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Transcript
Figure 7.4 Heart regeneration in the zebrafish. (A) Longitudinal section through an intact heart. ba, bulbus arteriosus. (B) Heart after amputation of 20% of ventricle. (C) Higher magnification of unamputated ventricular apex, showing the level of amputation. (D) One day post-amputation, showing plasma clot filled with erythrocytes (arrowheads). (EāH) The heart muscle is stained for myosin heavy chain (brown) and fibrin (blue). The fibrin disappears and is replaced by new cardiac muscle. (I) Area of ventricle as measured from largest section after removal of ā20% of the ventricle (0 days). By 60 days the area of the section has been restored to normal. Numbers in parentheses = number of hearts examined. Asterisk indicates statistical significance compared to uncut. Reproduced with permission from Poss et al., Heart regeneration in zebrafish. Science 298:2188ā2190. Copyright 2002, AAAS. Copyright © 2012 Elsevier Inc. All rights reserved.