Download 4-14

BIOL 3301
GENETICS
Date: Monday, April 14, 1.00-2.30 pm
Subject: Gene mutation.
Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition)
Chapter 15: Gene mutation
________________________________________________________________________
Key concepts:
How DNA changes affect phenotype (15-1, 15-2)
-
base substitutions: transitions, transversions
silent mutations, missense (synonymous & non-synonymous), nonsense, null, leaky
base additions or deletions: frameshift mutations
Somatic versus germinal mutation
-
somatic mutation, clone (15-5)
germinal mutation (15-8)
Mutant types (15-9, 15-11, 15-12)
-
morphological, lethal, conditional (restrictive/permissive), biochemical
(prototropic/auxotrophic), loss-of-function, gain-of-function
Occurrence of mutations
-
genetic screens (15-13), mutation rate/frequency (15-16, Table 15-3)
Selective systems
-
reversion of auxotrophs (15-19)
filtration enrichment (15-20)
resistance (15-22, 15-23)
Mutation and cancer (15-27)
____________________________________________________________
Homework: ________________________________________________________________________
Reading for next class (4-16):
Chapter 16: Mechanisms of gene mutation