Download Reading the neural code in behaving animals, ~1000 neurons at a ,me

ADVANCED INSTRUMENTATION SEMINARS March 5, 2015!
1:30 PM!
Kavli 3rd floor!
Reading the neural code in behaving animals, ~1000 neurons at a 3me GUEST SPEAKER –!
MARK SCHNITZER!
Stanford University A longstanding challenge in neuroscience is to understand how popula3ons of individual neurons and glia contribute to animal behavior and brain disease. Addressing this challenge has been difficult partly due to lack of appropriate brain imaging technology for visualizing cellular proper3es in awake behaving animals. I will describe a miniaturized, integrated fluorescence microscope for imaging cellular dynamics in the brains of freely behaving mice. The microscope also allows 3me-­‐lapse imaging, for watching how individual cells' coding proper3es evolve over weeks. By using the integrated microscope to perform calcium-­‐imaging in behaving mice as they repeatedly explored a familiar environment, we tracked the place fields of thousands of CA1 hippocampal neurons over weeks. Rewarded spa3al loca3ons were represented by a dispropor3onally greater number of place cells than non-­‐rewarded loca3ons. Spa3al coding was also highly dynamic, for on each day the neural representa3on of this environment involved a unique subset of neurons. Yet, the cells within the ~15–25% overlap between any two of these subsets retained the same place fields, and though this overlap was also dynamic it sufficed to preserve a stable and accurate ensemble representa3on of space across weeks. This study in CA1 illustrates the types of 3me-­‐lapse studies on reward, memory, ensemble neural dynamics, and coding that will now be possible in mul3ple brain regions of behaving rodents. Ongoing brain areas under study include amygdala, basal ganglia and mul3ple others. http://www-group.slac.stanford.edu/ais!