentage of the progeny are expected to be phenotypically identical to Plant 1? A ...
... H U G E are four dominant genes controlling tomato size that are each located on different chromosomes. A cross is carried out between Plant 1 with genotype- Hh Uu Gg ee and Plant 2 of genotype- hh Uu Gg Ee. 27. What percentage of the progeny are expected to be phenotypically identical to Plant 1? A ...
Resilience and Frailty in Old Age: What Drives it?
... Aging is associated with up‐regulation of immune function genes. Transcriptome‐wide meta‐analysis of genes whose expression differs by age in 7,257 individuals of European ancestry. Findings replicated in another 8,009 individuals. 1,497 genes were differentially expressed with age. The major clus ...
... Aging is associated with up‐regulation of immune function genes. Transcriptome‐wide meta‐analysis of genes whose expression differs by age in 7,257 individuals of European ancestry. Findings replicated in another 8,009 individuals. 1,497 genes were differentially expressed with age. The major clus ...
Ponce de Leon and the Telomere of Youth
... span, and without them we could all live in good health until our MLPth birthday.2 Aging research might not make us immortal, but we’d at least be able to have a well-planned Last Supper. Instead, and more plausibly, recent mortality data show that death rates do not rapidly accelerate at very old a ...
... span, and without them we could all live in good health until our MLPth birthday.2 Aging research might not make us immortal, but we’d at least be able to have a well-planned Last Supper. Instead, and more plausibly, recent mortality data show that death rates do not rapidly accelerate at very old a ...
striking similarities in diverse telomerase proteins revealed by
... Telomerase is a promising target for cancer therapy because it is generally present in very low levels in normal somatic cells, but it is highly active in many human malignancies [11]. Telomerase targeting strategies have included short interfering RNA (siRNA) knockdown of endogenous hTER and a comb ...
... Telomerase is a promising target for cancer therapy because it is generally present in very low levels in normal somatic cells, but it is highly active in many human malignancies [11]. Telomerase targeting strategies have included short interfering RNA (siRNA) knockdown of endogenous hTER and a comb ...
Read the Nobel Lecture
... suggested that a linear chromosome should get shorter as cells divided, based on the mechanism by which DNA polymerase replicates DNA during cell division (Watson, 1972). A similar idea was also proposed by Alexi Olovinkov, who suggested that chromosome ends might shorten after rounds of DNA replica ...
... suggested that a linear chromosome should get shorter as cells divided, based on the mechanism by which DNA polymerase replicates DNA during cell division (Watson, 1972). A similar idea was also proposed by Alexi Olovinkov, who suggested that chromosome ends might shorten after rounds of DNA replica ...
PLoS One
... Arabidopsis plants induce a SOG1-dependent programmed endoreduplicative response to DNA double strand breaks [31]. To test for an equivalent response to telomeric damage, we used flow cytometry to carry out ploidy analysis on nuclei of seven-dayold WT, tertG2 and tertG7 plantlets. The results of thi ...
... Arabidopsis plants induce a SOG1-dependent programmed endoreduplicative response to DNA double strand breaks [31]. To test for an equivalent response to telomeric damage, we used flow cytometry to carry out ploidy analysis on nuclei of seven-dayold WT, tertG2 and tertG7 plantlets. The results of thi ...
Comparative Studies of Apoptosis in Xenopus laevis and
... DNA, thus initiating transcription of an array of genes that expand the functionality of the p53. The same phosphorylated activation process of Xp53 will occur in X. laevis cells (3). In mammalian cells, p53 can be inhibited as a consequence of being bound to Mdm2. In Xenopus cells, its equivalent ( ...
... DNA, thus initiating transcription of an array of genes that expand the functionality of the p53. The same phosphorylated activation process of Xp53 will occur in X. laevis cells (3). In mammalian cells, p53 can be inhibited as a consequence of being bound to Mdm2. In Xenopus cells, its equivalent ( ...
PDF
... decide to combine the dut1 mutation with mutations in various genes known to be involved in DNA repair. Most of the cells carrying these pairs of mutations do not have a phenotype that is significantly more severe than the parent strains carrying the individual mutations. However, you find two genes ...
... decide to combine the dut1 mutation with mutations in various genes known to be involved in DNA repair. Most of the cells carrying these pairs of mutations do not have a phenotype that is significantly more severe than the parent strains carrying the individual mutations. However, you find two genes ...
Chapter 18 Cancer Genetics and Genomics
... D. activation of a proto-oncogene by a virus. 32. Matthew has the inherited form of the eye cancer retinoblastoma (RB). His disease is caused by A. a germinal mutation in one RB allele and no mutation in the other allele. B. a somatic mutation in each copy of the RB gene in the same cell. C. a germi ...
... D. activation of a proto-oncogene by a virus. 32. Matthew has the inherited form of the eye cancer retinoblastoma (RB). His disease is caused by A. a germinal mutation in one RB allele and no mutation in the other allele. B. a somatic mutation in each copy of the RB gene in the same cell. C. a germi ...
Mechanisms of cell aging in cell culture
... posttranslational modifications may be defective [24]. ...
... posttranslational modifications may be defective [24]. ...
Cell senescence questions
... shortening telomeres make the cell know that it has undergone plenty of divisions) Senescence is a broader category. Rep. senescence is an example of cellular senescence. Cellular senescence refers to the cell response to any of many stimuli. (cell division/shortening telomeres is just one of these ...
... shortening telomeres make the cell know that it has undergone plenty of divisions) Senescence is a broader category. Rep. senescence is an example of cellular senescence. Cellular senescence refers to the cell response to any of many stimuli. (cell division/shortening telomeres is just one of these ...
supplementary material
... RNA isolated from skin, mouse embryonic fibroblasts, or keratinocytes were reversetranscribed using Superscript II (Invitrogen) as per manufacturer’s protocol. Each cDNA sample was then analyzed by quantitative PCR using the iCycler thermocycler machine (BioRad). PCR was performed in a reaction volu ...
... RNA isolated from skin, mouse embryonic fibroblasts, or keratinocytes were reversetranscribed using Superscript II (Invitrogen) as per manufacturer’s protocol. Each cDNA sample was then analyzed by quantitative PCR using the iCycler thermocycler machine (BioRad). PCR was performed in a reaction volu ...
CELL CYCLES: MITOSIS, CYTOKINESIS AND MEIOSIS
... If there are 10 chromosomes in a plant cell at G1 phase, how many chromatids will there be at the beginning of the next M phase? (a) 5 (b) 10 (c) 20 (d) 40 ...
... If there are 10 chromosomes in a plant cell at G1 phase, how many chromatids will there be at the beginning of the next M phase? (a) 5 (b) 10 (c) 20 (d) 40 ...
Assembly of Mutant-Template Telomerase RNA into Catalytically
... of telomerase may signal cellular senescence (3, 4). In contrast, highly active telomerase is a common feature of a wide variety of human cancers (5). Attempts to attenuate telomerase function in cultured human cancer cells have led to telomere shortening (6) and, eventually, to cellular apoptosis, ...
... of telomerase may signal cellular senescence (3, 4). In contrast, highly active telomerase is a common feature of a wide variety of human cancers (5). Attempts to attenuate telomerase function in cultured human cancer cells have led to telomere shortening (6) and, eventually, to cellular apoptosis, ...
Rapid method of total RNA mini-preparation from eucaryotic cells
... discarded and cells were washed once with 1 ml of 0.9% sodium chloride solution. Then 200 jtl of lithium chloride/urea solution (3 M LiCl, 6 M urea) (3) were added to each well and incubated for 5 min at 20°C. This was followed by addition of 1/10 vol. of 3 M sodium acetate solution and of 2.5 vol o ...
... discarded and cells were washed once with 1 ml of 0.9% sodium chloride solution. Then 200 jtl of lithium chloride/urea solution (3 M LiCl, 6 M urea) (3) were added to each well and incubated for 5 min at 20°C. This was followed by addition of 1/10 vol. of 3 M sodium acetate solution and of 2.5 vol o ...
Cell Aging
... damage and genetically determined events or genome crisis. • Senescence - Phenotypes of aging are caused by an increase in frequency of senescent cells. Senescence may be the result of telomere loss (replicative senescence) or cell stress (cellular senescence). ...
... damage and genetically determined events or genome crisis. • Senescence - Phenotypes of aging are caused by an increase in frequency of senescent cells. Senescence may be the result of telomere loss (replicative senescence) or cell stress (cellular senescence). ...
Cancer Prone Disease Section Dyskeratosis congenita (DKC) Atlas of Genetics and Cytogenetics
... The major part of patients di before 20 years, mainly from infectious complications of immune deficiency. 90% of patients have haematological abnormalities when 30 year-old, and bone marrow failure is the main cause of early morbidity in 71% of cases. It can evolve toward aplastic anemia or myelodys ...
... The major part of patients di before 20 years, mainly from infectious complications of immune deficiency. 90% of patients have haematological abnormalities when 30 year-old, and bone marrow failure is the main cause of early morbidity in 71% of cases. It can evolve toward aplastic anemia or myelodys ...
TheScienceofSuperAmber
... called helicases unwind the DNA helix to facilitate the advance of sequence-reading enzymes (DNA polymerase) that build strands complementary to those that were unwound. During replication the DNA can only be read in the 3’ (read as “three prime”) to 5’ direction (It’s more correct to say that the ...
... called helicases unwind the DNA helix to facilitate the advance of sequence-reading enzymes (DNA polymerase) that build strands complementary to those that were unwound. During replication the DNA can only be read in the 3’ (read as “three prime”) to 5’ direction (It’s more correct to say that the ...
PowerPoint ******
... binding sites to proteins, especially in the domain of metal-based drugs. • It should ultimately provide data that facilitate drug design and discovery. ...
... binding sites to proteins, especially in the domain of metal-based drugs. • It should ultimately provide data that facilitate drug design and discovery. ...
Slide 1
... Additionally, G4 forming sequences are overrepresented in proto-oncogenes and underrepresented in tumor suppressor genes (Eddy, 2006). ...
... Additionally, G4 forming sequences are overrepresented in proto-oncogenes and underrepresented in tumor suppressor genes (Eddy, 2006). ...
Product Datasheet TERT Antibody NB110
... Telomerase reverse transcriptase antibody used as stem/progenitor cell marker in ICC-IF application on human corneal endothelial cells grown with serial explant culture technique and explant culture wounding model. Bandhakavi S, Kim YM, Ro SH et al. Quantitative nuclear proteomics identifies mTOR re ...
... Telomerase reverse transcriptase antibody used as stem/progenitor cell marker in ICC-IF application on human corneal endothelial cells grown with serial explant culture technique and explant culture wounding model. Bandhakavi S, Kim YM, Ro SH et al. Quantitative nuclear proteomics identifies mTOR re ...
No Slide Title
... Endurance exercise can increase oxygen utilization from 10 to 20 times over the resting state. This greatly increases the generation of free radicals, prompting concern about enhanced damage to muscles and other tissues. The question that arises is, how effectively can athletes defend against the in ...
... Endurance exercise can increase oxygen utilization from 10 to 20 times over the resting state. This greatly increases the generation of free radicals, prompting concern about enhanced damage to muscles and other tissues. The question that arises is, how effectively can athletes defend against the in ...
Telomerase
Telomerase also called telomere terminal transferase is a ribonucleoprotein that adds the nucleotide ""TTAGGG"" to the 3' end of telomeres, which are found at the ends of eukaryotic chromosomes. A telomere is a region of repetitive sequences at each end of a chromatid, which protects the end of the chromosome from deterioration or from fusion with neighbouring chromosomes.Telomerase is a reverse transcriptase enzyme that carries its own RNA molecule (with the pattern of ""CCCAAUCCC"" in vertebrates), which is used as a template when it elongates telomeres.