* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
Microbial metabolism wikipedia , lookup
Western blot wikipedia , lookup
Signal transduction wikipedia , lookup
Mitogen-activated protein kinase wikipedia , lookup
Oxidative phosphorylation wikipedia , lookup
Metalloprotein wikipedia , lookup
Ultrasensitivity wikipedia , lookup
Gene regulatory network wikipedia , lookup
Isotopic labeling wikipedia , lookup
Photosynthetic reaction centre wikipedia , lookup
Paracrine signalling wikipedia , lookup
Phosphorylation wikipedia , lookup
Biochemistry wikipedia , lookup
Evolution of metal ions in biological systems wikipedia , lookup
Citric acid cycle wikipedia , lookup
Glyceroneogenesis wikipedia , lookup
Enzyme inhibitor wikipedia , lookup
Metabolomics wikipedia , lookup
Biosynthesis wikipedia , lookup
Biochemical cascade wikipedia , lookup
Amino acid synthesis wikipedia , lookup
Basal metabolic rate wikipedia , lookup
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY FOURTH SEMESTER – April 2009 BT 4951 - METABOLIC ENGINEERING Date & Time: 23/04/2009 / 9:00 - 12:00 Dept. No. Max. : 100 Marks SECTION A (20 marks) Answer all the questions I. Choose the best answer (5X1=5) 1. Energy required to express the house keeping genes are derived from a) Biosynthetic reaction c) Fueling reaction b) Enzymatic reaction d) Hormonal reaction 2. Initial rate of an enzyme catalysed reaction depends on a) Enzyme concentration c) pH of the medium b) Substrate concentration d) Temperature of the medium. 3. A ligand can be a a) Protein b) Lipid c) Carbohydrate d) metal ion 4. Choose the carbon source which enters the TCA cycle. a) Glucose b) Acetyl coA c) Pyruvate d) Citrate 5. Name the enzyme for which insulin is not an inducer a) Phosphofructokinase c) Glucose-6-Phosphatase b) Pyruvate kinase d) Glycogen synthetase II. State True or False; if false give reasons (5X1=5) 6. Primary metabolism synthesizes building block metabolite and energy in uniform amounts. 7. The fluxes in and out of the Black box model are given by a constant rate and a variable substrate and product rate. 8. Recombinant PCR cannot be used for making novel proteins. 9. Strongly weak nodes are insensitive to regulation. 10. Secondary metabolites cannot be overproduced by altering flux. III. Complete the following. (5X1=5) 11. NLS corresponds to _______. 12. Glutamic acid was overproduced in_______. 13. Hsp refers to________. 14. Electronic cell mimicks ________. 15. GOGAT stands for________. 1 VE 46 IV. Answer the following, each about 50 words (5X1=5) 16. Explain Substrate level phosphorylation. 17. What is an irreversible inhibitor? 18. Write about UAS. 19. Define Metabolic Control theory. 20. Explain Gene shuffling. SECTION B Answer any five, each answer within 350 words only. (5X8=40) 21. What are perturbations? How are they helpful in analyzing flux? 22. Describe Pentose phosphate pathway and its regulation. 23. Give a schematic representation of various types of enzyme inhibition. 24. What are secondary metabolites? Discuss its production in a plant cell. 25. What are the criteria to be employed in the choice of an organism for metabolite overproduction? 26. Give a blueprint for the selection of induced mutants synthesizing ethanol from 27. What are the potentials and perils of Metabolic engineering? 28. Discuss the glycolysis pathway and its regulation in Corynebaterium glutamicum. overproducing Lysine. xylose. SECTION C Answer the following, each in about 1500 words. (2X20=40) 29 a) Elaborate the classification of nodes and highlight the various strategies adopted for accelerating primary metabolism. (Or) b) Outline the pathway of TCA cycle and MFA of a plant cell. 30 a) Give a detailed description on Protein Engineering and discuss the various methodologies followed. (Or) b) Write about Signal Transduction and the pathways involved. ************** 2